Biotreatment of anthraquinone dye Drimarene Blue K2RL

Drimarene Blue (Db) K2RL is a reactive anthraquinone dye, used extensively in textile industry, due to poor adsorbability to textile fiber; it has a higher exhaustion rate in wastewater. The dye is toxic, carcinogenic, mutagenic and resistant to degradation. Decolorization of this dye was studied in two different systems. Shake flask study, using the same immobilized fungus Aspergillus niger SA1 with increasing concentration (10 - 300 ppm) of dye resulted in 75% decolorization in 24 h with 10 ppm concentration, while the higher the concentration of dye, the lower the values (68% at 25 ppm, 40% at 50 ppm, 11% at 100 ppm, 3% at 200 ppm and 2% at 300 ppm) of decolorization (p < 0.05). Simulated textile effluent containing 10 ppm dye Db K2RL was tested aerobically using sludge in stirred tank reactor (STR) at 30ºC for 8 days. The values achieved of chemical oxygen demand (COD) reduction and decolorization were 84% (r = 0.968) and 30% (r = 0.905), respectively. This study revealed that immobilized fungus A. niger SA1 have potential, while sludge containing bacterial consortium have limited potential to be used as bioremediation for textile dye Drimarene Blue K2RL

Up-flow immobilized fungal Column Reactor for the Treatment of Anthraquinone dye Drimarene blue Ksub>2RL

This research work is on the decolorization of a reactive anthraquinone dye Drimarene blue (Db) K2RL, which is known for its markedly usage in textile industry. Due to poor adsorbability to textile fiber, it has a higher exhaustion rate in wastewater. The main objective of our research work was to evaluate the potential of an Up-flow Column reactor (UFCR) (13x1.7//) containing the fungal strain, Aspergillus niger SA1 (immobilized on support material Scotch-BriteTM) for the decolorization of a dye, Db K2RL, insimulated textile effluent. Different concentrations of dye in the effluent were treated in the reactor for 24 h, with a flow rate of 10 mL-1 at hydraulic retention time (HRT) of 10 h. Using anoxic UFCR, decolorization of the effluent was observed maximum, that is, 94.26% at 10 ppm of dye; however, it reduced to 58.51% at 300 ppm of dye. A trend towards increase ( 15%) in decolorization of effluent was noted, when the effluent was aerated prior to treatment. Recycling of the effluent containing dye increased the decolorization (85% at 130 ppm of dye; 66% at 500 ppm of dye), however, further recycling decreased the rate of decolorization, which might be due to desorption by the immobilizedfungus. The results of these findings providing important insights into the development of effective treatment technology for bioremediation of textile dyes

Exposure to NO2 in occupational built environments in urban centre in Lahore

Increased economic growth, urbanisation and substantial rise in automobile vehicles has contributed towards the elevated levels of air pollution in major cities in Pakistan. Aone week study was conducted by using passive samplers to assess NO2 concentration in occupational built environments at two most congested and populated sites of Lahore. Both sites were locatedon the busy roads of Lahore. At Site-I the highest concentration was in outdoors followed by corridor and indoor. While at Site II all the sampling location wereindoors and level were comparable to that of outdoor levelsat Site I. The results suggest the likely contribution of ambient sources in exposure to indoor NO2 in educational and other occupational built environments in urban centres

Exposure to NO<inf>2</inf> in occupationalbuilt environmnets in urban centre in Lahore

Increased economic growth, urbanisation and substantial rise in automobile vehicles has contributed towards the elevated levels of air pollution in major cities in Pakistan. Aone week study was conducted by using passive samplers to assess NO2 concentration in occupational built environments at two most congested and populated sites of Lahore. Both sites were locatedon the busy roads of Lahore. At Site-I the highest concentration was in outdoors followed by corridor and indoor. While at Site II all the sampling location wereindoors and level were comparable to that of outdoor levelsat Site I. The results suggest the likely contribution of ambient sources in exposure to indoor NO2 in educational and other occupational built environments in urban centres

A Qualitative Comparison of ANSYS and OpenFOAM results for Carbon dioxide Plume Transport

This research presents Computational Fluid Dynamics (CFD) simulations in ANSYS® illustrating emissions of to the air. The CFD simulations is employed to study plume transport in urban environment, i.e., Breivika port in the city of Tromsø. The case study presents a two-phase model considering specific wind strength and direction in the city of Tromsø. Geographical coordinates, temperature, and wind data were obtained from the open sources, such as Google Maps, and Norwegian Meteorological Institute. The results from the simulations indicates a potential outcome with respect to various weather conditions. It was revealed for vessels less than 30 meter chimney height, the higher the wind strength, the lower the plume dispersion, causing the plume to stay closer to the terrain. This brings in a concentrated amount of pollutants closer to the public areas. The terrain in the model is recognizable for the Tromsø port’s location. From the CFD results, it is illustrated that onshore wind with high wind strength could affect the environment. The results simulated in OpenFOAM are qualitatively showing the same as visible in ANSYS®

Prevalence of hepatitis B and C and assessment of responsible risk factors among the vulnerable β-thalassemic patients of Azad Kashmir, Pakistan

Approximately 350 million patients of hepatitis B and 170 million patients of Hepatitis C are present worldwide according to WHO. Many risk factors are involved in the transmission of theses deadly viral infections but blood transfusion in Beta thalassemic patients is working with two faces, one as remedy and the other is key risk factor in the spread of silent killers. Thalassemia patients registered in Combine Military Hospital (CMH) Rawalakot and Sheikh Khalifa Bin Zayed Al-Nahyan Hospital, Muzaffarabad Azad Jammu and Kashmir Pakistan were studied for the viral hepatitis B and C prevalence. A total of 303 (including 164 males and 139 females) individuals, aged between 1 and 12 years were studied. All the understudy participants were interviewed through questionnaire method. After taking written consent from each participant or guardian, 5 ml of blood was collected from each participant and brought to the working laboratory for HBV and HCV screening through ICT kit method. All ICT positive samples were further confirmed through ELISA. Individuals 25(8.2%) were found positive for both hepatitis B surface Antigen (HBsAg) and Anti hepatitis C antibody (Anti-HCV antibody) after initial screening with no coinfection of both diseases. Out of 25 total infected individuals, 05(1.6%) were found HBsAg positive and 20(6.6%) were found anti-HCV positive. All the ICT positive individuals were further confirmed by quantitative Enzyme Linked Immunosorbent Assay (ELISA) and 23(7.6%) individuals were confirmed for both hepatitis B and C including 05(1.6%) HBsAg positive as well as 18(5.9%) anti-HCV antibody positive individuals. We can conclude that 8.2% prevalence of hepatitis B and C among thalassemic patients is an alarming health concern which directly indicates to pay attention for ensuring 100% safe blood transfusion

Heavy Metal Levels in Vegetables and Soil Cultivated with Industrial Wastewater from Different Sites of Chunian and Jamber, District, Kasur

In human diet, vegetables play important role to maintain the physiological conditions. Due to anthropogenic activities and pollution, the food items become contaminated. The present study was performed to evaluate the level of heavy metals in the vegetables irrigated with wastewater across Chunian and Jamber, district, Kasur. Level of heavy metals from the study area like Zinc, Lead and chromium in the soil, water and vegetables was compared. The four sites of each city and 10 vegetables e.g. potato, radish, carrot, fenugreek, spinach, tomato, Onion, Turnip, Cauliflower, Pangalo were selected to conduct the experiment. The vegetables were irrigated with industrial wastewater and the concentration of heavy metals was measured by the atomic absorption spectrophotometer (AAS). We concluded that the level of heavy metals was beyond the FAO limits in irrigated water due to industrial waste. In Jamber and Chunian, the level of Zn and Pb was high and beyond the FAO safe limits in the all water sample, the level of Cr was much higher only in the water sample of one site from Jamber. The concentration of zinc was higher in soil samples as compared to lead and chromium. Zn and Pb in vegetables of study area were labeled as priority pollutants but this concentration was within the safe limits set by FAO. However, constant inspection of heavy metals is recommended to avoid accumulation in the food chain and thus avoid human health risks. Keywords: Atomic absorption spectrophotometer, Heavy metals, Industrial wastewater, Vegetables

Automated virtual reality cognitive therapy for people with psychosis: Protocol for a qualitative investigation using peer research methods

BACKGROUND: Many people with psychosis experience difficulties in everyday social situations. Anxiety can make life challenging, leading to withdrawal. Cognitive therapy, using active in vivo learning, enables people to overcome fears. These treatments are not readily available to people with psychosis. Automated virtual reality (VR) therapy is a potential route to increase accessibility. The gameChange automated VR cognitive therapy is designed to help people overcome anxious avoidance and build confidence in everyday social situations. A virtual coach guides the person through the treatment. Understanding user experience is key to facilitating future implementation. Peer research methods, in which people with lived experience of the issues being studied are involved in collecting and analyzing data, may be useful in developing this understanding. This encourages researchers to draw on their lived experience to explore participant perspectives and co-create knowledge. OBJECTIVE: The primary objective is to use a peer research approach to explore the participant experience of a novel automated VR therapy for anxious social avoidance. This includes understanding (1) the experience of anxious social avoidance in people with psychosis, (2) the experience of the gameChange automated VR cognitive therapy, and (3) any potential impact of the therapy in people’s lives. This will inform future implementation strategies. The secondary objective is to explore how peer research can be used to co-create knowledge. METHODS: Semistructured interviews will be conducted with approximately 25 people with psychosis participating in the gameChange trial (ISRCTN17308399). Participants will be recruited from the five trial centers based in National Health Service mental health trusts across England. Interviews will be conducted by two researchers. One is a peer researcher with similar lived experience to the trial participants. The other has lived experiences of mental health issues that do not directly overlap with those of the trial participants. Interview questions will focus on an individual’s experience of anxious social avoidance, experiences of participating in the gameChange VR therapy, and any changes or impact following therapy. The interview schedule was developed in collaboration with the gameChange Lived Experience Advisory Panel (LEAP), comprising 10 project advisors with lived experience of psychosis. Interpretative phenomenological analysis and template analysis will be used to explore individual accounts. The LEAP will contribute to the analysis. RESULTS: Data collection will be conducted from April to September 2021, and analysis will be conducted from June to October 2021. As of September 28, 2021, 20 participants had been interviewed, and coding is underway. CONCLUSIONS: The study, employing a peer research approach, may provide a unique insight into the experiences of anxious social avoidance in people with psychosis and its treatment using automated VR therapy. This will inform potential future implementation of VR automated therapies in mental health services. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/3174

Thalassemia, a human blood disorder

A group of inherited blood defects is known as Thalassemia is among the world’s most prevalent hemoglobinopathies. Thalassemias are of two types such as Alpha and Beta Thalassemia. The cause of these defects is gene mutations leading to low levels and/or malfunctioning α and β globin proteins, respectively. In some cases, one of these proteins may be completely absent. α and β globin chains form a globin fold or pocket for heme (Fe++) attachment to carry oxygen. Genes for alpha and beta-globin proteins are present in the form of a cluster on chromosome 16 and 11, respectively. Different globin genes are used at different stages in the life course. During embryonic and fetal developmental stages, γ globin proteins partner with α globin and are later replaced by β globin protein. Globin chain imbalances result in hemolysis and impede erythropoiesis. Individuals showing mild symptoms include carriers of alpha thalassemia or the people bearing alpha or beta-thalassemia trait. Alpha thalassemia causes conditions like hemolytic anemia or fatal hydrops fetalis depending upon the severity of the disease. Beta thalassemia major results in hemolytic anemia, growth retardation, and skeletal aberrations in early childhood. Children affected by this disorder need regular blood transfusions throughout their lives. Patients that depend on blood transfusion usually develop iron overload that causes other complications in the body systems like renal or hepatic impairment therefore, thalassemias are now categorized as a syndrome. The only cure for Thalassemias would be a bone marrow transplant, or gene therapy with currently no significant success rate. A thorough understanding of the molecular basis of this syndrome may provide novel insights and ideas for its treatment, as scientists have still been unable to find a permanent cure for this deadly disease after more than 87 years since it is first described in 1925

Automated virtual reality therapy to treat agoraphobic avoidance and distress in patients with psychosis (gameChange): a multicentre, parallel-group, single-blind, randomised, controlled trial in England with mediation and moderation analyses

Background: Automated delivery of psychological therapy using immersive technologies such as virtual reality (VR) might greatly increase the availability of effective help for patients. We aimed to evaluate the efficacy of an automated VR cognitive therapy (gameChange) to treat avoidance and distress in patients with psychosis, and to analyse how and in whom it might work. // Methods: We did a parallel-group, single-blind, randomised, controlled trial across nine National Health Service trusts in England. Eligible patients were aged 16 years or older, with a clinical diagnosis of a schizophrenia spectrum disorder or an affective diagnosis with psychotic symptoms, and had self-reported difficulties going outside due to anxiety. Patients were randomly assigned (1:1) to either gameChange VR therapy plus usual care or usual care alone, using a permuted blocks algorithm with randomly varying block size, stratified by study site and service type. gameChange VR therapy was provided in approximately six sessions over 6 weeks. Trial assessors were masked to group allocation. Outcomes were assessed at 0, 6 (primary endpoint), and 26 weeks after randomisation. The primary outcome was avoidance of, and distress in, everyday situations, assessed using the self-reported Oxford Agoraphobic Avoidance Scale (O-AS). Outcome analyses were done in the intention-to-treat population (ie, all participants who were assigned to a study group for whom data were available). We performed planned mediation and moderation analyses to test the effects of gameChange VR therapy when added to usual care. This trial is registered with the ISRCTN registry, 17308399. // Findings: Between July 25, 2019, and May 7, 2021 (with a pause in recruitment from March 16, 2020, to Sept 14, 2020, due to COVID-19 pandemic restrictions), 551 patients were assessed for eligibility and 346 were enrolled. 231 (67%) patients were men and 111 (32%) were women, 294 (85%) were White, and the mean age was 37·2 years (SD 12·5). 174 patients were randomly assigned to the gameChange VR therapy group and 172 to the usual care alone group. Compared with the usual care alone group, the gameChange VR therapy group had significant reductions in agoraphobic avoidance (O-AS adjusted mean difference –0·47, 95% CI –0·88 to –0·06; n=320; Cohen's d –0·18; p=0·026) and distress (–4·33, –7·78 to –0·87; n=322; –0·26; p=0·014) at 6 weeks. Reductions in threat cognitions and within-situation defence behaviours mediated treatment outcomes. The greater the severity of anxious fears and avoidance, the greater the treatment benefits. There was no significant difference in the occurrence of serious adverse events between the gameChange VR therapy group (12 events in nine patients) and the usual care alone group (eight events in seven patients; p=0·37). // Interpretation: Automated VR therapy led to significant reductions in anxious avoidance of, and distress in, everyday situations compared with usual care alone. The mediation analysis indicated that the VR therapy worked in accordance with the cognitive model by reducing anxious thoughts and associated protective behaviours. The moderation analysis indicated that the VR therapy particularly benefited patients with severe agoraphobic avoidance, such as not being able to leave the home unaccompanied. gameChange VR therapy has the potential to increase the provision of effective psychological therapy for psychosis, particularly for patients who find it difficult to leave their home, visit local amenities, or use public transport. // Funding: National Institute of Health Research Invention for Innovation programme, National Institute of Health Research Oxford Health Biomedical Research Centre