Elemental abundances of low-mass stars in nearby young associations: AB Doradus, Carina Near, and Ursa Major

We present stellar parameters and abundances of 11 elements (Li, Na, Mg, Al, Si, Ca, Ti, Cr, Fe, Ni, and Zn) of 13 F6-K2 main-sequence stars in the young groups AB Doradus, Carina Near, and Ursa Major. The exoplanet-host star \iota Horologii is also analysed. The three young associations have lithium abundance consistent with their age. All other elements show solar abundances. The three groups are characterised by a small scatter in all abundances, with mean [Fe/H] values of 0.10 (\sigma=0.03), 0.08 (\sigma=0.05), and 0.01 (\sigma=0.03) dex for AB Doradus, Carina Near, and Ursa Major, respectively. The distribution of elemental abundances appears congruent with the chemical pattern of the Galactic thin disc in the solar vicinity, as found for other young groups. This means that the metallicity distribution of nearby young stars, targets of direct-imaging planet-search surveys, is different from that of old, field solar-type stars, i.e. the typical targets of radial velocity surveys. The young planet-host star \iota Horologii shows a lithium abundance lower than that found for the young association members. It is found to have a slightly super-solar iron abundance ([Fe/H]=0.16+-0.09), while all [X/Fe] ratios are similar to the solar values. Its elemental abundances are close to those of the Hyades cluster derived from the literature, which seems to reinforce the idea of a possible common origin with the primordial cluster.Comment: 16 pages, 2 figures, 6 tables. Accepted for publication in MNRA

X-Shooter spectroscopy of young stellar objects - VI - HI line decrements

Hydrogen recombination emission lines commonly observed in accreting young stellar objects represent a powerful tracer for the gas conditions in the circumstellar structures. Here we perform a study of the HI decrements and line profiles, from the Balmer and Paschen lines detected in the X-Shooter spectra of a homogeneous sample of 36 T Tauri stars in Lupus, the accretion and stellar properties of which were already derived in a previous work. We aim to obtain information on the gas physical conditions to derive a consistent picture of the HI emission mechanisms in pre-main sequence low-mass stars. We have empirically classified the sources based on their HI line profiles and decrements. We identified four Balmer decrement types (classified as 1, 2, 3, and 4) and three Paschen decrement types (A, B, and C), characterised by different shapes. We first discussed the connection between the decrement types and the source properties and then compared the observed decrements with predictions from recently published local line excitation models. One third of the objects show lines with narrow symmetric profiles, and present similar Balmer and Paschen decrements (straight decrements, types 2 and A). Lines in these sources are consistent with optically thin emission from gas with hydrogen densities of order 10^9 cm^-3 and 5000<T<15000 K. These objects are associated with low mass accretion rates. Type 4 (L-shaped) Balmer and type B Paschen decrements are found in conjunction with very wide line profiles and are characteristic of strong accretors, with optically thick emission from high-density gas (log n_H > 11 cm^-3). Type 1 (curved) Balmer decrements are observed only in three sub-luminous sources viewed edge-on, so we speculate that these are actually reddened type 2 decrements. About 20% of the objects present type 3 Balmer decrements (bumpy), which cannot be reproduced with current models.Comment: 29 pages, accepted by A&

Detection of delta Scuti-like pulsation in H254, a pre-main sequence F-type star in IC 348

We present time series observations of intermediate mass PMS stars belonging to the young star cluster IC 348. The new data reveal that a young member of the cluster, H254, undergoes periodic light variations with delta Scuti-like characteristics. This occurrence provides an unambiguous evidence confirming the prediction that intermediate-mass pre-main sequence (PMS) stars should experience this transient instability during their approach to the main-sequence. On the basis of the measured frequency f=7.406 c/d, we are able to constrain the intrinsic stellar parameters of H254 by means of linear, non adiabatic, radial pulsation models. The range of the resulting luminosity and effective temperature permitted by the models is narrower than the observational values. In particular, the pulsation analysis allows to derive an independent estimate of the distance to IC 348 of about 320 pc. Further observations could either confirm the monoperiodic nature of H254 or reveal the presence of other frequencies.Comment: 7 pages, including 7 postscript figures, accepted for publication on A&

X-shooter spectroscopy of young stellar objects in Lupus: Lithium, iron, and barium elemental abundances

With the purpose of performing a homogeneous determination of elemental abundances for members of the Lupus T association, we analyzed three chemical elements: lithium, iron, and barium. The aims were: to derive the Li abundance for ~90% of known class II stars in the Lupus I, II, III, IV clouds; to perform chemical tagging of a region where few Fe abundance measurements have been obtained in the past, and no determination of the Ba content has been done up to now. We also investigated possible Ba enhancement, as this element has become increasingly interesting in the last years following the evidence of Ba over-abundance in young clusters, the origin of which is still unknown. Using X-shooter@VLT, we analyzed the spectra of 89 cluster members, both class II and III stars. We measured the strength of the Li line and derived the abundance of this element through equivalent width measurements and curves of growth. For six class II stars we also measured the Fe and Ba abundances using the spectral synthesis and the code MOOG. The veiling contribution was taken into account for all three elements. We find a dispersion in the strength of the Li line at low Teff and identify three targets with severe Li depletion. The nuclear age inferred for these highly Li-depleted stars is around 15 Myr, which exceeds the isochronal one. As in other star-forming regions, no metal-rich members are found in Lupus, giving support to a recent hypothesis that the Fe abundance distribution of most of the nearby young regions could be the result of a common and widespread star formation episode involving the Galactic thin disk. We find that Ba is over-abundant by ~0.7 dex with respect to the Sun. Since current theoretical models cannot reproduce this Ba abundance pattern, we investigated whether this unusually large Ba content might be related to effects due to stellar parameters, stellar activity, and accretion.Comment: 15 pages, 14 figures, 3 tables; accepted for publication in A&A; abstract shortene

Glutathione metabolism is essential for self-renewal and chemoresistance of pancreatic cancer stem cells

BACKGROUND Cellular metabolism regulates stemness in health and disease. A reduced redox state is essential for self-renewal of normal and cancer stem cells (CSCs). However, while stem cells rely on glycolysis, different CSCs, including pancreatic CSCs, favor mitochondrial metabolism as their dominant energy-producing pathway. This suggests that powerful antioxidant networks must be in place to detoxify mitochondrial reactive oxygen species (ROS) and maintain stemness in oxidative CSCs. Since glutathione metabolism is critical for normal stem cell function and CSCs from breast, liver and gastric cancer show increased glutathione content, we hypothesized that pancreatic CSCs also rely on this pathway for ROS detoxification. AIM To investigate the role of glutathione metabolism in pancreatic CSCs. METHODS Primary pancreatic cancer cells of patient-derived xenografts (PDXs) were cultured in adherent or CSC-enriching sphere conditions to determine the role of glutathione metabolism in stemness. Real-time polymerase chain reaction (PCR) was used to validate RNAseq results involving glutathione metabolism genes in adherent vs spheres, as well as the expression of pluripotency-related genes following treatment. Public TCGA and GTEx RNAseq data from pancreatic cancer vs normal tissue samples were analyzed using the webserver GEPIA2. The glutathione-sensitive fluorescent probe monochlorobimane was used to determine glutathione content by fluorimetry or flow cytometry. Pharmacological inhibitors of glutathione synthesis and recycling [buthionine-sulfoximine (BSO) and 6-Aminonicotinamide (6-AN), respectively] were used to investigate the impact of glutathione depletion on CSC-enriched cultures. Staining with propidium iodide (cell cycle), Annexin-V (apoptosis) and CD133 (CSC content) were determined by flow cytometry. Self-renewal was assessed by sphere formation assay and response to gemcitabine treatment was used as a readout for chemoresistance. RESULTS Analysis of our previously published RNAseq dataset E-MTAB-3808 revealed up-regulation of genes involved in the KEGG (Kyoto Encyclopedia of Genes and Genomes) Pathway Glutathione Metabolism in CSC-enriched cultures compared to their differentiated counterparts. Consistently, in pancreatic cancer patient samples the expression of most of these up-regulated genes positively correlated with a stemness signature defined by NANOG, KLF4, SOX2 and OCT4 expression (P &lt; 10-5). Moreover, 3 of the upregulated genes (MGST1, GPX8, GCCT) were associated with reduced disease-free survival in patients [Hazard ratio (HR) 2.2-2.5; P = 0.03-0.0054], suggesting a critical role for this pathway in pancreatic cancer progression. CSC-enriched sphere cultures also showed increased expression of different glutathione metabolism-related genes, as well as enhanced glutathione content in its reduced form (GSH). Glutathione depletion with BSO induced cell cycle arrest and apoptosis in spheres, and diminished the expression of stemness genes. Moreover, treatment with either BSO or the glutathione recycling inhibitor 6-AN inhibited self-renewal and the expression of the CSC marker CD133. GSH content in spheres positively correlated with intrinsic resistance to gemcitabine treatment in different PDXs r = 0.96, P = 5.8 × 1011). Additionally, CD133+ cells accumulated GSH in response to gemcitabine, which was abrogated by BSO treatment (P &lt; 0.05). Combined treatment with BSO and gemcitabine-induced apoptosis in CD133+ cells to levels comparable to CD133- cells and significantly diminished self-renewal (P &lt; 0.05), suggesting that chemoresistance of CSCs is partially dependent on GSH metabolism. CONCLUSION Our data suggest that pancreatic CSCs depend on glutathione metabolism. Pharmacological targeting of this pathway showed that high GSH content is essential to maintain CSC functionality in terms of self-renewal and chemoresistance

Accretion-ejection connection in the young brown dwarf candidate ISO-Cha1 217

As the number of observed brown dwarf outflows is growing it is important to investigate how these outflows compare to the well studied jets from young stellar objects. A key point of comparison is the relationship between outflow and accretion activity and in particular the ratio between the mass outflow and accretion rates ($\dot{M}_{out}$/$\dot{M}_{acc}$). The brown dwarf candidate ISO-ChaI 217 was discovered by our group, as part of a spectro-astrometric study of brown dwarfs, to be driving an asymmetric outflow with the blue-shifted lobe having a position angle of $\sim$ 20$^{\circ}$. The aim here is to further investigate the properties of ISO-ChaI 217, the morphology and kinematics of its outflow, and to better constrain ($\dot{M}_{out}$/$\dot{M}_{acc}$). The outflow is spatially resolved in the $[SII]\lambda \lambda 6716,6731$ lines and is detected out to $\sim$ 1\farcs6 in the blue-shifted lobe and ~ 1" in the red-shifted lobe. The asymmetry between the two lobes is confirmed although the velocity asymmetry is less pronounced with respect to our previous study. Using thirteen different accretion tracers we measure log($\dot{M}_{acc}$) [M$_{sun}$/yr]= -10.6 $\pm$ 0.4. As it was not possible to measure the effect of extinction on the ISO-ChaI 217 outflow $\dot{M}_{out}$ was derived for a range of values of A$_{v}$, up to a value of A$_{v}$ = 2.5 mag estimated for the source extinction. The logarithm of the mass outflow ($\dot{M}_{out}$) was estimated in the range -11.7 to -11.1 for both jets combined. Thus $\dot{M}_{out}$/$\dot{M}_{acc}$ [\Msun/yr] lies below the maximum value predicted by magneto-centrifugal jet launching models. Finally, both model fitting of the Balmer decrements and spectro-astrometric analysis of the H$\alpha$ line show that the bulk of the H I emission comes from the accretion flow.Comment: accepted by Astronomy & Astrophysic

Pre-Main Sequence stars in the star forming complex Sh 2-284

Located at the galactic anticenter, Sh 2-284 is a HII region which harbors several young open clusters; Dolidze 25, a rare metal poor (Z~0.004) young cluster, is one of these. Given its association with Sh 2-284, it is reasonable to assume the low metallicity for the whole HII region. Sh~2-284 is expected to host a significant population of Pre-Main Sequence (PMS) stars of both low and intermediate mass stars (Herbig Ae stars). We aim at characterizing these stars by means of a spectroscopic and photometric survey conducted with VIMOS@VLT and complemented with additional optical and infrared observations. In this survey we selected and characterized 23 PMS objects. We derived the effective temperature, the spectral energy distribution and luminosity of these objects; using theoretical PMS evolutionary tracks, with the appropriate metallicity, we estimated the mass and the age of the studied objects. We also estimated a distance of 4 Kpc for Sh 2-284 by using spectroscopic parallax of 3 OB stars. From the age determination we concluded that triggered star formation is in act in this region. Our results show that a significant fraction of the young stellar objects (YSOs) may have preserved their disk/envelopes, in contrast with what is found in other recent studies of low-metallicity star forming regions in the Galaxy. Finally, among the 23 bona fide PMS stars, we identified 8 stars which are good candidates to pulsators of the delta Scuti type.Comment: accepted for publication in MNRA

Hunting for brown dwarf binaries and testing atmospheric models with X-Shooter

The determination of the brown dwarf binary fraction may contribute to the understanding of the substellar formation mechanisms. Unresolved brown dwarf binaries may be revealed through their peculiar spectra or the discrepancy between optical and near-infrared spectral type classification. We obtained medium-resolution spectra of 22 brown dwarfs with these characteristics using the X-Shooter spectrograph at the VLT. We aimed to identify brown dwarf binary candidates, and to test if the BT-Settl 2014 atmospheric models reproduce their observed spectra. To find binaries spanning the L-T boundary, we used spectral indices and compared the spectra of the selected candidates to single spectra and synthetic binary spectra. We used synthetic binary spectra with components of same spectral type to determine as well the sensitivity of the method to this class of binaries. We identified three candidates to be combination of L plus T brown dwarfs. We are not able to identify binaries with components of similar spectral type. In our sample, we measured minimum binary fraction of $9.1^{+9.9}_{-3.0}$. From the best fit of the BT-Settl models 2014 to the observed spectra, we derived the atmospheric parameters for the single objects. The BT-Settl models were able to reproduce the majority of the SEDs from our objects, and the variation of the equivalent width of the RbI (794.8 nm) and CsI (852.0 nm) lines with the spectral type. Nonetheless, these models did not reproduce the evolution of the equivalent widths of the NaI (818.3 nm and 819.5 nm) and KI (1253 nm) lines with the spectral type.Comment: Accepted for publication in MNRA

Estructura poblacional del Podenco Andaluz

En nuestro país los libros genealógicos caninos están gestionados por la Real Sociedad de Razas Caninas en España, quien delega su mantenimiento y control en las distintas asociaciones de criadores afiliadas. En el caso de la raza Podenco Andaluz, la gestión del libro genealógico es especialmente compleja debido al aislamiento reproductivo entre las distintas variedades existentes que obliga el actual patrón racial. En el presente estudio analizamos la estructura genética de la población del Podenco Andaluz basándonos en los registros genealógicos de los cinco últimos años donde indagamos en los ratios por sexo, ratios por tallas, ratios por edades dentro de talla; su evolución durante este tiempo y su proyección el futuro. Asimismo, profundizamos en una análisis de los niveles de consanguinidad individuales de los ejemplares inscritos, así como del nivel de endogamia media de la población