Assessment of Microbial Quality and Antibacterial Activity of Commonly used Hand Washes

Hands are the highways to the transmission and spread of pathogens that causes diseases, food borne illnesses and nosocomial infections. Hand washing is the act of cleansing the hands with water or another liquid, with or without the use of soap or other detergents, to ensure proper hand hygiene. To determine the microbiological quality and the antibacterial property and dilution effects on activity of hand wash, seven brands of hand washes were evaluated using susceptibility test by agar well diffusion, minimum inhibitory dilution and time kill test. This was done by assessing different dilutions of the hand washes against standardized 1.5x108 cells of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Results showed that all the hand washes were sterile and all the brands had some level of antibacterial activity. The hand washes were more active on Staphylococcus aureus and Escherichia coli than on Pseudomonas aeruginosa. Activity decreased with dilution as neat and 2-1 dilutions gave better results compared to 2-2, 10-1 and 10-2. Hib hand wash killed all test organisms when exposed for 2, 5 and 10 minutes and at neat and 2-1 dilutions but not at 2-2, 10-1 and 10-2. Rev and Pan hand washes though are sterile, were least active in all the tests. The minimum inhibitory dilution and minimum bactericidal dilution for most of the hand wash was at neat concentration. The time kill test showed that the effect of the hand wash was highest at 5 and 10 minutes and at neat (undiluted) for all the test organisms. It is advised that the dilution of hand washes a common practice in most eateries must stop as these products are not active when diluted, hands should be washed for five to ten minutes for maximum hand hygiene. Key words: Antibacterial Activity, Food Borne Illnesses, Hand Hygiene, Microbiological Quality, Nosocomial Infections and Pathogens

Exchange parameters from approximate self-interaction correction scheme

The approximate atomic self-interaction corrections (ASIC) method to density functional theory is put to the test by calculating the exchange interaction for a number of prototypical materials, critical to local exchange and correlation functionals. ASIC total energy calculations are mapped onto an Heisenberg pair-wise interaction and the exchange constants J are compared to those obtained with other methods. In general the ASIC scheme drastically improves the bandstructure, which for almost all the cases investigated resemble closely available photo-emission data. In contrast the results for the exchange parameters are less satisfactory. Although ASIC performs reasonably well for systems where the magnetism originates from half-filled bands, it suffers from similar problems than those of LDA for other situations. In particular the exchange constants are still overestimated. This reflects a subtle interplay between exchange and correlation energy, not captured by the ASIC.Comment: 10 page

The prevalence of hypocalcemia in pregnancy and peurperium in northwestern Nigeria.

Background: The occurrence of hypocalcaemia in pregnancy and puerperium is not often reported in literature even though calcium supplementation is not routine in Nigeria. A finding of a relatively increased number of cases seen with hypocalcaemia in pregnancy and following childbirth necessitated a review of records to find risk factors and other associations.Objectives: To evaluate the prevalence of hypocalcaemia and identify risk factors for its occurrence among women presenting with carpopedal spasm in pregnancy and puerperium in our area of practice.Study Design Setting And Subjects: This is a five year retrospective study involving the retrieval of patient records presenting with carpopedal spasms from 1st June 2010 to 30th June 2015 at Rasheed Shekoni specialist hospital, Duste Jigawa State NigeriaMethod: The total number of deliveries within review period as well as the case records of women presenting with features of hypocalcaemia were retrieved manually and relevant data extracted including demographics, parity and duration of symptoms as well as laboratory indices. Analysis was done using relevant statistical software.Result: There were a total of 3989 deliveries with 52 presenting with carpopedal spasm giving a prevalence of 1.3%. The average age was 26.6 years ± 6.6 years and parity of 4.1±2.9. The mean serum calcium level was 1.72mmol/l ± 0.32 mmol/l. There was a negative correlation between parity and serum calcium levels (ρ= - 0.296, p=0.151)Conclusion: The prevalence of hypocalcaemia at 1.3% was higher than earlier reports elsewhere. High parity was found to be a likely risk factor. We suggest that calcium supplementation should be considered for these groups of women who in addition have other risk factors in the antenatal period.Keywords: Parathormone, 1, 25-dihydroxyvitamin D, Hypocalcemia, Parit

Chemical constituents, toxicity and antimicrobial activities of the essential oil of the leaves of Tectona grandis

The leaves of Tectona grandis (Verbanaceae) was subjected to hydrodistillation in order to obtain the essential oil from the plant. The pale yellow essential oil gave a percentage yield of 0.184%. Relative percentages of individual component were analyzed by GC/GC-MS. A total of fifty-four (54) constituents were identified representing 86.5% of the total essential oil fraction. Oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, apocarotenoids, phenylpropanoids and non-terpene derivatives were the various classes of compounds identified. The LC50 value from the brine shrimp toxicity assay was 183.29µg/ml. The oil extract was also subjected to antibacterial assay and it showed significant activities against all the clinical test organisms used except Pseudomonas aeruginos

Electric field response of strongly correlated one-dimensional metals: a Bethe-Ansatz density functional theory study

We present a theoretical study on the response properties to an external electric field of strongly correlated one-dimensional metals. Our investigation is based on the recently developed Bethe-Ansatz local density approximation (BALDA) to the density functional theory formulation of the Hubbard model. This is capable of describing both Luttinger liquid and Mott-insulator correlations. The BALDA calculated values for the static linear polarizability are compared with those obtained by numerically accurate methods, such as exact (Lanczos) diagonalization and the density matrix renormalization group, over a broad range of parameters. In general BALDA linear polarizabilities are in good agreement with the exact results. The response of the exact exchange and correlation potential is found to point in the same direction of the perturbing potential. This is well reproduced by the BALDA approach, although the fine details depend on the specific parameterization for the local approximation. Finally we provide a numerical proof for the non-locality of the exact exchange and correlation functional.Comment: 8 pages and 8 figure

Treatment of malaria in pregnancy: Knowledge of community pharmacists in Ibadan, Nigeria

Purpose: To evaluate the knowledge of community pharmacists in the management of malaria in pregnancy and their adherence to the World Health Organisation (WHO) treatment guidelines. Methods: Questionnaires were administered to obtain information on knowledge and adherence to WHO guidelines. Respondents’ scores on a 12-item knowledge question on the management of malaria in pregnancy were categorized as good knowledge if ≥ 10, and poor knowledge if < 10. Descriptive statistics were used to summarise the data. Chi-square test was used to explore the association between sociodemographic characteristics and knowledge. Results: Pharmacists qualified within 10 years of the study period were 29 (48.3 %). Only 25 (31.3 %) of the pharmacists possessed an additional qualification to Bachelor of Pharmacy degree. Rapid diagnostic test kits (RDT) and light microscopy test (LMT) have been used by 51 (76.2 %) and 17 (28.8 %), respectively, to diagnose malaria. About three-quarters (71.3 %) had poor knowledge of the medication for the treatment of malaria in pregnancy. Knowledge score was neither significantly associated with the pharmacists’ years of qualification (p = 0.174) nor with possession of additional qualification (p = 0.334). Conclusions: There is a substantial gap in pharmacists’ knowledge on the management of malaria in pregnancy, revealing an urgent need for training and adherence of community pharmacists to World Health Organisation treatment guidelines to ensure the safety of pregnant women and the unborn baby

Assessment of Heavy Metals Level in Soil and Vegetables Grown in Peri-Urban Farms around Osun State and the Associated Human Health Risk

Farming around urban centres (peri-urban farming) is a major source of fresh crop produce, notably vegetables. However, the limitation of land resources and the associated high level of soil contamination from domestic and industrial pollutants are major concerns for the safety of food materials from peri-urban farms. Thus, this study investigated heavy metals (As, Cd, Cu, Pb and Zn) concentration in soil and vegetable samples (Amaranthus hybridus and Corchorus olitorius) collected from selected peri-urban farms with a view to providing information on the human health risks associated with consumption of peri-urban vegetables.This study showed that the concentration of investigated heavy metals in the soils of peri-urban farms were within the background range for farming set by FAO/WHO (2002) and EU (2006) while appreciable level of these metals were observed in vegetable samples. Arsenic concentration was below detection limit in all samples. Amaranthus showed higher retention capacity for the assayed heavy metals except Cu. Transfer Factor values showed metal uptake by vegetables in the order Cd > Zn > Pb > Cu. The estimated daily intake showed that the highest consumption of Cd, Cu, Pb and Zn were from Amaranthus. The Health risk index showed high values for Cd and Pb but low values for Cu and Zn for both Amaranthus and Corchorus. The results obtained in this study regarding the hazard index indicate that vegetables grown in selected peri-urban farms are not safe for consumption

Effect of drinking magnetized water on immunity and carcass quality of broiler chicken

This study was conducted to determine the effect of drinking Magnetized Water (MW) on the immunity of broiler chicken. The problem which this study focused on was the mortality rate of broiler chicken which is common in some poultry farms in Nigeria due to some factors that could lead to loss of income. There is a need for economical methods for boosting the immunity of Broiler Chicken (BC) in order to reduce the mortality rate. BC (Arbor Acres breed) was given MW. The magnetized water was produced by passing water through the magnetic field in a pipe. The treatments were MW treated for 33s by passing the water through pipe surrounded with magnets one time(T1), MW treated for 66 s by flowing twice through the magnetic unit (T2), T3 was MW treated for 99 s and control (Non-Magnetized Water, NMW, T0). A total of 80 BCs (day old) with 20 BC for each treatment were given MW (T1, T2 and T3), NMW and monitored for 7 weeks. Blood samples were collected for hematology and analyzed using standard methods. The values of white blood cell for T0, T1, T2 and T3 were 153.61×10-9/L, 133.20×10-9/L, 134.78×10-9/L and 101.12×10-9/L and for lymphocytes, were 137.08×10-9/L, 120.79×10-9/L, 125.49×10-9/L and 96.12×10-9/L, respectively. MW T1, T2 and T3 increased body weight gain of the BC by 410, 320 and 210g/broiler. Protein contents of the carcass for T1, T2, T3 and T0 were 13.13%, 13.23%, 13.18% and 12.80%, respectively. MW can therefore enhance production of healthy broiler chickens