Inhibition of phosphoinositide 3-kinase/protein kinase B signaling hampers the vasopressin-dependent stimulation of myogenic differentiation

Arginine-vasopressin (AVP) promotes muscle differentiation, hypertrophy, and regeneration through the combined activation of the calcineurin and Calcium/Calmodulin-dependent Protein Kinase (CaMK) pathways. The AVP system is impaired in several neuromuscular diseases, suggesting that AVP may act as a physiological factor in skeletal muscle. Since the Phosphoinositide 3-kinases/Protein Kinase B/mammalian Target Of Rapamycin (PI3K/Akt/mTOR) signaling plays a significant role in regulating muscle mass, we evaluated its role in the AVP myogenic effect. In L6 cells AKT1 expression was knocked down, and the AVP-dependent expression of mTOR and Forkhead box O3 (FoxO) was analyzed by Western blotting. The effect of the PI3K inhibitor LY294002 was evaluated by cellular and molecular techniques. Akt knockdown hampered the AVP-dependent mTOR expression while increased the levels of FoxO transcription factor. LY294002 treatment inhibited the AVP-dependent expression of Myocyte Enhancer Factor-2 (MEF2) and myogenin and prevented the nuclear translocation of MEF2. LY294002 also repressed the AVP-dependent nuclear export of histone deacetylase 4 (HDAC4) interfering with the formation of multifactorial complexes on the myogenin promoter. We demonstrate that the PI3K/Akt pathway is essential for the full myogenic effect of AVP and that, by targeting this pathway, one may highlight novel strategies to counteract muscle wasting in aging or neuromuscular disorders

The Real Meaning of Complex Minkowski-Space World-Lines

In connection with the study of shear-free null geodesics in Minkowski space, we investigate the real geometric effects in real Minkowski space that are induced by and associated with complex world-lines in complex Minkowski space. It was already known, in a formal manner, that complex analytic curves in complex Minkowski space induce shear-free null geodesic congruences. Here we look at the direct geometric connections of the complex line and the real structures. Among other items, we show, in particular, how a complex world-line projects into the real Minkowski space in the form of a real shear-free null geodesic congruence.Comment: 16 page

Major impact from a minor merger - The extraordinary hot molecular gas flow in the Eye of the NGC 4194 Medusa galaxy

Minor mergers are important processes contributing significantly to how galaxies evolve across the age of the Universe. Their impact on supermassive black hole growth and star formation is profound. The detailed study of dense molecular gas in galaxies provides an important test of the validity of the relation between star formation rate and HCN luminosity on different galactic scales. We use observations of HCN, HCO+1-0 and CO3-2 to study the dense gas properties in the Medusa merger. We calculate the brightness temperature ratios and use them in conjunction with a non-LTE radiative line transfer model. The HCN and HCO+1-0, and CO3-2 emission do not occupy the same structures as the less dense gas associated with the lower-J CO emission. The only emission from dense gas is detected in a 200pc region within the "Eye of the Medusa". No HCN or HCO+ is detected for the extended starburst. The CO3-2/2-1 brightness temperature ratio inside "the Eye" is ~2.5 - the highest ratio found so far. The line ratios reveal an extreme, fragmented molecular cloud population inside "the Eye" with large temperatures (>300K) and high gas densities (>10^4 cm^-3). "The Eye" is found at an interface between a large-scale minor axis inflow and the Medusa central region. The extreme conditions inside "the Eye" may be the result of the radiative and mechanical feedback from a deeply embedded, young, massive super star cluster, formed due to the gas pile-up at the intersection. Alternatively, shocks from the inflowing gas may be strong enough to shock and fragment the gas. For both scenarios, however, it appears that the HCN and HCO+ dense gas tracers are not probing star formation, but instead a post-starburst and/or shocked ISM that is too hot and fragmented to form new stars. Thus, caution is advised in linking the detection of emission from dense gas tracers to evidence of ongoing or imminent star formation.Comment: 10 pages, 5 figures, 2 tables, accepted for publication in A&

Characterizing spiral arm and interarm star formation

Interarm star formation contributes significantly to a galaxy's star formation budget, and provides an opportunity to study stellar birthplaces unperturbed by spiral arm dynamics. Using optical integral field spectroscopy of the nearby galaxy NGC 628 with VLT/MUSE, we construct Halpha maps including detailed corrections for dust extinction and stellar absorption to identify 391 HII regions at 35pc resolution over 12 kpc^2. Using tracers sensitive to the underlying gravitational potential, we associate HII regions with either arm (271) or interarm (120) environments. Using our full spectral coverage of each region, we find that most HII region physical properties (luminosity, size, metallicity, ionization parameter) are independent of environment. We calculate the fraction of Halpha luminosity due to the diffuse ionized gas (DIG) background contaminating each HII region, and find the DIG surface brightness to be higher within HII regions compared to the surroundings, and slightly higher within arm HII regions. Use of the temperature sensitive [SII]/Halpha line ratio map instead of the Halpha surface brightness to identify HII region boundaries does not change this result. Using the dust attenuation as a tracer of the gas, we find depletion times consistent with previous work (2 x 10^9 yr) with no differences between the arm and interarm, however this is very sensitive to the DIG correction. Unlike molecular clouds, which can be dynamically affected by the galactic environment, we see fairly consistent HII region properties in both arm and interarm environments. This suggests either a difference in arm star formation and feedback, or a decoupling of dense star forming clumps from the more extended surrounding molecular gas.Comment: 10 pages, 4 figures, 1 table, accepted for publication in Ap

Sustainability Determinants of Cultural and Creative Industries in Peripheral Areas.

Cultural and Creative Industries (CCIs) are increasingly recognized as part of the global economy and of growing importance for sustainable local development. However, the exploitation of their full potential depends on several issues concerning their entrepreneurial dimension and the context where they operate. The paper deals with these issues having the scope to investigate the main determinants of CCIs’ sustainability in peripheral areas, to understand what kind of policy could better support the survival of CCIs and development in these areas, according to an end-user perspective. The research is part of an Interreg Greece-Italy project carried out from mid-2018 until the end of 2020 with specific reference to CCIs in Apulia (IT) andWestern Greece (EL). A two-step mixed methodology has been used to figure out regional specializations and the specific aspects of the entrepreneurial structure and business sustainability in the cultural and creative sector (CCs). In the end, the paper shows and discusses the main determinants considered crucial for CCI sustainability, suggesting guidelines for local authorities supporting their economic development

The Snapshot Hubble U-Band Cluster Survey (SHUCS) II. Star Cluster Population of NGC 2997

We study the star cluster population of NGC 2997, a giant spiral galaxy located at 9.5 Mpc and targeted by the Snapshot Hubble U-band Cluster Survey (SHUCS). Combining our U-band imaging from SHUCS with archival BVI imaging from HST, we select a high confidence sample of clusters in the circumnuclear ring and disk through a combination of automatic detection procedures and visual inspection. The cluster luminosity functions in all four filters can be approximated by power-laws with indices of $-1.7$ to $-2.3$. Some deviations from pure power-law shape are observed, hinting at the presence of a high-mass truncation in the cluster mass function. However, upon inspection of the cluster mass function, we find it is consistent with a pure power-law of index $-2.2\pm0.2$ despite a slight bend at $\sim$$2.5\times10^{4}$ M$_{\odot}$. No statistically significant truncation is observed. From the cluster age distributions, we find a low rate of disruption ($\zeta\sim-0.1$) in both the disk and circumnuclear ring. Finally, we estimate the cluster formation efficiency ($\Gamma$) over the last 100 Myr in each region, finding $7\pm2$% for the disk, $12\pm4$% for the circumnuclear ring, and $10\pm3$% for the entire UBVI footprint. This study highlights the need for wide-field UBVI coverage of galaxies to study cluster populations in detail, though a small sample of clusters can provide significant insight into the characteristics of the population.Comment: 31 pages, 9 figures, accepted to the A

Comparison of MODIS and ETA profiles of atmospheric parameters in coastal zones with radiosonde data

The quality of atmospheric profiles gathered by the spaceborne Moderate Resolution Imaging Spectroradiometer (MODIS) sensor onboard the Terra platform and those predicted by the ETA atmospheric circulation model are assessed against corresponding radiosonde (RS)measurements. The analysis is carried out on a statistical basis taking as reference the radiosoundings collected at two coastal stations, namely Ajaccio (France) and Pratica di Mare (Italy), during the spring 2000. The examined days were characterized by smooth and slow variations of the atmospheric conditions so that a temporal lag up to about three hours between RS and MODIS profiles could be considered for comparison purposes. Both ETA predictions and MODIS retrievals compare well with RS data and their relative agreement is good. Although, as expected, the profiles of the analyzed quantities, namely temperature and moisture for both MODIS and ETA outputs and horizontal wind components predicted by ETA model, could not follow the largest fluctuations measured by RS, their averages are reproduced with a satisfactory degree of reliability. These results encourage the perspective to exploit remote measurements from the MODIS sensor of atmospheric temperature and water vapour as input to operative circulation models, such as ETA, for reliable forecasts and detailed monitoring on global scale of the atmospheric structure and dynamics