The role of hypoxia-inducible factor post-translational modifications in regulating its localisation, stability, and activity

Abstract The hypoxia signalling pathway enables adaptation of cells to decreased oxygen availability. When oxygen becomes limiting, the central transcription factors of the pathway, hypoxia-inducible factors (HIFs), are stabilised and activated to induce the expression of hypoxia-regulated genes, thereby maintaining cellular homeostasis. Whilst hydroxylation has been thoroughly described as the major and canonical modification of the HIF-α subunits, regulating both HIF stability and activity, a range of other post-translational modifications decorating the entire protein play also a crucial role in altering HIF localisation, stability, and activity. These modifications, their conservation throughout evolution, and their effects on HIF-dependent signalling are discussed in this review

RelA-DsRedxp E2F1-Venus BAC stables Representitive cell traces

RelA-DsRedexpress and E2F1-Venus BAC stables Representative cell traces separated into cell cycle phases by virtual synchronization

RelA-DsRedxp E2F1-Venus BAC stables FCS Autocorrolation and cross-corrolation data

RelA-DsRedxp E2F1-Venus BAC stables FCS autocorrolation and cross-corrolation dat

HeLa FUCCI ANALYSIS

Analysis of cell cycle transition from G1 to S using Fucci vectors in HeLa cells as well as analysis of mean Mitosis length in the cell typ

RelA-DsRedxp E2F1-Venus BAC stables FCS molecular fluoresence Data

Molecular fluorescence counts in single cells of RelA-DsRedxp E2F1-Venus BAC stables and correlation data

Effect of TNFalpha on HeLa and SK-N-AS cell cycle length

Cell cycle lengths for SK-N-AS and HeLa cells without and with TNFalpha stimulation. Effect extension on cell cycle following stimulation is also shown at G1 and S phases

Peak RelA Nuclear Amplitude for HeLa Cells

Peak RelA Nuclear Amplitude for HeLa Cells following TNF stimulation arranged by virtual synchronization of cell cycle. Single cell traces upon which the peak amplitude data is based are also include

120210 AS FUCCI O+G CTRL ANALYSIS

Analysis of cell cycle transition from G1 to S using Fucci vectors in SK-N-AS cells as well as analysis of mean Mitosis length in the cell typ

BAC BAC Stable cell phase Meta data_Dryad

Traces of BAC-BAC E2F-1-Venus and Rel-A-dsRedexpress sorted into different cell cycle phases