Restoration possibilities of dry grasslands afforested by pine: the role of seed bank and remnant vegetation

Stipa-dominated steppe-like dry grasslands represent a great nature conservation priority in Romania and in the whole European Union. In Romania, due to their low productivity, many sites had been afforested by pines or black locust trees at the end of the 1950’s in order to raise economic value and to hinder landslides on the steep slopes. Economic expectations did not live up, thus afforested sites will increasingly be targeted for grassland restoration, especially in protected areas of the Natura 2000 network. We analysed seed bank and aboveground vegetation in a 40-year-old black pine plantation site established on a former dry grassland near Viișoara (Transylvanian Lowland), and compared to that of an adjacent intact dry grassland stand. In both stands, eight 5 m × 5 m plots for soil and vegetation analysis were established. Inside each plot, six cylinder-shaped soil cores of 4 cm diameter and 10 cm depths were drilled; soil samples from the same plot were subsequently mixed up. Soil sampling was carried out in early spring, 2008. We analysed seed densities and composition in soil samples using the seedling emergence method. In addition, we recorded percentage cover of plant species in the plots in June. We wanted to find out on what extent we can rely on the soil seed bank and remnant vegetation, if active interventions are planned to restore a grassland stand in the place of a pine plantation. In total, 293 seedlings belonging to 51 species have emerged from soil samples. Seed density in the soil seed bank of the pine plantation was substantially higher (3500 ± 2059 seeds m-2, 0–10 cm) than that of the intact grassland (1360 ± 469 seeds m-2, 0–10 cm). Our results showed that the seed bank of the plantation site, although not specifically species rich, may still contain a considerable amount of target species. In addition, we found strong populations of grassland species in the understory of the plantation as remnants of the former vegetation, which, together with seeds in the seed bank can ensure a good starting point to a successful natural regeneration after tree removal. Due to the presence of ruderal species in the seed bank and shrubs in the aboveground vegetation, light restoration measures comprising weed- and shrub control are necessary following the complete removal of trees. We propose the re-introduction of grazing in order to ensure the spontaneous regeneration by propagule transport to restoration sites

Allometric relationships between diaspore morphology and diaspore covering anatomy of herbaceous species from central-eastern Europe

AbstractAnatomical and morphological seed traits are of great ecological importance and are a main subject of, for example, seed bank or endozoochory studies. However, we observed a lack of information about the relationship between seed anatomy and seed morphology and its ecological implications. To fill this gap, we linked the anatomical features of diaspore coverings to morphological characteristics of free seeds and one-seeded fruits. We predicted that: (1) the thickness and anatomical complexity of seed coat and pericarp are related to diaspore size and shape; and (2) the presence or absence of the pericarp may influence seed-coat thickness and anatomy. In our study we investigated diaspores of 39 central-eastern European herbaceous species and recorded the thickness and anatomical complexity of their seed coverings, and we determined diaspore mass and shape. Our results indicate that diaspore mass is positively related to covering thickness, lignification degree and anatomical complexity. This might be the case because bigger diaspores tend to remain on the soil surface and are more exposed to predation risk and environmental threat than smaller diaspores. Furthermore, more round-shaped diaspores had disproportionately thicker and more lignified coverings than long or flat ones, probably because round-shaped diaspores much more frequently form seed banks and therefore persist for a long time in the soil. We also found that free seeds as diaspores have a thicker and more lignified seed coat than seeds enclosed in fruits. In one-seeded fruits, the pericarp ‘takes the protective role’, it is thick, and the seed coat is poorly developed.</jats:p

Differential effects of several “litter” types on the germination of dry grassland species

Abstract Question: Accumulation of litter can have serious implications on the recruitment of plant species, by modifying the physical, biological and chemical features of the microenvironment or acting as a mechanical barrier for seeds and seedlings. Isolating these different effects has rarely been achieved experimentally. Location: Transylvanian Lowland, Romania. Methods: We tested the effects of different &apos;&apos;litter&apos;&apos; types on the germination of dry grassland species using a controlled pot experiment with three natural litter types, differing in decay state and composition (Stipa pulcherrima fresh leaves, partly decomposed leaves and mixed and partly decomposed plant material) and an artificial plastic litter, with two levels of water addition. As a complementary field study, seed sowing was conducted in grassland plots with litter removal and plastic litter application. Results: Litter effects were mainly positive (intermittent watering) or neutral (frequent watering) under controlled experimental conditions, and mostly negative in the field. Seed size and environmental conditions were the major determinants of litter effects on germination. Significant differences were found in the effect of litter type on germination, much of which could be explained by chemical factors determined by the decay state, as we confirmed a higher concentration of allelopathic compounds in fresh S. pulcherrima litter than in the senescing leaf litter. Conclusions: The effects of litter on seed germination are strongly context dependent, and it is hard to define common rules that apply consistently under various environmental conditions. &apos;&apos;Litter&apos;&apos; identity and quality matter, i.e. the litter composition and decay state, and influence seed germination

Explanation of beta diversity in European alpine grasslands changes with scale

The importance of environmental difference among sites and dispersal limitations of species to the explanation of diversity differs among biological systems and geographical regions. We hypothesized that climate and then dispersal limitation will predominantly explain the similarity of alpine vegetation at increasing distances between pairs of regions at subcontinental extent. We computed the similarity of all pairs of 23 European mountain regions below 50 degrees N after dividing the species lists of each region by calcareous or siliceous substrates. Distance decay in similarity was better fitted by a cubic polynomial than a negative exponential function, and the fit was better on calcareous than on siliceous substrate. Commonality analysis revealed that the proportion of explanation of beta diversity by climatic difference had unimodal patterns on a gradient of increasing distance between regions, while explanation by dispersal limitation had consistently rising patterns on both substrates. On siliceous substrate, dispersal limitation explained more of the variation in beta diversity only at longer distances, but it was predominant at all distances on calcareous substrate. The steeper response to distance at 2600 km may indicate dispersal limitation at different temporal scales, and the uptick in the response to distance at the longest distances may reflect how isolated some regions have been before and since the last glacial maximum

Long-term land-cover/use change in a traditional farming landscape in Romania inferred from pollen data, historical maps and satellite images

Traditional farming landscapes in the temperate zone that have persisted for millennia can be exceptionally species-rich and are therefore key conservation targets. In contrast to Europe’s West, Eastern Europe harbours widespread traditional farming landscapes, but drastic socio-economic and political changes in the twentieth century are likely to have impacted these landscapes profoundly. We reconstructed long-term land-use/cover and biodiversity changes over the last 150 years in a traditional farming landscape of outstanding species diversity in Transylvania. We used the Regional Estimates of Vegetation Abundance from Large Sites model applied to a pollen record from the Transylvanian Plain and a suite of historical and satellite-based maps. We documented widespread changes in the extent and location of grassland and cropland, a loss of wood pastures as well as a gradual increase in forest extent. Land management in the socialist period (1947–1989) led to grassland expansion, but grassland diversity decreased due to intensive production. Land-use intensity has declined since the collapse of socialism in 1989, resulting in widespread cropland abandonment and conversion to grassland. However, these trends may be temporary due to both ongoing woody encroachment as well as grassland management intensification in productive areas. Remarkably, only 8% of all grasslands existed throughout the entire time period (1860–2010), highlighting the importance of land-use history when identifying target areas for conservation, given that old-growth grasslands are most valuable in terms of biodiversity. Combining datasets from different disciplines can yield important additional insights into dynamic landscape and biodiversity changes, informing conservation actions to maintain these species-rich landscapes in the longer term

Mapping species richness of plant families in European vegetation

Aims: Biodiversity is traditionally studied mostly at the species level, but biogeographical and macroecological studies at higher taxonomic levels can provide valuable insights into the evolutionary processes at large spatial scales. Our aim was to assess the representation of vascular plant families within different vegetation formations across Europe. Location: Europe. Methods: We used a data set of 816,005 vegetation plots from the European Vegetation Archive (EVA). For each plot, we calculated the relative species richness of each plant family as the number of species belonging to that family divided by the total number of species. We mapped the relative species richness, averaged across all plots in 50 km × 50 km grid cells, for each family and broad habitat groups: forests, grasslands, scrub and wetlands. We also calculated the absolute species richness and the Shannon diversity index for each family. Results: We produced 522 maps of mean relative species richness for a total of 152 vascular plant families occurring in forests, grasslands, scrub and wetlands. We found distinct spatial patterns for many combinations of families and habitat groups. The resulting series of 522 maps is freely available, both as images and GIS layers. Conclusions: The distinct spatial patterns revealed in the maps suggest that the relative species richness of plant families at the community level reflects the evolutionary history of individual families. We believe that the maps and associated data can inspire further biogeographical and macroecological studies and strengthen the ongoing integration of phylogenetic, functional and taxonomic diversity concepts.MV, IA, JPC, ZL, IK, AJ and MC were funded by the Czech Science Foundation, programme EXPRO (project no. 19-28491X); JDi by the Czech Science Foundation (18-02773S); IB and JAC by the Basque Government (IT936-16); AČ by the Slovenian Research Agency (ARRS, P1-0236); AK by the National Research Foundation of Ukraine (project no. 2020.01/0140); JŠ by the Slovak Research and Development Agency (APVV 16-0431); KV by the National Science Fund (Contract DCOST 01/7/19.10.2018)

Post-glacial determinants of regional species pools in alpine grasslands

[Aim] Alpine habitats support unique biodiversity confined to high-elevation areas in the current interglacial. Plant diversity in these habitats may respond to area, environment, connectivity and isolation, yet these factors have been rarely evaluated in concert. Here we investigate major determinants of regional species pools in alpine grasslands, and the responses of their constituent species groups.[Location] European mountains below 50° N.[Time period] Between 1928 and 2019.[Major taxa studied] Vascular plants.[Methods] We compiled species pools from alpine grasslands in 23 regions, including 794 alpine species and 2,094 non-alpines. We used species–area relationships to test the influence of the extent of alpine areas on regional richness, and mixed-effects models to compare the effects of 12 spatial and environmental predictors. Variation in species composition was addressed by generalized dissimilarity models and by a coefficient of dispersal direction to assess historical links among regions.[Results] Pool sizes were partially explained by current alpine areas, but the other predictors largely contributed to regional differences. The number of alpine species was influenced by area, calcareous bedrock, topographic heterogeneity and regional isolation, while non-alpines responded better to connectivity and climate. Regional dissimilarity of alpine species was explained by isolation and precipitation, but non-alpines only responded to isolation. Past dispersal routes were correlated with latitude, with alpine species showing stronger connections among regions.[Main conclusions] Besides area effects, edaphic, topographic and spatio-temporal determinants are important to understand the organization of regional species pools in alpine habitats. The number of alpine species is especially linked to refugia and isolation, but their composition is explained by past dispersal and post-glacial environmental filtering, while non-alpines are generally influenced by regional floras. New research on the dynamics of alpine biodiversity should contextualize the determinants of regional species pools and the responses of species with different ecological profiles.The authors thank Daniela Gaspar for support in GIS analyses. B.J.-A. thanks the Marie Curie Clarín-COFUND program of the Principality of Asturias-EU (ACB17-26), the regional grant IDI/2018/000151, and the Spanish Research Agency grant AEI/ 10.13039/501100011033. J.V.R.-D. was supported by the ACA17-02FP7 Marie Curie COFUND-Clarín grant. G.P.M. was funded by US National Science Foundation award 1853665. C.M. was funded by grant no. 19-28491 of the Czech Science Foundation.Peer reviewe