1,572 research outputs found
Stability, conformational plasticity, oligomerization behaviour and equilibrium unfolding intermediates of the Ebola virus matrix protein VP40
Análisis y Metodología de Ensayo de Confort y Condiciones de Carga de Material Móvil Ferroviario Remolcado
El objetivo de este estudio es realizar un análisis de las condiciones de carga y el confort del material móvil ferroviario remolcado y, posteriormente, desarrollar una metodología de ensayo válida. Se han estudiado, por un lado, las condiciones de carga a las que está sometido un bogie de un vehículo ferroviario y, por otro, el confort para un vehículo ferroviario equipado con el bogie en cuestión. Para ello se ha realizado un análisis de los requisitos de la normativa relacionada (sobre todo las UIC 515-4 y UIC 518), una especificación del protocolo de ensayo a seguir y una ejecución y análisis de los resultados obtenidos. Esta metodología de estudio ha dado muy buen resultado a la hora de determinar, por un lado, los esfuerzos sufridos por un bogie en diferentes condiciones de carga y, por otro, las condiciones de confort de un material móvil ferroviario remolcado tanto en tara como en condiciones de carga máxima, en una circulación normal y variando su velocidad. Las herramientas software desarrolladas son muy flexibles y permiten su extensión a cualquier tipo de estudio de este tipo
First steps towards the design of an enzymatic optical (nano)biosensor for the determination of glucose.
This study describes the first steps towards the design of an enzymatic optical (nano)biosensor for the determination of glucose. The aim of this work is to relate the optical properties of gold nanomaterials with glucose concentration. In order to achieve this goal, the redox properties of FAD cofactor of the glucose oxidase enzyme were used. During the enzymatic reaction between glucose oxidase and glucose, the FAD cofactor is reduced to FADH2. A gold precursor in oxidation state 3+ is used to reoxidize the enzyme which at the same time is reduced to gold in oxidation state 0. Gold in oxidation state 0 can be developed into two different types of nanomaterials: gold nanoparticles (AuNPs) or gold nanoclusters (AuNCs). These nanomaterials have different optical properties which could be related to the glucose concentration. On the one hand AuNPs exhibit absorption properties due to surface plasmon resonance (SPR). On the other hand AuNCs are composed by only few atoms, its size is around 2 nm or less and displays fluorescence. The intensity of SPR of gold nanoparticles has been successfully related to the glucose concentration. In addition, kinetics studies have shown a relationship between the velocity at which the fluorescent intensity of AuNCs increases and the glucose concentration
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Session D7: The Most Evaluated Fishway in Spain: A New Lesson Every Year
Abstract:
Salto de San Fernando” hydropower plant is placed at River Tormes, just upstream of Santa Teresa reservoir (Salamanca, Spain). The dam is 13 m high and it has a pool and weir with bottom orifice fish ladder to let potamodromous fish (Iberian barbel – Luciobarbus bocagei–, Nothern straightmouth nase –Pseudochondrostoma duriense– and brown trout –Salmo trutta–) overcome the obstacle. This fish ladder is being monitored since 2012: daily trapping and counting fish that reach the last pool; fish passage video recording through orifices and spillways; tagging fish (PIT, Tbar and others) and studying passage metrics (location, entrance, passage time, performance). Results have been analyzed as a function of physical –discharge, temperature, atmospheric pressure– and biological variables –species, sex, size, competition–. Downstream migration is also analyzed to locate fish routes and understand fish behavior. After every research, passage improvement options are detected, accomplished and assessed on next migration season. At this moment, this overall approach has quintupled fish ladder efficiency and it offers lot of information about Iberian fish behavior and their preference under different hydraulic conditions
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Session B9: Influence of Biometric Parameters, Flow Condition and Water Temperature on Iberian Fish Sprinting Behavior: Volitionally Swimming Performance
Abstract:
Knowing the swimming ability of fish is important to detect movement limitations through hydraulic structures: fish passes weirs and slots, gauging stations, culverts, bridges foundations and other. All these obstacles are collectively referred to velocity barriers . Fish behavior will determine the passage of these structures through combination of fish swimming performance and motivation. Once the fish decides to enter the barrier, must swim faster than the speed of the flow to advance. Thus, the swimming performance depends on the fish speed and fatigue time. To estimate these values, we experimented in an open channel flow with fish samples of different size, swimming volitionally against several high-velocity flows and water temperature range. The movement of fish was controlled by PIT telemetry systems and video record. The information obtained (fish speed, ascend time and distance traveled) was processed using survival analysis techniques and the results can be applied to practical problem solving velocity barriers.
We have worked with two species of Iberian fish: Iberian barbel (Luciobarbus bocagei) and Northern straight-mouth nase (Pseudochondrostoma duriense). The biometrics parameters, flow velocity and water temperature have a significant influence in fish behavior. Endurance and swimming ability of these species do not differ greatly between them and is much higher than the values which are obtained using other methods as respirometers. The results challenge established fish passage guidelines, suggesting that in some cases these species are capable of passing much higher velocities than was previously believed
Manejo de las diferentes esferas en el tratamiento integral del paciente con Siringomielia
Mi Trabajo Fin de Grado está en relación con la Siringomielia, que consiste en una enfermedad caracterizada por la presencia de cavidades quísticas dentro del cordón espinal, que se identifican de forma progresiva, crónica y de curso clínico imprevisible. A partir de toda la información conseguida sobre dicha enfermedad, realizaré un estudio descriptivo basado en una revisión bibliográfica en busca de su origen, su patogenia, incidencia, diagnóstico y tratamiento con el fin de centrar el tema del trabajo y a modo de presentación. Por otro lado, aportaré la visión y los cuidados enfermeros desde todos sus aspectos, valorando al individuo como un ser bio-psico-social, consciente de su enfermedad y con vistas a la mejora de la calidad de vida, por medio de actividades de prevención y promoción de la salud llevadas a cabo por el equipo profesional de manera holística, tanto en zonas de atención especializada, como en Atención Primaria. Con todo esto elaboraremos un plan de cuidados con los recursos disponibles en el entorno sanitario, humanos o materiales, para hacer frente a tal demanda, haciendo hincapié en los objetivos a conseguir y las intervenciones necesarias para ello
Isothermal Titration Calorimetry: Thermodynamic Analysis of the Binding Thermograms of Molecular Recognition Events by Using Equilibrium Models
Fuji-SfM dataset: A collection of annotated images and point clouds for Fuji apple detection and location using structure-from-motion photogrammetry
The present dataset contains colour images acquired in a commercial Fuji apple orchard (Malus domestica Borkh. cv. Fuji) to reconstruct the 3D model of 11 trees by using structure-from-motion (SfM) photogrammetry. The data provided in this article is related to the research article entitled “Fruit detection and 3D location using instance segmentation neural networks and structure-from-motion photogrammetry” [1]. The Fuji-SfM dataset includes: (1) a set of 288 colour images and the corresponding annotations (apples segmentation masks) for training instance segmentation neural networks such as Mask-RCNN; (2) a set of 582 images defining a motion sequence of the scene which was used to generate the 3D model of 11 Fuji apple trees containing 1455 apples by using SfM; (3) the 3D point cloud of the scanned scene with the corresponding apple positions ground truth in global coordinates. With that, this is the first dataset for fruit detection containing images acquired in a motion sequence to build the 3D model of the scanned trees with SfM and including the corresponding 2D and 3D apple location annotations. This data allows the development, training, and test of fruit detection algorithms either based on RGB images, on coloured point clouds or on the combination of both types of data. Dades primàries associades a l'article http://hdl.handle.net/10459.1/68505This work was partly funded by the Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya (grant 2017 SGR 646), the Spanish Ministry of Economy and Competitiveness (project AGL2013-48297-C2-2-R) and the Spanish Ministry of Science, Innovation and Universities (project RTI2018-094222-B-I00). Part of the work was also developed within the framework of the project TEC2016-75976-R, financed by the Spanish Ministry of Economy, Industry and Competitiveness and the European Regional Development Fund (ERDF). The Spanish Ministry of Education is thanked for Mr. J. Gené’s pre-doctoral fellowships (FPU15/03355)
Use of room temperature ionic liquids for the selective fractionation of bioactive ketoses from aldoses
This work deals with the effective fractionation of bioactive ketoses, i.e. lactulose and tagatose, from their corresponding aldoses, lactose and galactose, in equimolar binary mixtures driven by room temperature ionic liquids, i.e. 1-ethyl-3-methylimidazolium dicyanamide ([EMIM][DCA]) and 1-butyl-3-methylimidazolium methyl sulfate ([BMIM][MeSO4]), respectively. Under assayed conditions, tagatose was found to be 6-fold more soluble on [BMIM][MeSO4] than galactose; meanwhile lactulose was 3 times more soluble than lactose on [EMIM][DCA]. As an application example in a more complex sample, a lactose isomerization mixture containing in addition lactulose and monosaccharides was enriched in this ketose by using [EMIM][DCA]. Carbohydrates were then successfully recovered from the ionic liquid following an activated charcoal-based treatment. Overall, lactulose content was enriched from a 24% in the initial isomerization reaction mixture to a 62% in the purified sample. These experimental results demonstrated the potential of ionic liquids as green alternative solvents for the selective fractionation of bioactive ketoses from their corresponding aldoses in food and beverage production.Authors thank Ministerio de Ciencia e Innovación (projects AGL2009-11909 and AGL2011-27884), Ministerio de Economía y Competitividad (project CTQ2012-32957) and the European founding from FEDER program for financial support. L. Ruiz-Aceituno is supported by CSIC by a JAE-Pre grant, co-financed by the European Social Fund (ESF). C. Carrero-Carralero thanks Ministerio de Ciencia e Innovación (Spain) for a predoctoral contract.Peer Reviewe
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