Non-maximal sensitivity to synchronism in periodic elementary cellular automata: exact asymptotic measures

In [11] and [13] the authors showed that elementary cellular automata rules 0, 3, 8, 12, 15, 28, 32, 34, 44, 51, 60, 128, 136, 140, 160, 162, 170, 200 and 204 (and their conjugation, reflection, reflected-conjugation) are not maximum sensitive to synchronism, i.e. they do not have a different dynamics for each (non-equivalent) block-sequential update schedule (defined as ordered partitions of cell positions). In this work we present exact measurements of the sensitivity to synchronism for these rules, as functions of the size. These exhibit a surprising variety of values and associated proof methods, such as the special pairs of rule 128, and the connection to the bissection of Lucas numbers of rule 8

Microbial biomass and soil chemical properties under different land use systems in Northeastern Pará.

parte, à expansão da fronteira agrícola, utilizando áreas já antropizadas ou avançando sobre a vegetação primária. Ao mesmo tempo, os sistemas agrícolas, na pequena produção, continuam utilizando o fogo no preparo da área, o que leva à perda da capacidade produtiva dos solos em curto espaço de tempo, forçando a abertura de novas áreas. Este trabalho avaliou o efeito de métodos de preparo do solo e tempo de pousio que envolvem queima e trituração da vegetação, com permanência na superfície ou incorporada ao solo, com ou sem adubação mineral, em duas épocas do ano sobre os atributos químicos e biológicos do solo. O experimento foi instalado em 1995 em um Latossolo Amarelo do campo experimental da Embrapa Amazônia Oriental, no nordeste do Estado do Pará. O delineamento experimental foi em blocos casualizados, arranjados em esquema fatorial 2 x 6, sendo dois sistemas de manejo e seis tratamentos, estudados em duas épocas de coleta. Os sistemas de manejo envolveram as culturas de arroz (Oriza sativa), seguido de feijão-caupi (Vigna unguiculata) e mandioca (Manihot esculenta). Um sistema constou de dois ciclos de cultivo seguidos, deixando em pousio por três anos; e o outro, de um ciclo de cultivo, deixando em pousio por três anos. Os tratamentos foram: corte e queima da vegetação, com adubação NPK (Q+NPK); corte e queima da vegetação, sem adubação NPK (Q-NPK); corte e trituração da vegetação, deixando-a na superfície do solo, com adubação NPK (C+NPK); corte e trituração da vegetação, deixando-a na superfície do solo, sem adubação NPK (C-NPK); corte e trituração da vegetação, com incorporação e com adubação NPK (I+NPK); e corte e trituração da vegetação, com incorporação e sem adubação NPK (I-NPK). As coletas de solo foram realizadas na estação mais chuvosa (abril de 2006) e na menos chuvosa (setembro de 2006), na profundidade de 0,0?0,1 m. Em cada parcela, foram coletadas 10 amostras simples para compor uma amostra composta. O sistema de manejo mais intensivo apresentou maiores teores de C microbiano (Cmic) e N microbiano (Nmic), ao passo que o sistema menos intensivo mostrou maio teor de C orgânico. Os tratamentos que apresentaram maior teor de Cmic e Nmic foram aqueles em que houve corte, trituração e deposição da biomassa na superfície do solo. Os atributos químicos nos dois sistemas de manejo encontram-se em faixas que enquadram os solos como de baixa fertilidade; no entanto, P e K (no período chuvoso) foram mais elevados no sistema de manejo menos intensivo

Targeting liver stage malaria with metformin

Copyright: © 2019, American Society for Clinical InvestigationDespite an unprecedented 2 decades of success, the combat against malaria - the mosquito-transmitted disease caused by Plasmodium parasites - is no longer progressing. Efforts toward eradication are threatened by the lack of an effective vaccine and a rise in antiparasite drug resistance. Alternative approaches are urgently needed. Repurposing of available, approved drugs with distinct modes of action are being considered as viable and immediate adjuncts to standard antimicrobial treatment. Such strategies may be well suited to the obligatory and clinically silent first phase of Plasmodium infection, where massive parasite replication occurs within hepatocytes in the liver. Here, we report that the widely used antidiabetic drug, metformin, impairs parasite liver stage development of both rodent-infecting Plasmodium berghei and human-infecting P. falciparum parasites. Prophylactic treatment with metformin curtails parasite intracellular growth in vitro. An additional effect was observed in mice with a decrease in the numbers of infected hepatocytes. Moreover, metformin provided in combination with conventional liver- or blood-acting antimalarial drugs further reduced the total burden of P. berghei infection and substantially lessened disease severity in mice. Together, our findings indicate that repurposing of metformin in a prophylactic regimen could be considered for malaria chemoprevention.This work was supported by Fundação para a Ciência e Tecnologia (Portugal) PTDC/SAU-MET/118199/2010 to LMS and European Research Council Proof of Concept Grant to MMM (ERC-2015-PoC-DL3–713691-REUSE4MALARIA).info:eu-repo/semantics/publishedVersio

Características químicas de um gleissolo sob diferentes sistemas de uso, nas margens do rio Guamá, Belém, Pará.

Foram estudados os efeitos do uso do solo sobre a fertilidade, em Gleissolo sob três diferentes sistemas de manejo. As coletas foram feitas antes do período chuvoso nos seguintes sistemas de manejo: área cultivada com arroz (Oriza sativa L.) há aproximadamente 40 anos, com algumas interrupções ao longo deste período, com o cultivo algumas vezes mecanizado e uso de aração e gradagem; área sob pastagem de canarana erecta lisa (Echinochloa pyramidalis Hitch.), formada há cerca de 20 anos sem uso de adubações ou calagens; e área sob vegetação natural de floresta típica das áreas de várzea alta. As amostras de solo foram coletadas em dez pontos a partir de um transecto nas áreas, em quatro profundidades 0-10, 10-20, 20-30 e 30-40 cm. Os resultados demonstraram que o solo em condições naturais, de modo geral, apresentou atributos químicos que variaram de bons a muito bons, o que demonstra um grande potencial para o uso agrícola. O cultivo agrícola dos solos das várzeas do rio Guamá provocou uma redução da concentração de P e de K. O sistema de uso com pastagem apresentou maior sustentabilidade da fertilidade do que o sistema sob cultivo com arroz, visto que, além da melhoria das características químicas, a matéria orgânica elevou-se, também, em relação ao sistema natural

Determination of the neutron star mass-radii relation using narrow-band gravitational wave detector

The direct detection of gravitational waves will provide valuable astrophysical information about many celestial objects. The most promising sources of gravitational waves are neutron stars and black holes. These objects emit waves in a very wide spectrum of frequencies determined by their quasi-normal modes oscillations. In this work we are concerned with the information we can extract from f and p$_I$-modes when a candidate leaves its signature in the resonant mass detectors ALLEGRO, EXPLORER, NAUTILUS, MiniGrail and SCHENBERG. Using the empirical equations, that relate the gravitational wave frequency and damping time with the mass and radii of the source, we have calculated the radii of the stars for a given interval of masses $M$ in the range of frequencies that include the bandwidth of all resonant mass detectors. With these values we obtain diagrams of mass-radii for different frequencies that allowed to determine the better candidates to future detection taking in account the compactness of the source. Finally, to determine which are the models of compact stars that emit gravitational waves in the frequency band of the mass resonant detectors, we compare the mass-radii diagrams obtained by different neutron stars sequences from several relativistic hadronic equations of state (GM1, GM3, TM1, NL3) and quark matter equations of state (NJL, MTI bag model). We verify that quark stars obtained from MIT bag model with bag constant equal to 170 MeV and quark of matter in color-superconductivity phase are the best candidates for mass resonant detectors.Comment: 10 pages and 3 figure

Formation of Temporary Negative Ions and Their Subsequent Fragmentation upon Electron Attachment to CoQ0 and CoQ0H2

P30332 153377/2016‐0 304571/2018‐0 PTDC/FIS‐AQM/31215/2017 PD/00193/2012 UID/FIS/00068/2020 PD/BD/142768/2018Ubiquinone molecules have a high biological relevance due to their action as electron carriers in the mitochondrial electron transport chain. Here, we studied the dissociative interaction of free electrons with CoQ0, the smallest ubiquinone derivative with no isoprenyl units, and its fully reduced form, 2,3-dimethoxy-5-methylhydroquinone (CoQ0H2), an ubiquinol derivative. The anionic products produced upon dissociative electron attachment (DEA) were detected by quadrupole mass spectrometry and studied theoretically through quantum chemical and electron scattering calculations. Despite the structural similarity of the two studied molecules, remarkably only a few DEA reactions are present for both compounds, such as abstraction of a neutral hydrogen atom or the release of a negatively charged methyl group. While the loss of a neutral methyl group represents the most abundant reaction observed in DEA to CoQ0, this pathway is not observed for CoQ0H2. Instead, the loss of a neutral OH radical from the CoQ0H2 temporary negative ion is observed as the most abundant reaction channel. Overall, this study gives insights into electron attachment properties of simple derivatives of more complex molecules found in biochemical pathways.publishersversionpublishe

Formation of Temporary Negative Ions and Their Subsequent Fragmentation upon Electron Attachment to CoQ0 and CoQ0 H2

Publisher Copyright: © 2022 Wiley-VCH GmbH.The front cover artwork is provided by Prof. Stephan Denifl's group at the University of Innsbruck, Austria, in collaboration with Prof. Filipe Ferreira da Silva's group at NOVA University Lisbon, Portugal, and Prof. Dr. Márcio Varella's group at University of São Paulo, Brazil, as well as with Prof. Dr. Martin Beyer and Prof. Dr. Milan Ončak also at the University of Innsbruck, Austria. The image shows the main fragmentation pathways of both coenzyme Q0 (CoQ0 ) and CoQ0 H2 upon electron attachment in the gas phase. Read the full text of the Research Article at 10.1002/cphc.202100834.publishersversionpublishe

Distinct Binding and Immunogenic Properties of the Gonococcal Homologue of Meningococcal Factor H Binding Protein

Neisseria meningitidis is a leading cause of sepsis and meningitis. The bacterium recruits factor H (fH), a negative regulator of the complement system, to its surface via fH binding protein (fHbp), providing a mechanism to avoid complement-mediated killing. fHbp is an important antigen that elicits protective immunity against the meningococcus and has been divided into three different variant groups, V1, V2 and V3, or families A and B. However, immunisation with fHbp V1 does not result in cross-protection against V2 and V3 and vice versa. Furthermore, high affinity binding of fH could impair immune responses against fHbp. Here, we investigate a homologue of fHbp in Neisseria gonorrhoeae, designated as Gonococcal homologue of fHbp (Ghfp) which we show is a promising vaccine candidate for N. meningitidis. We demonstrate that Gfhp is not expressed on the surface of the gonococcus and, despite its high level of identity with fHbp, does not bind fH. Substitution of only two amino acids in Ghfp is sufficient to confer fH binding, while the corresponding residues in V3 fHbp are essential for high affinity fH binding. Furthermore, immune responses against Ghfp recognise V1, V2 and V3 fHbps expressed by a range of clinical isolates, and have serum bactericidal activity against N. meningitidis expressing fHbps from all variant groups