Circularly polarized microwaves for magnetic resonance study in the GHz range: application to nitrogen-vacancy in diamonds

The ability to create time-dependent magnetic fields of controlled polarization is essential for many experiments with magnetic resonance. We describe a microstrip circuit that allows us to generate strong magnetic field at microwave frequencies with arbitrary adjusted polarization. The circuit performance is demonstrated by applying it to an optically detected magnetic resonance and Rabi nutation experiments in nitrogen-vacancy color centers in diamond. Thanks to high efficiency of the proposed microstrip circuit and degree of circular polarization of 85% it is possible to address the specific spin states of a diamond sample using a low power microwave generator.Comment: 4 pages, 7 figures, nitrogen-vacancy, microwave circular polarization, spin-state addressin

Exploring risk factors at the molecular level

Risk factors for cardiovascular diseases trigger molecular changes that harm the endothelial cells in the heart, but exercise can suppress these effects

A fast and reliable method for the delineation of tree crown outlines for the computation of crown openness values and other crown parameters

Numerous crown parameters (e.g., leaf area index, diameter, height, volume) can be obtained via the analysis of tree crown photographs. In all cases, parameter values are functions of the position of the crown outline. However, no standardized method to delineate crowns exists. To explore the effect of different outlines on tree crown descriptors, in this case crown openness (CO), and facilitate the adoption of a standard method free of user bias, we developed the program Crown Delineator that automatically delineates any outline around tree crowns following predetermined sensibility settings. We used different outlines to analyze tree CO in contrasting settings: using saplings from four species in young boreal mixedwood forests and medium-sized hybrid poplar trees from a low-density plantation. In both cases, the estimated CO increases when calculated from a looser outline, which had a strong influence on understory available light simulations using a forest simulator. These results demonstrate that the method used to trace crown outlines is an important step in the determination of CO values. We provide a much-needed computer-assisted solution to help standardize this procedure, which can also be used in many other situations in which the delineation of tree crowns is needed (e.g., competition and crown shyness)

Coherent population oscillations with nitrogen-vacancy color centers in diamond

We present results of our research on two-field (two-frequency) microwave spectroscopy in nitrogen-vacancy (NV-) color centers in a diamond. Both fields are tuned to transitions between the spin sublevels of the NV- ensemble in the 3A2 ground state (one field has a fixed frequency while the second one is scanned). Particular attention is focused on the case where two microwaves fields drive the same transition between two NV- ground state sublevels (ms=0 -> ms=+1). In this case, the observed spectra exhibit a complex narrow structure composed of three Lorentzian resonances positioned at the pump-field frequency. The resonance widths and amplitudes depend on the lifetimes of the levels involved in the transition. We attribute the spectra to coherent population oscillations induced by the two nearly degenerate microwave fields, which we have also observed in real time. The observations agree well with a theoretical model and can be useful for investigation of the NV relaxation mechanisms.Comment: 17 page

Microwave saturation spectroscopy of nitrogen-vacancy ensembles in diamond

Negatively-charged nitrogen-vacancy (NV$^-$) centers in diamond have generated much recent interest for their use in sensing. The sensitivity improves when the NV ground-state microwave transitions are narrow, but these transitions suffer from inhomogeneous broadening, especially in high-density NV ensembles. To better understand and remove the sources of broadening, we demonstrate room-temperature spectral "hole burning" of the NV ground-state transitions. We find that hole burning removes the broadening caused by magnetic fields from $^{13}$C nuclei and demonstrate that it can be used for magnetic-field-insensitive thermometry.Comment: Main text: 5 pages, 4 figures. Supplement: 6 pages, 3 figure

Invariant density and time asymptotics for collisionless kinetic equations with partly diffuse boundary operators

International audienceThis paper deals with collisionless transport equationsin bounded open domains $\Omega \subset \R^{d}$ $(d\geq 2)$ with $\mathcal{C}^{1}$ boundary $\partial \Omega$, orthogonallyinvariant velocity measure \bm{m}(\d v) with support $V\subset \R^{d}$ and stochastic partly diffuse boundary operators $\mathsf{H}$ relating the outgoing andincoming fluxes. Under very general conditions, such equations are governedby stochastic $C_{0}$-semigroups $\left( U_{\mathsf{H}}(t)\right) _{t\geq 0}$ on $$ We give a general criterion of irreducibility of $$ and we show that, under very natural assumptions, if an invariant densityexists then $\left( U_{\mathsf{H}}(t)\right) _{t\geq 0}$ converges strongly (notsimply in Cesar\`o means) to its ergodic projection. We show also that if noinvariant density exists then $\left( U_{\mathsf{H}}(t)\right) _{t\geq 0}$ is\emph{sweeping} in the sense that, for any density $\varphi$, the total mass of $$ concentrates near suitable sets of zero measure as $$ We show also a general weak compactness theoremwhich provides a basis for a general theory on existence of invariantdensities. This theorem is based on a series of results on smoothness andtransversality of the dynamical flow associated to $\left( U_{\mathsf{H}}(t)\right) _{t\geq0}.

Histopathological evaluation of recurrent goiter.

The recurrent goiter is the regrowth of thyroid tissue after thyroidectomy. An inadequate surgical removal of the thyroid gland, lack of substitution therapy and pathological stimulation of the thyroid growth can all promote the recurrence. The aim of this study was to find the connection between the histopathological findings during the first and second operation and the recurrence of goiter. The study group consisted of 29 women and 1 man. The mean time to recurrence was 15 years. The most frequent histopathological finding during the first and second operation was struma nodosa. According to our observations different histopathological findings were found in 63.4% cases after primary and secondary thyroidectomy. Some genetic investigations showed that nodules in recurrent goiters did not derive from nodules left during the first operation but from a group of cells which had high growth potential. Thus, not only the operation technique and substitution after operation are key factors of successful therapy of goiter, but also other factors which stimulate the re-growth of thyroid tissue

Canonical Wnt Signaling Induces BMP-4 to Specify Slow Myofibrogenesis of Fetal Myoblasts

Background The Wnts are secreted proteins that play important roles in skeletal myogenesis, muscle fiber type diversification, neuromuscular junction formation and muscle stem cell function. How Wnt proteins orchestrate such diverse activities remains poorly understood. Canonical Wnt signaling stabilizes β-catenin, which subsequently translocate to the nucleus to activate the transcription of TCF/LEF family genes. Methods We employed TCF-reporter mice and performed analysis of embryos and of muscle groups. We further isolated fetal myoblasts and performed cell and molecular analyses. Results We found that canonical Wnt signaling is strongly activated during fetal myogenesis and weakly activated in adult muscles limited to the slow myofibers. Muscle-specific transgenic expression of a stabilized β-catenin protein led to increased oxidative myofibers and reduced muscle mass, suggesting that canonical Wnt signaling promotes slow fiber types and inhibits myogenesis. By TCF-luciferase reporter assay, we identified Wnt-1 and Wnt-3a as potent activators of canonical Wnt signaling in myogenic progenitors. Consistent with in vivo data, constitutive overexpression of Wnt-1 or Wnt-3a inhibited the proliferation of both C2C12 and primary myoblasts. Surprisingly, Wnt-1 and Wnt-3a overexpression up-regulated BMP-4, and inhibition of BMP-4 by shRNA or recombinant Noggin protein rescued the myogenic inhibitory effect of Wnt-1 and Wnt-3a. Importantly, Wnt-3a or BMP-4 recombinant proteins promoted slow myosin heavy chain expression during myogenic differentiation of fetal myoblasts. Conclusions These results demonstrate a novel interaction between canonical Wnt and BMP signaling that induces myogenic differentiation towards slow muscle phenotype

Computational Analysis of Molecular Interaction Networks Underlying Change of HIV-1 Resistance to Selected Reverse Transcriptase Inhibitors

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