242 research outputs found
Valoración Radiológica del Fracaso de las Prótesis Cervico-Cefálicas de Cadera en Fracturas de Cuello Femoral y Cirugía de Revisión
Lo s autore s analiza n los diferentes signos radiológicos que sugieren intolerancia
de la s prótesis cérvico-cefálicas de cader a implantada s po r fractur a cervical que será n
sometida s a Cirugí a de Revisión. Se estudian retrospectivamente 61 casos intervenidos entre los
años 77 al 89, analizándos e la evolución de los diferentes modelos: Monk, Austin-Moore,
Thompson, Mülle r y Robert-Mathys.
Asimismo se estudia n la presencia de calcificaciones intr a y periarticulare s y la aparició n de
complicaciones. Po r último, se pretende concretar la indicación de la Artroplasti a parcia l de
cader a en paciente s anciano s con alto riesgo quirúrgico en oposición a un mejor resultad o de la
Artroplasti a Total en el resto de pacientes.The author s analys e the different radiologica l feature s suggestin g
intoleranc e o f the cervical-cephali c hi p prosthesis, implante d b y cervica l fractur e
a n d submitte d t o revisio n surgery. Retrospectivel y 6 1 cases, whic h wer e carrie d ou t
betwee n 197 7 an d 1989, ar e studied, an d the evolutio n o f the different pattern s i n
analysed : Monk, Austin-Moore , Thompson , Mülle r an d Robert-Mathys.
A t the sam e time , the presenc e o f intr a an d peri-articula r calcification s an d the
appearanc e o f complication s ar e examined . Finally , they inten d t o summariz e th e indicatio
n o f the hi p hemiarthroplast y i n ol d patien s wit h a hig h surgica l risk oppositio
n t o a bette r result o f the tota l hi p arthroplast y in the rest o f th e patients
“Anti-Michael addition” of Grignard reagents to sulfonylacetylenes: synthesis of alkynes
In this work, the addition of Grignard reagents to arylsulfonylacetylenes, which undergoes an “anti-Michael addition”, resulting in their alkynylation under very mild conditions is described. The simplicity of the experimental procedure and the functional group tolerance are the main features of this methodology. This is an important advantage over the use of organolithium at −78 °C that we previously reported. Moreover, the synthesis of diynes and other examples showing functional group tolerance in this anti-Michael reaction is also presentedFinancial support from the Spanish Government CTQ2012- 35957 (MINECO) and CTQ2015-64561-R (MINECO/FEDER) is gratefully acknowledged. F. E. is thankful to the Spanish government for a FPI fellowship, and L. B. is thankful to the Université Badji Mokhtar Annaba for a predoctoral stay gran
Stereochemical aspects and the synthetic scope of the SHi at the sulfur atom. Preparation of enantiopure 3-substituted 2,3-dihydro-1,2- benzoisothiazole 1-oxides and 1,1-dioxides
Intramolecular homolytic substitution (SHi) on the sulfur atom at acyclic N-(o-bromobenzyl)sulfinamides takes place with a complete inversion of the configuration and
provides an excellent tool to connect N-tert-butanesulfinylimines with enantiopure 3-substituted benzo-fused sulfinamides (1,2-benzoisothiazoline 1-oxides) and the related pharmacologically relevant sulfonamidesThe Spanish Government (grant CTQ2012-35957) and Comunidad de Madrid (CCG08-UAM/PPQ-4151; S2009/PPQ1634
Aislamiento y cultivo de células osteoblásticas: Interés para la investigación en cirugía ortopédica y traumatología
Describimos un método de aislamiento y caracterización de células osteoblásticas
procedentes de la superficie de trabéculas óseas de explantes obtenidos de metafisis de
fémures y tibias de conejo y de cabezas femorales humanas de pacientes sometidos a cirugía
protésica de cadera. Después de eliminar las células de la médula ósea de los explantes, estos
se despositaron sobre dos tipos de mallas, de 80 y 100 µm., obteniéndose un cultivo en
monocarpa que se mostró confluente a partir de los 20 días. La población celular aislada
presentó un fenotipo osteoblástico utilizando métodos colorimétricos para determinación de
la actividad de fosfatasa alcalina (FA) y morfológicos por medio de la observación con microscopía
electrónica de barrido. Las células cultivadas exhibieron una morfología diferente
según el grado de confluencia de cultivo, siendo en todo momento positivas las sucesivas determinaciones
colorimétricas de FA realizadas a lo largo de la experiencia. Consideramos el
método de gran interés en investigación en ortopédica, pues permite el estudio de comportamiento
de estas células cuando están sometidas a cualquier tipo de estímulo externo, así como
cuando se enfrentan a nuevas generaciones de biomateriales, etc.We describe a method of isolation and characterization of osteoblastic cells taken
from the surface of bone trabeculae of explants obtained from femur and tibia metaphysis
in rabbits and human femoral head in patients undergoing orthopaedic surgery. Afther removing
the cells from the bone marrow of explants, they were placed over two mesh, 80 and
100 µmn, obtaining then a culture in monolayer which showed to be confluent after 20 days.
The isolated cell population showed an osteoblastic phenotype using colourmetric methods to
determinate the alkaline phosphatase (AF) activity and morphologic methods by means of
scanning. The cultivated cell showed a different morphology according to the degree of confluence
of the culture, being always positive the sucesive colourmetric determinations of AF
along the experiment. We consider the method of a great interest for orthopaedic investigations,
since it allows the study of the behaviour of these cells when they are subjet to different
types of external stimulus as well as meet they the new generations of biomaterials
Small-animal PET registration method with intrinsic validation designed for large datasets
Proceeding of: 2007 IEEE Nuclear Science Symposium Conference Record (NSS'07), Honolulu, Hawaii, USA, Oct. 27 - Nov. 3, 2007We present a procedure to validate the results of small animal Positron Emission Tomography (PET) image registration by means of consistency measures. Small animal 2-Deoxy-2-[F-18]fluoro-D-glucose (FDG) PET studies do not show the same intensity distribution even for images acquired in
similar conditions, as the resulting image is influenced by several variables which are not always completely under control.
Because of these difficulties, the results from automatic registration methods have to be visually inspected to detect failures. We propose a method to automate this validation
process. Two reference images from the dataset are selected by an expert user avoiding images with poor contrast, animal
movement or low quality, and both are co-registered using anatomical landmarks. All the remaining images in the dataset
are then registered to every reference with an automatic two-step algorithm based on Mutual Information. The known transformation relating both references allows measuring the
registration consistency, which is a good estimator of the accuracy of the alignment process, for every image in the dataset.
This value can be used to assess the quality of the registration and therefore detect the incorrect results. We have applied this
validation process on a large dataset of 120 FDG-PET rat brain images obtained with a rotating PET scanner. The registration
consistency was calculated for every image in the dataset and values below 1.65 mm (PET image resolution) were considered as
successful registrations. 116 images were correctly registered with an average error of 0.839 mm, while in four images the
proposed method detected a registration failure. Two of these failures were due to very low image quality and these studies
were discarded from the study, while the other two were correctly aligned after applying a manual pre-alignment step.
Our approach requires minimal user interaction and provides automatic assessment of the registration error, making it unnecessary to visually inspect and check every registration result.This work was supported by projects CIBER CB06/01/0079 (Ministerio de Sanidad y Consumo) and CDTEAM (CENIT program, Ministerio de Industria)
A pilot 1-year follow-up randomised controlled trial comparing metacognitive training to psychoeducation in schizophrenia: effects on insight
Poor insight in schizophrenia spectrum disorders (SSD) is linked with negative outcomes. This single-centre, assessor-blind, parallel-group 1-year follow-up randomised controlled trial (RCT) tested whether metacognitive training (MCT) (compared to psychoeducation) may improve insight and outcomes in outpatients with SSD assessed: at baseline (T0); after treatment (T1) and at 1-year follow-up (T2). Insight (primary outcome) was measured with (i) the Schedule for Assessment of Insight-Expanded version- (SAI-E), including illness recognition (IR), symptom relabelling (SR), treatment compliance (TC) and total insight scores (TIS); and (ii) the Beck Cognitive Insight Scale (BCIS). Between-group comparisons were nonsignificant, while within the MCT group (but not within controls) there was a significant medium effect size for improved TIS at T2 (d = 0.67, P = 0.02). Secondary outcomes included cognitive measures: Jumping to Conclusions (JTC), Theory of Mind (ToM), plus symptom severity and functioning. Compared to psychoeducation, MCT improved the PANSS excitement (d = 1.21, P = 0.01) and depressed (d = 0.76, P = 0.05) factors at T2; and a JTC task both at T1 (P = 0.016) and at T2 (P = 0.031). Participants in this RCT receiving MCT showed improved insight at 1-year follow-up, which was associated with better mood and reduced JTC cognitive bias. In this pilot study, no significant benefits on insight of MCT over psychoeducation were detected, which may have been due to insufficient power
Can metacognitive interventions improve insight in schizophrenia spectrum disorders? A systematic review and meta-analysis
Background:
Patients with schizophrenia spectrum disorders (SSD) tend to lack insight, which is linked to poor outcomes. The effect size of previous treatments on insight changes in SSD has been small. Metacognitive interventions may improve insight in SSD, although this remains unproved.
Methods:
We carried out a systematic review and meta-analysis of randomized controlled trials (RCTs) to examine the effects of metacognitive interventions designed for SSD, namely Metacognitive Training (MCT) and Metacognitive Reflection and Insight Therapy (MERIT), on changes in cognitive and clinical insight at post-treatment and at follow-up.
Results:
Twelve RCTs, including 10 MCT RCTs (n = 717 participants) and two MERIT trials (n = 90), were selected, totalling N = 807 participants. Regarding cognitive insight six RCTs (n = 443) highlighted a medium effect of MCT on self-reflectiveness at post-treatment, d = 0.46, p < 0.01, and at follow-up, d = 0.30, p < 0.01. There was a small effect of MCT on self-certainty at post-treatment, d = −0.23, p = 0.03, but not at follow-up. MCT was superior to controls on an overall Composite Index of cognitive insight at post-treatment, d = 1.11, p < 0.01, and at follow-up, d = 0.86, p = 0.03, although we found evidence of heterogeneity. Of five MCT trials on clinical insight (n = 244 participants), which could not be meta-analysed, four of them favoured MCT compared v. control. The two MERIT trials reported conflicting results.
Conclusions:
Metacognitive interventions, particularly Metacognitive Training, appear to improve insight in patients with SSD, especially cognitive insight shortly after treatment. Further long-term RCTs are needed to establish whether these metacognitive interventions-related insight changes are sustained over a longer time period and result in better outcomes
IL6/sIL6R Regulates TNFα-Inflammatory Response in Synovial Fibroblasts Through Modulation of Transcriptional and Post-Transcriptional Mechanisms
[Abstract] Introduction: The clinical efficacy of specific interleukin-6 inhibitors has confirmed the central role of IL6 in rheumatoid arthritis (RA). However the local role of IL6, in particular in synovial fibroblasts (SF) as a direct cellular target to IL6/sIL6R signal is not well characterized. The purpose of the study was to characterize the crosstalk between TNFα and IL6/sIL6R signaling to the effector pro-inflammatory response of SF.
Methods: SF lines were stimulated with either TNFα, IL6/sIL6R, or both together, for the time and dose indicated for each experiment, and where indicated, cells were treated with inhibitors actinomycin D, adalimumab, ruxolitinib and cycloheximide. mRNA expression of cytokines, chemokines and matrix metalloproteases (MMPs) were analyzed by quantitative RT-PCR. Level of IL8/CXCL8 and CCL8 in culture supernatants was measured by ELISA. Mononuclear and polymorphonuclear cells migration assays were assessed by transwell using conditioned medium from SF cultures. Statistical analyses were performed as indicated in the corresponding figure legends and a p-value < 0.05 was considered statistically significant.
Results: The stimulation of SF with IL6/sIL6R and TNFα, cooperatively promotes the expression of mono- and lymphocytic chemokines such as IL6, CCL8 and CCL2, as well as matrix degrading enzymes such as MMP1, while inhibiting the induction of central neutrophil chemokines such as IL8/CXCL8. These changes in the pattern of chemokines expression resulted in reduced polymorphonuclear (PMN) and increased mononuclear cells (MNC) chemoattraction by SF. Mechanistic analyses of the temporal expression of genes demonstrated that the cooperative regulation mediated by these two factors is mostly induced through de novo transcriptional mechanisms activated by IL6/sIL6R. Furthermore, we also demonstrate that TNFα and IL6/sIL6R cooperation is partially mediated by the expression of secondary factors signaling through JAK/STAT pathways.
Conclusions: These results point out to a highly orchestrated response to IL6 in TNFα-induced SF and provide additional insights into the role of IL6/sIL6R in the context of RA, highlighting the contribution of IL6/sIL6R to the interplay of SF with other inflammatory cells.Instituto de Salud Carlos III; FIS 16/00032Instituto de Salud Carlos III; RETICS RD16/0012 RIE
Compilation of parameterized seismogenic sources in Iberia for the SHARE European-scale seismic source model.
Abstract: SHARE (Seismic Hazard Harmonization in Europe) is an EC-funded project (FP7) that aims to evaluate European seismic hazards using an integrated, standardized approach. In the context of SHARE, we are compiling a fully-parameterized active fault database for Iberia and the nearby offshore region. The principal goal of this initiative is for fault sources in the Iberian region to be represented in SHARE and incorporated into the source model that will be used to produce seismic hazard maps at the European scale. The SHARE project relies heavily on input from many regional experts throughout the Euro-Mediterranean region. At the SHARE regional meeting for Iberia, the 2010 Working Group on Iberian Seismogenic Sources (WGISS) was established; these researchers are contributing to this large effort by providing their data to the Iberian regional integrators in a standardized format. The development of the SHARE Iberian active fault database is occurring in parallel with IBERFAULT, another ongoing effort to compile a database of active faults in the Iberian region.
The SHARE Iberian active fault database synthesizes a wide range of geological and geophysical observations on active seismogenic sources, and incorporates existing compilations (e.g., Cabral, 1995; Silva et al., 2008), original data contributed directly from researchers, data compiled from the literature, parameters estimated using empirical and analytical relationships, and, where necessary, parameters derived using expert judgment. The Iberian seismogenic source model derived for SHARE will be the first regional-scale source model for Iberia that includes fault data and follows an internationally standardized approach (Basili et al., 2008; 2009). This model can be used in both seismic hazard and risk analyses and will be appropriate for use in Iberian- and European-scale assessments
Compilation of parameterized seismogenic sources in Iberia for the SHARE European-scale seismic source model
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