Microtubule cytoskeleton perturbation induced by taxol and colchicine affects chaperonin containing TCP-1 (CCT) subunit gene expression in Tetrahymena cells

We report the existence of a CCTϵ subunit gene that encodes subunit ϵ of the chaperonin CCT (chaperonin containing TCP-1) in Tetrahymena pyriformis. This work focuses on the study of the effects of the microtubule polymerizing agent taxol and the depolymerizing agent colchicine on microtubule dynamics and their role in the regulation of tubulin and CCT subunit genes. Under taxol treatment some TpCCT and tubulin genes are distinctly expressed until 30 min of treatment. Cytoplasmic TpCCT mRNA levels slightly decrease while tubulin transcripts are increasing. In colchicine treated cells TpCCT and tubulin transcripts decrease in the initial 30 min of treatment and then start to increase. However, both antimitotic agents induce TpCCT and tubulin gene transcription. This induction does not correlate with increased steady-state levels of TpCCT proteins and seems to be necessary to replete cytoplasmic TpCCT mRNAs. Moreover, we found that TpCCTϵ and TpCCTα but not TpCCTη are present in the insoluble fraction after a postmitochondrial fractionation that contains components of the ciliate cortex structure, basal bodies and cilia. This suggests that some TpCCT subunits may be associated with these structures. The association of TpCCTϵ subunit is stimulated either by taxol or colchicine treatment. These observations support the idea that CCT subunits could have additional roles in vivo.info:eu-repo/semantics/publishedVersio

Utilização de indicadores orgânicos de pH no ensino de ácidos e bases: considerando alguns aspectos históricos

..

Mme Curie e o Ensino de Ciências no início do século XX

..

Mediação no Direito do Trabalho: A Necessidade de Adequação à Luz dos Princípios Juslaborais

The paper discusses the suitability of judicial mediation for the solution of labor disputes. Judicial and extrajudicial conflict-solving methods are approached, with emphasis on mediation. Aiming at understanding both the adequacy and application of mediation in Labor Law, the paper examines special principles of this branch, in particular, the principle of unavailability of Labor rights, for which the case of absolute and relative rights are contrasted. Finally, it concludes for the application of mediation to the Collective Labour Law, which is limited to the rights of relative unavailability.O artigo aborda a adequação da mediação ao ramo trabalhista, a partir do estudo inicial das formas de solução de conflitos judicial e extrajudiciais, com ênfase na mediação, analisando esta forma de solução de maneira detida. Com o objetivo de entender a adequação e aplicação da mediação ao Direito do Trabalho, passa à análise dos princípios especiais desse ramo, com destaque ao princípio da indisponibilidade dos direitos trabalhistas e especial atenção à diferenciação entre direitos de indisponibilidade absoluta e relativa. Por fim, conclui-se pela adequação da mediação ao Direito Coletivo do Trabalho, que está limitado aos direitos de indisponibilidade relativa

Substitutos do " novo" mundo para as antigas plantas raras: um estudo de caso dos bálsamos

Convinced that the " true balsam" was lost forever, Conrad Gesner described other substances with similar healing virtues. However, he was not the only one in the 16th to search for other varieties of balsamic oleoresins. The arrival of the Europeans to the Americas allowed the finding of native plants with properties similar to those of the original balsam, including Balsam of Peru, Balsam of Tolu and particularly in the Brazilian area, Balsam of Copaiba. Focusing on the Brazilian context, this paper analyzes two different moments in the transit of the newly found varieties of balsams to the pharmacopeia and materia medica

IFC score set appropriate to asses elderly peoples functionality

Objetivo: Verificar um Score Set que permita uma avaliação da condição do idoso, de forma a identificar os cuidados necessários em pessoas idosas residentes na comunidade. Materiais e Métodos: A metodologia utilizada para a elaboração deste trabalho assenta numa revisão integrativa da literatura. A questão de partida utilizando a estratégia PICO: “Qual é o score set da CIF mais adequado na avaliação da funcionalidade da pessoa idosa residente na comunidade?”. Para verificar a qualidade metodológica, recorreu-se à classificação da JBI (2011) para estudos descritivos e às grelhas de avaliação crítica segundo Bugalho e Carneiro (2004). Quanto aos níveis de evidência e sua classificação, foram utilizadas as recomendações da Registered Nurses' Association of Ontario (2007). Resultados: Foi obtida uma amostra de 4 artigos. Sendo que todos eles abordam as componentes da Atividade e Participação, e Funções e Estruturas Corporais da CIF, mas não se centram nos Fatores Ambientais e Pessoais, que surgem como foco importante na vida do idoso. Conclusões: O envelhecimento é um foco atual de cuidados. A CIF como uma linguagem universal permite-nos uma uniformização dos cuidados e personalização, adequação e monitorização dos mesmos

Building a Portuguese Coalition for Biodiversity Genomics

The diverse physiography of the Portuguese land and marine territory, spanning from continental Europe to the Atlantic archipelagos, has made it an important repository of biodiversity throughout the Pleistocene glacial cycles, leading to a remarkable diversity of species and ecosystems. This rich biodiversity is under threat from anthropogenic drivers, such as climate change, invasive species, land use changes, overexploitation or pathogen (re)emergence. The inventory, characterization and study of biodiversity at inter- and intra-specific levels using genomics is crucial to promote its preservation and recovery by informing biodiversity conservation policies, management measures and research. The participation of researchers from Portuguese institutions in the European Reference Genome Atlas (ERGA) initiative, and its pilot effort to generate reference genomes for European biodiversity, has reinforced the establishment of Biogenome Portugal. This nascent institutional network will connect the national community of researchers in genomics. Here, we describe the Portuguese contribution to ERGA’s pilot effort, which will generate high-quality reference genomes of six species from Portugal that are endemic, iconic and/or endangered, and include plants, insects and vertebrates (fish, birds and mammals) from mainland Portugal or the Azores islands. In addition, we outline the objectives of Biogenome Portugal, which aims to (i) promote scientific collaboration, (ii) contribute to advanced training, (iii) stimulate the participation of institutions and researchers based in Portugal in international biodiversity genomics initiatives, and (iv) contribute to the transfer of knowledge to stakeholders and engaging the public to preserve biodiversity. This initiative will strengthen biodiversity genomics research in Portugal and fuel the genomic inventory of Portuguese eukaryotic species. Such efforts will be critical to the conservation of the country’s rich biodiversity and will contribute to ERGA’s goal of generating reference genomes for European species.info:eu-repo/semantics/publishedVersio

The European Reference Genome Atlas: piloting a decentralised approach to equitable biodiversity genomics.

ABSTRACT: A global genome database of all of Earth’s species diversity could be a treasure trove of scientific discoveries. However, regardless of the major advances in genome sequencing technologies, only a tiny fraction of species have genomic information available. To contribute to a more complete planetary genomic database, scientists and institutions across the world have united under the Earth BioGenome Project (EBP), which plans to sequence and assemble high-quality reference genomes for all ∼1.5 million recognized eukaryotic species through a stepwise phased approach. As the initiative transitions into Phase II, where 150,000 species are to be sequenced in just four years, worldwide participation in the project will be fundamental to success. As the European node of the EBP, the European Reference Genome Atlas (ERGA) seeks to implement a new decentralised, accessible, equitable and inclusive model for producing high-quality reference genomes, which will inform EBP as it scales. To embark on this mission, ERGA launched a Pilot Project to establish a network across Europe to develop and test the first infrastructure of its kind for the coordinated and distributed reference genome production on 98 European eukaryotic species from sample providers across 33 European countries. Here we outline the process and challenges faced during the development of a pilot infrastructure for the production of reference genome resources, and explore the effectiveness of this approach in terms of high-quality reference genome production, considering also equity and inclusion. The outcomes and lessons learned during this pilot provide a solid foundation for ERGA while offering key learnings to other transnational and national genomic resource projects.info:eu-repo/semantics/publishedVersio