7,234 research outputs found
Fast inactivation in Shaker K+ channels. Properties of ionic and gating currents.
Fast inactivating Shaker H4 potassium channels and nonconducting pore mutant Shaker H4 W434F channels have been used to correlate the installation and recovery of the fast inactivation of ionic current with changes in the kinetics of gating current known as "charge immobilization" (Armstrong, C.M., and F. Bezanilla. 1977. J. Gen. Physiol. 70:567-590.). Shaker H4 W434F gating currents are very similar to those of the conducting clone recorded in potassium-free solutions. This mutant channel allows the recording of the total gating charge return, even when returning from potentials that would largely inactivate conducting channels. As the depolarizing potential increased, the OFF gating currents decay phase at -90 mV return potential changed from a single fast component to at least two components, the slower requiring approximately 200 ms for a full charge return. The charge immobilization onset and the ionic current decay have an identical time course. The recoveries of gating current (Shaker H4 W434F) and ionic current (Shaker H4) in 2 mM external potassium have at least two components. Both recoveries are similar at -120 and -90 mV. In contrast, at higher potentials (-70 and -50 mV), the gating charge recovers significantly more slowly than the ionic current. A model with a single inactivated state cannot account for all our data, which strongly support the existence of "parallel" inactivated states. In this model, a fraction of the charge can be recovered upon repolarization while the channel pore is occupied by the NH2-terminus region
Equilibrium onions?
We demonstrate the possibility of a stable equilibrium multi-lamellar ("onion") phase in pure lamellar systems (no excess solvent) due to a sufficiently negative Gaussian curvature modulus. The onion phase is stabilized by non-linear elastic moduli coupled to a polydisperse size distribution (Apollonian packing) to allow space-filling without appreciable elastic distortion. This model is compared to experiments on copolymer-decorated lamellar surfactant systems, with reasonable qualitative agreement
Walls Inhibit Chaotic Mixing
We report on experiments of chaotic mixing in a closed vessel, in which a
highly viscous fluid is stirred by a moving rod. We analyze quantitatively how
the concentration field of a low-diffusivity dye relaxes towards homogeneity,
and we observe a slow algebraic decay of the inhomogeneity, at odds with the
exponential decay predicted by most previous studies. Visual observations
reveal the dominant role of the vessel wall, which strongly influences the
concentration field in the entire domain and causes the anomalous scaling. A
simplified 1D model supports our experimental results. Quantitative analysis of
the concentration pattern leads to scalings for the distributions and the
variance of the concentration field consistent with experimental and numerical
results.Comment: 4 pages, 3 figure
Discrepancy between sub-critical and fast rupture roughness: a cumulant analysis
We study the roughness of a crack interface in a sheet of paper. We
distinguish between slow (sub-critical) and fast crack growth regimes. We show
that the fracture roughness is different in the two regimes using a new method
based on a multifractal formalism recently developed in the turbulence
literature. Deviations from monofractality also appear to be different in both
regimes
Roughness of fracture surfaces
We study the fracture surface of three dimensional samples through a model
for quasi-static fractures known as Born Model. We find for the roughness
exponent a value of 0.5 expected for ``small length scales'' in microfracturing
experiments. Our simulations confirm that at small length scales the fracture
can be considered as quasi-static. The isotropy of the roughness exponent on
the crack surface is also shown. Finally, considering the crack front, we
compute the roughness exponents for longitudinal and transverse fluctuations of
the crack line (both 0.5). They result in agreement with experimental data, and
supports the possible application of the model of line depinning in the case of
long-range interactions.Comment: 10 pages, 5 figures, Late
Slow decay of concentration variance due to no-slip walls in chaotic mixing
Chaotic mixing in a closed vessel is studied experimentally and numerically
in different 2-D flow configurations. For a purely hyperbolic phase space, it
is well-known that concentration fluctuations converge to an eigenmode of the
advection-diffusion operator and decay exponentially with time. We illustrate
how the unstable manifold of hyperbolic periodic points dominates the resulting
persistent pattern. We show for different physical viscous flows that, in the
case of a fully chaotic Poincare section, parabolic periodic points at the
walls lead to slower (algebraic) decay. A persistent pattern, the backbone of
which is the unstable manifold of parabolic points, can be observed. However,
slow stretching at the wall forbids the rapid propagation of stretched
filaments throughout the whole domain, and hence delays the formation of an
eigenmode until it is no longer experimentally observable. Inspired by the
baker's map, we introduce a 1-D model with a parabolic point that gives a good
account of the slow decay observed in experiments. We derive a universal decay
law for such systems parametrized by the rate at which a particle approaches
the no-slip wall.Comment: 17 pages, 12 figure
Cleavage of C3 by Neutral Proteases from Granulocytes in Pleural Empyema
The possibility of direct inactivation of C3 by granular enzymes from polymorphonuclear leukocytes(PMNLs) in pleural empyema was examined. As a group, pleural empyema from 10 patients with purulent effusions and a positive bacteriologic culture cleaved significantly more 125I-labeled C3 bound to Sepharose (18.4% ± 7.3%) than did 19sterile pleural effusions (2.4% ± 0.9%; P << 0.001)and sonicates from bacterial strains commonly found in empyema (1.4% ± 0.2%). Granular enzymesfrom 7 × 106 PMNLs cleaved 78.5% of 125I-labeled C3 bound to Sepharose. When proteolysis of 125I-labeled C3 after incubation with pleural empyema or PMNL granular enzymes was examined with polyacrylamide gel electrophoresis, breakdown products were similar. Granulocyte elastase-like activity was detected in four samples of pleural empyema. Granulocyte elastase inhibitors, as well as 10% human serum, effectively suppressed cleavage of C3 and elastase-like activity. In pleural empyemas, granular enzymes from PMNLs, especially elastase, apparently contribute to low complement-mediated opsonic activity by direct inactivation of C
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