Fluoropyrimidine sensitivity of human MCF-7 breast cancer cells stably transfected with human uridinehosphorylase

The relationship between uridine phosphorylase (UP) expression level in cancer cells and the tumour sensitivity to fluoropyrimidines is unclear. In this study, we found that UP overexpression by gene transfer, and the subsequent efficient metabolic activation of 5-fluorouracil (5-FU) by the ribonucleotide pathway, does not increase the fluoropyrimidine sensitivity of MCF-7 human cancer cells. © 2001 Cancer Research Campaign http://www.bjcancer.co

Cell-Degradation of Calcium Phosphate Ceramics

Calcium phosphate ceramics are used in bone surgery under different forms: dense or porous ceramic s as bone substitute, thin ceramic coatings on metallic implants as an osseointegration enhancer. Their degradation depends on their physico-chemical properties and particularly on their chemical composition. Natural calcium phosphates of bone are degraded by mononuclear or multinuclear cells and the extracellular matrix induces the differentiation of the degrading-cells. Hydroxyapatite, which is one of the most used calcium phosphates , is known as a low degradation material. However, the histological analysis of implanted HA-materials both in animals and in humans showed that a cellular degradation took place on the surface of the material

A comparative study of unsupervised regions segmentation strategies by Markov random fields

This article deals with the important problem of unsupervised segmentation of luminance images based on a markovian relaxation . As these relaxations need important computation times, we have developed a new approach which considerably decreases thes e times, without modify quality of segmentation results . This approach is based on a split and merge technique . The splitting step allows to decrease, in a important way, the number of entities present in the merging step and, thereafter, computation times . The method introduced for the splitting step is original ; it is based on extraction of second order statistics from cooccurrence matrices . The study shows advantages of these statistics, and compares them to those of one order extracted from grey level s histograms . The second point developed in this report concerns the merging step . It is accomplished by a markovian relaxation achieved o n irregular adjacency graph of regions coming from the splitting step . Many original contributions are presented to estimate th e hyper – parameters of the system .Cet article aborde le problème de la segmentation non supervisée par champs de Markov d'images de luminance. L'approche développée est de type division-fusion. L'étape de division, qui est une sursegmentation rapide, permet de diminuer de manière importante le nombre de données présentes dans le processus de fusion et, par la suite, les temps de calcul. La méthode introduite pour la division est basée sur l'extraction de statistiques d'ordre deux à partir des matrices de cooccurrence. L'étude menée montre l'avantage de ces statistiques par rapport à celles, d'ordre un, extraites des histogrammes de niveaux de gris. Le second point abordé dans cet article concerne la fusion. Elle est réalisée grâce à une modélisation par champs de Markov, à partir du graphe d'adjacence irrégulier de régions issues de la division. Des contributions sont amenées afin d'estimer les hyper-paramètres du système et le nombre d'étiquettes. Plusieurs résultats de segmentation sur différents types d'images réelles sont présentés afin de valider la méthode. Une étude comparative sur les différentes stratégies d'utilisation de relaxation par champs de Markov est alors menée, de manière rigoureuse, sur des images de synthèse. Cette comparaison est effectuée aussi bien du point de vue qualité des résultats que du point de vue coût algorithmique. Elle permet de montrer les avantages de la méthode proposée : diminuer considérablement les temps de calcul mis pour obtenir les segmentations, tout en n'altérant pas la qualité des résultats de celles-ci

Unsupervised segmentation of road images. A multicriteria approach

This paper presents a region-based segmentation algorithm which can be applied to various problems since it does not requir e a priori knowledge concerning the kind of processed images . This algorithm, based on a split and merge method, gives reliable results both on homogeneous grey level images and on textured images . First, images are divided into rectangular sectors . The splitting algorithm works independently on each sector, and uses a homogeneity criterion based only on grey levels . The mergin g is then achieved through assigning labels to each region obtained by the splitting step, using extracted feature measurements . We modeled exploited fields (data field and label field) by Markov Random Fields (MRF), the segmentation is then optimall y determined using the Iterated Conditional Modes (ICM) . Input data of the merging step are regions obtained by the splitting step and their corresponding features vector. The originality of this algorithm is that texture coefficients are directly computed from these regions . These regions will be elementary sites for the Markov relaxation process . Thus, a region- based segmentation algorith m using texture and grey level is obtained . Results from various images types are presented .Nous présentons ici un algorithme de segmentation en régions pouvant s'appliquer à des problèmes très variés car il ne tient compte d'aucune information a priori sur le type d'images traitées. Il donne de bons résultats aussi bien sur des images possédant des objets homogènes au sens des niveaux de gris que sur des images possédant des régions texturées. C'est un algorithme de type division-fusion. Lors d'une première étape, l'image est découpée en fenêtres, selon une grille. L'algorithme de division travaille alors indépendamment sur chaque fenêtre, et utilise un critère d'homogénéité basé uniquement sur les niveaux de gris. La texture de chacune des régions ainsi obtenues est alors calculée. A chaque région sera associé un vecteur de caractéristiques comprenant des paramètres de luminance, et des paramètres de texture. Les régions ainsi définies jouent alors le rôle de sites élémentaires pour le processus de fusion. Celui-ci est fondé sur la modélisation des champs exploités (champ d'observations et champ d'étiquettes) par des champs de Markov. Nous montrerons les résultats de segmentation obtenus sur divers types d'images

Two distinct STLV-1 subtypes infecting Mandrillus sphinx follow the geographic distribution of their hosts

The mandrill (Mandrillus sphinx) has been shown to be infected with an STLV-1 closely related to HTLV-1. Two distinct STLV-1 subtypes (D and F) infect wild mandrills with high overall prevalence (27.0%) but are different with respect to their phylogenetic relationship and parallel to the mandrills' geographic range. The clustering of these new STLV-1mnd sequences with HTLV-1 subtype D and F suggests first, past simian-tohuman transmissions in Central Africa and second, that species barriers are easier to cross over than geographic barriers

Dead or alive: animal sampling during Ebola hemorrhagic fever outbreaks in humans

There are currently no widely accepted animal surveillance guidelines for human Ebola hemorrhagic fever (EHF) outbreak investigations to identify potential sources of Ebolavirus (EBOV) spillover into humans and other animals. Animal field surveillance during and following an outbreak has several purposes, from helping identify the specific animal source of a human case to guiding control activities by describing the spatial and temporal distribution of wild circulating EBOV, informing public health efforts, and contributing to broader EHF research questions. Since 1976, researchers have sampled over 10,000 individual vertebrates from areas associated with human EHF outbreaks and tested for EBOV or antibodies. Using field surveillance data associated with EHF outbreaks, this review provides guidance on animal sampling for resource-limited outbreak situations, target species, and in some cases which diagnostics should be prioritized to rapidly assess the presence of EBOV in animal reservoirs. In brief, EBOV detection was 32.7% (18/55) for carcasses (animals found dead) and 0.2% (13/5309) for live captured animals. Our review indicates that for the purposes of identifying potential sources of transmission from animals to humans and isolating suspected virus in an animal in outbreak situations, (1) surveillance of free-ranging non-human primate mortality and morbidity should be a priority, (2) any wildlife morbidity or mortality events should be investigated and may hold the most promise for locating virus or viral genome sequences, (3) surveillance of some bat species is worthwhile to isolate and detect evidence of exposure, and (4) morbidity, mortality, and serology studies of domestic animals should prioritize dogs and pigs and include testing for virus and previous exposure

Cross-Dehydrogenative Couplings between Indoles and β-Keto Esters : Ligand-Assisted Ligand Tautomerization and Dehydrogenation via a Proton-Assisted Electron Transfer to Pd(II)

Cross-dehydrogenative coupling reactions between -ketoesters and electron-rich arenes, such as indoles, proceed with high regiochemical fidelity with a range of -ketoesters and indoles. The mechanism of the reaction between a prototypical -ketoester, ethyl 2-oxocyclopentanonecarboxylate and N-methylindole, has been studied experimentally by monitoring the temporal course of the reaction by 1H NMR, kinetic isotope effect studies, and control experiments. DFT calculations have been carried out using a dispersion-corrected range-separated hybrid functional (B97X-D) to explore the basic elementary steps of the catalytic cycle. The experimental results indicate that the reaction proceeds via two catalytic cycles. Cycle A, the dehydrogenation cycle, produces an enone intermediate. The dehydrogenation is assisted by N-methylindole, which acts as a ligand for Pd(II). The compu-tational studies agree with this conclusion, and identify the turnover-limiting step of the dehydrogenation step, which involves a change in the coordination mode of the -keto ester ligand from an O,O’-chelate to an C-bound Pd enolate. This ligand tautom-erization event is assisted by the -bound indole ligand. Subsequent scission of the ’-C–H bond takes place via a proton-assisted electron transfer mechanism, where Pd(II) acts as an electron sink and the trifluoroacetate ligand acts as a proton acceptor, to pro-duce the Pd(0) complex of the enone intermediate. The coupling is completed in cycle B, where the enone is coupled with indole. Pd(TFA)2 and TFA-catalyzed pathways were examined experimentally and computationally for this cycle, and both were found to be viable routes for the coupling step

Effect of Fructooligosaccharide Metabolism on Chicken Colonization by an Extra-Intestinal Pathogenic Escherichia coli Strain

Extra-intestinal pathogenic Escherichia coli (ExPEC) strains cause many diseases in humans and animals. While remaining asymptomatic, they can colonize the intestine for subsequent extra-intestinal infection and dissemination in the environment. We have previously identified the fos locus, a gene cluster within a pathogenicity island of the avian ExPEC strain BEN2908, involved in the metabolism of short-chain fructooligosaccharides (scFOS). It is assumed that these sugars are metabolized by the probiotic bacteria of the microbiota present in the intestine, leading to a decrease in the pathogenic bacterial population. However, we have previously shown that scFOS metabolism helps BEN2908 to colonize the intestine, its reservoir. As the fos locus is located on a pathogenicity island, one aim of this study was to investigate a possible role of this locus in the virulence of the strain for chicken. We thus analysed fos gene expression in extracts of target organs of avian colibacillosis and performed a virulence assay in chickens. Moreover, in order to understand the involvement of the fos locus in intestinal colonization, we monitored the expression of fos genes and their implication in the growth ability of the strain in intestinal extracts of chicken. We also performed intestinal colonization assays in axenic and Specific Pathogen-Free (SPF) chickens. We demonstrated that the fos locus is not involved in the virulence of BEN2908 for chickens and is strongly involved in axenic chicken cecal colonization both in vitro and in vivo. However, even if the presence of a microbiota does not inhibit the growth advantage of BEN2908 in ceca in vitro, overall, growth of the strain is not favoured in the ceca of SPF chickens. These findings indicate that scFOS metabolism by an ExPEC strain can contribute to its fitness in ceca but this benefit is fully dependent on the bacteria present in the microbiota