Silica coatings in the Ka’u Desert, Hawaii, a Mars analog terrain: A micromorphological, spectral, chemical, and isotopic study

High-silica materials have been observed on Mars, both from orbit by the CRISM spectrometer and in situ by the Spirit rover at Gusev Crater. These observations potentially imply a wet, geologically active Martian surface. To understand silica formation on Mars, it is useful to study analogous terrestrial silica deposits. We studied silica coatings that occur on the 1974 Kilauea flow in the Ka'u Desert, Hawaii. These coatings are typically composed of two layers: a ~10 μm layer of amorphous silica, capped by a ~1 μm layer of Fe-Ti oxide. The oxide coating is composed of ~100 nm spherules, suggesting formation by chemical deposition. Raman spectroscopy indicates altered silica glass as the dominant phase in the silica coating and anatase and rutile as dominant phases in the Fe-Ti coating; jarosite also occurs within the coatings. Oxygen isotopic contents of the coatings were determined by secondary ion mass spectrometry (Cameca 7f and NanoSIMS). The measured values, δ^(18)O_(Fe-Ti) = 14.6 ± 2.1‰, and δ^(18)O_(silica) = 12.1 ± 2.2‰ (relative to SMOW), are enriched in ^(18)O relative to the basalt substrate. The observations presented are consistent with a residual formation mechanism for the silica coating. Acid-sulfate solutions leached away divalent and trivalent cations, leaving a silica-enriched layer behind. Micrometer-scale dissolution and reprecipitation may have also occurred within the coatings. Chemical similarities between the Hawaiian samples and the high-silica deposits at Gusev suggest that the Martian deposits are the product of extended periods of similar acid-sulfate leaching

Human annexin A6 interacts with influenza a virus protein M2 and negatively modulates infection

Copyright © 2012, American Society for Microbiology. All Rights ReservedThe influenza A virus M2 ion channel protein has the longest cytoplasmic tail (CT) among the three viral envelope proteins and is well conserved between different viral strains. It is accessible to the host cellular machinery after fusion with the endosomal membrane and during the trafficking, assembly, and budding processes. We hypothesized that identification of host cellular interactants of M2 CT could help us to better understand the molecular mechanisms regulating the M2-dependent stages of the virus life cycle. Using yeast two-hybrid screening with M2 CT as bait, a novel interaction with the human annexin A6 (AnxA6) protein was identified, and their physical interaction was confirmed by coimmunoprecipitation assay and a colocalization study of virus-infected human cells. We found that small interfering RNA (siRNA)-mediated knockdown of AnxA6 expression significantly increased virus production, while its overexpression could reduce the titer of virus progeny, suggesting a negative regulatory role for AnxA6 during influenza A virus infection. Further characterization revealed that AnxA6 depletion or overexpression had no effect on the early stages of the virus life cycle or on viral RNA replication but impaired the release of progeny virus, as suggested by delayed or defective budding events observed at the plasma membrane of virus-infected cells by transmission electron microscopy. Collectively, this work identifies AnxA6 as a novel cellular regulator that targets and impairs the virus budding and release stages of the influenza A virus life cycle.This work was supported by the Research Fund for the Control of Infectious Disease (project 09080892) of the Hong Kong Government, the Area of Excellence Scheme of the University Grants Committee (grant AoE/M-12/-06 of the Hong Kong Special Administrative Region, China), the French Ministry of Health, the RESPARI Pasteur Network

Ambient and cold-temperature infrared spectra and XRD patterns of ammoniated phyllosilicates and carbonaceous chondrite meteorites relevant to Ceres and other solar system bodies

Mg‐phyllosilicate‐bearing, dark surface materials on the dwarf planet Ceres have NH_4‐bearing materials, indicated by a distinctive 3.06 μm absorption feature. To constrain the identity of the Ceres NH_4‐carrier phase(s), we ammoniated ground particulates of candidate materials to compare their spectral properties to infrared data acquired by Dawn's Visible and Infrared (VIR) imaging spectrometer. We treated Mg‐, Fe‐, and Al‐smectite clay minerals; Mg‐serpentines; Mg‐chlorite; and a suite of carbonaceous meteorites with NH_4‐acetate to exchange ammonium. Serpentines and chlorites showed no evidence for ammoniation, as expected due to their lack of exchangeable interlayer sites. Most smectites showed evidence for ammoniation by incorporation of NH_4^+ into their interlayers, resulting in the appearance of absorptions from 3.02 to 3.08 μm. Meteorite samples tested had weak absorptions between 3.0 and 3.1 μm but showed little clear evidence for enhancement upon ammoniation, likely due to the high proportion of serpentine and other minerals relative to expandable smectite phases or to NH_4^+ complexing with organics or other constituents. The wavelength position of the smectite NH4 absorption showed no variation between IR spectra acquired under dry‐air purge at 25 °C and under vacuum at 25 °C to −180 °C. Collectively, data from the smectite samples show that the precise center wavelength of the characteristic ~3.05 μm v_3 absorption in NH_4 is variable and is likely related to the degree of hydrogen bonding of NH_4‐H_2O complexes. Comparison with Dawn VIR spectra indicates that the hypothesis of Mg‐saponite as the ammonium carrier phase is the simplest explanation for observed data, and that Ceres dark materials may be like Cold Bokkeveld or Tagish Lake but with proportionally more Mg‐smectite

Silica coatings on the 1974 Kilauea flow: new SEM and SIMS results and implications for Mars

Despite the predominately mafic character of martian surface rocks, silica-rich materials have long been predicted to occur on Mars; recently, those predictions have been validated. CRISM spectra from numerous regions of Mars have revealed H_2O and OH-bearing phases most consistent with amorphous silica. Additionally, the detection of high-silica materials at Home Plate by MER Spirit implied aqueous alteration and leaching in a volcanic environment [3]. In order to fully understand the environments in which silica-rich materials are formed on Mars, it is useful to study silica in analogous terrestrial settings. We focus on silica and Fe-Ti oxide coatings in the Ka’u Desert on the island of Hawaii, an analog to Mars characterized by low levels of rainfall and strong acid-sulfate alteration processes [4]. Many formation mechanisms for these coatings have been proposed, including dissolution of wind-blown tephra [5], leaching of volcanic glass [6], and vapor deposition [7]. We focus on a suite of samples from the 1974 Kilauea pahoehoe flow, collected in 2003. The chemistry and morphology of these coatings were previously presented [8]. Here we present new morphological, spectral and isotopic analyses of the coating suite. The goal of the study is to characterize the coatings and their formation mechanism and describe the implications for silica mobility on Mars

Design of low impact development in the urban context considering hydrological performance and life-cycle cost

The pressures on water system are increasing in cities. Rapid urbanisation caused by booming population leads to more impervious area and less infiltration, with the consequence of larger runoff volume and higher flood risk. Launched in 2014, the low impact development (LID), an important part of Sponge City in China initiative, invests in projects that aim to restore the water cycle in the urban area. A comprehensive understanding of the performance of LID measures at watershed scale under different rainfall scenarios and life cycle costs is necessary. The objectives of this study are to assess the hydrological performance and to identify the optimal LID design by using SWMM model and life cycle cost (LCC) method. This study found that LID practices, including bioretention, grass swale, and permeable pavement, showed good performance on urban storm mitigation at watershed scale under different rainfall scenarios. Furthermore, the rates of surface runoff reduction were largely insusceptible to the change of rainfall volume and duration. Regarding the cost-effectiveness, the priority was grass swale > bioretention > permeable pavement in the study area. The optimal LID scenario was the combination of these three types of LID. The proposed approach can help the decision-makers to determine the preferable LID plan suitable for the local communities

Identification of HLA-DRPheβ47 as the susceptibility marker of hypersensitivity to beryllium in individuals lacking the berylliosis-associated supratypic marker HLA-DPGluβ69

BACKGROUND: Susceptibility to beryllium (Be)-hypersensitivity (BH) has been associated with HLA-DP alleles carrying a glutamate at position 69 of the HLA-DP β-chain (HLA-DPGlu69) and with several HLA-DP, -DQ and -DR alleles and polymorphisms. However, no genetic associations have been found between BH affected subjects not carrying the HLA-DPGlu69 susceptibility marker. METHODS: In this report, we re-evaluated an already described patient populations after 7 years of follow-up including new 29 identified BH subjects. An overall population 36 berylliosis patients and 38 Be-sensitization without lung granulomas and 86 Be-exposed controls was analysed to assess the role of the individual HLA-class II polymorphisms associated with BH-susceptibility in HLA-DPGlu69 negative subjects by univariate and multivariate analysis. RESULTS: As previously observed in this population the HLA-DPGlu69 markers was present in higher frequency in berylliosis patients (31 out of 36, 86%) than in Be-sensitized (21 out of 38, 55%, p = 0.008 vs berylliosis) and 41 out of 86 (48%, p < 0.0001 vs berylliosis, p = 0.55 vs Be-sensitized) Be-exposed controls. However, 22 subjects presenting BH did not carry the HLA-DPGlu69 marker. We thus evaluated the contribution of all the HLA-DR, -DP and -DQ polymorphisms in determining BH susceptibility in this subgroup of HLA-Glu69 subjects. In HLA-DPGlu69-negatives a significant association with BH was found for the HLA-DQLeu26, for the HLA-DRB1 locus residues Ser13, Tyr26, His32, Asn37, Phe47 and Arg74 and for the HLA-DRB3 locus clusterized residues Arg11, Tyr26, Asp28, Leu38, Ser60 and Arg74. HLA-DRPhe47 (OR 2.956, p < 0.05) resulting independently associated with BH. Further, Be-stimulated T-cell proliferation in the HLA-DPGlu69-negative subjects (all carrying HLA-DRPhe47) was inhibited by the anti-HLA-DR antibody (range 70–92% inhibition) significantly more than by the anti-HLA-DP antibody (range: 6–29%; p < 0.02 compared to anti-HLA-DR) while it was not affected by the anti-HLA-DQ antibody. CONCLUSION: We conclude that HLA-DPGlu69 is the primary marker of Be-hypersensitivity and HLA-DRPhe47 is associated with BH in Glu69-negative subjects, likely playing a role in Be-presentation and sensitization

Rho GTPase Transcriptome Analysis Reveals Oncogenic Roles for Rho GTPase-Activating Proteins in Basal-like Breast Cancers

The basal-like breast cancer (BLBC) subtype accounts for a disproportionately high percentage of overall breast cancer mortality. The current therapeutic options for BLBC need improvement; hence, elucidating signaling pathways that drive BLBC growth may identify novel targets for the development of effective therapies. Rho GTPases have previously been implicated in promoting tumor cell proliferation and metastasis. These proteins are inactivated by Rho-selective GTPase-activating proteins (RhoGAPs), which have generally been presumed to act as tumor suppressors. Surprisingly, RNA-Seq analysis of the Rho GTPase signaling transcriptome revealed high expression of several RhoGAP genes in BLBC tumors, raising the possibility that these genes may be oncogenic. To evaluate this, we examined the roles of two of these RhoGAPs, ArhGAP11A (also known as MP-GAP) and RacGAP1 (also known as MgcRacGAP), in promoting BLBC. Both proteins were highly expressed in human BLBC cell lines, and knockdown of either gene resulted in significant defects in the proliferation of these cells. Knockdown of ArhGAP11A caused CDKN1B/p27-mediated arrest in the G1 phase of the cell cycle, whereas depletion of RacGAP1 inhibited growth through the combined effects of cytokinesis failure, CDKN1A/p21-mediated RB1 inhibition, and the onset of senescence. Random migration was suppressed or enhanced by the knockdown of ArhGAP11A or RacGAP1, respectively. Cell spreading and levels of GTP-bound RhoA were increased upon depletion of either GAP. We have established that, via the suppression of RhoA, ArhGAP11A and RacGAP1 are both critical drivers of BLBC growth, and propose that RhoGAPs can act as oncogenes in cancer

Cryotomography of budding influenza a virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end

Influenza viruses exhibit striking variations in particle morphology between strains. Clinical isolates of influenza A virus have been shown to produce long filamentous particles while laboratory-adapted strains are predominantly spherical. However, the role of the filamentous phenotype in the influenza virus infectious cycle remains undetermined. We used cryo-electron tomography to conduct the first three-dimensional study of filamentous virus ultrastructure in particles budding from infected cells. Filaments were often longer than 10 microns and sometimes had bulbous heads at their leading ends, some of which contained tubules we attribute to M1 while none had recognisable ribonucleoprotein (RNP) and hence genome segments. Long filaments that did not have bulbs were infrequently seen to bear an ordered complement of RNPs at their distal ends. Imaging of purified virus also revealed diverse filament morphologies; short rods (bacilliform virions) and longer filaments. Bacilliform virions contained an ordered complement of RNPs while longer filamentous particles were narrower and mostly appeared to lack this feature, but often contained fibrillar material along their entire length. The important ultrastructural differences between these diverse classes of particles raise the possibility of distinct morphogenetic pathways and functions during the infectious process

Building social capital through breastfeeding peer support: Insights from an evaluation of a voluntary breastfeeding peer support service in North-West England

Background: Peer support is reported to be a key method to help build social capital in communities. To date there are no studies that describe how this can be achieved through a breastfeeding peer support service. In this paper we present findings from an evaluation of a voluntary model of breastfeeding peer support in North-West England to describe how the service was operationalized and embedded into the community. This study was undertaken from May, 2012 to May, 2013. Methods: Interviews (group or individual) were held with 87 participants: 24 breastfeeding women, 13 peer supporters and 50 health and community professionals. The data contained within 23 monthly monitoring reports (January, 2011 to February 2013) compiled by the voluntary peer support service were also extracted and analysed. Results: Thematic analysis was undertaken using social capital concepts as a theoretical lens. Key findings were identified to resonate with ’bonding’, ‘bridging’ and ‘linking’ forms of social capital. These insights illuminate how the peer support service facilitates ‘bonds’ with its members, and within and between women who access the service; how the service ‘bridges’ with individuals from different interests and backgrounds, and how ‘links’ were forged with those in authority to gain access and reach to women and to promote a breastfeeding culture. Some of the tensions highlighted within the social capital literature were also identified. Conclusions: Horizontal and vertical relationships forged between the peer support service and community members enabled peer support to be embedded into care pathways, helped to promote positive attitudes to breastfeeding and to disseminate knowledge and maximise reach for breastfeeding support across the community. Further effort to engage with those of different ethnic backgrounds and to resolve tensions between peer supporters and health professionals is warranted