Variational Sequences, Representation Sequences and Applications in Physics

This paper is a review containing new original results on the finite order variational sequence and its different representations with emphasis on applications in the theory of variational symmetries and conservation laws in physics

Wind and nebula of the M33 variable GR290 (WR/LBV)

Context: GR290 (M33/V532=Romano's Star) is a suspected post-LBV star located in M33 galaxy that shows a rare Wolf-Rayet spectrum during its minimum light phase. In spite of many studies, its atmospheric structure, its circumstellar environment and its place in the general context of massive stars evolution is poorly known. Aims: Detailed study of its wind and mass loss, and study of the circumstellar environment associated to the star. Methods: Long-slit spectra of GR290 were obtained during its present minimum luminosity phase with the GTC together with contemporaneous BVRI photometry. The data were compared with non-LTE model atmosphere synthetic spectra computed with CMFGEN and with CLOUDY models for ionized interstellar medium regions. Results: The current $m_V=18.8$ mag, is the faintest at which this source has ever been observed. The non-LTE models indicate effective temperature $T_{eff}$=27-30 kK at radius $R_{2/3}$=27-21 Rsun and mass loss rate $\dot{M}=1.5\times10^{-5}$ Msun yr$^{-1}$. The terminal wind speed $V_\infty$=620 ${\rm km~s^{-1}}$ is faster than ever before recorded while the current luminosity $L_*=(3.1-3.7)\times 10^5$ Lsun is the lowest ever deduced. It is overabundant in He and N and underabundant in C and O. It is surrounded by an unresolved compact HII region with dimensions $\leq$4 pc, from where H-Balmer, HeI lines and [OIII] and [NII] are detected. In addition, we find emission from a more extended interstellar medium (ISM) region which appears to be asymmetric, with a larger extent to the East (16-40 pc) than to the West. Conclusions: In the present long lasting visual minimum, GR290 is in a lower bolometric luminosity state with higher mass loss rate. The nearby nebular emission seems to suggest that the star has undergone significant mass loss over the past $10^4-10^5$ years and is nearing the end stages of its evolution.Comment: submitted to A&A, 12 pages, 9 figures, 7 table

Machine translation for language learners

Machine Translation (MT) has been controversial in second and foreign language learning, but the strategic integration of MT might be beneficial to language learning in certain contexts. In this chapter we discuss the conditions in which MT can be useful in language learning, set out digital alternatives to MT, and provide examples of how MT can support language learners

Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis

Circular RNAs (circRNAs) constitute a family of transcripts with unique structures and still largely unknown functions. Their biogenesis, which proceeds via a back-splicing reaction, is fairly well characterized, whereas their role in the modulation of physiologically relevant processes is still unclear. Here we performed expression profiling of circRNAs during in vitro differentiation of murine and human myoblasts, and we identified conserved species regulated in myogenesis and altered in Duchenne muscular dystrophy. A high-content functional genomic screen allowed the study of their functional role in muscle differentiation. One of them, circ-ZNF609, resulted in specifically controlling myoblast proliferation. Circ-ZNF609 contains an open reading frame spanning from the start codon, in common with the linear transcript, and terminating at an in-frame STOP codon, created upon circularization. Circ-ZNF609 is associated with heavy polysomes, and it is translated into a protein in a splicing-dependent and cap-independent manner, providing an example of a protein-coding circRNA in eukaryotes

Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP)

The study was aimed at identifying endogenous proteins which assist or impede the permeabilized state in the cell membrane disrupted by nsEP (20 or 40 pulses, 300 ns width, 7 kV/cm). We employed a LentiArray CRISPR library to generate knockouts (KOs) of 316 genes encoding for membrane proteins in U937 human monocytes stably expressing Cas9 nuclease. The extent of membrane permeabilization by nsEP was measured by the uptake of Yo-Pro-1 (YP) dye and compared to sham-exposed KOs and control cells transduced with a non-targeting (scrambled) gRNA. Only two KOs, for SCNN1A and CLCA1 genes, showed a statistically significant reduction in YP uptake. The respective proteins could be part of electropermeabilization lesions or increase their lifespan. In contrast, as many as 39 genes were identified as likely hits for the increased YP uptake, meaning that the respective proteins contributed to membrane stability or repair after nsEP. The expression level of eight genes in different types of human cells showed strong correlation (R \u3e 0.9, p \u3c 0.02) with their LD50 for lethal nsEP treatments, and could potentially be used as a criterion for the selectivity and efficiency of hyperplasia ablations with nsEP

Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied by Immunogenic Cell Death in Murine Models of Lymphoma and Colorectal Cancer

Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the trafficking of danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality for tumor ablation, cause the activation of the ER-resident stress sensor PERK in both CT-26 colon carcinoma and EL-4 lymphoma cells. PERK activation correlates with sustained CRT exposure on the cell plasma membrane and apoptosis induction in both nsPEF-treated cell lines. Our results show that, in CT-26 cells, the activity of caspase-3/7 was increased fourteen-fold as compared with four-fold in EL-4 cells. Moreover, while nsPEF treatments induced the release of the ICD hallmark HMGB1 in both cell lines, extracellular ATP was detected only in CT-26. Finally, in vaccination assays, CT-26 cells treated with nsPEF or doxorubicin equally impaired the growth of tumors at challenge sites eliciting a protective anticancer immune response in 78% and 80% of the animals, respectively. As compared to CT-26, both nsPEF- and mitoxantrone-treated EL-4 cells had a less pronounced effect and protected 50% and 20% of the animals, respectively. These results support our conclusion that nsPEF induce ER stress, accompanied by bona fide ICD