221 research outputs found
Phytochrome-Mediated Light Perception Affects Fruit Development and Ripening Through Epigenetic Mechanisms
Phytochrome (PHY)-mediated light and temperature perception has been increasingly implicated as important regulator of fruit development, ripening, and nutritional quality. Fruit ripening is also critically regulated by chromatin remodeling via DNA demethylation, though the molecular basis connecting epigenetic modifications in fruits and environmental cues remains largely unknown. Here, to unravel whether the PHY-dependent regulation of fruit development involves epigenetic mechanisms, an integrative analysis of the methylome, transcriptome and sRNAome of tomato fruits from phyA single and phyB1B2 double mutants was performed in immature green (IG) and breaker (BK) stages. The transcriptome analysis showed that PHY-mediated light perception regulates more genes in BK than in the early stages of fruit development (IG) and that PHYB1B2 has a more substantial impact than PHYA in the fruit transcriptome, in both analyzed stages. The global profile of methylated cytosines revealed that both PHYA and PHYB1B2 affect the global methylome, but PHYB1B2 has a greater impact on ripening-associated methylation reprogramming across gene-rich genomic regions in tomato fruits. Remarkably, promoters of master ripening-associated transcription factors (TF) (RIN, NOR, CNR, and AP2a) and key carotenoid biosynthetic genes (PSY1, PDS, ZISO, and ZDS) remained highly methylated in phyB1B2 from the IG to BK stage. The positional distribution and enrichment of TF binding sites were analyzed over the promoter region of the phyB1B2 DEGs, exposing an overrepresentation of binding sites for RIN as well as the PHY-downstream effectors PIFs and HY5/HYH. Moreover, phyA and phyB1B2 mutants showed a positive correlation between the methylation level of sRNA cluster-targeted genome regions in gene bodies and mRNA levels. The experimental evidence indicates that PHYB1B2 signal transduction is mediated by a gene expression network involving chromatin organization factors (DNA methylases/demethylases, histone-modifying enzymes, and remodeling factors) and transcriptional regulators leading to altered mRNA profile of ripening-associated genes. This new level of understanding provides insights into the orchestration of epigenetic mechanisms in response to environmental cues affecting agronomical traits.Fil: Bianchetti, Ricardo. Universidade de Sao Paulo; BrasilFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Haro, Luis Alejandro. Weizmann Institute Of Science Israel; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zuccarelli, Rafael. Universidade de Sao Paulo; BrasilFil: Rosado, Daniele. Universidade de Sao Paulo; BrasilFil: Freschi, Luciano. Universidade de Sao Paulo; BrasilFil: Rossi, Magdalena. Universidade de Sao Paulo; BrasilFil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentin
Plant degreening: evolution and expression of tomato\ud (Solanum lycopersicum) dephytylation enzymes
Chlorophyll is the most abundant pigment on earth and even though it is known that its high photo-excitability necessitates a tight regulation of its degradation pathway, to date there are still several steps in chlorophyll breakdown that remain obscure. In order to better understand the ‘degreening’ processes that accompany leaf senescence and fruit ripening, we characterized the enzyme-encoding genes involved in dephytylation from tomato (Solanum lycopersicum). A single pheophytinase (PPH) gene and four chlorophyllase (CLH) genes were identified in the tomato genome. A phenetic analysis revealed two groups of CLHs in eudicot species and further evolutionary analysis indicated that these enzymes are under diverse selection pressures. A comprehensive expression profile analysis also suggested functional specificity for these dephytylating enzymes. The integrated analysis allows us to propose three general roles for chlorophyll dephytylation: i) PPH, which is under high selective constraint, is responsible for chlorophyll degradation during developmentally programed physiological processes; ii) Group I CLHs, which are under relaxed selection constraint, respond to environmental and hormonal stimuli and play a role in plant adaptation plasticity; and iii) Group II CLHs, which are also under high selective constraint, are mostly involved in chlorophyll recycling.BSL and JA were recipients of FAPESP fellowships and MR was funded by a fellowship from CNPq. This work was supported by FAPESP2012-12531-8 (Brazil). The authors thank Rohm and Haas Company for generous donation of the 1-methylcyclopropene (SmartFresh®) used for the experiments
Consequences of haemogregarine infection on the escape distance in the lacertid lizard, Podarcis vaucheri
Nowadays it is widely accepted that parasites play a significant role in the community structures in which they occur, and ultimately upon ecosystems. Furthermore, infection by parasites might be associated with considerable deterioration of individual host fitness. While the apicomplexan parasites belonging to the genus Hepatozoon can provoke severe deleterious effects in some mammals, impact on other hosts, such as reptiles, is still unclear. We assessed the effect of Hepatozoon parasites on Podarcis vaucheri flight-initiation distance from a simulated predator, a behaviour that is determinant for a successful escape and is therefore likely to have major implications on a lizard’s survival. We found that flight-initiation distance was not dependent on the time of the day or tail condition. Subadults exhibited worse body condition than adults and females had worse body condition than males. Regarding intensity of parasitism, subadults showed higher parasitemia levels. Escape distance was not associated with parasitic load or any of the other studied features, which is indicative of limited impact of the parasite. This negligible effect might explain the remarkably high prevalence (more than 96%) of this parasitic group within this P. vaucheri population
Registro da avaliação de enfermagem em terapia intensiva: Discurso do Sujeito Coletivo
Aim: to understand the perceptions of nurses about the records of the nursing process in a intensive care unit. Method: descriptive qualitative study, developed with nine nurses in a public hospital located in a county in southern Brazil. Data were collected through semi-structured interview script and the analysis was performed through the Collective Subject Discourse. Results: they showed the use of only parts of the nursing process, its strengths and weaknesses. The practicality and quickness to implement the records are considered positive aspects and the failure of other evaluation criteria, and the shallowness of information, such as negative. Final remarks: incomplete utilization of nursing process remains a difficulty for nurses. There is need for proposals that allow nurses to apply new approaches in practice, helping to qualify the records, promoting patient safetyObjetivo: conocer las percepciones de las enfermeras sobre el proceso de evaluación de enfermería y su registro en una Unidad de Cuidados Intensivos. Método: estudio cualitativo descriptivo, desarrollado con nueve enfermeras de un hospital público en Brasil. Los datos fueron recolectados a través de entrevista y el análisis realizado por medio de la técnica del Discurso del Sujeto Colectivo. Resultados: demostraron el uso de sólo algunas partes del proceso de enfermería, sus potencialidades y debilidades. La practicidad y rapidez para poner en práctica los documentos son los aspectos positivos y el fracaso de los otros criterios de evaluación y la poca profundidad de la información, negativos. Consideraciones finales: el uso incompleto del Proceso de Enfermería sigue siendo una dificultad para las enfermeras. Existe la necesidad de propuestas que permitan a las enfermeras nuevos enfoques en la práctica, ayudando a calificar los registros y promoviendo de la seguridad del paciente.http://dx.doi.org/10.5902/2179769215509Objetivo: compreender as percepções de enfermeiros sobre a avaliação de enfermagem e seu registro em uma Unidade de Terapia Intensiva. Método: trata-se de um estudo descritivo de caráter qualitativo, desenvolvido com nove enfermeiros de um hospital público, situado em um município do sul do Brasil. Os dados foram coletados mediante roteiro de entrevista semiestruturado e a análise realizada por meio do Discurso do Sujeito Coletivo. Resultados: apontaram a utilização de apenas partes do processo de enfermagem, suas potencialidades e fragilidades. A praticidade e a rapidez para a execução dos registros são aspectos considerados positivos e a insuficiência de outros critérios de avaliação e a superficialidade das informações, como negativos. Considerações Finais: a utilização incompleta do Processo de Enfermagem permanece uma dificuldade para enfermeiros. Há necessidade de propostas aplicáveis que permitam aos enfermeiros novas abordagens na prática, contribuindo para qualificar os registros, favorecendo a segurança o paciente
Pheophytinase knockdown impacts carbon metabolism and nutraceutical content under normal growth conditions in tomato
Although chlorophyll (Chl) degradation is an essential biochemical pathway for plant physiology, our knowledge regarding this process still has unfilled gaps. Pheophytinase (PPH) was shown to be essential for Chl breakdown in dark-induced senescent leaves. However, the catalyzing enzymes involved in pigment turnover and fruit ripening-associated degreening are still controversial. Chl metabolism is closely linked to the biosynthesis of other isoprenoid-derived compounds, such as carotenoids and tocopherols, which are also components of the photosynthetic machinery. Chls, carotenoids and tocopherols share a common precursor, geranylgeranyl diphosphate, produced by the plastidial methylerythritol 4-phosphate (MEP) pathway. Additionally, the Chl degradation-derived phytol can be incorporated into tocopherol biosynthesis. In this context, tomato turns out to be an interesting model to address isoprenoid-metabolic cross-talk since fruit ripening combines degreening and an intensely active MEP leading to carotenoid accumulation. Here, we investigate the impact of PPH deficiency beyond senescence by the comprehensive phenotyping of SlPPH-knockdown tomato plants. In leaves, photosynthetic parameters indicate altered energy usage of excited Chl. As a mitigatory effect, photosynthesis-associated carotenoids increased while tocopherol content remained constant. Additionally, starch and soluble sugar profiles revealed a distinct pattern of carbon allocation in leaves that suggests enhanced sucrose exportation. The higher levels of carbohydrates in sink organs down-regulated carotenoid biosynthesis. Additionally, the reduction in Chl-derived phytol recycling resulted in decreased tocopherol content in transgenic ripe fruits. Summing up, tocopherol and carotenoid metabolism, together with the antioxidant capacity of the hydrophilic and hydrophobic fractions, were differentially affected in leaves and fruits of the transgenic plants. Thus, in tomato, PPH plays a role beyond senescence-associated Chl degradation that, when compromised, affects isoprenoid and carbon metabolism which ultimately alters the fruit's nutraceutical content
Oligodendrocyte precursor cells engulf synapses during circuit remodeling in mice
Oligodendrocyte precursor cells (OPCs) give rise to myelinating oligodendrocytes throughout life, but the functions of OPCs are not limited to oligodendrogenesis. Here we show that OPCs contribute to thalamocortical presynapse elimination in the developing and adult mouse visual cortex. OPC-mediated synapse engulfment increases in response to sensory experience during neural circuit refinement. Our data suggest that OPCs may regulate synaptic connectivity in the brain independently of oligodendrogenesis
Measurement of the cosmic ray spectrum above eV using inclined events detected with the Pierre Auger Observatory
A measurement of the cosmic-ray spectrum for energies exceeding
eV is presented, which is based on the analysis of showers
with zenith angles greater than detected with the Pierre Auger
Observatory between 1 January 2004 and 31 December 2013. The measured spectrum
confirms a flux suppression at the highest energies. Above
eV, the "ankle", the flux can be described by a power law with
index followed by
a smooth suppression region. For the energy () at which the
spectral flux has fallen to one-half of its extrapolated value in the absence
of suppression, we find
eV.Comment: Replaced with published version. Added journal reference and DO
Energy Estimation of Cosmic Rays with the Engineering Radio Array of the Pierre Auger Observatory
The Auger Engineering Radio Array (AERA) is part of the Pierre Auger
Observatory and is used to detect the radio emission of cosmic-ray air showers.
These observations are compared to the data of the surface detector stations of
the Observatory, which provide well-calibrated information on the cosmic-ray
energies and arrival directions. The response of the radio stations in the 30
to 80 MHz regime has been thoroughly calibrated to enable the reconstruction of
the incoming electric field. For the latter, the energy deposit per area is
determined from the radio pulses at each observer position and is interpolated
using a two-dimensional function that takes into account signal asymmetries due
to interference between the geomagnetic and charge-excess emission components.
The spatial integral over the signal distribution gives a direct measurement of
the energy transferred from the primary cosmic ray into radio emission in the
AERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air
shower arriving perpendicularly to the geomagnetic field. This radiation energy
-- corrected for geometrical effects -- is used as a cosmic-ray energy
estimator. Performing an absolute energy calibration against the
surface-detector information, we observe that this radio-energy estimator
scales quadratically with the cosmic-ray energy as expected for coherent
emission. We find an energy resolution of the radio reconstruction of 22% for
the data set and 17% for a high-quality subset containing only events with at
least five radio stations with signal.Comment: Replaced with published version. Added journal reference and DO
Measurement of the Radiation Energy in the Radio Signal of Extensive Air Showers as a Universal Estimator of Cosmic-Ray Energy
We measure the energy emitted by extensive air showers in the form of radio
emission in the frequency range from 30 to 80 MHz. Exploiting the accurate
energy scale of the Pierre Auger Observatory, we obtain a radiation energy of
15.8 \pm 0.7 (stat) \pm 6.7 (sys) MeV for cosmic rays with an energy of 1 EeV
arriving perpendicularly to a geomagnetic field of 0.24 G, scaling
quadratically with the cosmic-ray energy. A comparison with predictions from
state-of-the-art first-principle calculations shows agreement with our
measurement. The radiation energy provides direct access to the calorimetric
energy in the electromagnetic cascade of extensive air showers. Comparison with
our result thus allows the direct calibration of any cosmic-ray radio detector
against the well-established energy scale of the Pierre Auger Observatory.Comment: Replaced with published version. Added journal reference and DOI.
Supplemental material in the ancillary file
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