Validation of an indirect in-house ELISA using synthetic peptides to detect antibodies anti-gp90 and gp45 of the equine infectious anaemia virus

Background: Equine infectious anaemia (EIA) is controlled by the identification of seropositive animals. The official diagnostic method is the agar gel immunodiffusion (AGID) test, which detects antibodies against a viral core protein (p26). Although AGID is inexpensive and specific, the report of results takes considerable time and the test has low analytical sensitivity. Objective: To validate our in-house indirect ELISAgp90/45, following the World Organization of Animal Health (OIE) criteria. Study design: Test validation. Methods: Synthetic peptides gp90 and gp45 were used as antigens in ELISAgp90/45. Tests used for validation, calibration and linear working operating range, analytical and diagnostic sensitivity and specificity, repeatability and reproducibility were assessed by comparing them with the AGID test and using 1844 equine sera grouped into five different panels. Results: We were able to replace the National References Sera with our Internal Reference Sera. ELISAgp90/45 had acceptable repeatability and reproducibility. Analytical sensitivity of the ELISAgp90/45 was 800 times greater than that of AGID test for positive sera and 400 times greater for weak positive sera. ELISAgp90/45 also showed optimal analytical specificity, since no cross-reactivity was detected with antibodies against other equine viruses. One sample was positive by AGID test and negative by ELISAgp90/45. ELISAgp90/45 was performed using 243 EIA positive and 878 negative equid sera, and showed a diagnostic sensitivity of 99.59% [CI 97.73%-99.99%] and a diagnostic specificity of 90.32% [CI 88.17%-92.19%], compared to AGID test; thus, it was demonstrated to be a robust test. Main limitations: Samples were derived from naturally infected equid populations showing heterogeneous clinical states: therefore, their status was uncertain and some horses were sampled more than once. The AGID test may not be the most useful gold standard. Conclusion: ELISAgp90/45 is a useful tool for the diagnosis of EIAV infection and meets validation requirements established by the OIE.Fil: Russi, Romina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Inmunologia Experimental.; Argentina. Gobierno de la Provincia de Santa Fe. Ministerio de la Producción, Ciencia y Tecnología. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; ArgentinaFil: García, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Gobierno de la Provincia de Santa Fe. Ministerio de la Producción, Ciencia y Tecnología. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; ArgentinaFil: Cámara, María Silvia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Soutullo, Adriana Rosa. Gobierno de la Provincia de Santa Fe. Ministerio de la Producción, Ciencia y Tecnología. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Inmunologia Experimental.; Argentin

Magnetic resonance imaging as a predictor of therapeutic response to pasireotide in acromegaly

Acromegaly; Magnetic resonance imaging; PasireotideAcromegàlia; Imatges per ressonància magnètica; PasireòtidaAcromegalia; Imagen por resonancia magnética; PasireotidaObjective Hyperintensity signal in T2-weighted magnetic resonance imaging (MRI) has been related to better therapeutic response during pasireotide treatment in acromegaly. The aim of the study was to evaluate T2 MRI signal intensity and its relation with pasireotide therapeutic effectiveness in real-life clinical practice. Design, Patients and Measurements Retrospective multicentre study including acromegaly patients treated with pasireotide. Adenoma T2-weighted MRI signal at diagnosis was qualitatively classified as iso-hyperintense or hypointense. Insulin-like growth factor (IGF-I), growth hormone (GH) and tumour volume reduction were assessed after 6 and 12 months of treatment and its effectiveness evaluated according to baseline MRI signal. Hormonal response was considered ‘complete’ when normalization of IGF-I levels was achieved. Significant tumour shrinkage was defined as a volume reduction of ≥25% from baseline. Results Eighty-one patients were included (48% women, 50 ± 1.5 years); 93% had previously received somatostatin receptor ligands (SRLs) treatment. MRI signal was hypointense in 25 (31%) and hyperintense in 56 (69%) cases. At 12 months of follow-up, 42/73 cases (58%) showed normalization of IGF-I and 37% both GH and IGF-I. MRI signal intensity was not associated with hormonal control. 19/51 cases (37%) presented a significant tumour volume shrinkage, 16 (41%) from the hyperintense group and 3 (25%) from the hypointense. Conclusions T2-signal hyperintensity was more frequently observed in pasireotide treated patients. Almost 60% of SRLs resistant patients showed a complete normalization of IGF-I after 1 year of pasireotide treatment, regardless of the MRI signal. There was also no difference in the percentage tumour shrinkage over basal residual volume between the two groups.This work was partially supported by grants from Instituto de Salud Carlos III, Madrid, (PMP 15/00027, PMP22/00021 and PI22/01364) to M. P-D

Chemical characteristics of soybean meals available in the European Union market: A 2015 survey

The aim of this research was to determine the chemical composition and nutritive value of soybean meals (SBM) from beans of different origins collected in the European Union in 2015 . Based on a previous survey conducted from 2008 to 2014, we hypothesized that the SBM from the different countries could show differences in chemical composition, protein quality, and nutritional value. In total, 40 SBM samples from USA (n = 14), Brazil (BRA; n = 15), and Argentina (ARG; n = 11) were collected at random from 5 key feed compounders and analyzed for proximal components, minerals, sugars, fibers, amino acids (AA), and protein quality

Comparação da saúde subjetiva entre protótipos de personalidade em população geral do México

El objetivo de este estudio fue obtener prototipos de personalidad en población general de México y comparar la salud subjetiva entre ellos. En total, participaron 994 individuos de 14 a 63 años de edad. Se evaluaron cinco rasgos de personalidad (Neuroticismo, Extraversión, Apertura, Responsabilidad y Amabilidad) con el NEO-FFI, y la salud subjetiva con el GHQ-12 y una pregunta sobre el estado de salud. Para corroborar la consistencia de los prototipos se dividió la muestra en dos grupos de edad: joven (de 14 a 25 años) y maduro (de 26 a 63 años). Y como resultado se recuperaron tres prototipos en ambos grupos: las personas Resilientes -bajo neuroticismo y alto en el resto de los rasgos-, quienes tuvieron la mejor salud subjetiva; las No-Resilientes -alto neuroticismo y bajo en el resto de los rasgos-, que presentaron la peor salud subjetiva; y las personas Disciplinadas -alto en responsabilidad y promedio en los otros rasgos-, que presentaron una salud subjetiva intermedia en comparación con los otros prototipos. Finalmente, la autodisciplina y la resiliencia fueron los rasgos que discriminaron mejor la buena salud. Los hallazgos se discuten en términos de la generalización de prototipos a través de culturas.The objective of this study was to extract personality prototypes from general population of Mexico and to compare subjective health indicators between these prototypes. Participants were 994 individuals (aged 14 to 63 years). Five personality traits (Neuroticism, Extraversion, Openness, Conscientiousness, and Agreeableness) were assessed with the NEO-FFI. Subjective indicators of health (self-rated health and psychological distress) were assessed with a question regarding health status and the GHQ-12. To verify the consistency of the prototypes, the sample was divided into two age groups, young (14 to 25 years) and mature (26 to 63 years.). Three stable prototypes were recovered from both groups. Resilient individuals (low neuroticism and high in other traits) had the best subjective health; the Non-Resilient individuals (high neuroticism and low in other traits) had the worst subjective health; and Self-Disciplined individuals (high conscientiousness and medium scores in other traits) were in the middle of these extremes in subjective health. Self-discipline and resilience were most discriminative in terms of subjective health. Findings are discussed in terms of the generalization of prototypes across cultures.O objetivo deste estudo foi obter protótipos da personalidade em população geral do México e comparar a saúde subjetiva entre eles. No total, participaram 994 indivíduos de 14 a 63 anos. Avaliaram-se cinco traços de personalidade (extroversão, neuroticismo, abertura à experiência, conscienciosidade e amabilidade) com o NEO-FFI, e a saúde subjetiva com o GHQ-12, e uma pergunta sobre o estado de saúde. Para corroborar a consistência dos protótipos, dividiu-se a amostra em dois grupos de idade: jovem (de 14 a 25 anos) e adulto (de 26 a 63 anos). Como resultado, obtiveram-se três protótipos em ambos os grupos: as pessoas resilientes -baixo neuroticismo e alto nos demais traços-, os que tiveram a melhor saúde subjetiva; as não resilientes -alto neuroticismo e baixo no restante dos traços-, que apresentaram a pior saúde subjetiva, e as pessoas disciplinadas -alto em conscienciosidade e média nos outros traços-, que apresentaram uma saúde subjetiva intermediária em comparação com os outros protótipos. Finalmente, a autodisciplina e a resiliência foram os traços que melhor discriminaram a boa saúde. Os achados são discutidos em termos da generalização de protótipos através de culturas

Efficacy and safety of temozolomide in the treatment of aggressive pituitary neuroendocrine tumours in Spain

Carcinoma pituitario; RadioterapiaCarcinoma pituïtari; RadioteràpiaPituitary carcinoma; RadiotherapyCurrent guidelines recommend temozolomide as the first-line chemotherapy for aggressive pituitary neuroendocrine tumours. However, no clinical trials have been conducted to date and clinical experience is quite limited. We retrospectively analyzed 28 patients (9 women and 19 men), aged 46.6 + 16.9, with aggressive pituitary tumours (4 pituitary carcinomas and 24 aggressive adenomas) treated with temozolomide in 10 Spanish pituitary reference centres. Four patients had Cushing’s disease, 9 prolactinomas and 15 clinically non-functioning pituitary tumours (seven silent corticotroph, three silent somatotroph, one silent lactotroph, one silent gondotroph and three null-cell tumours). Median size at diagnosis was 10.5 cm3 (IQR 4.7-22.5), with cavernous sinus invasion in 88% and no metastases. Pre-temozolomide treatment, these data were 5.2 cm3 (IQR 1.9-12.3), 89.3% and 14.3% (2 intracranial and 2 spinal metastases). All patients had undergone surgery (1-5 surgeries), 25 (89.3%) had received radiotherapy (7 of them reirradiated) and 13(46.4%) had received cabergoline. One patient interrupted temozolomide prematurely. The remaining 27 patients received a median of 13 cycles (range 3-66) of 5 days every 28 days, with a mean initial dose of 265 ± 73 mg when administered alone and of 133 ± 15 mg when co-administered with radiotherapy. Eight patients (29.6%) had a significant reduction (>30%) in tumour volume and 14 (51.9%) attained tumour stabilization. After a median follow-up of 29 months (IQR 10-55), 8 out of these 22 showed disease progression. A longer progression-free survival was found in the five patients who received concomitant radiotherapy. Seven patients (25%) died (all of them because of tumour progression or complications of treatments) at 77 months (IQR 42-136) after diagnosis and 29 months (IQR 16-55) after the first dose of temozolomide. Adverse effects occurred in 18 patients (14 mild and 4 moderate or severe). In conclusion, temozolomide is an effective medical treatment for aggressive pitNET and pituitary carcinomas but is sometimes followed by tumour progression. Co-administration with radiotherapy may increase progression-free survival

Proyecto de biblioteca virtual sanitaria en un portal de cooperación para países en desarrollo

Ponencias de la Segunda Conferencia internacional sobre brecha digital e inclusión social, celebrada del 28 al 30 de octubre de 2009 en la Universidad Carlos III de MadridSe presenta el proyecto de diseño y desarrollo de una biblioteca virtual destinada a satisfacer las necesidades de información científica y de apoyo asistencial de aquellos sanitarios que se encuentran desarrollando tareas humanitarias en la cooperación internacional fuera de España. Impulsada por la Dirección General de Salud Pública de la Generalitat Valenciana, esta Biblioteca forma parte de un portal o plataforma virtual denominado AYUDSAN. La metodología seguida para realizar el proyecto de biblioteca virtual ha sido la de diseñar una página Web, en la que tras estudiar las fuentes de información más importante en materia biosanitaria, se ha realizado una amplia selección de recursos de acceso abierto y de carácter gratuito, elaborado un formulario Web y facilitado una dirección electrónica para que los usuarios puedan realizar sus consultas a través de un servicio de referencia virtual

Treatment adherence to pegvisomant (somavert®) in Spain: PEGASO study

This is a pre-copyedited, author-produced version of an article accepted for publication in Endocrine Reviews following peer review. The version of record is avaliable online at Oxford University web page

Patient-reported outcomes measures (PROMs) and patient-reported experience measures (PREMs) of COVID-19 telerehabilitation: Prospective pilot program

Telemedicine is proving to be a useful tool in the telemonitoring of respiratory patients and telerehabilitation programs. The use of telemedicine has been proposed by the main medical societies because of the limited resources and the healthcare workers infection risk in the Coronavirus Disease 2019 (COVID-19) pandemic. The aim of this pilot program is to evaluate the feasibility of COVID-19 telerehabilitation program from the hospital to the home with clinical, functional and patient satisfaction outcomes. Rehabilitation was initiated in the hospital by a physiotherapist and complemented by Estoi (a mobile application), which was continued at home with telemonitoring and messaging with the medical team. Patients' habitual use of smartphones was not queried for inclusion. Sixteen patients were consecutively enrolled, 47% women with a mean age of 63 years old. 50% of patients completed >= 15 rehabilitation sessions. In total, 88% of patients referred that the mobile application incentive them to do more physical therapy, and 63% would choose telerehabilitation instead of center-based rehabilitation for new rehabilitation programs. Patient satisfaction (0-10) for the mobile application was 8.4 and 8.9 for the telerehabilitation program. Beginning telerehabilitation in the hospital could increase the efficacy and efficiency of physical therapy, which is safe for patients and healthcare workers. Following at home, this telerehabilitation program seems to encourage and empower patients who have reported high satisfaction. Further randomized studies with larger numbers of patients and multicenter studies are required to evaluate these results

New developments in lycopene analysis by spectroscopic and chromatographic techniques, accompanied by mathematical modelling

Comunicación Oral sobre los resultados obtenidos en el estudio de las propiedades del lycopeno presente en el tomate como compuesto bioactivo. Se realizó la identificación y cuantificación por diferentes metodologías experimentales. Se muestran los resultados analíticos comapartivamente con distintas técnicas