Washington University Record, May 9, 2003

https://digitalcommons.wustl.edu/record/1968/thumbnail.jp

Graduates' perceptions about the acquired clinical competences in undergraduate study of medicine in National University of the Northeast, UNNE, Argentina

El objetivo de la investigación es evaluar la calidad y pertinencia de la educación médica impartida en la Carrera de Medicina de la Universidad Nacional del Nordeste (Argentina), a través del estudio de las percepciones de los graduados, con respecto a: la educación médica recibida, las competencias clínicas adquiridas y la adecuación de la formación a las actuales exigencias de la profesión médica. Se compara, además, el perfil real de los graduados con el perfil profesional ideal de la Carrera de Medicina, que define las competencias clínicas a desarrollar para formar un médico general, según el modelo curricular establecido. El estudio se basa en una encuesta a 439 graduados, de hasta diez años de egreso, de la ciudad de Corrientes (Argentina). Según los resultados obtenidos, los graduados evaluaron, en la educación médica recibida, como satisfactorios, principalmente, aspectos tradicionales (ej. formación teórica). Y, consecuentemente, se consideraron mejor capacitados en competencias médicas tradicionales (diagnósticas o exploratorias) y menos formados en las nuevas competencias, que hoy se exigen a los médicos (ej. trabajo en equipo). Significativamente, los graduados hicieron evaluaciones diferenciales sobre la formación, privilegiando los contextos de prácticas de mayor interacción con pacientes. A partir del estudio, podría sostenerse que en los procesos de modernización y cambio curricular de la educación médica, es fundamental, por una parte, la coherencia entre objetivos institucionales, perfil profesional propuesto y modelo curricular implementado. Pero, además, poseen una particular importancia los contextos de aprendizaje y las funciones centralizadoras de los docentes, como responsables de los procesos de socialización, a través de los cuales los jóvenes adquieren conocimientos, actitudes y valores, que condicionarán significativamente el modelo médico que efectivamente se forma.The investigation intends to evaluate the quality and pertinence relevancy of the medical education, in the Career of Medicine of the National University of the Northeast (Argentina), according to the perceptions of the graduate ones, about: the received medical education, the acquired clinical competences and the adaptation of the medical formation to the demands of the profession, in the current systems of health. The real profile of the graduate ones is compared with the ideal profile of the Career of grade Medicine, where they are defined the clinical competences to develop in a general physician. A survey was applied at 439 graduate, of up to ten years of expenditure, of the city of Corrientes (Argentina). The results show that: the graduate ones considered "satisfactory", preferably, traditional aspects of the education, for example, the theoretical teaching and the used methods. Consequently, they were considered they are better qualified in traditional medical competences, as the diagnostic ones (that continue being central in the current medical education) and the initial physical explorations and anamnesis. Of the data it is possible to infer that, in the modernization processes and curricular change, it is fundamental the coherence among the proposed professional profile - ideal - and the curricular model implemented in the career, to achieve such purposes. Also, it is evidenced in the graduate, examined, the preponderant rol of the teacher one, in the transmission of knowledge and skills and, mainly, in the formation of values and attitudes about the medical model that transmits.Fil: Humberto, Blanca Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina; ArgentinaFil: Bluvstein, Samuel. Universidad Nacional del Nordeste. Facultad de Medicina; ArgentinaFil: Melis, Ingrid G.. Universidad Nacional del Nordeste. Facultad de Medicina; ArgentinaFil: González, Marcelo A.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentin

Credibilidade das políticas econômicas no Brasil: uma análise empírica do período 1991-1998

The development of credibility and temporal inconsistency literature has contributed to non inflationary policy formulation in many countries. When a policy is implemented, the governmental credibility is pointed out as the main problem faced by policymakers. In this paper, credibility of economic policy is estimated analyzing the relationship between real interest rate differential and unemployment. The results suggest that the economic policy was not credible in sub-periods 1 and 4 (February 1991 to December 1992 and July 1996 to July 1998) and credible in sub-period 3 (June 1994 to June 1996). In sub-period 2 it was not possible to detect if the economic policy implemented was credible or not

Design and Fabrication of Flexible Copper Sensor Decorated with Bismuth Micro/Nanodentrites to Detect Lead and Cadmium in Noninvasive Samples of Sweat

The use of economic methods to design and fabricate flexible copper sensors decorated with bismuth micro/nanodentrites for the detection of lead and cadmium in sweat is demonstrated. The flexible copper sensors were constructed with simple and cost-effective materials; namely, flexible and adhesive conductive copper tape, adhesive label containing the design of a three-electrode electrochemical system, and nail polish or spray as a protective layer. The flexible copper device consisted of a working electrode decorated with bismuth micro/nanodentrites using an electrodeposition technique, a copper pseudo-reference and copper counter electrodes. Under optimal experimental conditions, the flexible sensing platform showed excellent performance toward the detection of lead and cadmium using differential pulse anodic stripping voltammetry (DPAdSV) in a wide linear range from 2.0 μM to 50 μM with acceptable reproducibility and repeatability, and limits of detection and quantification of 5.36 and 17.9 μM for Cd2+ ions and 0.76 μM and 2.5 for Pb2+ ions. Studies of addition and recovery in spiked artificial sweat sample were performed, with a recovery of 104.6%. The flexible copper device provides a great opportunity for application in wearable perspiration-based healthcare systems or portable sensors to detect toxic metals in biological samples

‘Candidatus Liberibacter asiaticus’ Multimeric LotP Mediates Citrus sinensis Defense Response Activation

‘Candidatus Liberibacter asiaticus’ is known as the most pathogenic organism associated with citrus greening disease. Since its publicized emergence in Florida in 2005, ‘Ca. L. asiaticus’ remains unculturable. Currently, a limited number of potential disease effectors have been identified through in silico analysis. Therefore, these potential effectors remain poorly characterized and do not fully explain the complexity of symptoms observed in citrus trees infected with ‘Ca. L. asiaticus.’ LotP has been identified as a potential effector and have been partially characterized. This protein retains structural homology to the substrate binding domain of the Lon protease. LotP interacts with chaperones like GroEL, Hsp40, DnaJ, and ClpX and may exercise its biological role through interactions with different proteins involved in proteostasis networks. Here, we evaluate the interactome of LotP—revealing a new protein–protein interaction target (Lon-serine protease) and its effect on citrus plant tissue integrity. We found that via protein–protein interactions, LotP can enhance Lon protease activity, increasing the degradation rate of its specific targets. Infiltration of purified LotP strained citrus plant tissue causing photoinhibition and chlorosis after several days. Proteomics analysis of LotP tissues recovering after the infiltration revealed a large abundance of plant proteins associated with the stabilization and processing of mRNA transcripts, a subset of important transcription factors; and pathways associated with innate plant defense were highly expressed. Furthermore, interactions and substrate binding module of LotP suggest potential interactions with plant proteins, most likely proteases.Fil: Merli, Marcelo Luciano. University of Florida. Department of Microbiology and Cell Science; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Padgett Pagliai, Kaylie A.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Cuaycal, Alexandra E.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: García, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Lorca, Graciela L.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Gonzalez, Claudio F.. University of Florida. Department of Microbiology and Cell Science; Estados Unido

CHEMICAL CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROETHANOLIC CRUDE EXTRACT OF EUGENIA FLORIDA DC (MYRTACEAE) LEAVES

Objective: The present study aimed to characterize and quantify the total phenolics, flavonoids and tannins in a hydroethanolic crude extract (70% (v/v) (EB)) of the leaves of E. florida DC, as well as to evaluate the antimicrobial activity of the extract against different species of micro-organisms.Methods: EB was characterized using a mass spectrometer equipped with a direct insertion device for in-stream injection (FIA). Quantitative analyses of major compounds were carried out by spectrophotometry. In addition, we evaluated the sensitivity profiles of different strains of yeast and bacteria against different concentrations of EB.Results: The classes found were in agreement with those described in the literature: flavonoids, tannins, phenolic acids and saponins. EB showed levels of phenolic compounds, flavonoids and tannins equal to 25.82 mg gallic acid equivalents per gram of extract (EAG/g), 8.42 mg quercetin equivalents per gram of extract (EQ/g) and 7.30 mg tannic acid equivalents per gram of extract (AT/g), respectively. In the analysis of antimicrobial activity, EB was more active against yeasts but was not effective against the bacteria used in the test.Conclusion: We can conclude that E. florida DC has antimicrobial potential, due to the presence of bioactive secondary metabolites.Keywords: Phenolics, Micro-organisms, Mass spectrometer, Yeasts, Bacteria, PotentialÂ

Human Hantavirus Infection, Brazilian Amazon

Tropical Medicine Foundation of Amazonas. Manaus, AM, Brasil / Amazonas State University. Manaus, AM, Brasil / Nilton Lins University Center. Manaus, AM, Brasil.Tropical Medicine Foundation of Amazonas. Manaus, AM, Brasil / Amazonas State University. Manaus, AM, Brasil / Nilton Lins University Center. Manaus, AM, Brasil / University of Brasília. Brasília, DF, Brasil.Tropical Medicine Foundation of Amazonas. Manaus, AM, Brasil.Tropical Medicine Foundation of Amazonas. Manaus, AM, Brasil / Amazonas State University. Manaus, AM, Brasil / Nilton Lins University Center. Manaus, AM, Brasil.Health Surveillance Foundation. Manaus, AM, Brasil.Ministry of Health. Brasília, DF, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Tropical Medicine Foundation of Amazonas. Manaus, AM, Brasil / Amazonas State University. Manaus, AM, Brasil / Nilton Lins University Center. Manaus, AM, Brasil / University of Brasília. Brasília, DF, Brasil