1,130 research outputs found

    Application of biophysical factors and molecular markers to explain spatial genetic structure in strawberry tree using GIS tools

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    The strawberry tree (Arbutus unedo L.) is a native species, water stress and low fertility soils tolerant, actively resistant to wildfires and widely distributed in Portugal. The fruit is used in the spirit production, the main source of income. Red fruits, with antioxidant potential, represent, also, a new market opportunity. The geographical isolation and extinction-recolonization dynamics are two factors causing strong genetic structure in metapopulations. We investigated how history, geography, and geoclimatic factors have affected population genetic structure, local adaptation, and, ultimately, its phylogeography. We examined patterns and levels of genetic diversity with nuclear microsatellites and cpDNA haplotypes in populations from across the species range. Under the project ARBUTUS (PTDC/AGR-FOR/3746/2012, Arbutus unedo plants and products quality improvement for the agro-forestry sector) 30 trees were selected, georeferenced, and leaves sampled, in 15 natural populations distributed throughout the country. With GIS tools, the stands were ecologically characterized, at a local scale, using lithology, topography, soil type, vegetation and wildfires records. This data was further used to distinguish Local Landscape Units (LLU) associated with each population. The populations were further clustered using large scale biogeographic and vegetation successions information and, this a priori hierarchy, together with the genetic structure information, was used to explain the species phytogeography. We aimed at finding the historical population demographic scenarios to explain the current patterns of genetic structure and diversity unfold for the species. The obtained information will be used in the species improvement, management and design of conservation programs

    Immature stages of Spodoptera eridania (Lepidoptera: noctuidae): developmental parameters and host plants.

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    This study aimed to detail the temporal and morphological parameters of the immature stages of southern armyworm Spodoptera eridania (Stoll, 1782) with larvae feed on artificial diet, under controlled conditions (25?±?1°C, 70?±?10% relative humidity and 14-h photophase) and gather information about their larval host plants. The viability of the egg, larval, pupal, and prepupal stages was 97.82, 93.62, 96.42, and 97.03%, respectively. The average duration of the egg, larval, pupal, and pre-pupal stages was 4.00, 16.18, 1.58, and 9.17 d, respectively. During the larval stage, 43.44% of females passed through seven instars, observing that the female's development was significant slower than males. The female larvae that developed through six and seven instars exhibited a mean growth rate of 1.52 and 1.44, respectively. Female pupae were significantly larger, exhibiting faster development than males. The rearing method proved to be adequate, providing more detailed observations of the biological cycle, especially at the larval stage, and resulting in an overall survival of almost 85%. Two hundred two plant species belonging to 58 families are listed as natural hosts for S. eridania, mainly including Asteraceae, Fabaceae, Solanaceae, Poaceae, Amaranthaceae, and Malvaceae

    Association between differential gene expression and body mass index among endometrial cancers from The Cancer Genome Atlas Project

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    The Cancer Genome Atlas (TCGA) identified four integrated clusters for endometrial cancer (EC): POLE, MSI, CNL and CNH. We evaluated differences in gene expression profiles of obese and non-obese women with EC and examined the association of body mass index (BMI) within the clusters identified in TCGA

    3-Hydroxy-2-phenyl-2,3,3a,7a-tetrahydro-1H,5H-pyrano[3,2-b]pyrrol-5-one: crystal structure and Hirshfeld surface analysis

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    The title isoaltholactone derivative, C13H13NO3, has an NH group in place of the ether-O atom in the five-membered ring of the natural product. The five-membered ring is twisted about the N—C bond linking it to the six-membered ring, which has a half-chair conformation with the O atom connected to the ether-O atom lying above the plane defined by the remaining atoms. The dihedral angle between the mean planes of the rings comprising the fused-ring system is 75.10 (8)°. In the crystal, hydroxy-O—H...N(amine) hydrogen bonding sustains linear supramolecular chains along the a axis. Chains are linked into a three-dimensional architecture via amine-N—H...π(phenyl) and phenyl-C—H...O(hydroxy) interactions. The influence of the amine-N—H...π(phenyl) contact on the molecular packing is revealed by an analysis of the Hirshfeld surface

    Twenty five years of strategies, methodologies and reactions in organic chemistry

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    An overview of the developments that occurred in the field of organic chemistry in Brazil in the last 25 years is presented. These developments are briefly compared to those observed worldwide, including some modern trends. The main source of information was the annual meeting of the Brazilian Chemical Society (RASBQ) covering the period 1979-2001 and the biennial Brazilian Meeting on Organic Synthesis (BMOS). All the contributions presented at these two meetings were classified according to six main indicatives, to permit a discussion about the past, present and future activities of Organic Chemistry in Brazil.748

    Immature development of Spodoptera dolichos (Fabricius) (Lepidoptera: noctuidae).

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    We provide detailed temporal and morphological parameters of the immature stages of Spodoptera dolichos (Fabricius) larvae fed on artificial diet under controlled conditions (25?±?1°C, 70?±?10% RH, and 14 h photophase). The viability of the egg, larval, pupal, and prepupal stages was 97.5%, 97.0%, 93.1%, and 98.9%, respectively. The average duration of the egg, larval, prepupal, and pupal stages was 5.0, 23.4, 3.2, and 21.5 days, respectively. Females took longer at the larval stage than males, with 10.5% of them having seven instars. The growth rate of female larvae that developed through six and seven instars was 1.72 and 1.54, respectively. Female pupae were significantly larger, exhibiting slower development than males

    AlteraçÔes de açĂșcares e amido em Banana 'Grande Naine' durante o amadurecimento.

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    A banana do cultivar 'Grande Naine' pertence ao grupo AAA, subgrupo Cavendish, com grande capacidade produtiva. Durante o desenvolvimento a banana acumula amido, podendo atingir acima de 20% do peso fresco do fruto. ApĂłs o pico de etileno e concomitante pico respiratĂłrio esta reserva Ă© rapidamente mobilizada, podendo cair para nĂ­veis abaixo de 1% ao mesmo tempo em que ocorre a elevação na concentração de sĂłlidos solĂșveis para valores prĂłximos a 12%. Esse trabalho teve como objetivo avaliar as transformaçÔes relativas aos teores de amido e açĂșcares ao longo do amadurecimento de banana do cultivar 'Grande Naine', gerando informaçÔes sobre esta cultivar para o programa de melhoramento genĂ©tico. Foram realizadas anĂĄlises de teor de amido, teor de sĂłlidos solĂșveis, açĂșcares totais e redutores. O delineamento experimental foi IC com 6 repetiçÔes de 2 frutos. O avanço pelos estĂĄdios de amadurecimento refletiu na redução dos teores de amido e aumento dos sĂłlidos solĂșveis. As principais mudanças nas variĂĄveis estudadas foram observadas entre os estĂĄdios 3 e 4. Com os dados obtidos foi possĂ­vel acompanhar o comportamento do metabolismo dos açĂșcares da banana 'Grande Naine' produzida em Cruz das Almas
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