Estimation of badger abundance using faecal DNA typing

1.Wildlife management and conservation programmes often require accurate information on population density, but this can be difficult to obtain, particularly when the species in question is nocturnal or cryptic. Badger populations in Britain are of intense management interest because they are a wildlife reservoir host of bovine tuberculosis (TB). Attempts to manage this infection in badgers, whether by population control or vaccination, require reliable methods of estimating population size. In addition, such estimates are also required to support research into badger ecology and TB epidemiology. Currently, the most accurate estimates of local badger population size are obtained from labour-intensive and time-consuming mark–recapture studies. 2. In recent years, DNA has been successfully extracted from the faeces of certain mammals, and used to generate a genetic profile of the defecating individual. Here we report on an application of this technology to estimate badger abundance.3.Faecal samples were collected on 10 consecutive days from every freshly deposited dropping at latrine sites close to occupied setts in three badger social groups. Badger DNA was extracted from 89% of samples, and 20 different individuals were reliably identified. The genotypes derived from the faecal samples were compared with those obtained from blood or samples from badgers live trapped at the same setts.4.The faecal genotypes from badgers with known trap histories revealed that latrines were used equally by males and females, and by badgers ranging in age from cubs(< 1 year old) to 9 years old. Individual badgers used the latrines on between one and six different nights. Rarefaction analysis produced abundance estimates that closely matched those obtained from live trapping. 5.Synthesis and applications. Systematic sampling and genetic typing of fresh faeces from badger latrines can provide data that can be used to estimate abundance accurately.This approach requires considerably less human resources than repeated live trapping and mark–recapture. The technique may be valuable for future badger research and management in relation to bovine TB, where accurate estimates of abundance at a local scale are required

History repeats? : the rise of the new middle classes in the developing world

1. Although the ability to detect chemical cues is widespread in many organisms, it is surprising how little is known about the role of chemical communication in avian life histories. Nowadays, growing evidence suggests that birds can use olfaction in several contexts. However, we still do not know the role of bird olfaction in one of the most important determinants of survival, predator detection. 2. Blue tits, Cyanistes caeruleus L., were exposed to chemical cues of: (i) mustelid (predator), (ii) quail (odorous control); or (iii) water (odourless control) inside the nest-box where they were provisioning 8-day-old nestlings. 3. We show that blue tits were able to detect the chemical cues and showed antipredatory behaviours to cope with the risk of predation. Birds delayed their entry to the nest-box, and they perched on the hole of the nest-box and refused to enter more times when they found predator scent than control scents inside the nest-box. In addition, birds decreased the time spent inside the predator-scented nest-box when feeding nestlings. 4. The discovery of the ability of birds to use chemical cues of predators to accurately assess predation may help to understand many aspects of bird life histories that have been neglected until now.Peer reviewe

The local adsorption structure of methylthiolate and butylthiolate on Au(1 1 1): a photoemission core-level shift investigation

Measurements of the core-level shifts in Au 4f photoemission spectra from Au(1 1 1) at different coverages of methylthiolate and butylthiolate are reported. Adsorption leads to two components in addition to that from the bulk, one at lower photoelectron binding energy attributed to surface atoms not bonded to thiolate species, while the second component has a higher binding energy and is attributed to Au atoms bonded to the surface thiolate. The relative intensities of these surface components for the saturation coverage (mainly (√3 × √3)R30°) phases are discussed in terms of different local adsorption sites in a well-ordered surface, and favour adsorption of the thiolate species atop Au adatoms. Alternative interpretations that might be consistent with an Au-adatom-dithiolate model are discussed, particularly in the context of the possible influence of reduced coverage associated with a disordered surface. Marked differences from previously-reported results for longer-chain alkylthiolate layers are highlighted

Factors Associated with Revision Surgery after Internal Fixation of Hip Fractures

Background: Femoral neck fractures are associated with high rates of revision surgery after management with internal fixation. Using data from the Fixation using Alternative Implants for the Treatment of Hip fractures (FAITH) trial evaluating methods of internal fixation in patients with femoral neck fractures, we investigated associations between baseline and surgical factors and the need for revision surgery to promote healing, relieve pain, treat infection or improve function over 24 months postsurgery. Additionally, we investigated factors associated with (1) hardware removal and (2) implant exchange from cancellous screws (CS) or sliding hip screw (SHS) to total hip arthroplasty, hemiarthroplasty, or another internal fixation device. Methods: We identified 15 potential factors a priori that may be associated with revision surgery, 7 with hardware removal, and 14 with implant exchange. We used multivariable Cox proportional hazards analyses in our investigation. Results: Factors associated with increased risk of revision surgery included: female sex, [hazard ratio (HR) 1.79, 95% confidence interval (CI) 1.25-2.50; P = 0.001], higher body mass index (fo

Aposematism and crypsis combined as a result of distance dependence: functional versatility of the colour pattern in the swallowtail butterfly larva

The idea that an aposematic prey combines crypsis at a distance with conspicuousness close up was tested in an experiment using human subjects. We estimated detectability of the aposematic larva of the swallowtail butterfly, Papilio machaon, in two habitats, by presenting, on a touch screen, photographs taken at four different distances and measuring the time elapsed to discovery. The detectability of larvae in these images was compared with images that were manipulated, using existing colours either to increase or decrease conspicuousness. Detection time increased with distance for all colourations. However, at the closest distance, detection time was longer for the larvae manipulated to be more cryptic than for the natural and more conspicuous forms. This indicates that the natural colouration is not maximally cryptic at a short distance. Further, smaller increments in distance were needed to increase detection time for the natural than for the conspicuous larva. This indicates that the natural colouration is not maximally conspicuous at longer distances. Taken together, we present the first empirical support for the idea that some colour patterns may combine warning colouration at a close range with crypsis at a longer range. The implications of this result for the evolution of aposematism are discussed