Magnetic moments of 68^{68}Cug,m^{g,m} and 70^{70}Cug,m1,m2^{g,m_{1},m_{2}} nuclei measured by in-source laser spectroscopy

We have obtained information on the atomic hyperfine splitting and, hence, on magnetic moments in neutron rich $^{68, 70}$Cu isotopes by scanning the frequency of the narrow-band laser of the first excitation step in the resonance ionization laser ion source. The deduced magnetic moments are $\mu( ^{68}$Cu$^{g}$, I$^{\pi}$ = 1$^+$) = +2.48(2)(7)$\mu_{N}$ ; $\mu(^{68}$Cu$^{m}$, I$^{\pi}$=6$^{-}$) = +1.24(4)(6)$\mu_{N}$ and $\mu(^{70}$Cu$^{m_{2}}$, I$^{\pi}$=1$^{+}$) = +1.86(4)(6)$\mu_{N}$ ; $\mu(^{70}$Cu$^{g}$, I$^{\pi}$=6$^{-}$) = +1.50(7)(8)$\mu_{N}$. The results of the scans analysis point out on existence of a new isomer in $^{70}$Cu$^{m_{1}}$. It's deduced magnetic moment is (-)3.50(7)(11)$\mu_{N}$ that is in a good agreement with I$^{\pi}$=3$^{-}$ assignment. The method of in-source atomic spectroscopy, as well as the analysis of the obtained data, is described. The results are discussed in terms of single-particle configurations coupled to the $^{68}$Ni core

On-line yields obtained with the ISOLDE RILIS

The ISOLDE resonance ionization laser ion source (RILIS) allows to ionize efficiently and selectively many metallic elements. In recent yield surveys and on-line experiments with the ISOLDE RILIS we observed $^{23-34}$Mg , $^{26-34}\!$Al , $^{98-132}$Cd , $^{149}$Tb , $^{155-177}\!$Yb , $^{179-200}$Tl , $^{183-215}$Pb and $^{188-218}$Bi. The obtained yields are presented together with measured release parameters which allow to extrapolate the release efficiency towards more exotic (short-lived) nuclides of the same elements

KLC1-ALK: A Novel Fusion in Lung Cancer Identified Using a Formalin-Fixed Paraffin-Embedded Tissue Only

The promising results of anaplastic lymphoma kinase (ALK) inhibitors have changed the significance of ALK fusions in several types of cancer. These fusions are no longer mere research targets or diagnostic markers, but they are now directly linked to the therapeutic benefit of patients. However, most available tumor tissues in clinical settings are formalin-fixed and paraffin-embedded (FFPE), and this significantly limits detailed genetic studies in many clinical cases. Although recent technical improvements have allowed the analysis of some known mutations in FFPE tissues, identifying unknown fusion genes by using only FFPE tissues remains difficult. We developed a 5′-rapid amplification of cDNA ends-based system optimized for FFPE tissues and evaluated this system on a lung cancer tissue with ALK rearrangement and without the 2 known ALK fusions EML4-ALK and KIF5B-ALK. With this system, we successfully identified a novel ALK fusion, KLC1-ALK. The result was confirmed by reverse transcription-polymerase chain reaction and fluorescence in situ hybridization. Then, we synthesized the putative full-length cDNA of KLC1-ALK and demonstrated the transforming potential of the fusion kinase with assays using mouse 3T3 cells. To the best of our knowledge, KLC1-ALK is the first novel oncogenic fusion identified using only FFPE tissues. This finding will broaden the potential value of archival FFPE tissues and provide further biological and clinical insights into ALK-positive lung cancer

Residential exposure to motor vehicle emissions and the risk of wheezing among 7-8 year-old schoolchildren: a city-wide cross-sectional study in Nicosia, Cyprus

<p>Abstract</p> <p>Background</p> <p>Several studies have reported associations between respiratory outcomes in children and a range of self-reported, administrative or geographical indicators of traffic pollution. First-time investigation into the frequency of asthmatic symptoms among 7-8 year-old Cypriot children in 1999-2000 showed increased prevalence in the capital Nicosia compared to other areas. Geographical differences on an island the size of Cyprus may reflect environmental and/or lifestyle factors. This study investigates the relationship between self-reported symptoms and residential exposure to motor vehicle emissions among Nicosia schoolchildren.</p> <p>Methods</p> <p>The addresses of children in the metropolitan area of Nicosia who participated in the original survey (N = 1,735) were geo-coded and the level of exposure of each child was assessed using distance- and emission-based indicators (i.e. estimated levels of particulate matter and nitrogen oxides emissions due to motor vehicles on main roads around the residence). Odds ratios of wheezing and asthma diagnosis in relation to levels of exposure were estimated in logistic regression models adjusting for person-based factors, co-morbidity and intra-school clustering.</p> <p>Results</p> <p>We found an increased risk of wheezing at distances less than 50 m from a main road and/or only among those experiencing the highest levels of exposure. The strongest effect estimates were observed when exposure was defined in terms of the cumulative burden at all roads around the residence. Adjusted odds ratios for current wheezing were 2.33 (95% CI 1.27, 4.30) amongst the quartile of participants exposed to the highest levels of PM at all roads 50 m of their residence and 2.14 (95% CI 1.05, 4.35) for NOx, with no effect at intermediate levels of exposure. While the direction of effect was apparent at longer distances, differences were generally not statistically significant.</p> <p>Conclusions</p> <p>Children experiencing the highest burden of emissions in Nicosia seem to be at a higher risk of reporting asthmatic symptoms. Due to the small number of children residing at close proximity to main roads and lack of evidence of risk at intermediate levels of exposure or longer distances, the observed pattern alone does not explain the generally higher prevalence observed in urban Nicosia compared to other areas.</p