Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens

For decades, low doses of antibiotics have been used widely in animal production to promote growth. However, there is a trend to reduce this use of antibiotics in feedstuffs, and legislation is now in place in Europe to prohibit their use in this way. As a consequence, economically important diseases, such as necrotic enteritis (NE) of chickens, that are caused by Clostridium perfringens have become more prevalent. Recent research is creating a paradigm shift in our understanding of the pathogenesis of NE and is now providing information that will be necessary to monitor and control the incidence of NE in poultry

Functional Analysis of the VirSR Phosphorelay from Clostridium perfringens

Toxin production in Clostridium perfringens is controlled by the VirSR two-component signal transduction system, which comprises the VirS sensor histidine kinase and the VirR response regulator. Other studies have concentrated on the elucidation of the genes controlled by this network; there is little information regarding the phosphorelay cascade that is the hallmark of such regulatory systems. In this study, we have examined each step in this cascade, beginning with autophosphorylation of VirS, followed by phosphotransfer from VirS to VirR. We also have studied the effects of gene dosage and phosphorylation in vivo. We have used random and site-directed mutagenesis to identify residues in VirS that are important for its function and have identified a region in the putative sensory domain of VirS that appeared to be essential for function. In vitro phosphorylation studies showed that VirSc, a truncated VirS protein that lacked the N-terminal sensory domain, was capable of autophosphorylation and could subsequently act as a phosphodonor for its cognate response regulator, VirR. Conserved residues of both VirS and VirR, including the D57 residue of VirR, were shown to be essential for this process. By use of Targetron technology, we were able to introduce a single copy of virR or virRD57N onto the chromosome of a virR mutant of C. perfringens. The results showed that in vivo, when virR was present in single copy, the production of wild-type levels of perfringolysin O was dependent on the presence of virS and an unaltered D57 residue in VirR. These results provide good evidence that phosphorylation is critical for VirR function

Late Quaternary slip rate on the Kern Canyon fault at Soda Spring, Tulare County, California

The Kern Canyon fault represents a major tectonic and physiographic boundary in the southern Sierra Nevada of east-central California. Previous investigations of the Kern Canyon fault underscore its importance as a Late Cretaceous and Neogene shear zone in the tectonic development of the southern Sierra Nevada. Study of the late Quaternary history of activity, however, has been confounded by the remote nature of the Kern Canyon fault and deep along-strike exhumation within the northern Kern River drainage, driven by focused fluvial and glacial erosion. Recent acquisition of airborne lidar (light detection and ranging) topography along the ∼140 km length of the Kern Canyon fault provides a comprehensive view of the active surface trace. High-resolution, lidar-derived digital elevation models (DEMs) for the northern Kern Canyon fault enable identification of previously unrecognized offsets of late Quaternary moraines near Soda Spring (36.345°N, 118.408°W). Predominately north-striking fault scarps developed on the Soda Spring moraines display west-side-up displacement and lack a significant sense of strike-slip separation, consistent with detailed mapping and trenching along the entire Kern Canyon fault. Scarp-normal topographic profiling derived from the lidar DEMs suggests normal displacement of at least 2.8 +0.6/–0.5 m of the Tioga terminal moraine crest. Cosmogenic 10Be exposure dating of Tioga moraine boulders yields a tight age cluster centered around 18.1 ± 0.5 ka (n = 6), indicating a minimum normal-sense fault slip rate of ∼0.1–0.2 mm/yr over this period. Taken together, these results provide the first clear documentation of late Quaternary activity on the Kern Canyon fault and highlight its role in accommodating internal deformation of the southern Sierra Nevada

Detection and diversity of a putative novel heterogeneous polymorphic proline-glycine repeat (Pgr) protein in the footrot pathogen Dichelobacter nodosus

Dichelobacter nodosus, a Gram-negative anaerobic bacterium, is the essential causative agent of footrot in sheep. Currently, depending on the clinical presentation in the field, footrot is described as benign or virulent; D. nodosus strains have also been classified as benign or virulent, but this designation is not always consistent with clinical disease. The aim of this study was to determine the diversity of the pgr gene, which encodes a putative proline-glycine repeat protein (Pgr). The pgr gene was present in all 100 isolates of D. nodosus that were examined and, based on sequence analysis had two variants, pgrA and pgrB. In pgrA, there were two coding tandem repeat regions, R1 and R2: different strains had variable numbers of repeats within these regions. The R1 and R2 were absent from pgrB. Both variants were present in strains from Australia, Sweden and the UK, however, only pgrB was detected in isolates from Western Australia. The pgrA gene was detected in D. nodosus from tissue samples from two flocks in the UK with virulent footrot and only pgrB from a flock with no virulent or benign footrot for >10 years. Bioinformatic analysis of the putative PgrA protein indicated that it contained a collagen-like cell surface anchor motif. These results suggest that the pgr gene may be a useful molecular marker for epidemiological studies

Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin

A novel toxin, NetB, has recently been identified in virulent avian Clostridium perfringens isolates and shown to be an essential virulence factor in a clinical necrotic enteritis isolate. To assess whether NetB is more generally associated with avian necrotic enteritis isolates we have screened a range of C. perfringens strains from geographically diverse locations for both the presence and expression of the netB gene. Forty-four isolates were derived from necrotic enteritis disease cases from Australia, Belgium, Denmark and Canada and 55 isolates from healthy chickens from Australia and Belgium. The majority of strains isolated from necrotic enteritis-affected birds were netB positive (70%) and there was an absolute correlation between the presence of netB and in vitro expression of the NetB protein. Only two of the C. perfringens isolates from healthy chickens carried netB. Sequencing of the netB gene from 23 positive isolates showed that NetB is highly conserved, with only one predicted amino acid (A168T) difference, in six isolates, compared to the published sequence. This change did not alter the in vitro activity of the NetB toxin. The gene encoding the recently discovered TpeL toxin was also screened using PCR and only found in a small proportion of NetB-positive isolates from diseased birds. A selection of NetB-negative isolates, originating from diseased birds, was unable to cause disease in a necrotic enteritis induction model. This study provides further evidence that NetB is important in pathogenesis and advances our current understanding of C. perfringens virulence factors in avian necrotic enteritis

BBN and the Primordial Abundances

The relic abundances of the light elements synthesized during the first few minutes of the evolution of the Universe provide unique probes of cosmology and the building blocks for stellar and galactic chemical evolution, while also enabling constraints on the baryon (nucleon) density and on models of particle physics beyond the standard model. Recent WMAP analyses of the CBR temperature fluctuation spectrum, combined with other, relevant, observational data, has yielded very tight constraints on the baryon density, permitting a detailed, quantitative confrontation of the predictions of Big Bang Nucleosynthesis with the post-BBN abundances inferred from observational data. The current status of this comparison is presented, with an emphasis on the challenges to astronomy, astrophysics, particle physics, and cosmology it identifies.Comment: To appear in the Proceedings of the ESO/Arcetri Workshop on "Chemical Abundances and Mixing in Stars in the Milky Way and its Satellites", eds., L. Pasquini and S. Randich (Springer-Verlag Series, "ESO Astrophysics Symposia"

IRFM T_eff calibrations for cluster and field giants in the Vilnius, Geneva, RI(C) and DDO photometric systems

Based on a large sample of disk and halo giant stars, for which accurate effective temperatures derived through the InfraRed Flux Method (IRFM) exist, a calibration of the temperature scale in the Vilnius, Geneva, RI(C) and DDO photometric systems is performed. We provide calibration formulae for the metallicity dependent T_eff vs color relations as well as grids of intrinsic colors and compare them with other calibrations. Photometry, atmospheric parameters and reddening corrections for the stars of the sample have been updated with respect to the original sources in order to reduce the dispersion of the fits. Application of our results to Arcturus leads to an effective temperature in excellent agreement with the value derived from its angular diameter and integrated flux. The effects of gravity on these T_eff vs color relations are also explored by taking into account our previous results for dwarf stars.Comment: Accepted for publication in A&

The optical companion to the binary millisecond pulsar J1824-2452H in the globular cluster M28

We report on the optical identification of the companion star to the eclipsing millisecond pulsar PSR J1824-2452H in the galactic globular cluster M28 (NGC 6626). This star is at only 0.2" from the nominal position of the pulsar and it shows optical variability (~ 0.25 mag) that nicely correlates with the pulsar orbital period. It is located on the blue side of the cluster main sequence, ~1.5 mag fainter than the turn-off point. The observed light curve shows two distinct and asymmetric minima, suggesting that the companion star is suffering tidal distortion from the pulsar. This discovery increases the number of non-degenerate MSP companions optically identified so far in globular clusters (4 out of 7), suggesting that these systems could be a common outcome of the pulsar recycling process, at least in dense environments where they can be originated by exchange interactions.Comment: accepted for publication on ApJ, 17 pages, 5 figure