1,058 research outputs found
Microbial sources of exocellular DNA in the ocean
© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Linney, M. D., Eppley, J. M., Romano, A. E., Luo, E., DeLong, E. F., & Karl, D. M. Microbial sources of exocellular DNA in the ocean. Applied and Environmental Microbiology, 88(7), (2022): e02093-21, https://doi.org/10.1128/aem.02093-21.Exocellular DNA is operationally defined as the fraction of the total DNA pool that passes through a membrane filter (0.1 μm). It is composed of DNA-containing vesicles, viruses, and free DNA and is ubiquitous in all aquatic systems, although the sources, sinks, and ecological consequences are largely unknown. Using a method that provides separation of these three fractions, we compared open ocean depth profiles of DNA associated with each fraction. Pelagibacter-like DNA dominated the vesicle fractions for all samples examined over a depth range of 75 to 500 m. Viral DNA consisted predominantly of myovirus-like and podovirus-like DNA and contained the highest proportion of unannotated sequences. Euphotic zone free DNA (75 to 125 m) contained primarily bacterial and viral sequences, with bacteria dominating samples from the mesopelagic zone (500 to 1,000 m). A high proportion of mesopelagic zone free DNA sequences appeared to originate from surface waters, including a large amount of DNA contributed by high-light Prochlorococcus ecotypes. Throughout the water column, but especially in the mesopelagic zone, the composition of free DNA sequences was not always reflective of cooccurring microbial communities that inhabit the same sampling depth. These results reveal the composition of free DNA in different regions of the water column (euphotic and mesopelagic zones), with implications for dissolved organic matter cycling and export (by way of sinking particles and/or migratory zooplankton) as a delivery mechanism.This work was supported by the Simons Collaboration on Ocean Processes and Ecology (awards 329108 to D.M.K. and E.F.D., 721252 to D.M.K., and 721223 to E.F.D.)
NuSTAR Detection Of A Cyclotron Line In The Supergiant Fast X-ray Transient IGR J17544-2619
We present NuSTAR spectral and timing studies of the Supergiant Fast X-ray
Transient (SFXT) IGR J17544-2619. The spectrum is well-described by a ~1 keV
blackbody and a hard continuum component, as expected from an accreting X-ray
pulsar. We detect a cyclotron line at 17 keV, confirming that the compact
object in IGR J17544-2619 is indeed a neutron star. This is the first
measurement of the magnetic field in a SFXT. The inferred magnetic field
strength, B = (1.45 +/- 0.03) * 10^12 G * (1+z) is typical of neutron stars in
X-ray binaries, and rules out a magnetar nature for the compact object. We do
not find any significant pulsations in the source on time scales of 1-2000 s.Comment: MNRAS Accepted. 8 pages, 4 figures, 3 table
The Swift X-ray flaring afterglow of GRB 050607
The unique capability of the Swift satellite to perform a prompt and
autonomous slew to a newly detected Gamma-Ray Burst (GRB) has yielded the
discovery of interesting new properties of GRB X-ray afterglows, such as the
steep early lightcurve decay and the frequent presence of flares detected up to
a few hours after the GRB trigger. We present observations of GRB 050607, the
fourth case of a GRB discovered by Swift with flares superimposed on the
overall fading X-ray afterglow. The flares of GRB 050607 were not symmetric as
in previously reported cases, showing a very steep rise and a shallower decay,
similar to the Fast Rise, Exponential Decay that are frequently observed in the
gamma-ray prompt emission. The brighter flare had a flux increase by a factor
of approximately 25,peaking for 30 seconds at a count rate of approximately 30
counts s-1, and it presented hints of addition short time scale activity during
the decay phase. There is evidence of spectral evolution during the flares. In
particular, at the onset of the flares the observed emission was harder, with a
gradual softening as each flare decayed. The very short time scale and the
spectral variability during the flaring activity are indicators of possible
extended periods of energy emission by the GRB central engine. The flares were
followed by a phase of shallow decay, during which the forward shock was being
refreshed by a long-lived central engine or by shells of lower Lorentz factors,
and by a steepening after approximately 12 ks to a decay slope considered
typical of X-ray afterglows.Comment: 23 pages, 5 figures, Accepted by the Astrophysical Journa
Swift Observations of GRB 050603: An afterglow with a steep late time decay slope
We report the results of Swift observations of the Gamma Ray Burst GRB
050603. With a V magnitude V=18.2 about 10 hours after the burst the optical
afterglow was the brightest so far detected by Swift and one of the brightest
optical afterglows ever seen. The Burst Alert Telescope (BAT) light curves show
three fast-rise-exponential-decay spikes with =12s and a fluence of
7.6 ergs cm in the 15-150 keV band. With an ergs it was also one of the most energetic
bursts of all times. The Swift spacecraft began observing of the afterglow with
the narrow-field instruments about 10 hours after the detection of the burst.
The burst was bright enough to be detected by the Swift UV/Optical telescope
(UVOT) for almost 3 days and by the X-ray Telescope (XRT) for a week after the
burst. The X-ray light curve shows a rapidly fading afterglow with a decay
index =1.76. The X-ray energy spectral index was
=0.71\plm0.10 with the column density in agreement with the
Galactic value. The spectral analysis does not show an obvious change in the
X-ray spectral slope over time. The optical UVOT light curve decays with a
slope of =1.8\plm0.2.
The steepness and the similarity of the optical and X-ray decay rates suggest
that the afterglow was observed after the jet break. We estimate a jet opening
angle of about 1-2Comment: 14 pages, accepted for publication in Ap
Interaction Between Functional Genetic Variation of DRD2 and Cannabis Use on Risk of Psychosis
Both cannabis use and the dopamine receptor (DRD2) gene have been associated with schizophrenia, psychosis-like experiences, and cognition. However, there are no published data investigating whether genetically determined variation in DRD2 dopaminergic signaling might play a role in individual susceptibility to cannabis-associated psychosis. We genotyped (1) a case-control study of 272 patients with their first episode of psychosis and 234 controls, and also from (2) a sample of 252 healthy subjects, for functional variation in DRD2, rs1076560. Data on history of cannabis use were collected on all the studied subjects by administering the Cannabis Experience Questionnaire. In the healthy subjects' sample, we also collected data on schizotypy and cognitive performance using the Schizotypal Personality Questionnaire and the N-back working memory task. In the case-control study, we found a significant interaction between the rs1076560 DRD2 genotype and cannabis use in influencing the likelihood of a psychotic disorder. Among cannabis users, carriers of the DRD2, rs1076560, T allele showed a 3-fold increased probability to suffer a psychotic disorder compared with GG carriers (OR = 3.07; 95% confidence interval [CI]: 1.22-7.63). Among daily users, T carrying subjects showed a 5-fold increase in the odds of psychosis compared to GG carriers (OR = 4.82; 95% CI: 1.39-16.71). Among the healthy subjects, T carrying cannabis users had increased schizotypy compared with T carrying cannabis-naïve subjects, GG cannabis users, and GG cannabis-naïve subjects (all P ≤ .025). T carrying cannabis users had reduced working memory accuracy compared with the other groups (all P ≤ .008). Thus, variation of the DRD2, rs1076560, genotype may modulate the psychosis-inducing effect of cannabis us
The Global Dominance of European Competition Law Over American Antitrust Law
The world’s biggest consumer markets – the European Union and the United States – have adopted different approaches to regulating competition. This has not only put the EU and US at odds in high-profile investigations of anticompetitive conduct, but also made them race to spread their regulatory models. Using a novel dataset of competition statutes, we investigate this race to influence the world’s regulatory landscape and find that the EU’s competition laws have been more widely emulated than the US’s competition laws. We then argue that both “push” and “pull” factors explain the appeal of the EU’s competition regime: the EU actively promotes its model through preferential trade agreements and has an administrative template that is easy to emulate. As EU and US regulators offer competing regulatory models in domains as diverse as privacy, finance, and environmental protection, our study sheds light on how global regulatory races are fought and won
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