La investigación mediante experimentos. El reto de estudiar causas y efectos

Este capítulo es una explicación sencilla de la aplicación de los experimentos a la investigación social. El texto explica en qué consiste un experimento y las ventajas que tiene investigar mediante experimentos en las ciencias sociales. Se explica un diseño experimental básico y conceptos relevantes en el diseño de los experimentos como "grupo de control", "aleatorización", etc. El texto explicita las ventajas e inconvenientes que la literarutura suele atribuir a la investigación mediante experimentos. Se completa el texto con ejemplos clásicos de uso de experimentos en investigaciones sociales: se hace referencia a varias de las investigaciones del equipo de Hovland sobre persuasión en la Universidad de Yale y al conocido experimento de Milgram sobre la obedicencia a la autoridad

La educación emprendedora en Andalucía. Un enfoque integrado

When evaluating the relevance of actions within the area of entrepreneurship education, the quite remarkable national and regional differences regarding entrepreneurial culture, entrepreneurial potential and, of course, the business structure, have to be taken into account. Based on these differences, there is a need to specifically design entrepreneurial education policies which take into account the strengths and weaknesses of the business structure of each region. This paper aims to identify the structural characteristics of the Andalusian entrepreneurial model in comparison with that of other Spanish regions. To do this, a database of over 1000 companies have been used coming from the Research Project (Ref. P09- SEJ -4857) carried out by the "SMEs and economic development" research group (SEJ -128) of the University of Seville. This information will also be complemented with regional reports from the GEM project. From these two data sources, an eminently qualitative profile of the structural weaknesses of existing businesses and their entrepreneurs in Andalusia will be established. From this analysis of regional needs, we will raise our differential proposition for an embedded education towards entrepreneurship. It will identify and include the content and pedagogical techniques necessary to overcome these weaknesses of the regional business community.Al evaluar la pertinencia de las acciones en el ámbito de la educación empresarial, las notables diferencias nacionales y regionales con respecto a la cultura emprendedora, el potencial empresarial y, por supuesto, la estructura empresarial, han de ser tenidos en cuenta. Sobre la base de estas diferencias, hay una necesidad de diseñar políticas específicas de educación emprendedora que tengan en cuenta las fortalezas y debilidades de la estructura empresarial de cada región. Este trabajo tiene como objetivo identificar las características estructurales del modelo empresarial andaluz en comparación con el de otras regiones españolas. Para ello, se ha usado una base de datos de más de 1.000 empresas procedente del Proyecto de Investigación (Ref. P09- SEJ -4857) llevado a cabo por el grupo de investigación "PYME y desarrollo económico” (SEJ -128) de la Universidad de Sevilla. Esta información también se complementará con los informes regionales del proyecto GEM. A partir de estas dos fuentes de datos, se establecerá un perfil eminentemente cualitativo de las debilidades estructurales de las empresas existentes en Andalucía y de sus empresarios. A partir de este análisis de las necesidades regionales, vamos a plantear nuestra propuesta diferencial para una educación emprendedora integrada. Se identificará e incluirá el contenido y las técnicas pedagógicas necesarias para superar estas debilidades de la comunidad empresarial regional

Preparation, separation and characterization of gold nanoparticle encapsulated liposomes and their potential application as analytical reagents

III Encuentro sobre Nanociencia y Nanotecnología de Investigadores y Tecnólogos Andaluce

Estudio de la biocompatibilidad in vitro de polimeros metacrílicos derivados de pirrolidona-ina

Este estudio analiza la biocompatibilidad de nuevos biomateriales a base de hidrogeles metacrílicos como el poli-[metacrilato de 2-etil-(2-pirrolidona)] (PEPM) y la poli-[metacrilato de 2-etil-(2-pirrolidina)], en comparación con referentes comerciales tales como la poli-(vinil pirrolidona) (PVP) y la poli-(N-N´-dimetilacrilamida) (PDMA). Se utilizaron propiedades bioquímicas, mediante los ensayos de MTT y Alamar Blue, para conocer el efecto de citotoxicidad y proliferación en cultivo de fibroblastos (células VERO), sobre los biomateriales metacrílicos. Adicionalmente, por microscopía óptica y electrónica fueron analizados el grado de adhesión, morfología, muerte celular, proliferación, contracción celular, etc. Además se estudiaron las relaciones entre citotoxicidad y estructura química. En el MTT y en el análisis óptico se obtuvo baja citotoxicidad para PEPM y PVP, media en PEPyM y alta en PDMA. En el Alamar Blue y SEM se produjo una media proliferación-adhesión en PEPM y PEPyM, alta en PVP y muerte celular en PDMA. Sus apropiadas características físico-químicas y su aceptable grado de biocompatibilidad les hacen buenos candidatos en aplicaciones tales como lentes de contacto, sistemas de liberación de fármacos e ingeniería de tejidos.This study analyzed biocompatibility of new biomaterials based on the methacrylic hydrogels derived poly-[2-ethyl-(2-pyrrolidone) methacrylate] (PEPM) and poly-[2-ethyl-(2-pyrrolidine) methacrylate] (PEPyM), in comparison with references such as commercial poly-(vinyl pyrrolidone) (PVP) and poly-(N, N-dimethylacrylamide) (PDMA). The biochemical properties were used to know citotoxicity and proliferation effect over fibroblast culture (VERO cells) in contact with the methacrylic biomaterials, using MTT and Alamar Blue assays. Additionally, were analyzed adhesion grade, morphology, death cell presence, proliferation, cell contraction, etc, by optical and scanning electronic microscopy (SEM). The relationships between their chemical structures and cytotoxicity were studied. In MTT and optical analysis, low cytotoxicity was found for PEPM and PVP, medium in the case of PEPyM, and high for PDMA. In Alamar Blue and SEM, were produced a medium proliferation and adhesion rate for PEPM and PEPyM, high in PVP, and cellular death for PDMA. Their appropriates physico-chemical characteristics and approval biocompatibility grade make them good candidates in applications such as contact lenses, drug release systems and tissue engineering

Results for Greenland halibut, American plaice and Atlantic cod of the Spanish survey in NAFO Div. 3NO for the period 1997-2015

Greenland halibut (Reinhardtius hippoglossoides), American plaice (Hippoglossoides platessoides) and Atlantic cod (Gadus morhua) indices from the bottom trawl survey that Spain carries out in Spring since 1995 in Div. 3NO of the NAFO Regulatory Area are presented. Biomass, stratified mean catches and mean number per tow for the three species are presented since 1997, year in which the survey extended the depth strata. Mean catch per town, length distribution and age distribution are presented for the last five years (2011-2015). Greenland halibut biomass and abundance estimates presented a decreasing trend since 1999, cut in year 2007 with an increase, reaching in 2009 the highest value in the series. In 2011 the biomass drops under the 2008 value, being stable since then with a slight increase in 2015. In last years it can be seen a presence of juveniles, mainly in 2004, but the greatest lengths have failed, although in 2009 there is a quite good presence of individuals of ages 6-7 and in 2010 between 5-7. In 2011-2015 the presence of all ages is poor, although in 2015 an increase in the range of the length can be seen with regards to last years. For American plaice we can see an increasing trend along the whole period, reaching a maximum of mean catch and number in 2006. The greatest recruitment in the presented series occurred in 2004 and we can follow their mode along the years. No good recruitments were seen since then, but a discrete occurrence of individuals of 12-20 cm appears in 2015. For Atlantic cod it can be seen a general decreasing in the biomass between 2002 and 2005 and an increasing since then, especially in 2006 and, higher, in 2009-2011, decreasing again in 2012-2013 but reaching the maximum in the series in 2014. In 2015 the biomass is in the level of the 2012 biomass but the mean number is almost the same as in 2014. In 2007-2008 the youngest length classes were much over the rest of the length classes. With the 2006 cohort the series reaches the maximum number of its historical values at five years in 2011. There have been no good recruitments since 2009, although in 2015 a discrete presence of individuals of age 1 can be seen. MaterialPostprint0,000

Controlled Anisotropic Deformation of Ag Nanoparticles by Si Ion Irradiation

The shape and alignment of silver nanoparticles embedded in a glass matrix is controlled using silicon ion irradiation. Symmetric silver nanoparticles are transformed into anisotropic particles whose larger axis is along the ion beam. Upon irradiation, the surface plasmon resonance of symmetric particles splits into two resonances whose separation depends on the fluence of the ion irradiation. Simulations of the optical absorbance show that the anisotropy is caused by the deformation and alignment of the nanoparticles, and that both properties are controlled with the irradiation fluence.Comment: Submitted to Phys. Rev. Lett. (October 14, 2005

Results for Greenland halibut, American plaice and Atlantic cod of the Spanish survey in NAFO Div. 3NO for the period 1997-2018

Greenland halibut (Reinhardtius hippoglossoides), American plaice (Hippoglossoides platessoides) and Atlantic cod (Gadus morhua) indices from the bottom trawl survey that Spain carries out in Spring since 1995 in Div. 3NO of the NAFO Regulatory Area are presented. Biomass, stratified mean catches and mean number per tow for the three species are presented since 1997, year in which the survey extended the depth strata. Mean catch per tow, length distribution and age distribution are presented for the last five years (2014-2018). Greenland halibut biomass and abundance estimates presented a decreasing trend since 1999, cut in 2007-2009 with a high increase. In 2011 the biomass drops under the 2008 value, being stable since then until 2014 with a slight increase in 2015-2018 comprising a big increase in 2017, reaching the maximum of the series. In last years it can be seen a presence of juveniles, mainly in 2004, but the greatest lengths have failed, although in 2009 there is a quite good presence of individuals of ages 6-7 and in 2010 between 5-7. In 2011-2018 the presence of all ages is poor, although in 2015-2016 an increase in the range of the length can be seen with regards to last years. For American plaice we can see a stable trend from 1999 to 2015, reaching a maximum of mean catch and number in 2006, and a severe decline since 2016. The greatest recruitment in the presented series occurred in 2004 and we can follow their mode along the years. No good recruitments were seen since then. The 2016 ALK for American plaice was not sexed. The 2017 and 2018 ALKs for American plaice are not available yet, so the 2016 ALK was used. In last years the level of all the ages is low. For Atlantic cod, it can be seen a low biomass until 2008, being higher and variable since then, reaching a historical maximum in 2014. From 2015, biomass decreased reaching in 2018 poor values at the level of the 2006 biomass. In 2007-2008 the youngest length classes were much over the rest of the length classes. With the 2006 cohort the series reaches the maximum number of its historical values at five years in 2011. There have been no good recruitments since 2009, although in 2015 and 2016 a discrete presence of individuals of age 1 can be seen

Estudio de la biocompatibilidad in vitro de formulaciones acrílicas autocurables portadoras de bisfosfonatos de nueva síntesis

En este trabajo se presenta el estudio de la biocompatibilidad in vitro, empleando cultivos celulares de fibroblastos embrionarios humanos, de tres sistemas acrílicos de liberación controlada de bisfosfonatos (BFs). Estos sistemas, basados en metacrilato de metilo (MMA), metacrilato de vitamina E (MVE) y trietilenglicol dimetacrilato (TEGDMA), han sido diseñados para su aplicación en el tratamiento de enfermedades caracterizadas por una elevada resorción osteoclástica. En su preparación se ha empleado un BP comercializado (alendronato; ALN) y dos de nueva síntesis, pertenecientes a la segunda y tercera generaciones, el ácido 1-hidroxi-2-[4-aminofenil]etano-1,1-difosfónico (APBP) y el ácido 1-hidroxi-2-[3-indolil]etano-1,1-difosfónico (IBP), respectivamente. La citotoxicidad de MVE ha sido notablemente inferior a la medida para TEGDMA. La citotoxicidad de APBP e IBP se ha comparado con la de ALN, obteniéndose unos valores para la concentración IC50, de15,56, 9,86 y 6,25 mmol/l para APBP, ALN e IBP, respectivamente. Los niveles de citotoxicidad liberada han sido superiores para la formulación portadora de ALN, situándose a continuación los cementos cargados con IBP y APBP. Cuando los cultivos se han establecido sobre la superficie de las formulaciones, se han detectado unos niveles de proliferación celular y adhesión superiores para los sistemas de liberación de IBP y APBP, siendo además inferior en estos casos la mortalidad celular.This paper reports the in vitro biocompatibility study, using human embryonic fibroblast cultures, of three acrylic systems for controlled release of bisphosphonates (BFs). These systems, that are based on methyl methacrylate (MMA), vitamin E methacrylate (MVE) and triethyleneglycol dimethacrylate (TEGDMA), have been designed for its application in the treatment of pathologies characterized by a high osteoclastic resorption. A commercial BP (alendronate; ALN) and two novel BPs, belonging to the second and third generations, 1-hydroxy-2-[4-aminophenyl]ethane-1,1-diphosphonic acid (APBP) and 1-hydroxy-2-[3-indolyl]ethane-1,1-diphosphonic acid (IBP), respectively, have been used in their preparation. The cytotoxicity of MVE has been notably lower than that measured for TEGDMA. Cytotoxicity of APBP and IBP was evaluated along with that of ALN, and the following values of the IC50, concentration were obtained for APBP, ALN and IBP respectively: 15.56, 9.86 and 6.25 mmol/l. The cytotoxicity levels obtained from the extracts of the cured systems were higher for the formulation containing ALN, followed by the cements loaded with IBP and APBP. When the cultures were seeded directly on the formulations surface, higher levels of cellular proliferation and adhesion were obtained for the systems containing IBP and APBP, the cellular mortality being also inferior in these cases