3,419 research outputs found
Performance of the EUDET-type beam telescopes
Test beam measurements at the test beam facilities of DESY have been
conducted to characterise the performance of the EUDET-type beam telescopes
originally developed within the EUDET project. The beam telescopes are equipped
with six sensor planes using MIMOSA26 monolithic active pixel devices. A
programmable Trigger Logic Unit provides trigger logic and time stamp
information on particle passage. Both data acquisition framework and offline
reconstruction software packages are available. User devices are easily
integrable into the data acquisition framework via predefined interfaces.
The biased residual distribution is studied as a function of the beam energy,
plane spacing and sensor threshold. Its standard deviation at the two centre
pixel planes using all six planes for tracking in a 6\,GeV
electron/positron-beam is measured to be
(2.88\,\pm\,0.08)\,\upmu\meter.Iterative track fits using the formalism of
General Broken Lines are performed to estimate the intrinsic resolution of the
individual pixel planes. The mean intrinsic resolution over the six sensors
used is found to be (3.24\,\pm\,0.09)\,\upmu\meter.With a 5\,GeV
electron/positron beam, the track resolution halfway between the two inner
pixel planes using an equidistant plane spacing of 20\,mm is estimated to
(1.83\,\pm\,0.03)\,\upmu\meter assuming the measured intrinsic resolution.
Towards lower beam energies the track resolution deteriorates due to increasing
multiple scattering. Threshold studies show an optimal working point of the
MIMOSA26 sensors at a sensor threshold of between five and six times their RMS
noise. Measurements at different plane spacings are used to calibrate the
amount of multiple scattering in the material traversed and allow for
corrections to the predicted angular scattering for electron beams
Dyadic Power Theory, Touch, and Counseling Psychology: A Response to Smith, Vogel, Madon, and Edwards (2011)
Smith, Vogel, Madon, and Edwardsâ (2011) recent article tested dyadic power theory (DPT) by examining the use of touch as a compliance-gaining tactic in the conflicts of married couples. In this response, we raise a methodological issue about the touch behaviors examined by Smith et al. and also pose a theoretical critique that their test of DPT violates an important scope condition of the theory. They did not examine differences between power-equal and power-unequal dyads, but instead they state that topic selection provides an actor with legitimate authority (and thus greater perceived power) and therefore the actor would touch their partner more to influence the partner. In contrast, DPT predicts that actors will use control attempts such as touch more when they are equal in power than when they are unequal. We believe DPT is relevant to touch in marital conflicts and provide a preliminary statement of that idea.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline
105PLONG-TERM EFFECTS OF EARLY PREGNANCY ON THE GENE EXPRESSION AND PROPERTIES OF MAMMARY EPITHELIAL CELL SUBPOPULATIONS IN MICE
From Teamchef Arminius to Hermann Junior: glocalised discourse about a national foundation myth
If for much of the nineteenth and twentieth centuries, the âBattle of the Teutoburg Forestâ, fought in 9 CE between Roman armies and Germanic tribes, was predominantly a reference point for nationalist and chauvinist discourses in Germany, the first decade of the twenty-first century has seen attempts to link public remembrance with local/regional identities on the one hand and international/intercultural contact on the other. In the run up to and during the âanniversary yearâ of 2009, German media, sports institutions and various other official institutions articulating tourist, economic and political interests attempted to create a new âglocalisedâ version of the public memory of the Teutoburg battle. Combining methods of Cognitive Linguistics and Critical Discourse Analysis, the paper analyses the narrative and argumentative topoi employed in this re-orientation of public memory, with a special emphasis on hybrid, post-national identity-construction. Das zweitausendjĂ€hrige Gedenkjahr der âSchlacht im Teutoburger Waldâ im Jahr 2009 bot eine gĂŒnstige Gelegenheit, die bis in die zweite HĂ€lfte des 20. Jahrhunderts dominante Tradition nationalistischâchauvinistischer Deutungen des Sieges von germanischen StĂ€mmen ĂŒber drei römische Legionen zu korrigieren und zu ĂŒberwinden. Der Aufsatz analysiert mit Hilfe diskurslinguistischer Methoden die Anstrengungen regionaler Institutionen und Medien, die nationale Vereinnahmung des historischen Gedenkens kritisch zu thematisieren sowie neue, zum eine lokal situierte, zum andern international orientierte Identifikationsangebote anzubieten. Die Analyse zeigt, dass solche âde-nationalisiertenâ Identifikationsangebote zwar teilweise auch frĂŒher verwendet wurden, aber heutzutage rekontextualisiert und auf innovative Weise in den Vordergrund gestellt werden
Controlled assembly of SNAP-PNA-fluorophore systems on DNA templates to produce fluorescence resonance energy transfer
The SNAP protein is a widely used self-labeling tag that can be used for tracking protein localization and trafficking in living systems. A model system providing controlled alignment of SNAP-tag units can provide a new way to study clustering of fusion proteins. In this work, fluorescent SNAP-PNA conjugates were controllably assembled on DNA frameworks forming dimers, trimers, and tetramers. Modification of peptide nucleic acid (PNA) with the O6-benzyl guanine (BG) group allowed the generation of site-selective covalent links between PNA and the SNAP protein. The modified BG-PNAs were labeled with fluorescent Atto dyes and subsequently chemo-selectively conjugated to SNAP protein. Efficient assembly into dimer and oligomer forms was verified via size exclusion chromatography (SEC), electrophoresis (SDS-PAGE), and fluorescence spectroscopy. DNA directed assembly of homo- and hetero-dimers of SNAP-PNA constructs induced homo- and hetero-FRET, respectively. Longer DNA scaffolds controllably aligned similar fluorescent SNAP-PNA constructs into higher oligomers exhibiting homo-FRET. The combined SEC and homo-FRET studies indicated the 1:1 and saturated assemblies of SNAP-PNA-fluorophore:DNA formed preferentially in this system. This suggested a kinetic/stoichiometric model of assembly rather than binomially distributed products. These BG-PNA-fluorophore building blocks allow facile introduction of fluorophores and/or assembly directing moieties onto any protein containing SNAP. Template directed assembly of PNA modified SNAP proteins may be used to investigate clustering behavior both with and without fluorescent labels which may find use in the study of assembly processes in cells
Study of the Epigenetic Signals in the Human Genome
Epigenetics can be defined as changes in the genome that are inherited during cell division, but without direct modification of the DNA sequence. These genomic changes are supported by three major epigenetic mechanisms: DNA methylation, histone modification and small RNAs. Different epigenetic marks function regulate gene transcription, some of them when altered can trigger various diseases such as cancer. This work is focus on the epigenetic signals in the human genome, studding the dependency between the nucleotide word context and the occurrence of epigenomic marking. We based our study on histone epigenomes available in the NIH Roadmap Epigenomics Mapping Consortium database that contains various types of cells and various types of tissues. We compared genomic contexts of epigenetic marking among chromosomes and among epigenomes. We included a control scenario, the DNA sequence regions without epigenetic marking. We identified significant differences between context occurrence of control and epigenetic regions. The genomic words in epigenetic marking regions present significant association with chromosome and histone modification type
- âŠ