Photodegradation of ciprofloxacin and levofloxacin by Au@ZnONPs-MoS2-Rgo nanocomposites

This study aimed to investigate the photocatalytic performance of diverse zinc oxide catalysts containing gold nanoparticles (AuNPs), molybdenum disulfide (MoS2), and reduced graphene oxide (rGO) toward the degradation of the antibiotics levofloxacin (LFX) and ciprofloxacin (CFX) in aqueous solutions. The obtained results demonstrate that LFX is more resistant to degradation when compared with CFX and that the principal route of degradation under visible light is the formation of hydroxyl radicals. Photoluminescence (PL) measurements were employed to verify the inhibitory effect of electron–hole recombination when AuNPs, MoS2, and rGO are integrated into a semiconductor. The catalyst that achieved the highest percentage of CFX degradation was 1%Au@ZnONPs-3%MoS2-1%rGO, exhibiting a degradation efficiency of 96%, while the catalyst that exhibited the highest percentage of LFX degradation was 5%Au@ZnONPs-3%MoS2-1%rGO, displaying a degradation efficiency of 99.8%. A gas chromatography–mass spectrometry (GC-MS) analysis enabled the identification of reaction intermediates, facilitating the determination of a potential degradation pathway for both antibiotics. Additionally, recyclability assessments showed that the synthesized catalysts maintained stable photocatalytic efficiencies after 15 cycles, indicating that the heterostructures have the potential for further usage and may be tested with other organic contaminants as wellThe financial support from the NSF Center for the Advancement of Wearable TechnologiesCAWT (grant 1849243), from the Consortium of Hybrid Resilient Energy Systems (DE-NA0003982), and from the Spanish Ministry of Economy and Competitiveness, under NanoCat-Com Project (PID2021-124667OB-I00), are gratefully acknowledgedThe financial support from the NSF Center for the Advancement of Wearable TechnologiesCAWT (grant 1849243), from the Consortium of Hybrid Resilient Energy Systems (DE-NA0003982), and from the Spanish Ministry of Economy and Competitiveness, under NanoCat-Com Project (PID2021-124667OB-I00), are gratefully acknowledge

Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers

Background: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and function of circulating neutrophils from children with COVID-19. Methods: An observational study including 182 children with COVID-19, 21 children with multisystem inflammatory syndrome (MIS-C), and 40 healthy children was performed in Buenos Aires, Argentina. Neutrophil phenotype was analyzed by flow cytometry in blood samples. Cytokine production, plasma levels of IgG antibodies directed to the spike protein of SARS-CoV-2 and citrullinated histone H3 were measured by ELISA. Cell-free DNA was quantified by fluorometry. Findings: Compared with healthy controls, neutrophils from children with COVID-19 showed a lower expression of CD11b, CD66b, and L-selectin but a higher expression of the activation markers HLA-DR, CD64 and PECAM-1 and the inhibitory receptors LAIR-1 and PD-L1. No differences in the production of cytokines and NETs were observed. Interestingly, the expression of CD64 in neutrophils and the serum concentration of IgG antibodies directed to the spike protein of SARS-CoV-2 distinguished asymptomatic from mild and moderate COVID-19. Interpretation: Acute lung injury is a prominent feature of severe COVID-19 in adults. A low expression of adhesion molecules together with a high expression of inhibitory receptors in neutrophils from children with COVID-19 might prevent tissue infiltration by neutrophils preserving lung function.Fil: Seery, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Raiden, Silvina Claudia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Algieri, Silvia C.. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Grisolía, Nicolás A.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Filippo, Daniela. Hospital Municipal Diego Thompson; ArgentinaFil: De Carli, Norberto. Clinica del Niño de Quilmes; ArgentinaFil: Di Lalla, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Cairoli, Héctor. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Chiolo, María J.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Meregalli, Claudia N.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Gimenez, Lorena I.. Hospital Municipal Diego Thompson; ArgentinaFil: Gregorio, Gabriela. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Sarli, Mariam. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Alcalde, Ana L.. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Davenport, Carolina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Bruera, María J.. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Simaz, Nancy. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Pérez, Mariela F.. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Nivela, Valeria. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Bayle, Carola. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Tuccillo, Patricia. Ministerio de Defensa. Armada Argentina. Hospital Naval Buenos Aires Cirujano Mayor Dr. Pedro Mallo; ArgentinaFil: Agosta, María T.. Ministerio de Defensa. Armada Argentina. Hospital Naval Buenos Aires Cirujano Mayor Dr. Pedro Mallo; ArgentinaFil: Pérez, Hernán. Ministerio de Defensa. Armada Argentina. Hospital Naval Buenos Aires Cirujano Mayor Dr. Pedro Mallo; ArgentinaFil: Villa Nova, Susana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Suárez, Patricia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Takata, Eugenia M.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: García, Mariela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Lattner, Jorge. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Rolón, María J.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Coll, Patricia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Sananez, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Holgado, María Pía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Ferrero, Fernando. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Arruvito, Maria Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentin

Antiprotozoan lead discovery by aligning dry and wet screening: Prediction, synthesis, and biological assay of novel quinoxalinones

Protozoan parasites have been one of the most significant public health problems for centuries and several human infections caused by them have massive global impact. Most of the current drugs used to treat these illnesses have been used for decades and have many limitations such as the emergence of drug resistance, severe side-effects, low-to-medium drug efficacy, administration routes, cost, etc. These drugs have been largely neglected as models for drug development because they are majorly used in countries with limited resources and as a consequence with scarce marketing possibilities. Nowadays, there is a pressing need to identify and develop new drug-based antiprotozoan therapies. In an effort to overcome this problem, the main purpose of this study is to develop a QSARs-based ensemble classifier for antiprotozoan drug-like entities from a heterogeneous compounds collection. Here, we use some of the TOMOCOMD-CARDD molecular descriptors and linear discriminant analysis (LDA) to derive individual linear classification functions in order to discriminate between antiprotozoan and non-antiprotozoan compounds as a way to enable the computational screening of virtual combinatorial datasets and/or drugs already approved. Firstly, we construct a wide-spectrum benchmark database comprising of 680 organic chemicals with great structural variability (254 of them antiprotozoan agents and 426 to drugs having other clinical uses). This series of compounds was processed by a k-means cluster analysis in order to design training and predicting sets. In total, seven discriminant functions were obtained, by using the whole set of atom-based linear indices. All the LDA-based QSAR models show accuracies above 85% in the training set and values of Matthews correlation coefficients (C) vary from 0.70 to 0.86. The external validation set shows rather-good global classifications of around 80% (92.05% for best equation). Later, we developed a multi-agent QSAR classification system, in which the individual QSAR outputs are the inputs of the aforementioned fusion approach. Finally, the fusion model was used for the identification of a novel generation of lead-like antiprotozoan compounds by using ligand-based virtual screening of 'available' small molecules (with synthetic feasibility) in our 'in-house' library. A new molecular subsystem (quinoxalinones) was then theoretically selected as a promising lead series, and its derivatives subsequently synthesized, structurally characterized, and experimentally assayed by using in vitro screening that took into consideration a battery of five parasite-based assays. The chemicals 11(12) and 16 are the most active (hits) against apicomplexa (sporozoa) and mastigophora (flagellata) subphylum parasites, respectively. Both compounds depicted good activity in every protozoan in vitro panel and they did not show unspecific cytotoxicity on the host cells. The described technical framework seems to be a promising QSAR-classifier tool for the molecular discovery and development of novel classes of broad - antiprotozoan - spectrum drugs, which may meet the dual challenges posed by drug-resistant parasites and the rapid progression of protozoan illnesses. © 2014 Elsevier Ltd. All rights reserved.Peer Reviewe

The New Neoliberal Turn in Argentina

Abstract This article examines reconfigurations of neoliberalism as these can be traced in emergent forms of ideological interpellation in Argentina. Privileging an analysis of dominant modes of public discourse, we posit an inextricable relationship between—and simultaneous deployment of—the “punitive” elements of this interpellation and its new, entrepreneurial dimension. This last component, opposed to the first only nominally, exalts individual potency and delivers an “amicable” appeal to a wholesome and reconciled common life. Taking into account the Janus-faced, omnipotent, moralizing, and sacrificial character of this new community of “entrepreneurs cum punishers,” we focus on the idiosyncratic forms that this relationship takes in our current historical conjuncture, distinguishing it from previous inflections and ideological configurations of neoliberalism in contemporary Argentina. Finally, we seek to understand the ethical and political implications of this specific interpellative mode for the process of subject formation.</jats:p

Polycyclic Aromatic Hydrocarbons (PAHs) in Freshwater Systems: A Comprehensive Review of Sources, Distribution, and Ecotoxicological Impacts

This comprehensive review offers new perspectives on the distribution, sources, and ecotoxicological impacts of polycyclic aromatic hydrocarbons (PAHs) in freshwater systems. Unlike previous reviews, this work integrates recent findings on PAH dynamics within environmental matrices and emphasizes spatiotemporal variability across geographic regions. It critically examines both anthropogenic and natural sources, as well as the physical, chemical, and biological mechanisms driving PAH transport and fate. Special attention is given to the ecotoxicological effects of PAHs on freshwater organisms, including bioaccumulation, endocrine disruption, and genotoxicity. Notably, this review identifies key knowledge gaps and proposes an interdisciplinary framework to assess ecological risk and guide effective monitoring and management strategies for the protection of freshwater ecosystems

Hemophagocytic lymphohistiocytosis secondary to AIDS-related histoplasmosis and visceral leishmaniasis

Hemophagocytic Lymphohistiocytosis (HLH) is a rare, highly aggressive syndrome involving dysregulated immune response. Most cases are secondary to underlying diseases including infections, autoimmune disorders and malignancies. The burden of disease of histoplasmosis and leishmaniosis associated with advanced HIV is still significant in low-and-middle income countries (LMIC). We present a case of histoplasmosis and leishmaniasis associated HLH in a man with an AIDS diagnosis. </jats:p

Caregivers’ worries about type 1 diabetes in adolescent offspring: Examining experiences from hispanic parents

Caregivers of youth with type 1 diabetes (T1D) are continuously worried about their child’s health. Using a mixed-method strategy while conducting a secondary analysis of cross-sectional data, we examined the content of caregivers’ worries about T1D in adolescents (aged 12-17) and the relationship between multiple worries (MW) and health- and family-related variables (HFRV). We screened 65 Hispanic caregivers (81.54% women; aged 32–58) for a youth depression-treatment study. Caregivers answered an open-ended question regarding their worries about their child’s T1D. We identified the main themes of parental worries and coded responses using content-based categories. Using chi-square tests (p ≤ .05) and Spearman correlation, we assessed associations between MW and HFRV. Categories of worries and their occurrence were: Comorbidity and Future Functionality (CFF; 41.51%), Psychological/Cognitive Aspects of Adolescents (10.38%), Issues About Self-Care (16.98%), Short Term Complications/Emergency Situations (19.81%), Repercussions on Families/Caregivers (9.43%), and Discrimination/Lack of Sensitivity (1.89%). MW (≥2) were associated with lower adherence to exercise, higher psychotropic medication use among adolescents, and lower family expressiveness and cohesion. Caregivers with concerns about CFF had children with higher rates of poor glycemic control and fewer hypoglycemia preventive behaviors. Our findings suggest that diabetes complications and adherence to self-care, are the main themes of caregivers’ worries and that MW significantly relate to relevant HFRV.Quienes cuidan jóvenes con diabetes tipo 1 (DT1) sienten preocupación continua por su salud. Utilizando una estrategia de métodos mixtos al realizar un análisis secundario de datos transversales, examinamos el contenido de las preocupaciones parentales sobre la DT1 en adolescentes (12-17 años) y la relación entre preocupaciones múltiples (PM) y variables familiares y sobre la salud (VFSS). Evaluamos 65 cuidadoras/es (81.54% mujeres; 32–58 años) durante un estudio para tratar la depresión juvenil. Respondieron una pregunta abierta sobre sus preocupaciones con la DT1 en jóvenes. Identificamos los temas principales y codificamos las respuestas con categorías basadas en contenido. Usando chi-cuadrado y correlación de Spearman, evaluamos la asociación entre PM y VFSS. Las categorías y su frecuencia fueron: Comorbilidad/Funcionalidad Futura (CFF; 41.51%), Aspectos Psicológicos/Cognitivos en Adolescentes (10.38%), Asuntos Sobre Autocuidado (16.98%), Complicaciones a Corto Plazo/Emergencias (19.81%), Repercusiones en la Familia/Cuidadoras(es) (9.43%) y Discriminación/Falta de Sensibilidad (1.89%). Las PM (≥2) se asociaron con menor adherencia al ejercicio, mayor uso de psicotrópicos y menor expresividad y cohesión familiar. Quienes tuvieron preocupaciones sobre CFF tenían hijos/as con tasas mayores de descontrol glucémico y menos prevención hipoglucémica. Nuestros hallazgos sugieren que las complicaciones diabéticas y el cumplimiento con el autocuidado son las principales preocupaciones parentales sobre la DT1 en adolescentes y que tener PM se vincula con VFSS relevantes

Validation of a Lateral Flow Assay for Rapid Diagnosis of Histoplasmosis in Advanced HIV Disease, Buenos Aires, Argentina

Histoplasmosis is a major cause of mortality in individuals with advanced human immunodeficiency virus (HIV) disease (AHD). We evaluated in patients with AHD a lateral flow assay (LFA) developed by MiraVista&reg; Diagnostics (MVD LFA). Histoplasmosis was defined based on the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) case definitions. We also compared the results of this LFA with those obtained using a commercial enzyme immunoassay (EIA) developed by IMMY, Clarus Histoplasma GM EIA, IMMY (HGM EIA). A retrospective observational study was conducted at Hospital Juan A. Fern&aacute;ndez, located in Buenos Aires, Argentina. The study included 48 urine specimens from patients aged &gt;18 years with AHD. Urine specimens included 17 patients with disseminated histoplasmosis and 31 specimens from patients without evidence of histoplasmosis. Specimens were tested using the MVD LFA and the HGM EIA. The MVD LFA and the HGM EIA had similar analytical performance, with a sensitivity of 94%, specificity of 100%, positive predictive value of 100%, negative predictive value of 97%, and an accuracy of 98%. Comparison of the MVD LFA with the HGM EIA demonstrated a Kappa agreement index of 0.906. The LFA evaluated in this study had high analytical performance; it provided rapid diagnosis of histoplasmosis with minimal requirements for laboratory training, equipment, and laboratory infrastructure

33-Year Contribution of the Cytogenetics and Human Genetics Laboratory (LACyGH) – UNaM – IPS Agreement

In 1975, Professor Mario Rubén Beltrami proposed the creation of a “Human Cytogenetics Laboratory” with the aim of “promoting, teaching, and researching human genetics and cytogenetics.” This is how the Laboratory of Cytogenetics and Human Genetics (LACyGH) began and was founded, through the first agreement signed in 1976 between the Higher Institute of Teachers of the National University of Misiones (UNaM) and the provincial Ministry of Public Health. In its initial phase, LACyGH operated in the Pathology Pavilion of Dr. Ramón Madariaga Hospital. Later, in the 1980s, it moved to the facilities of the Clinical Analysis Laboratory of the Institute of Social Security of the province of Misiones, carrying out the training of human resources. Since 1991, cytogenetic services to members of the provincial health affiliates and hospital patients are provided. Over time, it incorporated additional services, infrastructure improvements, and equipment upgrades, which made it become a pioneer, collaborating with the teaching work in genetics degree program and connected UNaM with the community through genetic services, promoting the initial training of professionals and disseminating this discipline by engaging with the local and regional community. Initially, LACyGH primarily processed peripheral blood samples (PB), which was related to technical feasibility, ease of sample collection, the growing awareness of the utility of cytogenetic studies, and medical training. The second type of material introduced was spontaneous abortion material (SAM), reflecting changes in medical practices related to the management of miscarriage cases. Subsequently, LACyGH began conducting studies on bone marrow (BM) and lymph nodes (LN), highlighting the integration of chromosomal data as an essential tool in the diagnosis, prognosis, and treatment of oncohematological diseases. From 1990 to 2022, the LACyGH received and processed samples with the aim of establishing the karyotype, which made it possible to globally visualize the genome and identify chromosomal alterations, understand the causes and mechanisms that cause them, and provide prognostic information and therapeutic selection. rational. Of a total of 5,649 samples analyzed, 3,048 cases were male and 2,601 cases were female. Regarding the type of biological material, 3976 SP samples, 1479 MO samples, 163 MAE samples and 31 GL samples were processed. Regarding cytogenetic results, 3908 normal karyotypes, 978 karyotypes with chromosomal abnormalities, and 763 studies without cell growth were obtained. These data serve to rethink and propose solutions to the inconveniences in obtaining the material, supplies and times, in order to obtain better preparations and results, increasing the efficiency of the service.Durante 1975, el Profesor Mario Rubén Beltrami propone la creación de un “Laboratorio de Citogenética Humana” que tendría por objeto la “difusión, enseñanza e investigación de la genética y citogenética humana”. Es así que como se inicia y es fundado el Laboratorio de Citogenética y Genética Humana (LACyGH), mediante el primer convenio suscripto en 1976 entre el Instituto Superior del Profesorado de la Universidad Nacional de Misiones (UNaM) y el Ministerio de Salud Pública provincial. En una primera etapa, el LACyGH funcionó en el Pabellón de Patología del Hospital Dr. Ramón Madariaga. Con posteridad, en la década de 1980, pasa a las instalaciones del Laboratorio de Análisis Clínicos del Instituto de Previsión Social de la provincia de Misiones, cumpliendo con la formación de recursos humanos. A partir del año 1991, comienza la prestación de servicios citogenéticos a los afiliados a la obra social provincial y a los pacientes hospitalarios. Progresivamente fue incorporando prestaciones, mejoras en infraestructura y equipamientos, que hicieron que se constituya como pionero, colaborando con la labor docente de la Licenciatura en Genética y vinculando a la UNaM con la sociedad a través de la prestación de servicios de genética, promoviendo la formación inicial de profesionales y contribuyendo con la divulgación de esta disciplina, articulando acciones con la comunidad local y regional. El LACyGH comenzó procesando de manera casi exclusiva muestras de sangre periférica (SP), lo cual estuvo relacionado con las posibilidades técnicas, la mayor factibilidad y menor rigurosidad de dicho material, la difusión de la utilidad de los estudios citogenéticos y la formación de los médicos en actividad. El segundo material incorporado fue de material de aborto espontáneo (MAE), relacionado con el cambio en la conducta médica respecto al manejo de los casos de abortos espontáneos. Posteriormente, comenzó con estudios en médula ósea (MO), y en ganglios linfáticos (GL), lo cual refleja la incorporación al diagnóstico clínico de los datos cromosómicos como herramienta indispensable en la definición, pronóstico y tratamiento de las enfermedades oncohematológicas. Desde 1990 hasta 2022, el LACyGH recibió y procesó muestras con el objetivo de establecer el cariotipo, lo cual permitió visualizar en forma global el genoma e identificar alteraciones cromosómicas, comprender las causas y mecanismos que las provocan, y brindar información pronóstica y una selección terapéutica racional. De un total de 5649 muestras analizadas, 3048 casos resultaron de sexo masculino y 2601 casos de sexo femenino. Respecto al tipo de material biológico, se procesaron 3976 muestras de SP, 1479 muestras de MO, 163 muestras de MAE y 31 muestras de GL. En cuanto a los resultados citogenéticos, se obtuvieron 3908 cariotipos normales, 978 cariotipos con anomalías cromosómicas, y 763 estudios sin crecimiento celular. Sirvan estos datos para repensar y plantear soluciones sobre los inconvenientes tanto en la obtención del material, insumos y tiempos, a fin de obtener mejores preparados y resultados, aumentando la eficiencia del servicio

Primer aislamiento en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina

ResumenDescribimos el primer caso en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina.Caso clínico: Paciente masculino, con antecedente de insuficiencia renal crónica en hemodiálisis y osteosíntesis de cadera debido a una fractura. Se internó por síndrome febril persistente luego del desplazamiento de la prótesis por un traumatismo. Se aisló de hemocultivos S. aureus resistente a la meticilina adquirido en la comunidad. Durante el tratamiento con vancomicina y daptomicina, se observó un aumento gradual de la CIM de vancomicina de 1μg/ml (VSSA) a 2μg/ml (h-VISA) y a 4μg/ml (VISA), y la emergencia de no sensibilidad a daptomicina (CIM=4μg/ml). Al suspender la vancomicina y la daptomicina, la cepa revirtió al fenotipo de sensibilidad a ambas drogas. Es mandatorio evaluar por CIM la sensibilidad a vancomicina y a daptomicina intratratamiento cuando estas drogas se usan como terapia.AbstractWe report the first case in Argentina of community-acquired methicillin-resistant Staphylococcus aureus with intermediate susceptibility to vancomycin and nonsusceptibility to daptomycin.Case report: A male patient with a history of chronic renal failure on hemodialysis and hip fracture osteosynthesis was admitted to hospital for persistent febrile syndrome following the displacement of the prosthesis by trauma. Blood cultures grew communityacquired methicillin-resistant Staphylococcus aureus. During treatment with vancomycin and daptomycin, a gradual increase in vancomycin MIC of 1 μg/ml (VSSA) to 2μg/ml (h-VISA) and 4μg/ml (VISA) was observed, as well as the emergence of non-susceptibility to daptomycin (MIC=4μg/ml). By suspending vancomycin and daptomycin, the strain reversed to the susceptible phenotype to both drugs. It is mandatory to evaluate by MIC the susceptibility to vancomycin and daptomycin during treatment when these drugs are used as therapy