Genome Features of “Dark-Fly”, a Drosophila Line Reared Long-Term in a Dark Environment

Organisms are remarkably adapted to diverse environments by specialized metabolisms, morphology, or behaviors. To address the molecular mechanisms underlying environmental adaptation, we have utilized a Drosophila melanogaster line, termed “Dark-fly”, which has been maintained in constant dark conditions for 57 years (1400 generations). We found that Dark-fly exhibited higher fecundity in dark than in light conditions, indicating that Dark-fly possesses some traits advantageous in darkness. Using next-generation sequencing technology, we determined the whole genome sequence of Dark-fly and identified approximately 220,000 single nucleotide polymorphisms (SNPs) and 4,700 insertions or deletions (InDels) in the Dark-fly genome compared to the genome of the Oregon-R-S strain, a control strain. 1.8% of SNPs were classified as non-synonymous SNPs (nsSNPs: i.e., they alter the amino acid sequence of gene products). Among them, we detected 28 nonsense mutations (i.e., they produce a stop codon in the protein sequence) in the Dark-fly genome. These included genes encoding an olfactory receptor and a light receptor. We also searched runs of homozygosity (ROH) regions as putative regions selected during the population history, and found 21 ROH regions in the Dark-fly genome. We identified 241 genes carrying nsSNPs or InDels in the ROH regions. These include a cluster of alpha-esterase genes that are involved in detoxification processes. Furthermore, analysis of structural variants in the Dark-fly genome showed the deletion of a gene related to fatty acid metabolism. Our results revealed unique features of the Dark-fly genome and provided a list of potential candidate genes involved in environmental adaptation

Primary Stability in Cementless Rotating Platform Total Knee Arthroplasty

Highly porous ingrowth surfaces have been introduced into tibial tray fixation to improve long-term survivorship in cementless total knee arthroplasty. This study was designed to evaluate the effect of porous ingrowth surface on primary stability in the implanted cementless tibial component. Three tibial tray designs possessing sintered bead or roughened porous coating ingrowth surfaces were implanted into a foam tibia model with primary stability assessed via digital image correlation during stair descent and condylar liftoff loading. Follow-up testing was conducted by implanting matched-pair cadaveric tibias with otherwise identical trays with two iterations of ingrowth surface design. Trays were loaded and micromotion evaluated in a condylar liftoff model. The sintered bead tibial tray exhibited slightly lower micromotion than the roughened porous coating in stair descent loading. However, no significant difference in primary stability was observed in condylar liftoff loading in either foam or cadaveric specimens. Cementless tibial trays featuring two different iterations of porous ingrowth surfaces demonstrated both good stability in cadaveric specimens with less than 80 microns of micromotion and 1 mm of subsidence under cyclic loading. While improved ingrowth surfaces may lead to improved biological fixation and long-term osteointegration, this study was unable to identify a difference in primary stability associated with subsequent ingrown surface design iteration.</jats:p

Digital image correlation techniques for strain measurement in a variety of biomechanical test models

Purpose: Previous biomechanical studies have estimated the strains of bone and bone substitutes using strain gages. However, applying strain gages to biological samples can be difficult, and data collection is limited to a small area under the strain gage. The purpose of this study was to compare digital image correlation (DIC) strain measurements to those obtained from strain gages in order to assess the applicability of DIC technology to common biomechanical testing scenarios. Methods: Compression and bending tests were conducted on aluminum alloy, polyurethane foam, and laminated polyurethane foam specimens. Simplified single-legged stance loads were applied to composite and cadaveric femurs. Results: Results showed no significant differences in principal strain values (or variances) between strain gage and DIC measurements on the aluminum alloy and laminated polyurethane foam specimens. There were significant differences between the principal strain measurements of the non-laminated polyurethane foam specimens, but the deviation from theoretical results was similar for both measurement techniques. DIC and strain gage data matched well in 83.3% of all measurements in composite femur models and in 58.3% of data points in cadaveric specimens. Increased variation in cadaveric data was expected, and is associated with the well-documented variability of strain gage analysis on hard tissues as a function of bone temperature, hydration, gage protection, and other factors specific to cadaveric biomechanical testing. Conclusions: DIC techniques provide similar results to those obtained from strain gages across standard and anatomical specimens while providing the advantages of reduced specimen preparation time and full-field data analysis.</p

Do conflict resolution and recovery predict the survival of adolescents' romantic relationships?

Contains fulltext : 116456.pdf (publisher's version ) (Open Access)Numerous studies have shown that being able to resolve and recover from conflicts is of key importance for relationship satisfaction and stability in adults. Less is known about the importance of these relationship dynamics in adolescent romantic relationships. Therefore, this study investigated whether conflict resolution and recovery predict breakups in middle adolescent couples. Couples who are able to resolve and recover from conflict were expected to demonstrate a lower probability of breaking up. In total, 80 adolescent couples (M age = 15.48, SD = 1.16) participated in a 4-wave prospective questionnaire and observational study, with one year between measurements. In addition to self-report measures, adolescents were observed in real-time during conflicts with their partners. Multilevel Proportional Hazard analyses revealed that, contrary to the hypothesis, conflict resolution and conflict recovery did not predict the likelihood of breakup. Survival differences were not attributable to conflict resolution or conflict recovery. More research is needed to consider the unique relationship factors of adolescent romantic relationships to determine why some relationships survive while others do not.6 p

Process forecasting: towards proactive business process management

© Springer Nature Switzerland AG 2018. The digital economy is highly volatile and uncertain. Ever-changing customer needs and technical progress increase the pressure on organizations to continuously improve and innovate their business processes. The ability to anticipate incremental and radical process changes required in the future is a critical success factor. However, organizations often fail to forecast future business process designs and process performance. One reason is that Business Process Management (BPM) is dominated by reactive methods (e.g., lean management, traditional process monitoring), whereas there are only a few future-oriented approaches (e.g., process simulation, predictive process monitoring). This paper supports the shift towards proactive BPM by coining the notion of process forecasting – an umbrella concept for future-oriented BPM methods and techniques. We motivate the need for process forecasting by eliciting various types of process forecasting from BPM use cases and create a first understanding of its scope by providing a definition, a reference process, showing the steps to be followed in process forecasting initiatives, and a positioning against related BPM sub-areas. The definition and reference process are based on a structured literature review