Mechanical properties and cytotoxic evaluation of the Ti-3Nb-13Zr alloy

Ti-13Nb-13Zr is a new titanium alloy that was originally developed for medical implant applications. This alloy combines a low elastic modulus, high strength, excellent hot and cold workability, and superior corrosion resistance. Research on this alloy has shown that the mechanical properties can be controlled over a significant range through hot working, heat treatment and cold-working. The present study describes the mechanical properties and cytotoxic evaluation of the Ti-13Nb-13Zr alloy, which was produced by furnace arc melting in argon atmosphere. The elemental constituents were unalloyed Ti, Nb and Zr sheets. The obtained ingots, which initial diameter were about 15 mm, have undergone sequences of cold-working and heat treatments in order to achieve a final diameter of 6 mm. The tensile strength of Ti-13Nb-13Zr achieved 1270 MPa (cold-worked – 60% reduction in area) and 860 MPa after heat treatment (60% reduction in area + 1000 o C/1h + water quenched). The elastic module were 52 GPa and 60 GPa respectively. Furthermore, the toxic effect of this alloy on cells was evaluated by a cytotoxicity test, a quantitative method of colony suppression assay using Chinese Hamster Ovary (CHO) cultured cells in contact with diluted extracts of the biomaterials. The results showed that Ti-13Nb-13Zr alloy obtained by furnace arc melting isn’t cytotoxic.Peer Reviewe

Mechanical properties and cytotoxic evaluation of the Ti-3Nb-13Zr alloy

Ti-13Nb-13Zr is a new titanium alloy that was originally developed for medical implant applications. This alloy combines a low elastic modulus, high strength, excellent hot and cold workability, and superior corrosion resistance. Research on this alloy has shown that the mechanical properties can be controlled over a significant range through hot working, heat treatment and cold-working. The present study describes the mechanical properties and cytotoxic evaluation of the Ti-13Nb-13Zr alloy, which was produced by furnace arc melting in argon atmosphere. The elemental constituents were unalloyed Ti, Nb and Zr sheets. The obtained ingots, which initial diameter were about 15 mm, have undergone sequences of cold-working and heat treatments in order to achieve a final diameter of 6 mm. The tensile strength of Ti-13Nb-13Zr achieved 1270 MPa (cold-worked – 60% reduction in area) and 860 MPa after heat treatment (60% reduction in area + 1000 o C/1h + water quenched). The elastic module were 52 GPa and 60 GPa respectively. Furthermore, the toxic effect of this alloy on cells was evaluated by a cytotoxicity test, a quantitative method of colony suppression assay using Chinese Hamster Ovary (CHO) cultured cells in contact with diluted extracts of the biomaterials. The results showed that Ti-13Nb-13Zr alloy obtained by furnace arc melting isn’t cytotoxic.Peer Reviewe

An electrochemical study of the behaviour of ear piercing studs immersed in a culture medium

Two commercial studs, of gold-coated stainless steel and copper-zinc alloy, respectively, and a laboratory-made titanium stud were chosen for corrosion studies. Corrosion behaviour in a culture medium (CM) was studied using electrochemical impedance spectroscopy and polarization measurements, as a function of immersion time. The elements that leached out into the CM electrolyte were analysed by instrumental neutron activation analysis. Scanning electron microscopy and energy dispersive X-ray techniques were utilized in the analysis of the stud surfaces before and after their immersion in a CM solution. The cytotoxicity of the tested studs was also determined. The titanium stud showed the best combination of properties, high corrosion resistance and tow cytotoxicity, while the gold-coated stainless steel stud occupied an intermediate position.Peer Reviewe

α-tricalcium Phosphate Cement: "in Vitro" Cytotoxicity

Calcium phosphate-based bioceramics have revolutionized orthopedic and dental repair of damaged parts of the bone system. Among these materials, calcium phosphate-based cements, with hydraulic setting, stand out due to their biocompatibility and in situ hardening, which allow easy manipulation and adaptation to the shape and dimensions of bone defects. An investigation was made of the in vitro cytotoxic effect of calcium phosphate cement based on α-tricalcium phosphate, immersed for different lengths of time in simulated body fluid (SBF), based on the ISO-10993 "Biological Evaluation of Medical Devices" standard. The culture medium was Chinese hamster ovary (CHO) cells in contact with diluted cement extracts. The results revealed that the calcium phosphate cement used was cytotoxic and that the material's cytotoxicity decreased the longer the cement was immersed in SBF. © 2002 Elsevier Science Ltd. 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