5,976 research outputs found
Acquired potential N-glycosylation sites within the tumor-specific immunoglobulin heavy chains of B-cell malignancies
Background and Objectives. Among B-cell malignancies, follicular lymphomas (FL)
more frequently show acquired, potential N-glycosylation sites (AGS) within tumor-specific
immunoglobulin. The aim of this study was to extend this observation and to evaluate
the pattern of presentation of AGS within five different forms of B-cell lymphoma.
Design and Methods. We sequenced the tumor-specific immunoglobulin heavy chain
variable region fragment, including complementarity-determining regions 2 and 3, of
forty-seven consecutive patients with a B-cell malignancy enrolled in idiotype vaccine
clinical trials. This sequencing approach is known to allow the identification of most AGS.
We then statistically analyzed differences in presentation pattern, in terms of tumor histology,
immunoglobulin isotype, AGS location and amino acid composition.
Results. All twenty-four FL cases presented with at least one AGS, whereas the vast
majority of four B-cell lymphoma types other than FL did not. The non- FL group of tumors
included four cases of Burkittâs lymphoma, six of diffuse large cell lymphoma, seven mantle
cell lymphomas and six small lymphocytic lymphomas. Most IgM-bearing follicular
lymphoma cases featured their AGS within complementarity-determining region 2, as
opposed to those bearing an IgG, which mostly displayed the AGS within complementarity-
determining region 3. The vast majority of AGS located within either complementarity-
determining region ended with a serine residue, whereas those located within framework
regions mostly featured threonine as the last amino acid residue.
Interpretation and Conclusions. In our series, all cases of FL had AGS within their
tumor-specific immunoglobulin heavy chain variable regions. In contrast, most B-cell
malignancies other than FL did not. Further studies are warranted in order to establish
the possible meaning of these findings in terms of disease pathogenesis, their diagnostic
value in doubtful cases and their potential implications for immunotherapy
Non-parametric deprojection of NIKA SZ observations: Pressure distribution in the Planck-discovered cluster PSZ1 G045.85+57.71
The determination of the thermodynamic properties of clusters of galaxies at
intermediate and high redshift can bring new insights into the formation of
large-scale structures. It is essential for a robust calibration of the
mass-observable scaling relations and their scatter, which are key ingredients
for precise cosmology using cluster statistics. Here we illustrate an
application of high resolution arcsec) thermal Sunyaev-Zel'dovich (tSZ)
observations by probing the intracluster medium (ICM) of the \planck-discovered
galaxy cluster \psz\ at redshift , using tSZ data obtained with the
NIKA camera, which is a dual-band (150 and 260~GHz) instrument operated at the
IRAM 30-meter telescope. We deproject jointly NIKA and \planck\ data to extract
the electronic pressure distribution from the cluster core () to its outskirts () non-parametrically for the
first time at intermediate redshift. The constraints on the resulting pressure
profile allow us to reduce the relative uncertainty on the integrated Compton
parameter by a factor of two compared to the \planck\ value. Combining the tSZ
data and the deprojected electronic density profile from \xmm\ allows us to
undertake a hydrostatic mass analysis, for which we study the impact of a
spherical model assumption on the total mass estimate. We also investigate the
radial temperature and entropy distributions. These data indicate that \psz\ is
a massive ( M) cool-core cluster.
This work is part of a pilot study aiming at optimizing the treatment of the
NIKA2 tSZ large program dedicated to the follow-up of SZ-discovered clusters at
intermediate and high redshifts. (abridged)Comment: 16 pages, 10 figure
Prognostic ability of EndoPredict compared to research-based versions of the PAM50 risk of recurrence (ROR) scores in node-positive, estrogen receptor-positive, and HER2-negative breast cancer. A GEICAM/9906 sub-study
There are several prognostic multigene-based tests for managing breast cancer (BC), but limited data comparing them in the same cohort. We compared the prognostic performance of the EndoPredict (EP) test (standardized for pathology laboratory) with the research-based PAM50 non-standardized qRT-PCR assay in node-positive estrogen receptor-positive (ER+) and HER2-negative (HER2â) BC patients receiving adjuvant chemotherapy followed by endocrine therapy (ET) in the GEICAM/9906 trial. EP and PAM50 risk of recurrence (ROR) scores [based on subtype (ROR-S) and on subtype and proliferation (ROR-P)] were compared in 536 ER+/HER2â patients. Scores combined with clinical information were evaluated: ROR-T (ROR-S, tumor size), ROR-PT (ROR-P, tumor size), and EPclin (EP, tumor size, nodal status). Patients were assigned to risk-categories according to prespecified cutoffs. Distant metastasis-free survival (MFS) was analyzed by KaplanâMeier. ROR-S, ROR-P, and EP scores identified a low-risk group with a relative better outcome (10-year MFS: ROR-S 87%; ROR-P 89%; EP 93%). There was no significant difference between tests. Predictors including clinical information showed superior prognostic performance compared to molecular scores alone (10-year MFS, low-risk group: ROR-T 88%; ROR-PT 92%; EPclin 100%). The EPclin-based risk stratification achieved a significantly improved prediction of MFS compared to ROR-T, but not ROR-PT. All signatures added prognostic information to common clinical parameters. EPclin provided independent prognostic information beyond ROR-T and ROR-PT. ROR and EP can reliably predict risk of distant metastasis in node-positive ER+/HER2â BC patients treated with chemotherapy and ET. Addition of clinical parameters into risk scores improves their prognostic ability.Electronic supplementary materialThe online version of this article (doi:10.1007/s10549-016-3725-z) contains supplementary material, which is available to authorized users
NIKA 2: next-generation continuum/polarized camera at the IRAM 30 m telescope and its prototype
NIKA 2 (New Instrument of Kids Array) is a next generation continuum and
polarized instrument successfully installed in October 2015 at the IRAM 30 m
telescope on Pico-Veleta (Granada, Spain). NIKA 2 is a high resolution
dual-band camera, operating with frequency multiplexed LEKIDs (Lumped Element
Kinetic Inductance Detectors) cooled at 100 mK. Dual color images are obtained
thanks to the simultaneous readout of a 1020 pixels array at 2 mm and 1140 x 2
pixels arrays at 1.15 mm with a final resolution of 18 and 12 arcsec
respectively, and 6.5 arcmin of Field of View (FoV). The two arrays at 1.15 mm
allow us to measure the linear polarization of the incoming light. This will
place NIKA 2 as an instrument of choice to study the role of magnetic fields in
the star formation process. The NIKA experiment, a prototype for NIKA 2 with a
reduced number of detectors (about 400 LEKIDs) and FoV (1.8 arcmin), has been
successfully operated at the IRAM 30 telescope in several open observational
campaigns. The performance of the NIKA 2 polarization setup has been
successfully validated with the NIKA prototype.Comment: 5 pages, 4 figures, proceeding for the conference: Extragalactic
radio surveys 201
Mapping the kinetic Sunyaev-Zel'dovich effect toward MACS J0717.5+3745 with NIKA
Measurement of the gas velocity distribution in galaxy clusters provides
insight into the physics of mergers, through which large scale structures form
in the Universe. Velocity estimates within the intracluster medium (ICM) can be
obtained via the Sunyaev-Zel'dovich (SZ) effect, but its observation is
challenging both in term of sensitivity requirement and control of systematic
effects, including the removal of contaminants. In this paper we report
resolved observations, at 150 and 260 GHz, of the SZ effect toward the triple
merger MACS J0717.5+3745 (z=0.55), using data obtained with the NIKA camera at
the IRAM 30m telescope. Assuming that the SZ signal is the sum of a thermal
(tSZ) and a kinetic (kSZ) component and by combining the two NIKA bands, we
extract for the first time a resolved map of the kSZ signal in a cluster. The
kSZ signal is dominated by a dipolar structure that peaks at -5.1 and +3.4
sigma, corresponding to two subclusters moving respectively away and toward us
and coincident with the cold dense X-ray core and a hot region undergoing a
major merging event. We model the gas electron density and line-of-sight
velocity of MACS J0717.5+3745 as four subclusters. Combining NIKA data with
X-ray observations from XMM-Newton and Chandra, we fit this model to constrain
the gas line-of-sight velocity of each component, and we also derive, for the
first time, a velocity map from kSZ data (i.e. that is model-dependent). Our
results are consistent with previous constraints on the merger velocities, and
thanks to the high angular resolution of our data, we are able to resolve the
structure of the gas velocity. Finally, we investigate possible contamination
and systematic effects with a special care given to radio and submillimeter
galaxies. Among the sources that we detect with NIKA, we find one which is
likely to be a high redshift lensed submillimeter galaxy.Comment: 19 pages, 9 figures, accepted in A&
High-resolution tSZ cartography of clusters of galaxies with NIKA at the IRAM 30-m telescope
The thermal Sunyaev-Zeldovich effect (tSZ) is a powerful probe to study
clusters of galaxies and is complementary with respect to X-ray, lensing or
optical observations. Previous arcmin resolution tSZ observations ({\it e.g.}
SPT, ACT and Planck) only enabled detailed studies of the intra-cluster medium
morphology for low redshift clusters (). Thus, the development of
precision cosmology with clusters requires high angular resolution observations
to extend the understanding of galaxy cluster towards high redshift. NIKA2 is a
wide-field (6.5 arcmin field of view) dual-band camera, operated at and containing KID (Kinetic Inductance Detectors), designed to
observe the millimeter sky at 150 and 260 GHz, with an angular resolution of 18
and 12 arcsec respectively. The NIKA2 camera has been installed on the IRAM
30-m telescope (Pico Veleta, Spain) in September 2015. The NIKA2 tSZ
observation program will allow us to observe a large sample of clusters (50) at
redshift ranging between 0.5 and 1. As a pathfinder for NIKA2, several clusters
of galaxies have been observed at the IRAM 30-m telescope with the NIKA
prototype to cover the various configurations and observation conditions
expected for NIKA2.Comment: Proceedings of the 28th Texas Symposium on Relativistic Astrophysics,
Geneva, Switzerland, December 13-18, 201
The NIKA instrument: results and perspectives towards a permanent KID based camera for the Pico Veleta observatory
The New IRAM KIDs Array (NIKA) is a pathfinder instrument devoted to
millimetric astronomy. In 2009 it was the first multiplexed KID camera on the
sky; currently it is installed at the focal plane of the IRAM 30-meters
telescope at Pico Veleta (Spain). We present preliminary data from the last
observational run and the ongoing developments devoted to the next NIKA-2
kilopixels camera, to be commissioned in 2015. We also report on the latest
laboratory measurements, and recent improvements in detector cosmetics and
read-out electronics. Furthermore, we describe a new acquisition strategy
allowing us to improve the photometric accuracy, and the related automatic
tuning procedure.Comment: 24th International Symposium on Space Terahertz Technology, ISSTT
2013, April 8 to 10, 2013, Groningen, the Netherland
High angular resolution Sunyaev-Zel'dovich observations of MACS J1423.8+2404 with NIKA: Multiwavelength analysis
The prototype of the NIKA2 camera, NIKA, is an instrument operating at the
IRAM 30-m telescope, which can observe simultaneously at 150 and 260GHz. One of
the main goals of NIKA2 is to measure the pressure distribution in galaxy
clusters at high resolution using the thermal SZ (tSZ) effect. Such
observations have already proved to be an excellent probe of cluster pressure
distributions even at high redshifts. However, an important fraction of
clusters host submm and/or radio point sources, which can significantly affect
the reconstructed signal. Here we report on <20" resolution observations at 150
and 260GHz of the cluster MACSJ1424, which hosts both radio and submm point
sources. We examine the morphology of the tSZ signal and compare it to other
datasets. The NIKA data are combined with Herschel satellite data to study the
SED of the submm point source contaminants. We then perform a joint
reconstruction of the intracluster medium (ICM) electronic pressure and density
by combining NIKA, Planck, XMM-Newton, and Chandra data, focusing on the impact
of the radio and submm sources on the reconstructed pressure profile. We find
that large-scale pressure distribution is unaffected by the point sources
because of the resolved nature of the NIKA observations. The reconstructed
pressure in the inner region is slightly higher when the contribution of point
sources are removed. We show that it is not possible to set strong constraints
on the central pressure distribution without accurately removing these
contaminants. The comparison with X-ray only data shows good agreement for the
pressure, temperature, and entropy profiles, which all indicate that MACSJ1424
is a dynamically relaxed cool core system. The present observations illustrate
the possibility of measuring these quantities with a relatively small
integration time, even at high redshift and without X-ray spectroscopy.Comment: 15 pages, 17 figures, submitted to A&
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