Introducing Polyautoimmunity: Secondary Autoimmune Diseases No Longer Exist

Similar pathophysiological mechanisms within autoimmune diseases have stimulated searches for common genetic roots. Polyautoimmunity is defined as the presence of more than one autoimmune disease in a single patient. When three or more autoimmune diseases coexist, this condition is called multiple autoimmune syndrome (MAS). We analyzed the presence of polyautoimmunity in 1,083 patients belonging to four autoimmune disease cohorts. Polyautoimmunity was observed in 373 patients (34.4%). Autoimmune thyroid disease (AITD) and Sjögren's syndrome (SS) were the most frequent diseases encountered. Factors significantly associated with polyautoimmunity were female gender and familial autoimmunity. Through a systematic literature review, an updated search was done for all MAS cases (January 2006–September 2011). There were 142 articles retrieved corresponding to 226 cases. Next, we performed a clustering analysis in which AITD followed by systemic lupus erythematosus and SS were the most hierarchical diseases encountered. Our results indicate that coexistence of autoimmune diseases is not uncommon and follows a grouping pattern. Polyautoimmunity is the term proposed for this association of disorders, which encompasses the concept of a common origin for these diseases

Introducing polyautoimmunity: secondary autoimmune diseases no longer exist

Similar pathophysiological mechanisms within autoimmune diseases have stimulated searches for common genetic roots. Polyautoimmunity is defined as the presence of more than one autoimmune disease in a single patient. When three or more autoimmune diseases coexist, this condition is called multiple autoimmune syndrome (MAS). We analyzed the presence of polyautoimmunity in 1,083 patients belonging to four autoimmune disease cohorts. Polyautoimmunity was observed in 373 patients (34.4%). Autoimmune thyroid disease (AITD) and Sjögren's syndrome (SS) were the most frequent diseases encountered. Factors significantly associated with polyautoimmunity were female gender and familial autoimmunity. Through a systematic literature review, an updated search was done for all MAS cases (January 2006–September 2011). There were 142 articles retrieved corresponding to 226 cases. Next, we performed a clustering analysis in which AITD followed by systemic lupus erythematosus and SS were the most hierarchical diseases encountered. Our results indicate that coexistence of autoimmune diseases is not uncommon and follows a grouping pattern. Polyautoimmunity is the term proposed for this association of disorders, which encompasses the concept of a common origin for these diseases

Physicochemical Characterization And Antioxidant Capacity Of Pitanga Fruits (eugenia Uniflora L.) [caracterização Fisico-química E Capacidade Antioxidante De Pitangas (eugenia Uniflora L.)]

This study was carried out to obtain more information about the physicochemical properties, composition, and antioxidant activity of pitanga fruits (Eugenia uniflora L.), particularly fruits from the State of Rio Grande do Sul, Brazil. Pitanga with different flesh colors (purple, red, and orange) from tree selections cultivated at Embrapa Clima Temperado (RS-Brazil) were analyzed. Only slight differences were observed in the quality parameters and in the proximate and fatty acid compositions among the fruits studied. The extracts from purple-fleshed pitanga had the highest total phenolic and anthocyanin contents along with the highest antioxidant capacity. The antioxidant capacity (DPPH and FRAP assays) of methanolic pitanga extracts was highly correlated with the total phenolic content, but in ethanolic extracts, the anthocyanin content was correlated only with the FRAP antioxidant capacity. Orange fleshed pitanga had higher β-cryptoxanthin and β-carotene levels than those of the red fruit, which had higher lycopene content. The results indicate that the purple-fleshed pitanga, cultivated in Rio Grande do Sul, is a rich source of phenolic compounds and has high antioxidant capacity. The red and orange-fleshed pitanga, on the other hand, are rich sources of carotenoids.311147154Abidille, M.D.H., Antioxidant activity of the extracts from Dillenia indica fruits (2005) Food Chemistry, 90 (4), pp. 891-896Adebajo, A.C., Oloki, K.J., Aladesanmi, A., Antimicrobial activity of the leaf extract of Eugenia uniflora (1989) Journal of Phytotherapy Resource, 3 (6), pp. 258-259Aherne, S.A., O'Brien, N.M., Dietary flavonols: Chemistry, food content, and metabolism (2002) Nutrition, 18 (1), pp. 75-81(1995) Official methods of analysis of the Association of the Official Analytical Chemists, , ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS-AOAC, 16th ed. Arlington, Virginia: AOACAzevedo-Meleiro, C.H., Rodriguez-Amaya, D.B., Confirmation of the identity of the carotenoids of tropical fruits by HPLC-DAD and HPLC-MS (2004) Journal of Food Composition and Analysis, 17 (3-4), pp. 385-396Bagetti, M., Antioxidant capacity and composition of pitanga seeds (2009) Ciência Rural, 39 (8), pp. 204-2510Beekwilder, J., Antioxidant in raspberry: On-line analysis links antioxidant activity to a diversity of individual metabolites (2005) Journal of Agricultural and Food Chemistry, 53 (9), pp. 3313-3320Benzie, F.F.I., Strain, J.J., The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay (1996) Analytical Biochemistry, 239 (1), pp. 70-76Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Journal of Biochemistry and Physiology, 37 (8), pp. 911-917Block, G., Patterson, B., Subar, A., Fruits, vegetables and cancer prevention: A review of the epidemiological evidence (1992) Nutrition and Cancer, 18 (1), pp. 1-29Bors, W., Flavonoids as antioxidants: Determination of radical scavenging efficiencies (1990) Methods in Enzymology, 186, pp. 343-355Brand-Williams, W., Cuvelier, M.E., Berset, C., Use of a free radical method to evaluated antioxidant activity (1995) Lebensmittel-Wissenschaft und-Technologie, 28 (1), pp. 25-30Regulamento técnico geral para fixação dos padrões de identidade e qualidade para polpa de fruta (2000) Diário Oficial da República Federativa do Brasil, p. 54. , http://extranet.agricultura.gov.br/sislegisconsulta/consultarLegislacao. do?operacao=visualizar&id=7777, BRASIL. Instrução Normativa, no 1, de 7 de janeiro de 2000, Brasília, DF, 10 jan, Seção 1, Disponível em:, Acesso em: 18 dez. 2008Cavalcante, M.L., Rodriguez-Amaya, D.B., Carotenoid composition of the tropical fruits Eugenia uniflora and Malpighia glabra (1992) Food Science and Human Nutrition, pp. 643-650. , In: CHARALAMBOUS, G. (Ed.), Amsterdam: Elsevier Science PublishersClinton, S.K., Lycopene: Chemistry, biology, and implications for human health and disease (1998) Nutrition Reviews, 56 (2), pp. 35-51Consolini, A.E., Sarubbio, M., Pharmacological effects of Eugenia uniflora L. (Myrtaceae) aqueous extract on rat's heart (2002) Journal of Ethnopharmacology, 81 (1), pp. 57-63di Mascio, P., Kaiser, S., Sies, H., Lycopene as the most efficient biological carotenoid singlet oxygen quencher (1989) Archives of Biochemistry and Biophysics, 274 (2), pp. 532-538Dillard, C.J., German, J.B., Phytochemicals: Neutraceuticals and human health (2000) Journal of the Science of Food and Agriculture, 80 (12), pp. 1744-1756Diplock, A.T., Functional food sciences and defense against reactive oxidative species (1998) British Journal of Nutrition, 80 (1), pp. 77-112Escarpa, A., Gonzalez, M.C., Approach to the content of total extractable phenolic compounds from different food samples by comparison of chromatographic and spectrophotometric methods (2001) Analytica Chimica Acta, 427 (1), pp. 119-127Gemtchüjnicov, I.D., (1976) Manual de taxonomia vegetal: Plantas de interesse econômico, agrícola, ornamentais e medicinais, p. 368. , São Paulo: CeresGenovese, M.I., Bioactive compounds and antioxidant capacity of exotic fruits commercial frozen pulps from Brazil (2008) Food Science and Technology International, 4 (3), pp. 207-214Hartman, L., Lago, B.C., A rapid preparation of fatty methyl esters from lipids (1973) Laboratory Practice, 22 (6), pp. 475-477Hassimotto, N.M.A., Genovese, M.I., Lajolo, F.M., Antioxidant activity of dietary fruits, vegetables, and commercial frozen fruit pulps (2005) Journal of Agricultural and Food Chemistry, 53 (8), pp. 2928-2935Kaur, C., Kapoor, H., Antioxidants in fruits and vegetables-the millennium's health (2001) International Journal of Food Science and Technology, 36 (7), pp. 703-725Kimura, M., Rodriguez-Amaya, D.B., Yokoyama, S.M., Cultivar differences and geographic effects on the carotenoid composition and vitamin A value of papaya (1991) Lebensmittel-Wissenschaft und-Technologie, 24 (5), pp. 415-418Krinsky, N.I., Johnson, E.J., Carotenoid actions and their relation to health and disease (2005) Molecular Aspects of Medicine, 26 (6), pp. 459-516Kris-Etherton, P.M., Bioactive compounds in foods: Their role in the prevention of cardiovascular disease and cancer (2002) American Journal of Medicine, 113 (9), pp. 71-88Kuskoski, M.E., Frutas tropicais silvestres e polpas de frutas congeladas: Atividade antioxidante, polifenóis e antocianinas (2006) Ciência Rural, 36 (4), pp. 1283-1287Lees, D.H., Francis, F.J., Standardization of pigment analyses in cranberries (1972) Hortscience, 7 (1), pp. 83-84Lima, V.L.A.G., Mélo, E.A., Lima, D.E.S., Fenólicos e carotenóides totais em pitanga (2002) Scientia Agricola, 59 (3), pp. 447-450Niizu, P.Y., Rodriguez-Amaya, D.B., A melancia como fonte de licopeno (2003) Revista do Instituto Adolfo Lutz, 62 (3), pp. 195-199Oliveira, A.L., Volatile compounds from pitanga fruit (Eugenia uniflora L.) (2006) Food Chemistry, 99 (1), pp. 1-5Padula, M., Rodriguez-Amaya, D.B., Characterization of the carotenoids and assessment of the vitamin A value of Brazilian guavas (Psidium guajava L.) (1986) Food Chemistry, 20 (1), pp. 11-19Pellegrini, N., Evaluation of antioxidant capacity of some fruit and vegetable foods: Efficiency of extraction of a sequence of solvents (2007) Journal of the Science of Food and Agriculture, 87 (1), pp. 103-111Pietta, P.G., Flavonoids as antioxidants (2000) Journal of Natural Products, 63 (7), pp. 1035-1042Pinto, M.S., Lajolo, F.M., Genovese, M.I., Bioactive compounds and quantification of total ellagic acid in strawberries (Fragaria × ananassa Duch.) (2007) Food Chemistry, 107 (4), pp. 1629-1635Porcu, O.M., Rodriguez-Amaya, D.B., Variation in the carotenoid composition of the lycopene-rich Brazilian fruit Eugenia uniflora L (2008) Plant Foods for Human Nutrition, 63 (4), pp. 195-199Prior, R.L., Cao, G., Antioxidant phytochemicals in fruits and vegetables: Diet and health implications (2000) Horticulture Science, 35 (4), pp. 588-592Rahman, I., Adcock, I.M., Oxidative stress and redox regulation of lung inflammation in COPD (2006) European Respiratory Journal, 28 (1), pp. 219-242Reynerston, K.A., Quantitative analysis of antiradical phenolic constituents from fourteen edible Myrtaceae fruits (2008) Food Chemistry, 109 (4), pp. 883-890Robards, K., Antolovich, M., Analytical chemistry of fruit bioflavonoids (1997) Analyst, 122, pp. 11R-34RRodriguez-Amaya, D.B., (1999) A guide to carotenoid analysis in foods, , Washington, D.C.: ILSI PressSalgado, S.M., Guerra, N.B., Melo Filho, A.B., Frozen fruit pulps: Effects of the processing on dietary fiber contents (1999) Brazilian Journal of Nutrition, 12 (3), pp. 303-308Scalzo, J., Plant genotype affects total antioxidant capacity and phenolic contents in fruit (2005) Nutrition, 21 (2), pp. 207-213Singleton, V.L., Rossi Jr., J.A., Colorimetry of total phenolic with phosphomolybdic-phosphotungstic acid reagents (1965) American Journal of Enology and Viticulture, 16 (3), pp. 144-158Stahl, W., Sies, H., Bioactivity and protective effects of natural carotenoids (2005) Biochimica et Biophysica Acta, 1740 (2), pp. 101-107Tapiero, H., Townsend, D.M., Tew, K.D., The role of carotenoids in the prevention of human pathologies (2004) Biomedicine and Pharmacotherapy, 58 (2), pp. 100-110(2006) Tabela brasileira de composição de alimentos-TACO, p. 113. , UNIVERSIDADE DE CAMPINAS-UNICAMP, 2. ed. Version II. Campinas: NEPA/UNICAMPVison, J.A., Phenol antioxidant quantity and quality in foods: Vegetables (1998) Journal of Agricultural and Food Chemistry, 46 (9), pp. 4113-4117Weyerstahl, P., Volatile constituents of Eugenia uniflora leaf oil (1988) Planta Médica, 54 (6), pp. 546-54

Classification tools for carotenoid content estimation in Manihot esculenta via metabolomics and machine learning

Cassava genotypes (Manihot esculenta Crantz) with high pro-vitamin A activity have been identified as a strategy to reduce the prevalence of deficiency of this vitamin. The color variability of cassava roots, which can vary from white to red, is related to the presence of several carotenoid pigments. The present study has shown how CIELAB color measurement on cassava roots tissue can be used as a non-destructive and very fast technique to quantify the levels of carotenoids in cassava root samples, avoiding the use of more expensive analytical techniques for compound quantification, such as UV-visible spectrophotometry and the HPLC. For this, we used machine learning techniques, associating the colorimetric data (CIELAB) with the data obtained by UV-vis and HPLC, to obtain models of prediction of carotenoids for this type of biomass. Best values of R2 (above 90%) were observed for the predictive variable TCC determined by UV-vis spectrophotometry. When we tested the machine learning models using the CIELAB values as inputs, for the total carotenoids contents quantified by HPLC, the Partial Least Squares (PLS), Support Vector Machines, and Elastic Net models presented the best values of R2 (above 40%) and Root-Mean-Square Error (RMSE). For the carotenoid quantification by UV-vis spectrophotometry, R2 (around 60%) and RMSE values (around 6.5) are more satisfactory. Ridge regression and Elastic Network showed the best results. It can be concluded that the use colorimetric technique (CIELAB) associated with UV-vis/HPLC and statistical techniques of prognostic analysis through machine learning can predict the content of total carotenoids in these samples, with good precision and accuracy.CAPES -Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(407323/2013-9)info:eu-repo/semantics/publishedVersio

Assaying Total Carotenoids in Flours of Corn and Sweetpotato by Laser Photoacoustic Spectroscopy

This study describes the application of the laser photoacoustic spectroscopy (PAS) for quantification of total carotenoids (TC) in corn flours and sweetpotato flours. Overall, thirty-three different corn flours and nine sweetpotato flours were investigated. All PAS measurements were performed at room temperature using 488-nm argon laser radiation for excitation and mechanical modulation of 9 and 30 Hz. The measurements were repeated within a run and within several days or months. The UV–Vis spectrophotometry was used as the reference method. The concentration range that allows for the reliable analysis of TC spans a region from 1 to 40 mg kg−1 for corn flours and from 9 to 40 mg kg−1 for sweetpotato flours. In the case of sweetpotato flours, the quantification may extend even to 240 mg kg−1 TC. The estimated detection limit values for TC in corn and sweetpotato flours were 0.1 and 0.3 mg kg−1, respectively. The computed repeatability (n = 3–12) and intermediate precision (n = 6–28) RSD values at 9 and 30 Hz are comparable: 0.1–17.1% and 5.3–14.7% for corn flours as compared with 1.4–9.1% and 4.2–23.0% for sweetpotato flours. Our results show that PAS can be successfully used as a new analytical tool to simply and rapidly screen the flours for their nutritional potential based on the total carotenoid concentration

Prospective space model for San Francisco de Sales by 2036

13 páginas : mapasAnte la constante influencia espacial que ejerce la ciudad de Bogotá, capital de Colombia, en los municipios periféricos dentro del departamento de Cundinamarca, se evidencia, principalmente, por factores como su proximidad espacial a la capital y sus condiciones climáticas atractivas a los residentes citadinos para culminar su vejez, que las dinámicas del sistema urbano-rural tradicional están cambiando en el municipio de San Francisco de Sales. Por su localización estratégica provincial y al ser territorio de potencialidades sobre todo a nivel ambiental y para la despensa de alimentos, en este estudio se plantea un modelo prospectivo de ordenamiento territorial al año 2036 con el fin de contrarrestar los efectos perjudiciales del fenómeno desde la perspectiva de la nueva ruralidad.Given the constant spatial influence exerted by the city of Bogotá, capital of Colombia, in the peripheral municipalities within the department of Cundinamarca, it is evidenced, mainly, by factors such as its spatial proximity to the capital and its attractive climatic conditions for city residents to culminate its old age, that the dynamics of the traditional urban-rural system are changing in the municipality of San Francisco de Sales. Due to its provincial strategic location and being a territory of potentialities, especially at the environmental level and for the food pantry, this study proposes a prospective model of territorial planning to the year 2036 in order to counteract the harmful effects of the phenomenon from the perspective of the new rurality

Diversity for chemical composition in a collection of different varietal types of tree tomato (Solanum betaceum Cav.), an Andean exotic fruit

[EN] We evaluated 23 tree tomato (Solanum betaceum) accessions from five cultivar groups and one wild relative (Solanum cajanumense) for 26 composition traits. For all traits we found highly significant differences (P < 0.001) among the materials studied. The high diversity found within S. betaceum for composition traits was matched by a high diversity within each of the cultivar groups. We found that sucrose and citric acid were the most important soluble sugar and organic acid, respectively, in tree tomato. Fruit in the anthocyanin pigmented (purple) group had a carotenoid content similar to that in the yellow-orange cultivar groups. Total phenolic content was significantly correlated (r = 0.8607) with antioxidant activity. Analyses of mineral content showed that tree tomato is a good source of K, Mg, and Cu. Multivariate principal components analysis (PCA) confirmed that an important diversity exists within each cultivar group. The results we have obtained indicate that the high diversity found within the tree tomato could be exploited for selection and breeding for developing the tree tomato as a commercial crop. (C) 2014 Elsevier Ltd. All rights reserved.This work was partially financed by the Ecuadorian Secretaria Nacional de Educacion Superior, Ciencia, Tecnologia e Innovacion from Ecuador (SENESCYT).Acosta-Quezada, P.; Raigón Jiménez, MD.; Riofrío-Cuenca, T.; García Martínez, MD.; Plazas Ávila, MDLO.; Burneo, J.; Figueroa, JG.... (2015). Diversity for chemical composition in a collection of different varietal types of tree tomato (Solanum betaceum Cav.), an Andean exotic fruit. Food Chemistry. 169:327-335. doi:10.1016/j.foodchem.2014.07.152S32733516

Evaluación del impacto de la ingesta de arándano sobre los niveles de colesterol, glucosa y triglicéridos en ratas wistar alimentadas con dietas ricas en lípidos.

En México, una de las principales causas de muerte en personas de edad adulta son enfermedades coronarias. Según estudios realizados en los últimos años, el 26% de los mexicanos de la tercera edad presentan niveles altos de colesterol HDL (+200 mg/dl). Estos datos son muy similares a nivel mundial. Se estima que en México prevalecían índices elevados de desnutrición, aumentando las manifestaciones de mala nutrición por exceso (ENSANUT, 2012). El arándano o “blueberry” es un frutal menor perteneciente al género Vaccinium, de la familia Ericaceae (Sudzuki, 1983). El fruto del arándano es una baya casi esférica, que dependiendo de la especie y cultivar, su tamaño puede variar entre 0,7 a 1,5 cm de diámetro, con un color azul claro hasta oscuro, conteniendo en su interior hasta 100 semillas pequeñas (Buzeta, 1997). Numerosas investigaciones relacionan al arándano con el aumento de colesterol HDL, lo que estaría asociado a una disminución en la probabilidad de sufrir afecciones cardíacas; esto debido a su gran poder antioxidante. El objetivo de este trabajo es la medición de los efectos del consumo de arándano macerado sobre la reducción y control de los niveles de colesterol (LDL, HDL), glucosa y triglicéridos en ratas Wistar en edad reproductiva