Seawi—a sea urchin piwi/argonaute family member is a component of MT-RNP complexes

This is the publisher's version, also available electronically from http://rnajournal.cshlp.org/content/11/5/646.The piwi/argonaute family of proteins is involved in key developmental processes such as stem cell maintenance and axis specification through molecular mechanisms that may involve RNA silencing. Here we report on the cloning and characterization of the sea urchin piwi/argonaute family member seawi. Seawi is a major component of microtubule-ribonucleoprotein (MT-RNP) complexes isolated from two different species of sea urchin, Strongylocentrotus purpuratus and Paracentrotus lividus. Seawi co-isolates with purified ribosomes, cosediments with 80S ribosomes in sucrose density gradients, and binds microtubules. Seawi possesses the RNA binding motif common to piwi family members and binds P. lividus bep4 mRNA, a transcript that co-isolates with MT-RNP complexes and whose translation product has been shown to play a role in patterning the animal–vegetal axis. Indirect immunofluorescence studies localized seawi to the cortex of unfertilized eggs within granule-like particles, the mitotic spindle during cell division, and the small micromeres where its levels were enriched during the early cleavage stage. Lastly, we discuss how seawi, as a piwi/argonaute family member, may play a fundamentally important role in sea urchin animal–vegetal axis formation and stem cell maintenance

Evidence of an oceanic impact and megatsunami sedimentation in Chryse Planitia, Mars

In 1976, NASA's Viking 1 Lander (V1L) was the first spacecraft to operate successfully on the Martian surface. The V1L landed near the terminus of an enormous catastrophic flood channel, Maja Valles. However, instead of the expected megaflood record, its cameras imaged a boulder-strewn surface of elusive origin. We identified a 110-km-diameter impact crater (Pohl) ~ 900 km northeast of the landing site, stratigraphically positioned (a) above catastrophic flood-eroded surfaces formed ~ 3.4 Ga during a period of northern plains oceanic inundation and (b) below the younger of two previously hypothesized megatsunami deposits. These stratigraphic relationships suggest that a marine impact likely formed the crater. Our simulated impact-generated megatsunami run-ups closely match the mapped older megatsunami deposit's margins and predict fronts reaching the V1L site. The site's location along a highland-facing lobe aligned to erosional grooves supports a megatsunami origin. Our mapping also shows that Pohl's knobby rim regionally represents a broader history of megatsunami modification involving circum-oceanic glaciation and sedimentary extrusions extending beyond the recorded megatsunami emplacement in Chryse Planitia. Our findings allow that rocks and soil salts at the landing site are of marine origin, inviting the scientific reconsideration of information gathered from the first in-situ measurements on Mars

Martian outflow channels : How did their source aquifers form, and why did they drain so rapidly?

Catastrophic floods generated ~3.2 Ga by rapid groundwater evacuation scoured the Solar System's most voluminous channels, the southern circum-Chryse outflow channels. Based on Viking Orbiter data analysis, it was hypothesized that these outflows emanated from a global Hesperian cryosphere-confined aquifer that was infused by south polar meltwater infiltration into the planet's upper crust. In this model, the outflow channels formed along zones of superlithostatic pressure generated by pronounced elevation differences around the Highland-Lowland Dichotomy Boundary. However, the restricted geographic location of the channels indicates that these conditions were not uniform Boundary. Furthermore, some outflow channel sources are too high to have been fed by south polar basal melting. Using more recent mission data, we argue that during the Late Noachian fluvial and glacial sediments were deposited into a clastic wedge within a paleo-basin located in the southern circum-Chryse region, which was then completely submerged under a primordial northern plains ocean. Subsequent Late Hesperian outflow channels were sourced from within these geologic materials and formed by gigantic groundwater outbursts driven by an elevated hydraulic head from the Valles Marineris region. Thus, our findings link the formation of the southern circum-Chryse outflow channels to ancient marine, glacial, and fluvial erosion and sedimentation

Bounds for Lepton Flavor Violation and the Pseudoscalar Higgs in the General Two Higgs Doublet Model using g−2g-2 muon factor

Current experimental data from the $g-2$ muon factor, seems to show the necessity of physics beyond the Standard Model (SM), since the difference between SM and experimental predictions is 2.6$\sigma$. In the framework of the General Two Higgs Doublet Model (2HDM), we calculate the muon anomalous magnetic moment to get lower and upper bounds for the Flavour Changing (FC) Yukawa couplings in the leptonic sector. We also obtain lower bounds for the mass of the pseudoscalar Higgs ($m_{A^0}$) as a function of the parameters of the model.Comment: 12 pages, RevTex4, 5 figures. Improved presentation, updated experimental data, amplified analysis, new figures added. Subbmited to Phys. Rev.

Tsunami waves extensively resurfaced the shorelines of an early Martian ocean

It has been proposed that ~3.4 billion years ago an ocean fed by enormous catastrophic floods covered most of the Martian northern lowlands. However, a persistent problem with this hypothesis is the lack of definitive paleoshoreline features. Here, based on geomorphic and thermal image mapping in the circum-Chryse and northwestern Arabia Terra regions of the northern plains, in combination with numerical analyses, we show evidence for two enormous tsunami events possibly triggered by bolide impacts, resulting in craters ~30 km in diameter and occurring perhaps a few million years apart. The tsunamis produced widespread littoral landforms, including run-up water- ice-rich and bouldery lobes, which extended tens to hundreds of kilometers over gently sloping plains and boundary cratered highlands, as well as backwash channels where wave retreat occurred on highland-boundary surfaces. The ice-rich lobes formed in association with the younger tsunami, showing that their emplacement took place following a transition into a colder global climatic regime that occurred after the older tsunami event. We conclude that, on early Mars, tsunamis played a major role in generating and resurfacing coastal terrains

PHEMTO: the polarimetric high energy modular telescope observatory

Based upon dual focusing techniques, the Polarimetric High-Energy Modular Telescope Observatory (PHEMTO) is designed to have performance several orders of magnitude better than the present hard X-ray instruments, in the 1–600 keV energy range. This, together with its angular resolution of around one arcsecond, and its sensitive polarimetry measurement capability, will give PHEMTO the improvements in scientific performance needed for a mission in the 2050 era in order to study AGN, galactic black holes, neutrons stars, and supernovae. In addition, its high performance will enable the study of the non-thermal processes in galaxy clusters with an unprecedented accuracy.Open access funding provided by Istituto Nazionale di Astrofisica within the CRUI-CARE Agreement

Lepton Flavor Violation in the Two Higgs Doublet Model type III

We consider the Two Higgs Doublet Model (2HDM) of type III which leads to Flavour Changing Neutral Currents (FCNC) at tree level in the leptonic sector. In the framework of this model we can have, in principle, two situations: the case (a) when both doublets acquire a vacuum expectation value different from zero and the case (b) when only one of them is not zero. In addition, we show that we can make two types of rotations for the flavor mixing matrices which generates four types of lagrangians, with the rotation of type I we recover the case (b) from the case (a) in the limit $\tan \beta \to \infty$, and with the rotation of type II we obtain the case (b) from (a) in the limit $\tan \beta \to 0.$Moreover, two of the four possible lagrangians correspond to the models of types I and II plus Flavor Changing (FC) interactions. The analitical expressions of the partial lepton number violating widths $\Gamma (\mu \to eee)$ and $\Gamma (\mu \to e\gamma)$are derived for the cases (a) and (b) and both types of rotations.$$In all cases these widths go asymptotically to zero in the decoupling limit for all Higgses. We present from our analysis upper bounds for the flavour changing transition $\mu \to e,$ and we show that such bounds are sensitive to the VEV structure and the type of rotation utilized.Comment: 7 pages RevTeX4, 4 figures postscript, new section added and some new reference