Biorremediación en suelos contaminados con hidrocarburos

Se estudia la bioremediación de suelos contaminados con el interés de disminuir el efecto contaminante de hidrocarburos a niveles no tóxicos. Los ensayos a escala laboratorio, desarrollados en condiciones de temperatura, aireación y humedad controlada permiten evaluar alternativas de utilización de nutrientes, inoculación de bacterias y/o aprovechamiento de la microflora natural del suelo. Los resultados muestran la activación de la microflora natural por la utilización de nutrientes en proporción adecuada, obteniéndose remociones de hidrocaburo de 70 % en 55 días de proceso. Asimismo, se analiza la efectividad del proceso de bioremediación cuando se utiliza suelo recuperado. Así, la utilización de suelo virgen puede limitarse al mínimo evitando desmontes innecesarios para su provisión.It was studied contaminated soils bioremediation with the interest to decrease the hydrocarbon contaminant effect to no toxic levels. The laboratory assays, developed at temperature, aeration, and moisture controlled conditions let alternative nutrients, commercial microbes addition, and/or natural soil micro flora profit evaluation. The results show the natural micro flora activation because of the adequate proportions nutrients utilization. It was obtained 70 % petroleum hydrocarbon removal in 55 process days. Also, It can limit the native soils utilization and not allowing cutting trees when it is not necessary.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Biorremediación en suelos contaminados con hidrocarburos

Se estudia la bioremediación de suelos contaminados con el interés de disminuir el efecto contaminante de hidrocarburos a niveles no tóxicos. Los ensayos a escala laboratorio, desarrollados en condiciones de temperatura, aireación y humedad controlada permiten evaluar alternativas de utilización de nutrientes, inoculación de bacterias y/o aprovechamiento de la microflora natural del suelo. Los resultados muestran la activación de la microflora natural por la utilización de nutrientes en proporción adecuada, obteniéndose remociones de hidrocaburo de 70 % en 55 días de proceso. Asimismo, se analiza la efectividad del proceso de bioremediación cuando se utiliza suelo recuperado. Así, la utilización de suelo virgen puede limitarse al mínimo evitando desmontes innecesarios para su provisión.It was studied contaminated soils bioremediation with the interest to decrease the hydrocarbon contaminant effect to no toxic levels. The laboratory assays, developed at temperature, aeration, and moisture controlled conditions let alternative nutrients, commercial microbes addition, and/or natural soil micro flora profit evaluation. The results show the natural micro flora activation because of the adequate proportions nutrients utilization. It was obtained 70 % petroleum hydrocarbon removal in 55 process days. Also, It can limit the native soils utilization and not allowing cutting trees when it is not necessary.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Biorremediación en suelos contaminados con hidrocarburos

Se estudia la bioremediación de suelos contaminados con el interés de disminuir el efecto contaminante de hidrocarburos a niveles no tóxicos. Los ensayos a escala laboratorio, desarrollados en condiciones de temperatura, aireación y humedad controlada permiten evaluar alternativas de utilización de nutrientes, inoculación de bacterias y/o aprovechamiento de la microflora natural del suelo. Los resultados muestran la activación de la microflora natural por la utilización de nutrientes en proporción adecuada, obteniéndose remociones de hidrocaburo de 70 % en 55 días de proceso. Asimismo, se analiza la efectividad del proceso de bioremediación cuando se utiliza suelo recuperado. Así, la utilización de suelo virgen puede limitarse al mínimo evitando desmontes innecesarios para su provisión.It was studied contaminated soils bioremediation with the interest to decrease the hydrocarbon contaminant effect to no toxic levels. The laboratory assays, developed at temperature, aeration, and moisture controlled conditions let alternative nutrients, commercial microbes addition, and/or natural soil micro flora profit evaluation. The results show the natural micro flora activation because of the adequate proportions nutrients utilization. It was obtained 70 % petroleum hydrocarbon removal in 55 process days. Also, It can limit the native soils utilization and not allowing cutting trees when it is not necessary.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

We would like to thank members of JCRM’s laboratory and GENYO’s support units for helping with animal handling, technical assistance, and further discussion. The results published here are in part based upon data generated by the TCGA Research Network (www.cancer.gov/tcga). This manuscript will be part of CPT PhD Thesis belonging to the Doctorate Program on Biomedicine, UGR, Spain.Extracellular matrix remodeling within the tumor microenvironment has been recognized as a relevant dynamic framework during tumor growth. However, research on proteases that trigger this remodeling keeps revealing a wide range of actions including both pro- and anti-tumorigenic. The extracellular protease ADAMTS1 exemplifies this dual role. In this work, we first confirmed a positive correlation of ADAMTS1 with endothelial-like phenotype of human melanoma cells together with the finding of associated signatures, including key genes such as endothelial CDH5. Using a CRISPR-Cas9 approach, we observed that the inhibition of ADAMTS1 in an aggressive uveal melanoma model compromised its endothelial-like properties, and more importantly, caused a robust blockade on the progression of tumor xenografts. Although vasculature emerged affected in ADAMTS1-deficient tumors, the most relevant action implied the downregulation of endothelial CDH5 in tumor cells, in association with stemness markers. Indeed, melanoma sphere assays also revealed a deficient commitment to form spheres in the absence of ADAMTS1, directly correlating with stemness markers and, remarkably, also with CDH5. Finally, taking advantage of advanced bioinformatics tools and available public data of uveal melanomas, we disclosed new prognosis factors, including endothelial elements and ADAMTS proteases. Our findings support the key role of ADAMTS proteases for uveal melanoma development since earlier stages, modulating the complex crosstalk between extracellular matrix and the induction of stemness and endothelial-like features. To our knowledge, this is the first report that supports the development of therapeutic targets on the extracellular matrix to overcome uveal melanoma.This research was supported by Ministerio de Economía y Competitividad and Instituto de Salud Carlos III from Spain, co-financed by FEDER (PI16/00345 to JCRM) and from Consejería de Salud de la Junta de Andalucía (OH-0028-2018, PE-0225-2018)

Gallic Acid: A Natural Phenolic Compound Exerting Antitumoral Activities in Colorectal Cancer via Interaction with G-Quadruplexes

Natural phenolic compounds have gained momentum for the prevention and treatment of cancer, but their antitumoral mechanism of action is not yet well understood. In the present study, we screened the antitumoral potential of several phenolic compounds in a cellular model of colorectal cancer (CRC).We selected gallic acid (GA) as a candidate in terms of potency and selectivity and extensively evaluated its biological activity. We report on the role of GA as a ligand of DNA G-quadruplexes (G4s), explaining several of its antitumoral effects, including the transcriptional inhibition of ribosomal and CMYC genes. In addition, GA shared with other established G4 ligands some effects such as cell cycle arrest, nucleolar stress, and induction of DNA damage. We further confirmed the antitumoral and G4-stabilizing properties of GA using a xenograft model of CRC. Finally, we succinctly demonstrate that GA could be explored as a therapeutic agent in a patient cohort with CRC. Our work reveals that GA, a natural bioactive compound present in the diet, affects gene expression by interaction with G4s both in vitro and in vivo and paves the way towards G4s targeting with phenolic compounds.Instituto de Salud Carlos IIIEuropean Commission PI21/00497 AC18/00008Next generation EU, Plan de Recuperacion Transformacion y Resiliencia, Agencia Estatal de Investigacion PLEC2021-008094Ministerio de Ciencia e Innovacion from Government of Spain PID2019-104416RB-I00 PID2020-120481RB-I00Ministerio de Universidades from Government of Spain FPU16/05822 FPU17/05413 FPU20/03952University of Almeria FPI-20110

Fertilization and connectivity in the Garrucha Canyon (SE-Spain) Implications for Marine Spatial Planning

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Mortality and other adverse outcomes in patients with type 2 diabetes mellitus admitted for COVID-19 in association with glucose-lowering drugs: a nationwide cohort study

Background: Limited evidence exists on the role of glucose-lowering drugs in patients with COVID-19. Our main objective was to examine the association between in-hospital death and each routine at-home glucose-lowering drug both individually and in combination with metformin in patients with type 2 diabetes mellitus admitted for COVID-19. We also evaluated their association with the composite outcome of the need for ICU admission, invasive and non-invasive mechanical ventilation, or in-hospital death as well as on the development of in-hospital complications and a long-time hospital stay. Methods: We selected all patients with type 2 diabetes mellitus in the Spanish Society of Internal Medicine’s registry of COVID-19 patients (SEMI-COVID-19 Registry). It is an ongoing, observational, multicenter, nationwide cohort of patients admitted for COVID-19 in Spain from March 1, 2020. Each glucose-lowering drug user was matched with a user of other glucose-lowering drugs in a 1:1 manner by propensity scores. In order to assess the adequacy of propensity score matching, we used the standardized mean difference found in patient characteristics after matching. There was considered to be a significant imbalance in the group if a standardized mean difference > 10% was found. To evaluate the association between treatment and study outcomes, both conditional logit and mixed effect logistic regressions were used when the sample size was ≥ 100. Results: A total of 2666 patients were found in the SEMI-COVID-19 Registry, 1297 on glucose-lowering drugs in monotherapy and 465 in combination with metformin. After propensity matching, 249 patients on metformin, 105 on dipeptidyl peptidase-4 inhibitors, 129 on insulin, 127 on metformin/dipeptidyl peptidase-4 inhibitors, 34 on metformin/sodium-glucose cotransporter 2 inhibitor, and 67 on metformin/insulin were selected. No at-home glucose-lowering drugs showed a significant association with in-hospital death; the composite outcome of the need of intensive care unit admission, mechanical ventilation, or in-hospital death; in-hospital complications; or long-time hospital stays. Conclusions: In patients with type 2 diabetes mellitus admitted for COVID-19, at-home glucose-lowering drugs showed no significant association with mortality and adverse outcomes. Given the close relationship between diabetes and COVID-19 and the limited evidence on the role of glucose-lowering drugs, prospective studies are needed

Clinical guide of the Spanish Society of Nephrology on the prevention and treatment of peritoneal infection in peritoneal dialysis

[Resumen] Las infecciones peritoneales siguen constituyendo una complicación muy relevante de la diálisis peritoneal, por su incidencia todavía elevada y por sus importantes consecuencias clínicas, en términos de mortalidad, fracaso de la técnica y costes para el sistema sanitario. Las prácticas de prevención y tratamiento de esta complicación muestran una notable heterogeneidad derivada, entre otros factores, de la complejidad del problema y de la escasez de evidencia clínica que permitan responder de manera clara a muchas de las dudas planteadas. El propósito de este documento es proporcionar una revisión completa y actualizada de los métodos de diagnóstico, prevención y tratamiento de estas infecciones. El documento se ha elaborado tomando como referencia de partida la guía más reciente de la Sociedad Internacional de Diálisis Peritoneal (2016). Mientras que para el capítulo diagnóstico se ha adoptado una estructura más narrativa, el análisis de las medidas de prevención y tratamiento ha seguido una metodología sistemática (Grading of Recommendations, Assessment, Development and Evaluation [GRADE]), que especifica el nivel de evidencia y la fuerza de las sugerencias y recomendaciones propuestas, y facilita actualizaciones futuras de la guía. La gran extensión y numerosas recomendaciones o sugerencias emanadas de la revisión ponen de manifiesto la complejidad y gran número de facetas a tener en cuenta para un adecuado abordaje de esta importante complicación de la diálisis peritoneal.[Abstract] Peritoneal infections still represent a most feared complication of chronic peritoneal dialysis, due to their high incidence and relevant clinical consequences, including direct mortality, technique failure and a significant burden for the health system. The practices for prevention and treatment of this complication show a remarkable heterogeneity emerging, among other factors, from the complexity of the problem and from a paucity of quality evidence which could permit to respond clearly to many of the raised questions. The purpose of this document is to provide a complete and updated review of the main methods of diagnosis, prevention and treatment of these infections. The document has been elaborated taking as a reference the most recent guidelines of the International Society of Peritoneal Dialysis (2016). The diagnostic considerations are presented in a narrative style while, for prevention and therapy, we have used a systematic methodology (Grading of Recommendations, Assessment, Development and Evaluation [GRADE]), which specifies the level of evidence and the strength of the proposed suggestions and recommendations and facilitates future updates of the document. The length of the document and the many suggestions and recommendations coming out of the review underline the large number and the complexity of the factors to be taken into consideration for an adequate approach to this complication of peritoneal dialysis

Impact of Biological Agents on Postsurgical Complications in Inflammatory Bowel Disease : A Multicentre Study of Geteccu

Background: The impact of biologics on the risk of postoperative complications (PC) in inflammatory bowel disease (IBD) is still an ongoing debate. This lack of evidence is more relevant for ustekinumab and vedolizumab. Aims: To evaluate the impact of biologics on the risk of PC. Methods: A retrospective study was performed in 37 centres. Patients treated with biologics within 12 weeks before surgery were considered "exposed". The impact of the exposure on the risk of 30-day PC and the risk of infections was assessed by logistic regression and propensity score-matched analysis. Results: A total of 1535 surgeries were performed on 1370 patients. Of them, 711 surgeries were conducted in the exposed cohort (584 anti-TNF, 58 vedolizumab and 69 ustekinumab). In the multivariate analysis, male gender (OR: 1.5; 95% CI: 1.2-2.0), urgent surgery (OR: 1.6; 95% CI: 1.2-2.2), laparotomy approach (OR: 1.5; 95% CI: 1.1-1.9) and severe anaemia (OR: 1.8; 95% CI: 1.3-2.6) had higher risk of PC, while academic hospitals had significantly lower risk. Exposure to biologics (either anti-TNF, vedolizumab or ustekinumab) did not increase the risk of PC (OR: 1.2; 95% CI: 0.97-1.58), although it could be a risk factor for postoperative infections (OR 1.5; 95% CI: 1.03-2.27). Conclusions: Preoperative administration of biologics does not seem to be a risk factor for overall PC, although it may be so for postoperative infections