Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using illumina sequencing

Premise of the study: Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon.\ud \ud Methods and Results: Nineteen microsatellite loci were identified from an Illumina paired-end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ~80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380.\ud \ud Conclusions: These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history

Data from: Development of microsatellite markers for buffalograss (Buchloë dactyloides; Poaceae), a drought-tolerant turfgrass alternative

Premise of the study: Buchloë dactyloides is an important component of Great Plains prairies and a popular drought-tolerant turfgrass alternative in North America. This species comprises an autopolyploid series, and microsatellite primers were developed in order to understand the distribution of genetic variation among cytotypes and across its large geographic range. Methods and Results: Fifteen microsatellite loci were designed and successfully amplified in six B. dactyloides populations. Within-population genetic diversity was comparatively high, consistent with B. dactyloides' life history. Allelic variation at 13 loci was consistent with cytotype established in chromosome-counted samples. Conclusions: This variable, interpretable set of loci allows for the determination of multi-locus genotype in B. dactyloides individuals of varying cytotype. Data such as these from a range-wide sample set can provide important insights for germplasm conservation and crop improvement in this ecologically and economically important species

Read pair sequence information for buffalograss primers

Paired end sequence data used to design the 15 SSR loci reported in Hadle et al

Development of 28 polymorphic microsatellite markers for the endemic Azorean spider Sancus acoreensis (Araneae, Tetragnathidae)

Copyright © Springer Science+Business Media Dordrecht 2013."We isolated and characterized a total of 28 microsatellite loci from Sancus acoreensis. Loci were screened in 26 individuals originating from seven (Flores, Faial, Pico, São Jorge, Terceira, São Miguel, and Santa Maria) out of the nine islands of the Azores. The number of alleles per locus ranged from 2 to 14, observed heterozygosity ranged from 0.040 to 0.708, and the probability of identity values ranged from 0.02 to 0.97. Sancus acoreensis is a Laurel forest specialist species, endemic to the Azores, and is facing a great extinction risk due to the severe fragmentation of its habitat. The newly developed microsatellite loci will aid in detecting signs of population bottlenecks and pinpoint the island populations that are facing the greatest risk of extinction. […]"

Microsatellite records for volume 8, issue 1

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