In vitro viability of genipap pollen grains in different culture media

O objetivo deste trabalho foi avaliar a viabilidade in vitro do grão de pólen de jenipapeiro (Genipa americana) à temperatura ambiente, em diferentes meios de cultura. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 3x11 (meios de cultura x tempos de incubação), com quatro repetições. O número de grãos de pólen germinados foi analisado em intervalos de 24 horas até 288 horas após incubação em temperatura ambiente. O meio com 100 mg ha-1 de H3BO3, 80 g L-1 de sacarose e 1,0 L-1 de ágar resulta em alta à média percentagem de germinação in vitro, sendo o mais adequado para estudos da viabilidade in vitro de grãos de pólen de jenipapeiro.The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Ansiedade durante avaliações escolares: relatos e reflexões de ação extensionista com estudantes do ensino médio técnico

This article describes the experience of an outreach university action carried out by means of conversation circles that aimed to develop the awareness of technical high school students about the need to recognize manifestations of anxiety and the search for help. Another aim was to disseminate guidelines that help reduce anxiety in the school context. The activity was based on the outreach project Prevention of Risks and Diseases to Population Health. The facilitators involved in the activity were high school students from the third year of the Technical Nursing course under the supervision of teachers. The conversation circles allowed the observation of students´ point of view about anxiety, in particular the factors that trigger anxiety, in addition to providing moments for discussing guidelines for managing anxiety reactions.O objetivo do presente trabalho foi descrever a experiência de uma ação extensionista, executada por meio de rodas de conversa, que tinha como proposta sensibilizar alunos do ensino médio técnico quanto à necessidade de reconhecer manifestações de ansiedade e a procura de ajuda, assim como disseminar orientações que auxiliam na redução da ansiedade no contexto escolar. Essa atividade teve como referência o projeto de extensão “Prevenção de Riscos e Agravos à Saúde da População: contribuições para a Prática Assistencial no Nível Técnico em Enfermagem”. O trabalho contou com facilitadores compostos por alunos do terceiro ano médio técnico em Enfermagem, sob supervisão docente. As rodas de conversa permitiram a observação do ponto de vista dos alunos sobre a ansiedade, em especial os fatores desencadeantes de reações ansiosas, assim como foram momentos de discutir orientações sobre o manejo das reações ansiosas

Núcleos de Ensino da Unesp: artigos 2013: volume 2: metodologias de ensino e a apropriação de conhecimento pelos alunos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Núcleos de Ensino da Unesp: artigos 2013: volume 2: metodologias de ensino e a apropriação de conhecimento pelos alunos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP