The Effective Lorentzian and Teleparallel Spacetimes Generated by a Free Electromagnetic Field

In this paper we show that a free electromagnetic field living in Minkowski spacetime generates an effective Weitzenbock or an effective Lorentzian spacetime whose properties aredetermined in details. These results are possible because we found using the Clifford bundle formalism the noticeable result that the energy-momentum densities of a free electromagnetic field are sources of the Hodge duals of exact 2-form fields which satisfy Maxwell like equations.Comment: A missing term in Eq.(14) has been inserted and some misprints correcte

The DNA damage response is developmentally regulated in the African trypanosome

Genomes are affected by a wide range of damage, which has resulted in the evolution of a number of widely conserved DNA repair pathways. Most of these repair reactions have been described in the African trypanosome Trypanosoma brucei, which is a genetically tractable eukaryotic microbe and important human and animal parasite, but little work has considered how the DNA damage response operates throughout the T. brucei life cycle. Using quantitative PCR we have assessed damage induction and repair in both the nuclear and mitochondrial genomes of the parasite. We show differing kinetics of repair for three forms of DNA damage, and dramatic differences in repair between replicative life cycle forms found in the testse fly midgut and the mammal. We find that mammal-infective T. brucei cells repair oxidative and crosslink-induced DNA damage more efficiently than tsetse-infective cells and, moreover, very distinct patterns of induction and repair of DNA alkylating damage in the two life cycle forms. We also reveal robust repair of DNA lesions in the highly unusual T. brucei mitochondrial genome (the kinetoplast). By examining mutants we show that nuclear alkylation damage is repaired by the concerted action of two repair pathways, and that Rad51 acts in kinetoplast repair. Finally, we correlate repair with cell cycle arrest and cell growth, revealing that induced DNA damage has strikingly differing effects on the two life cycle stages, with distinct timing of alkylation-induced cell cycle arrest and higher levels of damage induced death in mammal-infective cells. Our data reveal that T. brucei regulates the DNA damage response during its life cycle, a capacity that may be shared by many microbial pathogens that exist in variant environments during growth and transmission

Composição corporal e perfil metabólico na deficiência de vitamina D sérica em adultos

sem informaçãoTo investigate the body composition and metabolic profile in individuals in terms of different concentrations of serum vitamin D, ranging from deficiency to sufficiency. A cross-sectional study of 106 adults of both genders, who were divided into three g304419430sem informaçãosem informaçãosem informaçã

Inactivation of capsaicin-sensitive nerves reduces pulmonary remodeling in guinea pigs with chronic allergic pulmonary inflammation

Pulmonary remodeling is an important feature of asthma physiopathology that can contribute to irreversible changes in lung function. Although neurokinins influence lung inflammation, their exact role in the extracellular matrix (ECM) remodeling remains to be determined. Our objective was to investigate whether inactivation of capsaicin-sensitive nerves modulates pulmonary ECM remodeling in animals with chronic lung inflammation. After 14 days of capsaicin (50 mg/kg, sc) or vehicle administration, male Hartley guinea pigs weighing 250-300 g were submitted to seven inhalations of increasing doses of ovalbumin (1, 2.5, and 5 mg/mL) or saline for 4 weeks. Seventy-two hours after the seventh inhalation, animals were anesthetized and mechanically ventilated and the lung mechanics and collagen and elastic fiber content in the airways, vessels and lung parenchyma were evaluated. Ovalbumin-exposed animals presented increasing collagen and elastic fiber content, respectively, in the airways (9.2 ± 0.9; 13.8 ± 1.2), vessels (19.8 ± 0.8; 13.4 ± 0.5) and lung parenchyma (9.2 ± 0.9; 13.8 ± 1.2) compared to control (P < 0.05). Capsaicin treatment reduced collagen and elastic fibers, respectively, in airways (1.7 ± 1.1; 7.9 ± 1.5), vessels (2.8 ± 1.1; 4.4 ± 1.1) and lung tissue (2.8 ± 1.1; 4.4 ± 1.1) of ovalbumin-exposed animals (P < 0.05). These findings were positively correlated with lung mechanical responses to antigenic challenge (P < 0.05). In conclusion, inactivation of capsaicin-sensitive nerve fibers reduces pulmonary remodeling, particularly collagen and elastic fibers, which contributes to the attenuation of pulmonary functional parameters

Collagen XVIII/endostatin expression in experimental endotoxemic acute renal failure

Acute renal failure (ARF) is a frequent complication of Gram-negative sepsis, with a high risk of mortality. Lipopolysaccharide (LPS)-induced ARF is associated with hemodynamic changes that are strongly influenced by the overproduction of nitric oxide (NO) through the cytokine-mediated up-regulation of inducible NO synthase. LPS-induced reductions in systemic vascular resistance paradoxically culminate in renal vasoconstriction. Collagen XVIII is an important component of the extracellular matrix expressed in basement membranes. Its degradation by matrix metalloproteases, cathepsins and elastases results in the formation of endostatin, claimed to have antiangiogenic activity and to be a prominent vasorelaxing agent. We evaluated the expression of endostatin/collagen XVIII in an endotoxemic ARF model. ARF was induced in C57BL/6 mice by intraperitoneal injection of LPS (10 mg/kg) followed by sacrifice 4 and 12 h later. Kidney tissue was the source of RNA and protein and the subject of histological analysis. As early as 4 h after LPS administration, blood urea, creatinine and NO levels were significantly increased compared to control. Endostatin/collagen XVIII mRNA levels were 0.71 times lower than sham-inoculated mice 4 h after LPS inoculation, returning to normal levels 12 h after LPS inoculation. Immunohistological examination revealed that acute injury caused by LPS leads to an increase of endostatin basement membrane staining in association with the decrease of CD31 endothelial basement membrane staining. These results indicate that in the early phase of endotoxemic ARF the endostatin levels were not regulated by gene expression, but by the metabolism of collagen XVIII

Assessing uncertainties in estimating surface energy fluxes from remote sensing over natural grasslands in Brazil

Evapotranspiration (ET) is one of the main fluxes in the global water cycle. As the Brazilian Pampa biome carries a rich biodiversity, accurate information on the ET dynamics is essential to support its proper monitoring and establish conservation strategies. In this context, we assessed an operational methodology based on the Simplified Surface Energy Balance Index (S-SEBI) model to estimate energy fluxes over the natural grasslands of the Pampa between 2014 and 2019. The S-SEBI is an ET model that requires a minimum of meteorological inputs and has demonstrated reasonable accuracy worldwide. Therefore, we investigated the model performance considering radiation data from both ERA5 reanalysis and Eddy Covariance measurements from a flux tower. Furthermore, comparisons from satellite-based estimates with in situ measurements were performed with and without energy balance closure (EBC). Results indicated that the meteorological inputs have low sensitivity on daily ET estimates from the S-SEBI model. In contrast, the instantaneous energy balance components are more affected. The strong seasonality impacts the evaporative fraction, which is more evident in late summer and autumn and may compromise the performance of the model in the biome. The effects in the daily ET are lower when in situ data without EBC are considered as ground truth. However, they are less correlated with the remote sensing-based estimates. These insights are useful to monitor water and energy fluxes from local to regional scale and provide the opportunity to capture ET trends over the natural grasslands of the Pampa

Effect of cattle trampling and farm machinery traffic on soil compaction of an Entic Haplustoll in a semiarid region of Argentina

Soil compaction has detrimental effects on the physical, mechanical and hydraulic properties of soils, and affects important soil processes and function, and crop productivity. This work was conducted to investigate soil compaction impacts in integrated arable croppinglivestock systems managed under conventional tillage (CT) and no-tillage (NT). The work examined the combined effects of cattle trampling and farm machinery traffic on: soil strength, soil deformation, and water infiltration into soil. The following treatments were applied to soil (Entic Haplustoll, 60% sand) managed under CT and NT: three traffic intensities (1, 5, 7 passes) performed with light (2WD, 53 kN) and heavy (4WD, 100.4 kN) tractors, and two stocking densities (400 and 700 kg ha-1 ), respectively. Controls were also used to represent the condition of the soil without any effect of livestock or field traffic. In both tillage systems, soil penetration resistance (strength) increased and water infiltration into soil decreased as traffic intensities or stocking rates applied increased. There was a significant traffic intensity × stocking rate interaction, which influenced the depth and extent of soil compaction at depth. Despite these results, stubble grazing during fallow should not be discouraged as this practice offers mixed farming systems several agronomic and financial benefits. If stubble was to be grazed, the system would need to be carefully managed: (1) avoid ‘random’ traffic using permanent or semipermanent traffic paths to minimise the field wheeled area, (2) vacate livestock from the field, or confine it to a sacrificial area, when the soil water content exceeds a critical level above which soil damage is likely, and (3) maintain more than 60%–70% ground cover. Tillage repair treatments can be targeted to those sacrificial or ‘hot-spots’ areas so that localised, as supposed to widespread, compaction problems are rectified before the next crop is established

Essential oil from Ageratum fastigiatum reduces expression of the pro-inflammatory cytokine tumor necrosis factor-alpha in peripheral blood leukocytes subjected to in vitro stimulation with phorbol myristate acetate

AbstractAgeratum fastigiatum (Gardner) R.M. King & H. Rob., a member of the Asteraceae family popularly known in Brazil as “matapasto”, is indicated in folk medicine as anti-inflammatory and analgesic. Despite its popular use, little is known about its potential effect on the parameters involved in an inflammatory response. The objective of this study was to characterize the chemical composition of the essential oil from A. fastigiatum and to evaluate the frequency of tumor necrosis factor alpha and interferon gamma producing cells in peripheral blood lymphocytes stimulated with phorbol myristate acetate in the presence of essential oil from A. fastigiatum. Non-toxic concentrations of essential oil from A. fastigiatum were evaluated in cultures of peripheral blood leucocytes using the trypan blue exclusion assay by flow cytometry. GC–MS analysis revealed that the prevalent compounds identified in the essential oil from A. fastigiatum sample were α-pinene, limonene, trans-caryophyllene, α-humulene, caryophyllene oxide, 1,2-humulene-epoxide, 1,6-humulanodien-3-ol, and α-cadinol. Results showed that exposure to essential oil from A. fastigiatum at concentrations of 0.5×10−2 and 1×10−2μl/ml caused no alterations in leukocyte viability as compared to the control group. Both concentrations lowered the percentage of tumor necrosis factor alpha (+)-lymphocytes and neutrophils. There were no changes in the percentage of lymphocytes positive for the interferon gamma cytokine. Our results suggest that part of the anti-inflammatory activity attributed to A. fastigiatum may be due to the effect of some of its components in decreasing the number of cells that produce the pro-inflammatory cytokine tumor necrosis factor alpha

Gravitational Energy of Kerr and Kerr Anti-de Sitter Space-times in the Teleparallel Geometry

In the context of the Hamiltonian formulation of the teleparallel equivalent of general relativity we compute the gravitational energy of Kerr and Kerr Anti-de Sitter (Kerr-AdS) space-times. The present calculation is carried out by means of an expression for the energy of the gravitational field that naturally arises from the integral form of the constraint equations of the formalism. In each case, the energy is exactly computed for finite and arbitrary spacelike two-spheres, without any restriction on the metric parameters. In particular, we evaluate the energy at the outer event horizon of the black holes.Comment: 11 pages, 1 figure, to appear in JHEP11(2003)00