The electron spectra in the synchrotron nebula of the supernova remnant G 29.7-0.3

EXOSAT results obtained with the imaging instrument (CMA) and the medium energy proportional counters (ME) are discussed. Assuming that the featureless power-law spectrum obtained in the 2 to 10 keV range is synchrotron radiation from relativistic electrons, one derives constraints on magnetic field strength and age of the nebula. The energy spectra of the electrons responsible for the emission in the radio and X-ray ranges are discussed. The great similarity of the physical properties of G 29.7-0.3 and of three synchrotron nebulae containing a compact object observed to pulse in X-rays makes G 29.7 - 0.3 a very promising candidate for further search for pulsed emission. Further observations at infrared wavelengths might reveal the break(s) in the emitted spectrum expected from the radio and X-ray power-law indices and give us more information on the production of the electron populations responsible for the emission of the nebula

Diffuse 0.5-1 keV X-Rays and Nuclear Gamma-Rays from Fast Particles in the Local Hot Bubble

We show that interactions of fast particles with the boundary shell of the local hot bubble could make an important contribution to the 0.5-1 keV diffuse X-ray background observed with ROSAT. The bulk of these nonthermal X-rays are due to line emission from fast O ions of energies around 1 MeV/nucleon. This is the typical energy per particle in the ejecta of the supernova which is thought to have energized the bubble. We find that there is sufficient total energy in the ejecta to produce X-rays of the required intensity, subject to the details of the evolution of the fast particle population since the supernova explosion (about 3 10$^5$ years ago based on the age of the Geminga pulsar). The unequivocal signature of lines from deexcitations in fast ions is their large width ($\delta E/E$~0.1 for O lines), which will clearly distinguishes them from X-ray lines produced in a hot plasma. If a small fraction of the total ejecta energy is converted into accelerated particle kinetic energy (>~30 MeV/nucleon), the gamma-ray line emission produced in the boundary shell of the local hot bubble could account for the recently reported COMPTEL observations of nuclear gamma-ray lines from a broad region towards the Galactic center.Comment: 13 pages, 4 figures, submitted to Ap

Laboratory studies on a spherically curved Bragg spectrometer for cosmic X-ray spectroscopy

A spherical array of twenty LiF 200 crystals was built to test the performances of a freestanding, self-focussing spherical crystal cosmic X-ray spectrometer. Measurements presently available show that the size of the image for a point source at infinite distance would be 3 mm (FWHM) along the focalisation axis and 2.1 mm (FWHM) along the dispersion axis. The mosaic spread on individual crystals is less than 0.1 degree. A slightly systematic deviation from the ideal bending (0.1 degree) is observed at the edges of most crystals and this appears to be the major limitation to spectrometer performance

Search for the Tunguska event in the Antarctic snow

The Tunguska explosion in 1908 is supposed to have been produced by the impact of a small celestial body. The absence of any identifiable crater together with the huge energy released by the event suggest that the impactor exploded in midair and that its material was widely spread over the Earth. The short term contribution of such exceptional events to the total accretion rate of extraterrestrial material by the Earth could be significant. Samples were chosen in a core electromechanically drilled in 1984 near South Pole Station. There, the low temperatures, preventing melting all year long, and the nearly regular snow fall rate provide good conditions for a reliable continuous record of any infalling material. In many samples Ir was below the detection limit of the instrumentation. The iridium infall averaged over 45 samples is given. In a few samples the iridium content is significantly higher than the average: the frequency and amplitude of such fluctuations can be explained by the presence on some filters of finite size cosmic particles. No significant systematic increase above the average level is observed in the part of the core corresponding to the Tunguska event. The two major results of this study are: (1) The presence of Tunguska explosion debris in the Antarctic snow is not confirmed; and (2) The estimate of the average iridium infall, is an order of magnitude lower than the Ganapathy's background but is close to the values measured in Antarctic snow and atmospheric samples by Takahashi et al. The results are also consistent with the flux of micrometeoroids deduced from optical and radar observations or derived from the study of Greenland cosmic dust collection but are lower than the flux at mid-latitude measured in paleocene-oligocene sediments from the central part of the Pacific Ocean

Butterfly distribution along altitudinal gradients: temporal changes over a short time period

Mountain ecosystems are particularly sensitive to changes in climate and land cover, but at the same time, they can offer important refuges for species on the opposite of the more altered lowlands. To explore the potential role of mountain ecosystems in butterfly conservation and to assess the vulnerability of the alpine species, we analyzed the short-term changes (2006-2008 vs. 2012-2013) of butterflies\u27 distribution along altitudinal gradients in the NW Italian Alps. We sampled butterfly communities once a month (62 sampling stations, 3 seasonal replicates per year, from June to August) by semi-quantitative sampling techniques. The monitored gradient ranges from the montane to the alpine belt (600-2700 m a.s.l.) within three protected areas: Gran Paradiso National Park (LTER, Sitecode: LTER_EU_IT_109), Orsiera Rocciavr? Natural Park and Veglia Devero Natural Park. We investigated butterflies\u27 temporal changes in accordance with a hierarchical approach to assess potential relationships between species and community level. As a first step, we characterized each species in terms of habitat requirements, elevational range and temperature preferences and we compared plot occupancy and altitudinal range changes between time periods (2006-2008 vs. 2012-2013). Secondly, we focused on community level, analyzing species richness and community composition temporal changes. The species level analysis highlighted a general increase in mean occupancy level and significant changes at both altitudinal boundaries. Looking at the ecological groups, we observed an increase of generalist and highly mobile species at the expense of the specialist and less mobile ones. For the community level, we noticed a significant increase in species richness, in the community temperature index and a tendency towards homogenization within communities. Besides the short time period considered, butterflies species distribution and communities changed considerably. In light of these results, it is fundamental to continue monitoring activities to understand if we are facing transient changes or first signals of an imminent trend

Tuning Local Hydration Enables a Deeper Understanding of Protein-Ligand Binding: The PP1-Src Kinase Case

Water plays a key role in biomolecular recognition and binding. Despite the development of several computational and experimental approaches, it is still challenging to comprehensively characterize water-mediated effects on the binding process. Here, we investigate how water affects the binding of Src kinase to one of its inhibitors, PP1. Src kinase is a target for treating several diseases, including cancer. We use biased molecular dynamics simulations, where the hydration of predetermined regions is tuned at will. This computational technique efficiently accelerates the SRC-PP1 binding simulation and allows us to identify several key and yet unexplored aspects of the solvent's role. This study provides a further perspective on the binding phenomenon, which may advance the current drug design approaches for the development of new kinase inhibitors

The Cretaceous-Palaeogene (K/P) boundary in the Aïn Settara section (Kalaat Senan, Central Tunisia): lithological, micropalaeontological and geochemical evidence

The Cretaceous-Palaeogene (K/P) boundary, until recently known as the "Cretaceous-Tertiary" or K/T boundary, is well exposed at Aïn Settara in the Kalaat Senan area (Central Tunisia), 50 km south of the El Kef section. Micropalaeontological and geochemical studies led to the identification of six main features tentatively named "events", which characterise the K/P boundary interval, and of which at least two (B and C) have global significance. The lowermost event A located at about 14 cm below the base of the Dark Boundary Clay is marked by a sudden increase in tiny bioturbations, by small nodules and a few macrofossils, a 50% drop in calcareous nannofossil abundance and an increase in Scytinascias (organic linings of foraminifera). It is thought to witness a slowdown in sedimentation. Event B is characterised by a burrowed surface, separating the ca 60-cm thick Dark Boundary Clay from the underlying Aïn Settara marls. It indicates an episode of nondeposition, just before a major change in lithology from marls to clays, corresponding to a major flooding. No substantial palaeontological changes have been recorded in relation to this event. Event C is characterised by maximum concentrations of Ir and Ni-rich spinels, which have been observed in platy nodules, similar to the level at El Kef (K/P boundary sensu ODIN, 1992). It coincides with a major extinction in planktonic foraminiferal species (71%) and a 60% drop in nannofossil abundance. The change in lithology (occurrence of small ripples and channel-like structures) recorded at event D, a few cm up-section, might be related to a locally recorded storm activity. Events E and F, which are situated higher up in the Dark Boundary Clay, are mainly determined by palaeontological changes (palynomorphs and nannofossils), probably resulting from small sea-level variations. The coincidence of the cosmic markers with the major biotic changes at event C pleads for the asteroid impact hypothesis. Their disjunction from the base of the Dark Boundary Clay shows that the change of lithology usually used to determine the K-P boundary is distinct from the major extinction (in the planktonic realm), classically referred to this boundary and linked to the presence of cosmic markers. These results argue the need for the revaluation of the K-P boundary GSSP at El Kef. It is suggested to redefine the K-P boundary at the level of coincidence of the major biotic changes and the cosmic markers

A Serendipitous Galaxy Cluster Survey with XMM: Expected Catalogue Properties and Scientific Applications

This paper describes a serendipitous galaxy cluster survey that we plan to conduct with the XMM X-ray satellite. We have modeled the expected properties of such a survey for three different cosmological models, using an extended Press-Schechter (Press & Schechter 1974) formalism, combined with a detailed characterization of the expected capabilities of the EPIC camera on board XMM. We estimate that, over the ten year design lifetime of XMM, the EPIC camera will image a total of ~800 square degrees in fields suitable for the serendipitous detection of clusters of galaxies. For the presently-favored low-density model with a cosmological constant, our simulations predict that this survey area would yield a catalogue of more than 8000 clusters, ranging from poor to very rich systems, with around 750 detections above z=1. A low-density open Universe yields similar numbers, though with a different redshift distribution, while a critical-density Universe gives considerably fewer clusters. This dependence of catalogue properties on cosmology means that the proposed survey will place strong constraints on the values of Omega-Matter and Omega-Lambda. The survey would also facilitate a variety of follow-up projects, including the quantification of evolution in the cluster X-ray luminosity-temperature relation, the study of high-redshift galaxies via gravitational lensing, follow-up observations of the Sunyaev-Zel'dovich effect and foreground analyses of cosmic microwave background maps.Comment: Accepted to ApJ. Minor changes, e.g. presentation of temperature errors as a figure (rather than as a table). Latex (20 pages, 6 figures, uses emulateapj.sty

Identification of Key Residues for pH Dependent Activation of Violaxanthin De-Epoxidase from Arabidopsis thaliana

Plants are often exposed to saturating light conditions, which can lead to oxidative stress. The carotenoid zeaxanthin, synthesized from violaxanthin by Violaxanthin De-Epoxidase (VDE) plays a major role in the protection from excess illumination. VDE activation is triggered by a pH reduction in the thylakoids lumen occurring under saturating light. In this work the mechanism of the VDE activation was investigated on a molecular level using multi conformer continuum electrostatic calculations, site directed mutagenesis and molecular dynamics. The pKa values of residues of the inactive VDE were determined to identify target residues that could be implicated in the activation. Five such target residues were investigated closer by site directed mutagenesis, whereas variants in four residues (D98, D117, H168 and D206) caused a reduction in enzymatic activity indicating a role in the activation of VDE while D86 mutants did not show any alteration. The analysis of the VDE sequence showed that the four putative activation residues are all conserved in plants but not in diatoms, explaining why VDE in these algae is already activated at higher pH. Molecular dynamics showed that the VDE structure was coherent at pH 7 with a low amount of water penetrating the hydrophobic barrel. Simulations carried out with the candidate residues locked into their protonated state showed instead an increased amount of water penetrating the barrel and the rupture of the H121–Y214 hydrogen bond at the end of the barrel, which is essential for VDE activation. These results suggest that VDE activation relies on a robust and redundant network, in which the four residues identified in this study play a major role