255 research outputs found
Clones, Thugs, \u27N (Eventual?) Harmony: Using the Federal Rules of Civil Procedure to Simulate a Statutory Defamation Defense and Make the World Safe From Copyright Bullies
Shoot First, Litigate Later: Declaratory Judgment Actions, Procedural Fencing, and Itchy Trigger Fingers
Deciphering the Roles of Multicomponent Recognition Signals by the AAA+ Unfoldase ClpX
ATP-dependent protein remodeling and unfolding enzymes are key participants in protein metabolism in all cells. How these often-destructive enzymes specifically recognize target protein complexes is poorly understood. Here, we use the well-studied AAA + unfoldase-substrate pair, Escherichia coli ClpX and MuA transposase, to address how these powerful enzymes recognize target protein complexes. We demonstrate that the final transposition product, which is a DNA-bound tetramer of MuA, is preferentially recognized over the monomeric apo-protein through its multivalent display of ClpX recognition tags. The important peptide tags include one at the C-terminus (“C-tag”) that binds the ClpX pore and a second one (enhancement or “E-tag”) that binds the ClpX N-terminal domain. We construct a chimeric protein to interrogate subunit-specific contributions of these tags. Efficient remodeling of MuA tetramers requires ClpX to contact a minimum of three tags (one C-tag and two or more E-tags), and that these tags are contributed by different subunits within the tetramer. The individual recognition peptides bind ClpX weakly (K[subscript D] > 70 μM) but impart a high-affinity interaction (K[subscript D] ~ 1.0 μM) when combined in the MuA tetramer. When the weak C-tag signal is replaced with a stronger recognition tag, the E-tags become unnecessary and ClpX's preference for the complex over MuA monomers is eliminated. Additionally, because the spatial orientation of the tags is predicted to change during the final step of transposition, this recognition strategy suggests how AAA + unfoldases specifically distinguish the completed “end-stage” form of a particular complex for the ideal biological outcome.National Institutes of Health (U.S.) (Grants GM-49224 and AI-16892)National Institutes of Health (U.S.) (NIH Pre-Doctoral Training Grant T32GM007287
Detection of the Power Spectrum of Cosmic Microwave Background Lensing by the Atacama Cosmology Telescope
We report the first detection of the gravitational lensing of the cosmic
microwave background through a measurement of the four-point correlation
function in the temperature maps made by the Atacama Cosmology Telescope. We
verify our detection by calculating the levels of potential contaminants and
performing a number of null tests. The resulting convergence power spectrum at
2-degree angular scales measures the amplitude of matter density fluctuations
on comoving length scales of around 100 Mpc at redshifts around 0.5 to 3. The
measured amplitude of the signal agrees with Lambda Cold Dark Matter cosmology
predictions. Since the amplitude of the convergence power spectrum scales as
the square of the amplitude of the density fluctuations, the 4-sigma detection
of the lensing signal measures the amplitude of density fluctuations to 12%.Comment: 4 pages, 4 figures, replaced title and author list with version
accepted by Physical Review Letters. Likelihood code can be downloaded from
http://bccp.lbl.gov/~sudeep/ACTLensLike.htm
The Atacama Cosmology Telescope: Temperature and Gravitational Lensing Power Spectrum Measurements from Three Seasons of Data
We present the temperature power spectra of the cosmic microwave background
(CMB) derived from the three seasons of data from the Atacama Cosmology
Telescope (ACT) at 148 GHz and 218 GHz, as well as the cross-frequency spectrum
between the two channels. We detect and correct for contamination due to the
Galactic cirrus in our equatorial maps. We present the results of a number of
tests for possible systematic error and conclude that any effects are not
significant compared to the statistical errors we quote. Where they overlap, we
cross-correlate the ACT and the South Pole Telescope (SPT) maps and show they
are consistent. The measurements of higher-order peaks in the CMB power
spectrum provide an additional test of the Lambda CDM cosmological model, and
help constrain extensions beyond the standard model. The small angular scale
power spectrum also provides constraining power on the Sunyaev-Zel'dovich
effects and extragalactic foregrounds. We also present a measurement of the CMB
gravitational lensing convergence power spectrum at 4.6-sigma detection
significance.Comment: 21 pages; 20 figures, Submitted to JCAP, some typos correcte
The Atacama Cosmology Telescope: Two-Season ACTPol Lensing Power Spectrum
We report a measurement of the power spectrum of cosmic microwave background
(CMB) lensing from two seasons of Atacama Cosmology Telescope Polarimeter
(ACTPol) CMB data. The CMB lensing power spectrum is extracted from both
temperature and polarization data using quadratic estimators. We obtain results
that are consistent with the expectation from the best-fit Planck LCDM model
over a range of multipoles L=80-2100, with an amplitude of lensing A_lens =
1.06 +/- 0.15 (stat.) +/- 0.06 (sys.) relative to Planck. Our measurement of
the CMB lensing power spectrum gives sigma_8 Omega_m^0.25 = 0.643 +/- 0.054;
including baryon acoustic oscillation scale data, we constrain the amplitude of
density fluctuations to be sigma_8 = 0.831 +/- 0.053. We also update
constraints on the neutrino mass sum. We verify our lensing measurement with a
number of null tests and systematic checks, finding no evidence of significant
systematic errors. This measurement relies on a small fraction of the ACTPol
data already taken; more precise lensing results can therefore be expected from
the full ACTPol dataset.Comment: 17 pages, 11 figures, to be submitted to Physical Review
Evidence for dark energy from the cosmic microwave background alone using the Atacama Cosmology Telescope lensing measurements
For the first time, measurements of the cosmic microwave background radiation
(CMB) alone favor cosmologies with dark energy over models without dark
energy at a 3.2-sigma level. We demonstrate this by combining the CMB lensing
deflection power spectrum from the Atacama Cosmology Telescope with temperature
and polarization power spectra from the Wilkinson Microwave Anisotropy Probe.
The lensing data break the geometric degeneracy of different cosmological
models with similar CMB temperature power spectra. Our CMB-only measurement of
the dark energy density confirms other measurements from
supernovae, galaxy clusters and baryon acoustic oscillations, and demonstrates
the power of CMB lensing as a new cosmological tool.Comment: 4 pages, 3 figures; replaced with version accepted by Physical Review
Letters, added sentence on models with non-standard primordial power spectr
The Atacama Cosmology Telescope: Sunyaev-Zel'dovich Selected Galaxy Clusters at 148 GHz from Three Seasons of Data
[Abridged] We present a catalog of 68 galaxy clusters, of which 19 are new
discoveries, detected via the Sunyaev-Zel'dovich effect (SZ) at 148 GHz in the
Atacama Cosmology Telescope (ACT) survey of 504 square degrees on the celestial
equator. A subsample of 48 clusters within the 270 square degree region
overlapping SDSS Stripe 82 is estimated to be 90% complete for M_500c > 4.5e14
Msun and 0.15 < z < 0.8. While matched filters are used to detect the clusters,
the sample is studied further through a "Profile Based Amplitude Analysis"
using a single filter at a fixed \theta_500 = 5.9' angular scale. This new
approach takes advantage of the "Universal Pressure Profile" (UPP) to fix the
relationship between the cluster characteristic size (R_500) and the integrated
Compton parameter (Y_500). The UPP scalings are found to be nearly identical to
an adiabatic model, while a model incorporating non-thermal pressure better
matches dynamical mass measurements and masses from the South Pole Telescope. A
high signal to noise ratio subsample of 15 ACT clusters is used to obtain
cosmological constraints. We first confirm that constraints from SZ data are
limited by uncertainty in the scaling relation parameters rather than sample
size or measurement uncertainty. We next add in seven clusters from the ACT
Southern survey, including their dynamical mass measurements based on galaxy
velocity dispersions. In combination with WMAP7 these data simultaneously
constrain the scaling relation and cosmological parameters, yielding \sigma_8 =
0.829 \pm 0.024 and \Omega_m = 0.292 \pm 0.025. The results include
marginalization over a 15% bias in dynamical mass relative to the true halo
mass. In an extension to LCDM that incorporates non-zero neutrino mass density,
we combine our data with WMAP7+BAO+Hubble constant measurements to constrain
\Sigma m_\nu < 0.29 eV (95% C. L.).Comment: 32 pages, 21 figures To appear in J. Cosmology and Astroparticle
Physic
Algae Bioreactor Using Submerged Enclosures with Semi-Permeable Membranes
Methods for producing hydrocarbons, including oil, by processing algae and/or other micro-organisms in an aquatic environment. Flexible bags (e.g., plastic) with CO.sub.2/O.sub.2 exchange membranes, suspended at a controllable depth in a first liquid (e.g., seawater), receive a second liquid (e.g., liquid effluent from a "dead zone") containing seeds for algae growth. The algae are cultivated and harvested in the bags, after most of the second liquid is removed by forward osmosis through liquid exchange membranes. The algae are removed and processed, and the bags are cleaned and reused
The Atacama Cosmology Telescope: Extragalactic Sources at 148 GHz in the 2008 Survey
We report on extragalactic sources detected in a 455 square-degree map of the
southern sky made with data at a frequency of 148 GHz from the Atacama
Cosmology Telescope 2008 observing season. We provide a catalog of 157 sources
with flux densities spanning two orders of magnitude: from 15 to 1500 mJy.
Comparison to other catalogs shows that 98% of the ACT detections correspond to
sources detected at lower radio frequencies. Three of the sources appear to be
associated with the brightest cluster galaxies of low redshift X-ray selected
galaxy clusters. Estimates of the radio to mm-wave spectral indices and
differential counts of the sources further bolster the hypothesis that they are
nearly all radio sources, and that their emission is not dominated by
re-emission from warm dust. In a bright (>50 mJy) 148 GHz-selected sample with
complete cross-identifications from the Australia Telescope 20 GHz survey, we
observe an average steepening of the spectra between 5, 20, and 148 GHz with
median spectral indices of , , and . When the
measured spectral indices are taken into account, the 148 GHz differential
source counts are consistent with previous measurements at 30 GHz in the
context of a source count model dominated by radio sources. Extrapolating with
an appropriately rescaled model for the radio source counts, the Poisson
contribution to the spatial power spectrum from synchrotron-dominated sources
with flux density less than 20 mJy is C^{\rm Sync} = (2.8 \pm 0.3) \times
10^{-6} \micro\kelvin^2.Comment: Accepted to Ap
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