Modern approaches to improving phase contrast electron microscopy

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a phase shift to the unscattered part of the electron beam. Many approaches have been investigated, including the ponderomotive interaction between light and electrons. We review the recent successes achieved with this method in high-resolution, single-particle cryo-EM. We also review the status of using pulsed or near-field enhanced laser light as alternatives, along with approaches that use scanning transmission electron microscopy (STEM) with a segmented detector rather than a phase plate

Observation of the relativistic reversal of the ponderomotive potential

The secular dynamics of a non-relativistic charged particle in an electromagnetic wave can be described by the ponderomotive potential. Although ponderomotive electron-laser interactions at relativistic velocities are important for emerging technologies from laser-based particle accelerators to laser-enhanced electron microscopy, the effects of special relativity on the interaction have only been studied theoretically. Here, we use a transmission electron microscope to measure the position-dependent phase shift imparted to a relativistic electron wave function when it traverses a standing laser wave. The kinetic energy of the electrons is varied between $80\,\mathrm{keV}$ and $300\,\mathrm{keV}$, and the laser standing wave has a continuous-wave intensity of $175\,\mathrm{GW/cm}^2$. In contrast to the non-relativistic case, we demonstrate that the phase shift depends on both the electron velocity and the wave polarization, confirming the predictions of a quasiclassical theory of the interaction. Remarkably, if the electron's speed is greater than $1/\sqrt{2}$ of the speed of light, the phase shift at the electric field nodes of the wave can exceed that at the antinodes. In this case there exists a polarization such that the phase shifts at the nodes and antinodes are equal, and the electron does not experience Kapitza-Dirac diffraction. Our results thus provide new capabilities for coherent electron beam manipulation

Association of Center Volume with Outcome After Liver and Kidney Transplantation

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73934/1/j.1600-6143.2004.00462.x.pd

Supersymmetric Electroweak Corrections to Charged Higgs Boson Production in Association with a Top Quark at Hadron Colliders

We calculate the $O(\alpha_{ew}m_{t(b)}^{2}/m_{W}^{2})$ and $O(\alpha_{ew} m_{t(b)}^4/m_W^4)$ supersymmetric electroweak corrections to the cross section for the charged Higgs boson production in association with a top quark at the Tevatron and the LHC. These corrections arise from the quantum effects which are induced by potentially large Yukawa couplings from the Higgs sector and the chargino-top(bottom)-sbottom(stop) couplings, neutralino-top(bottom)-stop(sbottom) couplings and charged Higgs-stop-sbottom couplings. They can decrease or increase the cross section depending on $\tan\beta$ but are not very sensitive to the mass of the charged Higgs boson for high $\tan\beta$. At low $\tan\beta(=2)$ the corrections decrease the total cross sections significantly, which exceed -12% for $m_{H^{\pm}}$ below $300GeV$ at both the Tevatron and the LHC, but for $m_{H^{\pm}}>300GeV$ the corrections can become very small at the LHC. For high $\tan\beta(=10,30)$ these corrections can decrease or increase the total cross sections, and the magnitude of the corrections are at most a few percent at both the Tevatron and the LHC.Comment: 28 pages including 4 eps figure

Supersymmetric effects in top quark decay into polarized W-boson

We investigate the one-loop supersymmetric QCD (SUSY-QCD) and electroweak (SUSY-EW) corrections to the top quark decay into a b-quark and a longitudinal or transverse W-boson. The corrections are presented in terms of the longitudinal ratio \Gamma(t-->W_L b)/\Gamma(t--> W b) and the transverse ratio \Gamma(t-->W_- b)/\Gamma(t--> W b). In most of the parameter space, both SUSY-QCD and SUSY-EW corrections to these ratios are found to be less than 1% in magnitude and they tend to have opposite signs. The corrections to the total width \Gamma(t-->W b) are also presented for comparison with the existing results in the literature. We find that our SUSY-EW corrections to the total width differ significantly from previous studies: the previous studies give a large correction of more than 10% in magnitude for a large part of the parameter space while our results reach only few percent at most.Comment: Version in PRD (explanation and refs added

The Low-Velocity, Rapidly Fading Type Ia Supernova 2002es

SN 2002es is a peculiar subluminous Type Ia supernova (SN Ia) with a combination of observed characteristics never before seen in a SN Ia. At maximum light, SN 2002es shares spectroscopic properties with the underluminous SN 1991bg subclass of SNe Ia, but with substantially lower expansion velocities (~6000 km/s) more typical of the SN 2002cx subclass. Photometrically, SN 2002es differs from both SN 1991bg-like and SN 2002cx-like supernovae. Although at maximum light it is subluminous (M_B=-17.78 mag), SN 2002es has a relatively broad light curve (Dm15(B)=1.28 +/- 0.04 mag), making it a significant outlier in the light-curve width vs. luminosity relationship. We estimate a 56Ni mass of 0.17 +/- 0.05 M_sun synthesized in the explosion, relatively low for a SN Ia. One month after maximum light, we find an unexpected plummet in the bolometric luminosity. The late-time decay of the light curves is inconsistent with our estimated 56Ni mass, indicating that either the light curve was not completely powered by 56Ni decay or the ejecta became optically thin to gamma-rays within a month after maximum light. The host galaxy is classified as an S0 galaxy with little to no star formation, indicating the progenitor of SN 2002es is likely from an old stellar population. We also present a less extensive dataset for SN 1999bh, an object which shares similar observed properties. Both objects were found as part of the Lick Observatory Supernova Search, allowing us to estimate that these objects should account for ~2.5% of SNe Ia within a fixed volume. We find that current theoretical models are unable to explain the observed of characteristics of SN 2002es.Comment: 19 pages, 15 figures, Submitted to Ap

Axiomatic quantum field theory in curved spacetime

The usual formulations of quantum field theory in Minkowski spacetime make crucial use of features--such as Poincare invariance and the existence of a preferred vacuum state--that are very special to Minkowski spacetime. In order to generalize the formulation of quantum field theory to arbitrary globally hyperbolic curved spacetimes, it is essential that the theory be formulated in an entirely local and covariant manner, without assuming the presence of a preferred state. We propose a new framework for quantum field theory, in which the existence of an Operator Product Expansion (OPE) is elevated to a fundamental status, and, in essence, all of the properties of the quantum field theory are determined by its OPE. We provide general axioms for the OPE coefficients of a quantum field theory. These include a local and covariance assumption (implying that the quantum field theory is locally and covariantly constructed from the spacetime metric), a microlocal spectrum condition, an "associativity" condition, and the requirement that the coefficient of the identity in the OPE of the product of a field with its adjoint have positive scaling degree. We prove curved spacetime versions of the spin-statistics theorem and the PCT theorem. Some potentially significant further implications of our new viewpoint on quantum field theory are discussed.Comment: Latex, 44 pages, 2 figure

In and Out of Equilibrium II: Evolution in Repeated Games with Discounting and Complexity Costs

We explore evolutionary dynamics for repeated games with small, but positive complexity costs. To understand the dynamics, we extend a folk theorem result by Cooper (1996) to continuation probabilities, or discount rates, smaller than 1. While this result delineates which payoffs can be supported by neutrally stable strategies, the only strategy that is evolutionarily stable, and has a uniform invasion barrier, is All D. However, with sufficiently small complexity costs, indirect invasions - but now through 'almost neutral' mutants - become an important ingredient of the dynamics. These indirect invasions include stepping stone paths out of full defection

The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function.

Proteins that contain the DUF2874 domain constitute a new Pfam family PF11396. Members of this family have predominantly been identified in microbes found in the human gut and oral cavity. The crystal structure of one member of this family, BVU2987 from Bacteroides vulgatus, has been determined, revealing a β-lactamase inhibitor protein-like structure with a tandem repeat of domains. Sequence analysis and structural comparisons reveal that BVU2987 and other DUF2874 proteins are related to β-lactamase inhibitor protein, PepSY and SmpA_OmlA proteins and hence are likely to function as inhibitory proteins