ERBE/NOAA-9 observations for the FIRE/SRB Wisconsin experiment region from October 14 through October 31, 1986

Tables are presented which show ERBE/NOAA-9 radiances, resolution size, and pixel location for the FIRE/SRB Wisconsin experiment region from October 1 through October 31, l986. Also presented is an analysis of the ERBE point spread function used to estimate pixel resolution size for the scanner instrument

Monensin causes dose dependent inhibition of Mycobacterium avium subspecies paratuberculosis in radiometric culture

<p>Abstract</p> <p>Background</p> <p><it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>(MAP) causes a chronic wasting diarrheal disease in ruminants called Johne's disease, that is evocative of human inflammatory bowel disease (IBD). Agents used to treat IBD, called "anti-inflammatories", immuno-modulators" and "immuno-suppressants" inhibit MAP growth in culture. We concluded that, unknowingly, the medical profession has been treating MAP since sulfasalazine's introduction in 1942. Monensin, called a "Growth Enhancer" in cattle, ameliorates Johne's disease without a documented mechanism of action. We hypothesized that Monensin would inhibit MAP in culture.</p> <p>Methods</p> <p>Using the radiometric ¹⁴CO₂Bactec^®system, that expresses mycobacterial growth in arbitrary growth index (GI) units, we studied the effect of Monensin on the growth kinetic of MAP isolated from humans with IBD ("Dominic", "Ben" & UCF-4) and cattle with Johne's disease (303 & ATCC 19698.) Results are expressed as percent inhibition of cumulative GI (%–ΔcGI).</p> <p>Results</p> <p>The positive control Clofazimine inhibits every strain tested. The negative controls Cycloheximide & Phthalimide, have no inhibition on any MAP strain. Monensin has dose dependent inhibition on every MAP strain tested. The most susceptible human isolate was UCF-4 (73% – ΔcGI at 1 μg/ml) and bovine isolate was 303 (73% – ΔcGI at 4 μg/ml.) Monensin additionally inhibits <it>M. avium </it>ATCC 25291 (87% – ΔcGI at 64 μg/ml) & BCG (92% – ΔcGI at 16 μg/ml).</p> <p>Discussion</p> <p>We show that in radiometric culture the "Growth Enhancer" Monensin causes dose dependent inhibition of mycobacteria including MAP. We posit that the "Growth Enhancer" effect of Monensin may, at least in part, be due to inhibition of MAP in clinical or sub-clinical Johne's disease.</p

Cloud fraction, layer, and direction of movement results from sky cameras during the FIRE IFO, Coffeyville, Kansas, experiment for the period Nov. 12 through Dec. 9, 1991

Tables and figures are presented which show local site observations of cloud fractions, the number of cloud layers, direction of movement, and precipitation data collected during the FIRE (First ISCCP Regional Experiment) Phase 2 Cirrus Intensive Field Observations (IFO) conducted in Coffeyville, Kansas during November and December, 1991. Selected data are also presented at the times of the TIROS Operational Vertical Sounder (TOVS) satellite overpass. Several major scientific projects have used surface-based observations of clouds to compare directly with those being observed from satellites. Characterizing the physical properties of clouds is extremely useful in obtaining a more accurate analysis of the effect of clouds and their movements on weather and climate. It is the purpose of this paper to report data collected during the FIRE Phase 2 IFO experiment and to provide a brief history of such a surface-based system and the technical information required for recording local cloud parameters

An experimental approach for investigating many-body phenomena in Rydberg-interacting quantum systems

Recent developments in the study of ultracold Rydberg gases demand an advanced level of experimental sophistication, in which high atomic and optical densities must be combined with excellent control of external fields and sensitive Rydberg atom detection. We describe a tailored experimental system used to produce and study Rydberg-interacting atoms excited from dense ultracold atomic gases. The experiment has been optimized for fast duty cycles using a high flux cold atom source and a three beam optical dipole trap. The latter enables tuning of the atomic density and temperature over several orders of magnitude, all the way to the Bose-Einstein condensation transition. An electrode structure surrounding the atoms allows for precise control over electric fields and single-particle sensitive field ionization detection of Rydberg atoms. We review two experiments which highlight the influence of strong Rydberg--Rydberg interactions on different many-body systems. First, the Rydberg blockade effect is used to pre-structure an atomic gas prior to its spontaneous evolution into an ultracold plasma. Second, hybrid states of photons and atoms called dark-state polaritons are studied. By looking at the statistical distribution of Rydberg excited atoms we reveal correlations between dark-state polaritons. These experiments will ultimately provide a deeper understanding of many-body phenomena in strongly-interacting regimes, including the study of strongly-coupled plasmas and interfaces between atoms and light at the quantum level.Comment: 14 pages, 11 figures; submitted to a special issue of 'Frontiers of Physics' dedicated to 'Quantum Foundation and Technology: Frontiers and Future

Wave Climate Model of the Mid-Atlantic Shelf and Shoreline (Virginia Sea): Model Development, Shelf Geomorphology, and Preliminary Results

A computerized wave climate model is developed that applies linear wave theory and shelf depth information to predict wave behavior as they pass over the continental shelf as well as the resulting wave energy distributions along the coastline. Reviewed are also the geomorphology of the Mid-Atlantic Continental Shelf, wave computations resulting from 122 wave input conditions, and a preliminary analysis of these data

Full counting statistics of laser excited Rydberg aggregates in a one-dimensional geometry

We experimentally study the full counting statistics of few-body Rydberg aggregates excited from a quasi-one-dimensional Rydberg gas. We measure asymmetric excitation spectra and increased second and third order statistical moments of the Rydberg number distribution, from which we determine the average aggregate size. Direct comparisons with numerical simulations reveal the presence of liquid-like spatial correlations, and indicate sequential growth of the aggregates around an initial grain. These findings demonstrate the importance of dissipative effects in strongly correlated Rydberg gases and introduce a way to study spatio-temporal correlations in strongly-interacting many-body quantum systems without imaging.Comment: 6 pages plus supplemen

Interaction enhanced imaging of individual atoms embedded in dense atomic gases

We propose a new all-optical method to image individual atoms within dense atomic gases. The scheme exploits interaction induced shifts on highly polarizable excited states, which can be spatially resolved via an electromagnetically induced transparency resonance. We focus in particular on imaging strongly interacting many-body states of Rydberg atoms embedded in an ultracold gas of ground state atoms. Using a realistic model we show that it is possible to image individual impurity atoms with enhanced sensitivity and high resolution despite photon shot noise and atomic density fluctuations. This new imaging scheme is ideally suited to equilibrium and dynamical studies of complex many-body phenomena involving strongly interacting atoms. As an example we study blockade effects and correlations in the distribution of Rydberg atoms optically excited from a dense gas.Comment: 5 pages plus supplementary materia

Evolution of the Bovine TLR Gene Family and Member Associations with Mycobacterium avium Subspecies paratuberculosis Infection

Members of the Toll-like receptor (TLR) gene family occupy key roles in the mammalian innate immune system by functioning as sentries for the detection of invading pathogens, thereafter provoking host innate immune responses. We utilized a custom next-generation sequencing approach and allele-specific genotyping assays to detect and validate 280 biallelic variants across all 10 bovine TLR genes, including 71 nonsynonymous single nucleotide polymorphisms (SNPs) and one putative nonsense SNP. Bayesian haplotype reconstructions and median joining networks revealed haplotype sharing between Bos taurus taurus and Bos taurus indicus breeds at every locus, and specialized beef and dairy breeds could not be differentiated despite an average polymorphism density of 1 marker/158 bp. Collectively, 160 tagSNPs and two tag insertion-deletion mutations (indels) were sufficient to predict 100% of the variation at 280 variable sites for both Bos subspecies and their hybrids, whereas 118 tagSNPs and 1 tagIndel predictively captured 100% of the variation at 235 variable sites for B. t. taurus. Polyphen and SIFT analyses of amino acid (AA) replacements encoded by bovine TLR SNPs indicated that up to 32% of the AA substitutions were expected to impact protein function. Classical and newly developed tests of diversity provide strong support for balancing selection operating on TLR3 and TLR8, and purifying selection acting on TLR10. An investigation of the persistence and continuity of linkage disequilibrium (r2≥0.50) between adjacent variable sites also supported the presence of selection acting on TLR3 and TLR8. A case-control study employing validated variants from bovine TLR genes recognizing bacterial ligands revealed six SNPs potentially eliciting small effects on susceptibility to Mycobacterium avium spp paratuberculosis infection in dairy cattle. The results of this study will broadly impact domestic cattle research by providing the necessary foundation to explore several avenues of bovine translational genomics, and the potential for marker-assisted vaccination