Testicular cancer and sperm DNA damage: short- and long-term effects of antineoplastic treatment

The aim of this study was to investigate sperm DNA damage induced by chemo- and radiotherapy in patients with testicular cancer to provide data on the extent and persistence of nuclear damage that might affect individual reproductive potential. We evaluated pre- and post-antineoplastic treatment sperm DNA integrity, expressed as DNA Fragmentation Index (DFI), in a large caseload of testicular cancer patients by sperm chromatin structure assay. The mean total DFI for all patients at T0 was 18.0 ± 12.5%. Sperm chromatin profile was markedly impaired at T3 (27.7 ± 17.4%) and T6 (23.2 ± 15.3%), improving considerably at T12 and T24 (14.0 ± 8.9% and 14.4 ± 10.3%). After chemotherapy, we found a marked increase in DFI at T3 and T6 and a significant reduction at T12 and T24 in comparison with the baseline. In contrast, DFI increased at T3 and T6 after radiotherapy but the subsequent reduction was far less marked, reaching baseline values at T12 and T24. Finally, post-treatment DNA damage was not age or histotype dependent, but was more marked in the advanced stage of cancer. In this study, we showed that the chromatin profile may be affected in the months immediately following the end of the treatment, improving after 12-24 months. Our results thus indicate that post-treatment DNA damage is influenced both by the type and intensity of the therapy and by the pathological and clinical stage of the disease. © 2014 American Society of Andrology and European Academy of Andrology

Subsurface characterization of groundwater contaminated by landfill leachate using microbial community profile data and a nonparametric decision-making process

Microbial biodiversity in groundwater and soil presents a unique opportunity for improving characterization and monitoring at sites with multiple contaminants, yet few computational methods use or incorporate these data because of their high dimensionality and variability. We present a systematic, nonparametric decision-making methodology to help characterize a water quality gradient in leachate-contaminated groundwater using only microbiological data for input. The data-driven methodology is based on clustering a set of molecular genetic-based microbial community profiles. Microbes were sampled from groundwater monitoring wells located within and around an aquifer contaminated with landfill leachate. We modified a self-organizing map (SOM) to weight the input variables by their relative importance and provide statistical guidance for classifying sample similarities. The methodology includes the following steps: (1) preprocessing the microbial data into a smaller number of independent variables using principal component analysis, (2) clustering the resulting principal component (PC) scores using a modified SOM capable of weighting the input PC scores by the percent variance explained by each score, and (3) using a nonparametric statistic to guide selection of appropriate groupings for management purposes. In this landfill leachate application, the weighted SOM assembles the microbial community data from monitoring wells into groupings believed to represent a gradient of site contamination that could aid in characterization and long-term monitoring decisions. Groupings based solely on microbial classifications are consistent with classifications of water quality from hydrochemical information. These microbial community profile data and improved decision-making strategy compliment traditional chemical groundwater analyses for delineating spatial zones of groundwater contamination. © 2011 by the American Geophysical Union

Autothermal biochar production and characterization at pilot scale

The present work reports on the results from the validation campaign of an autothermal pilot carbonization unit (CarbON) and on the characterization of the produced biochar and pyrolysis vapors. The proposed pilot plant leverages the simplicity and effectiveness of autothermal operations together with open top, downdraft design, to bring to the small scale the performance of larger installations. In autothermal operation, heat for the process is internally provided by combusting part of the feedstock and evolved volatiles inside the reactor, the so called “oxidative pyrolysis”. Please click on the file below for full content of the abstract

Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane

<p>Abstract</p> <p>Background</p> <p>PUFAs are important molecules for membrane order and function; they can modify inflammation-inducible cytokines production, eicosanoid production, plasma triacylglycerol synthesis and gene expression. Recent studies suggest that n-3 PUFAs can be cancer chemopreventive, chemosuppressive and auxiliary agents for cancer therapy. N-3 PUFAs could alter cancer growth influencing cell replication, cell cycle, and cell death. The question that remains to be answered is how n-3 PUFAs can affect so many physiological processes. We hypothesize that n-3 PUFAs alter membrane stability, modifying cellular signalling in breast cancer cells.</p> <p>Methods</p> <p>Two lines of human breast cancer cells characterized by different expression of ER and EGFR receptors were treated with AA, EPA or DHA. We have used the MTT viability test and expression of apoptotic markers to evaluate the effect of PUFAs on cancer growth. Phospholipids were analysed by HPLC/GC, to assess n-3 incorporation into the cell membrane.</p> <p>Results</p> <p>We have observed that EPA and DHA induce cell apoptosis, a reduction of cell viability and the expression of Bcl2 and procaspase-8. Moreover, DHA slightly reduces the concentration of EGFR but EPA has no effect. Both EPA and DHA reduce the activation of EGFR.</p> <p>N-3 fatty acids are partially metabolized in both cell lines; AA is integrated without being further metabolized. We have analysed the fatty acid pattern in membrane phospholipids where they are incorporated with different degrees of specificity. N-3 PUFAs influence the n-6 content and vice versa.</p> <p>Conclusions</p> <p>Our results indicate that n-3 PUFA feeding might induce modifications of breast cancer membrane structure that increases the degree of fatty acid unsaturation. This paper underlines the importance of nutritional factors on health maintenance and on disease prevention.</p

Instability of one-step replica-symmetry-broken phase in satisfiability problems

We reconsider the one-step replica-symmetry-breaking (1RSB) solutions of two random combinatorial problems: k-XORSAT and k-SAT. We present a general method for establishing the stability of these solutions with respect to further steps of replica-symmetry breaking. Our approach extends the ideas of [A.Montanari and F. Ricci-Tersenghi, Eur.Phys.J. B 33, 339 (2003)] to more general combinatorial problems. It turns out that 1RSB is always unstable at sufficiently small clauses density alpha or high energy. In particular, the recent 1RSB solution to 3-SAT is unstable at zero energy for alpha< alpha_m, with alpha_m\approx 4.153. On the other hand, the SAT-UNSAT phase transition seems to be correctly described within 1RSB.Comment: 26 pages, 7 eps figure

The sustainability of old grapevine mother plants in relation to new mandatory diagnostic tests for virus control

In 2011, the methods to perform phytosanitary tests to check for viruses in grapevine nurseries were reassessed and new regulations were defined (DM 13 December 2011). The mandatory tests require serological assays for the diagnosis of five viruses in grapevine mother plants transplanted in Tuscany in 2001 or before. The aim of the present paper is to report the impact of certification programs applied before 2001 in Tuscany and the sustainability of older mother plants with relation to the new mandatory diagnostic tests. Among the cultivars, virus infection was reported in 19.2% of pool samples, whereas 2.4% of rootstock pool samples showed a compromised health status. GLRaV-3 is the most frequently found virus (10.4% and 1.3% of cultivar and rootstock pools, respectively), and it is also included in the most frequent multiple infections. Multiple infections represent about 25% of infected cultivar pools and almost 50% of infected rootstock pools

Comparing properties of natural biogenic with biomass burning particles in Amazonia.

The Large Scale Biosphere Atmosphere Experiment in\ud Amazonia (LBA) is a long-term (20 years) research effort\ud aimed at the understanding of the functioning of the\ud Amazonian ecosystem. The strong biosphere-atmosphere\ud interaction is a key component of the ecosystem functioning.\ud Two aerosol components are the most visible: The natural\ud biogenic emissions of particles and VOCs, and the biomass\ud burning emissions.\ud Two aerosol and trace gases monitoring stations were\ud operated for 4 years in Manaus and Porto Velho, two very\ud distinct sites, with different land use change. Manaus is a very\ud clean and pristine site and Porto Velho is representative of\ud heavy land use change in Amazonia. Aerosol composition,\ud optical properties, size distribution, vertical profiling and\ud optical depth were measured from 2008 to 2012. Aerosol\ud radiative forcing was calculated over large areas. It was\ud observed that the natural biogenic aerosol has significant\ud absorption properties. Organic aerosol dominates the aerosol\ud mass with 80 to 95%. Light scattering and light absorption\ud shows an increase by factor of 10 from Manaus to Porto\ud Velho. Very few new particle formation events were\ud observed. Strong links between aerosols and VOC emissions\ud were observed. Aerosol radiative forcing in Rondonia shows\ud a high -15 watts/m² during the dry season of 2010, showing\ud the large impacts of aerosol loading in the Amazonian\ud ecosystem. The increase in diffuse radiation changes the\ud forest carbon uptake by 20 to 35%, a large increase in this\ud important ecosystem

Long term measurements of aerosol optical properties at a primary forest site in Amazonia

A long term experiment was conducted in a primary forest area in Amazonia, with continuous in-situ measurements of aerosol optical properties between February 2008 and April 2011, comprising, to our knowledge, the longest database ever in the Amazon Basin. Two major classes of aerosol particles, with significantly different optical properties were identified: coarse mode predominant biogenic aerosols in the wet season (January-June), naturally released by the forest metabolism, and fine mode dominated biomass burning aerosols in the dry season (July-December), transported from regional fires. Dry particle median scattering coefficients at the wavelength of 550 nm increased from 6.3 Mm(-1) to 22 Mm(-1), whereas absorption at 637 nm increased from 0.5 Mm(-1) to 2.8 Mm(-1) from wet to dry season. Most of the scattering in the dry season was attributed to the predominance of fine mode (PM2) particles (40-80% of PM10 mass), while the enhanced absorption coefficients are attributed to the presence of light absorbing aerosols from biomass burning. As both scattering and absorption increased in the dry season, the single scattering albedo (SSA) did not show a significant seasonal variability, in average 0.86 +/- 0.08 at 637 nm for dry aerosols. Measured particle optical properties were used to estimate the aerosol forcing efficiency at the top of the atmosphere. Results indicate that in this primary forest site the radiative balance was dominated by the cloud cover, particularly in the wet season. Due to the high cloud fractions, the aerosol forcing efficiency absolute values were below -3.5 Wm(-2) in 70% of the wet season days and in 46% of the dry season days. Besides the seasonal variation, the influence of out-of-Basin aerosol sources was observed occasionally. Periods of influence of the Manaus urban plume were detected, characterized by a consistent increase on particle scattering (factor 2.5) and absorption coefficients (factor 5). Episodes of biomass burning and mineral dust particles advected from Africa were observed between January and April, characterized by enhanced concentrations of crustal elements (Al, Si, Ti, Fe) and potassium in the fine mode. During these episodes, median particle absorption coefficients increased by a factor of 2, whereas median SSA values decreased by 7 %, in comparison to wet season conditions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)European Integrated FP6 project on Aerosol Cloud Climate and Air Quality Interactions (EUCAARI) under the scope of LBA experimentUniversidade Federal de São Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Earth & Exact Sci, São Paulo, BrazilUniv São Paulo, Inst Phys, Dept Appl Phys, São Paulo, BrazilLeibniz Inst Tropospher Res, Leipzig, GermanyNatl Inst Amazonian Res INPA, Manaus, Amazonas, BrazilLund Univ, Inst Phys, Div Nucl Phys, Lund, SwedenHarvard Univ, Dept Earth & Planetary Sci, Div Engn & Appl Sci, Cambridge, MA 02138 USAUniv Helsinki, Div Atmospher Sci, Dept Phys Sci, Helsinki, FinlandUniversidade Federal de São Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Earth & Exact Sci, São Paulo, BrazilFAPESP: AEROCLIMA 08/58100-2European Integrated FP6 project on Aerosol Cloud Climate and Air Quality Interactions (EUCAARI) under the scope of LBA experiment: 34684Web of Scienc