A Critical Study of Youth and Youth Work and a Workable Plan of Youth Evangelism for the Local Church

The youth of the United States of America have fallen to the lowest moral and spiritual level of any generation of youth which has yet lived in this country. Never before was there a time when such a majority of the criminals were of the adolescent and youth age. Secular authorities along with moralists and religious leaders are alarmed and are searching earnestly for an answer to the problems of juvenile delinquency. The glamorous advertising of liquor, superemphasizing of sex, glorification of materialism, degeneration of the home, along with numerous other anti-spiritual emphases of the past decade, has produced a generation of youth whose basic philosophy is to eat, drink and be merry

Proposed Repositioning of the Pennsylvanian-Permian Boundary in Kansas

The Pennsylvanian-Permian boundary in North America has not corresponded with the Carboniferous-Permian boundary in Europe for decades. To facilitate global correlations, an attempt is here made to suggest a possible solution to the dilemma by making the best possible correlation of the Kansas stratigraphic section with the recently proposed boundary location in the Russian type section. The Virgilian Stage (Upper Pennsylvanian) was defined nearly 60 years ago to include those rocks lying between the Missourian Stage and the base of the Permian System. In the type area in east-central Kansas, the Virgilian Stage comprised the Douglas, Shawnee, and Wabaunsee Groups. In Kansas, the Pennsylvanian-Permian boundary was placed eventually at the top of the Brownville Limestone Member on the basis of what was then believed to be a regional disconformity rather than on paleontological criteria. Recent advances in fusulinid and conodont biostratigraphy provide tentative criteria upon which to suggest a change in the placement of the Virgilian-Permian boundary. A Russian delegation formally proposed at the International Congress on the Permian System of the World held in Perm, U.S.S.R. (Russia) in August 1991 that the base of the Permian System be established at the base of the Asselian Stage at the approximate stratigraphic position of the first inflated fusulinids (Sphaeroschwagerina vulgaris-S. fusiformis). Inflated schwagerinids (Paraschwagerina kansasensis) first occur, along with evolutionary changes in conodonts, in the Neva Limestone Member of the Grenola Limestone (Council Grove Group). Thus, if we assume that inflated schwagerinids arose globally at about the same time, the Neva Limestone Member is the oldest definitive Permian in the United States midcontinent, as related to the newly proposed boundary in Russia and Kazakhstan. Consequently, we propose that the Virgilian Stage in Kansas include rocks between the top of the Missourian Stage and the base of the Neva Limestone Member

Redefinition of the Upper Pennsylvanian Virgilian Series in Kansas

The Virgilian Series was defined nearly 60 years ago to include those rocks lying between the Missourian Series and the base of the Permian System. In the type area in east-central Kansas, the Virgilian Series comprised the Douglas, Shawnee, and Wabaunsee Groups. In Kansas, the upper boundary of the Virgilian (Pennsylvanian-Permian boundary) was placed at the top of the Brownville Limestone Member on the basis of what was then believed to be a regional disconformity rather than on paleontological criteria. Recent advances in fusulinid and conodont biostratigraphy provide tentative criteria upon which to effect a change in the placement of the Virgilian-Permian boundary. It is now generally agreed that the base of the Permian System is approximated by the first occurrence of Pseudoschwagerina, an inflated schwagerinid. Furthermore, the Subcommission on Permian Stratigraphy has informally agreed that the base of the Permian should coincide with the first occurrence of the conodont species Streptognathodus barskovi. Inflated schwagerinids (Paraschwagerina kansasensis) first occur along with evolutionary changes in the Conodonta in the Neva Limestone of the Council Grove Group. Consequently, the Virgilian Series is herein redefined to include rocks present between the top of the Missourian Series and the base of the Neva Limestone. To increase compatibility between chronostratigraphic and lithostratigraphic nomenclature, the following changes are made: I) the Admire Group is redefined to include rocks between the base of the Onaga Shale and the base of the Neva Limestone; 2) the Admire is reassigned to the upper Virgilian Series; 3) the Neva Limestone is elevated to formational status; 4) the Grenola Limestone is redefined to include strata between the top of the Roca Shale and the base of the Neva Limestone; 5) the overlying Council Grove Group is redefined to include strata lying between the base of the Neva Limestone and the base of the Chase Group; and 6) regionally the base of the emended Council Grove Group marks the base of the Permian System. The emended Council Grove Group is lower Wolfcampian in age and is time equivalent with the Neal Ranch Formation of the west Texas type Wolfcampian

Redefinition of the Upper Pennsylvanian Virgilian Series in Kansas

The Virgilian Series was defined nearly 60 years ago to include those rocks lying between the Missourian Series and the base of the Permian System. In the type area in east-central Kansas, the Virgilian Series comprised the Douglas, Shawnee, and Wabaunsee Groups. In Kansas, the upper boundary of the Virgilian (Pennsylvanian-Permian boundary) was placed at the top of the Brownville Limestone Member on the basis of what was then believed to be a regional disconformity rather than on paleontological criteria. Recent advances in fusulinid and conodont biostratigraphy provide tentative criteria upon which to effect a change in the placement of the Virgilian-Permian boundary. It is now generally agreed that the base of the Permian System is approximated by the first occurrence of Pseudoschwagerina, an inflated schwagerinid. Furthermore, the Subcommission on Permian Stratigraphy has informally agreed that the base of the Permian should coincide with the first occurrence of the conodont species Streptognathodus barskovi. Inflated schwagerinids (Paraschwagerina kansasensis) first occur along with evolutionary changes in the Conodonta in the Neva Limestone of the Council Grove Group. Consequently, the Virgilian Series is herein redefined to include rocks present between the top of the Missourian Series and the base of the Neva Limestone. To increase compatibility between chronostratigraphic and lithostratigraphic nomenclature, the following changes are made: I) the Admire Group is redefined to include rocks between the base of the Onaga Shale and the base of the Neva Limestone; 2) the Admire is reassigned to the upper Virgilian Series; 3) the Neva Limestone is elevated to formational status; 4) the Grenola Limestone is redefined to include strata between the top of the Roca Shale and the base of the Neva Limestone; 5) the overlying Council Grove Group is redefined to include strata lying between the base of the Neva Limestone and the base of the Chase Group; and 6) regionally the base of the emended Council Grove Group marks the base of the Permian System. The emended Council Grove Group is lower Wolfcampian in age and is time equivalent with the Neal Ranch Formation of the west Texas type Wolfcampian

Proposed Repositioning of the Pennsylvanian-Permian Boundary in Kansas

The Pennsylvanian-Permian boundary in North America has not corresponded with the Carboniferous-Permian boundary in Europe for decades. To facilitate global correlations, an attempt is here made to suggest a possible solution to the dilemma by making the best possible correlation of the Kansas stratigraphic section with the recently proposed boundary location in the Russian type section. The Virgilian Stage (Upper Pennsylvanian) was defined nearly 60 years ago to include those rocks lying between the Missourian Stage and the base of the Permian System. In the type area in east-central Kansas, the Virgilian Stage comprised the Douglas, Shawnee, and Wabaunsee Groups. In Kansas, the Pennsylvanian-Permian boundary was placed eventually at the top of the Brownville Limestone Member on the basis of what was then believed to be a regional disconformity rather than on paleontological criteria. Recent advances in fusulinid and conodont biostratigraphy provide tentative criteria upon which to suggest a change in the placement of the Virgilian-Permian boundary. A Russian delegation formally proposed at the International Congress on the Permian System of the World held in Perm, U.S.S.R. (Russia) in August 1991 that the base of the Permian System be established at the base of the Asselian Stage at the approximate stratigraphic position of the first inflated fusulinids (Sphaeroschwagerina vulgaris-S. fusiformis). Inflated schwagerinids (Paraschwagerina kansasensis) first occur, along with evolutionary changes in conodonts, in the Neva Limestone Member of the Grenola Limestone (Council Grove Group). Thus, if we assume that inflated schwagerinids arose globally at about the same time, the Neva Limestone Member is the oldest definitive Permian in the United States midcontinent, as related to the newly proposed boundary in Russia and Kazakhstan. Consequently, we propose that the Virgilian Stage in Kansas include rocks between the top of the Missourian Stage and the base of the Neva Limestone Member

UGT1A1 is a major locus influencing bilirubin levels in African Americans

Total serum bilirubin is associated with several clinical outcomes, including cardiovascular disease, diabetes and drug metabolism. We conducted a genome-wide association study in 619 healthy unrelated African Americans in an attempt to replicate reported findings in Europeans and Asians and to identify novel loci influencing total serum bilirubin levels. We analyzed a dense panel of over two million genotyped and imputed SNPs in additive genetic models adjusting for age, sex, and the first two significant principal components from the sample covariance matrix of genotypes. Thirty-nine SNPs spanning a 78 kb region within the UGT1A1 displayed P-values <5 × 10−8. The lowest P-value was 1.7 × 10−22 for SNP rs887829. None of SNPs in the UGT1A1 remained statistically significant in conditional association analyses that adjusted for rs887829. In addition, SNP rs10929302 located in phenobarbital response enhancer module was significantly associated with bilirubin level with a P-value of 1.37 × 10−11; this enhancer module is believed to have a critical role in phenobarbital treatment of hyperbilirubinemia. Interestingly, the lead SNP, rs887829, is in strong linkage disequilibrium (LD) (r2≥0.74) with rs10929302. Taking advantage of the lower LD and shorter haplotypes in African-ancestry populations, we identified rs887829 as a more refined proxy for the causative variant influencing bilirubin levels. Also, we replicated the reported association between variants in SEMA3C and bilirubin levels. In summary, UGT1A1 is a major locus influencing bilirubin levels and the results of this study promise to contribute to understanding of the etiology and treatment of hyperbilirubinaemia in African-ancestry populations

A recurrent germline PAX5 mutation confers susceptibility to pre-B cell acute lymphoblastic leukemia

Somatic alterations of the lymphoid transcription factor gene PAX5 (also known as BSAP) are a hallmark of B cell precursor acute lymphoblastic leukemia (B-ALL)1–3, but inherited mutations of PAX5 have not previously been described. Here we report a new heterozygous germline variant, c.547G>A (p.Gly183Ser), affecting the octapeptide domain of PAX5 that was found to segregate with disease in two unrelated kindreds with autosomal dominant B-ALL. Leukemic cells from all affected individuals in both families exhibited 9p deletion, with loss of heterozygosity and retention of the mutant PAX5 allele at 9p13. Two additional sporadic ALL cases with 9p loss harbored somatic PAX5 substitutions affecting Gly183. Functional and gene expression analysis of the PAX5 mutation demonstrated that it had significantly reduced transcriptional activity. These data extend the role of PAX5 alterations in the pathogenesis of pre-B cell ALL and implicate PAX5 in a new syndrome of susceptibility to pre-B cell neoplasia

The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death

Plant NB-LRR proteins confer robust protection against microbes and metazoan parasites by recognizing pathogen-derived avirulence (Avr) proteins that are delivered to the host cytoplasm. Microbial Avr proteins usually function as virulence factors in compatible interactions; however, little is known about the types of metazoan proteins recognized by NB-LRR proteins and their relationship with virulence. In this report, we demonstrate that the secreted protein RBP-1 from the potato cyst nematode Globodera pallida elicits defense responses, including cell death typical of a hypersensitive response (HR), through the NB-LRR protein Gpa2. Gp-Rbp-1 variants from G. pallida populations both virulent and avirulent to Gpa2 demonstrated a high degree of polymorphism, with positive selection detected at numerous sites. All Gp-RBP-1 protein variants from an avirulent population were recognized by Gpa2, whereas virulent populations possessed Gp-RBP-1 protein variants both recognized and non-recognized by Gpa2. Recognition of Gp-RBP-1 by Gpa2 correlated to a single amino acid polymorphism at position 187 in the Gp-RBP-1 SPRY domain. Gp-RBP-1 expressed from Potato virus X elicited Gpa2-mediated defenses that required Ran GTPase-activating protein 2 (RanGAP2), a protein known to interact with the Gpa2 N terminus. Tethering RanGAP2 and Gp-RBP-1 variants via fusion proteins resulted in an enhancement of Gpa2-mediated responses. However, activation of Gpa2 was still dependent on the recognition specificity conferred by amino acid 187 and the Gpa2 LRR domain. These results suggest a two-tiered process wherein RanGAP2 mediates an initial interaction with pathogen-delivered Gp-RBP-1 proteins but where the Gpa2 LRR determines which of these interactions will be productive