Phase Coherence in Wind Data and Simulation

<p>Novel wind turbine designs are deemed acceptable through a simulation-based certification process that involves generating a synthetic wind record and using it as an input to a computer model of the turbine. Naturally, whether the simulation loads reflect the loads that the turbine would actually experience depends on the accuracy of the wind turbine model and, more importantly, on the accuracy of the method used to generate the synthetic wind record. The simulation methods that are commonly used for this purpose are spectral-based and produce Gaussian, stationary random fields. These methods prescribe a power spectral density (PSD) of the wind velocity, which fixes the magnitudes of the Fourier components, then assumes that the Fourier phase angles are independent and uniformly distributed. An inverse Fast Fourier Transform (IFFT) is then used to transform the wind velocity field to the time domain.</p><p>This thesis applies the concept of phase coherence---i.e., Fourier phase angles that are not independent---to the stochastic modeling and simulation of wind velocity fields. Using a large dataset available from the National Wind Technology Center (NWTC), a joint distribution is characterized for the mean wind speed <italic>U</italic>, turbulence &sigma;_u, Kaimal length scale <italic>L</italic>, and a metric for the degree of phase coherence in wind data, <italic>R</italic>. The correlations between these four parameters, the vertical height, and another phase coherence parameter are presented; only <italic>U</italic>, &sigma;_u, and <italic>L</italic> have any significant degree of correlation. The joint distribution is used to generate synthetic wind records, which are then compared with measured data that have the same parameter values. For data with low to medium coherence values, the synthetic records have a similar qualitative appearance to the data. For high levels of phase coherence, the records simulated with the proposed model were qualitatively different from records with the same parameter values due to the variation of the phase difference spread in the spectral domain. Lastly, the importance of correctly modeling phase coherence is demonstrated by using the data and the synthetic records as inputs to a single-degree-of-freedom (SDOF) oscillator and comparing the peak response statistics and damage equivalent loads (DELs).</p>Thesi

Disrupting Ovarian Cancer Metastatic Colonization: Insights from Metastasis Suppressor Studies

Ovarian cancer affects approximately 25,000 women in the United States each year and remains one of the most lethal female malignancies. A standard approach to therapy is surgical cytoreduction, after which the remaining microscopic residual disease is treated with chemotherapy. The vast majority of patients have disease recurrence, underscoring the crucial need for approaches to control the regrowth, or colonization, of tissues after local treatment. Improved therapies require mechanistic information about the process of metastatic colonization, the final step in metastasis, in which cancer cells undergo progressive growth at secondary sites. Studies of metastasis suppressors are providing insights into events controlling metastatic colonization. This paper reviews our laboratory's approach to the identification, characterization, and functional testing of the JNKK1/MKK4 metastasis suppressor in ovarian cancer metastatic colonization. Specifically, we demonstrate that interaction of ovarian caner cells with the omental microenvironment activates JNKK1/MKK4 resulting in decreased proliferation without affecting apoptosis. The potential role of the omental microenvironment, specifically milky spot structures, is also described. It is our goal to provide this work as a usable paradigm that will enable others to study metastasis suppressors in clinical and experimental ovarian cancer metastases

Histone Acetylation in the Nucleus Accumbens Shell Modulates Ethanol-Induced Locomotor Activity in DBA/2J Mice

A growing body of literature suggests that epigenetic mechanisms, including histone acetylation, may play key roles in drug abuse and the development of addiction. Experiments in the present study were designed to investigate the role of histone acetylation in ethanol-induced locomotor sensitization

IL-1 receptor signaling in the basolateral amygdala modulates binge-like ethanol consumption in male C57BL/6J mice

Proinflammatory cytokines have been implicated in alcohol-induced neurodegeneration, but the role of the neuroimmune system in alcohol related behaviors has only recently come to the forefront. Herein, the effects of binge-like drinking on IL-1β mRNA and immunoreactivity within the amygdala were measured following the “drinking in the dark” (DID) paradigm, a model of binge-like ethanol drinking in C57BL/6J mice. Moreover, the role of IL-1 receptor signaling in the amygdala on ethanol consumption was assessed. Results indicated that a history of binge-like ethanol drinking promoted a significant increase of IL-1β mRNA expression within the amygdala, and immunohistochemistry analyses revealed that the basolateral amygdala (BLA), but not central amygdala (CeA), exhibited significantly increased IL-1β immunoreactivity. Fluoro-Jade® C labeling indicated that multiple cycles of the DID paradigm were not sufficient to elicit neuronal death. Bilateral infusions of IL-1 receptor antagonist (IL-1Ra) reduced ethanol consumption when infused into the BLA but not the CeA. These observations were specific to ethanol drinking as the IL-1Ra did not alter either sucrose drinking or open-field locomotor activity. The current findings highlight a specific role for IL-1 receptor signaling in modulating binge-like ethanol consumption and indicate that proinflammatory cytokines can be induced prior to dependence or any evidence of neuronal cell death. These findings provide a framework in which to understand how neuroimmune adaptations may alter ethanol consumption and therein contributing to alcohol abuse

Assessment of the Effects of 6 Standard Rodent Diets on Binge-Like and Voluntary Ethanol Consumption in Male C57BL/6J Mice

In recent years much attention has been given to the lack of reproducibility in biomedical research, particularly in pre-clinical animal studies. This is a problem that also plagues the alcohol research field, particularly in consistent consumption in animal models of alcohol use disorders. One often overlooked factor that could affect reproducibility is the maintenance diet used in pre-clinical studies

Microhabitat use and spatial distribution in Picado’s Bromeliad Treefrog, Isthmohyla picadoi (Anura, Hylidae)

Isthmohyla picadoi is a Neotropical hylid frog found in upper humid montane forests of Costa Rica and Panama. The species is of particular interest because it continues to persist in an area in which the amphibian community has otherwise been decimated by the pathogenic fungus, Batrachochytrium dendrobatidis. Ground search, ladder climbing, and tree climbing techniques were used to locate 32 individuals; including adult males and females, juveniles, andmetamorphosing frogs. The majority of frogs were found in bromeliads, although some individuals were found on plants of the Euphorbiaceae, Musaceae, and Heliconiaceae families. Most frogs were found in larger bromeliads (45 cm or wider). There was a positive correlation between SUL and bromeliad width within the population but not within maturity classes (adult males, adult females, all adults, nonmetamorphosingjuveniles), suggesting that juvenile and adult frogs differ in bromeliad usage. Ranges of SUL and body weight in this particular population are much greater than those reported in previous species accounts

Repeated Cycles of Binge-Like Ethanol Drinking in Male C57BL/6J Mice Augments Subsequent Voluntary Ethanol Intake But Not Other Dependence-Like Phenotypes

Recently, procedures have been developed to model specific facets of human alcohol abuse disorders, including those that model excessive binge-like drinking (i.e., “drinking in the dark”, or DID procedures) and excessive dependence-like drinking (i.e., intermittent ethanol vapor exposure). Similar neuropeptide systems modulate excessive ethanol drinking stemming from both procedures, raising the possibility that both paradigms are actually modeling the same phenotypes and triggering the same central neuroplasticity. Therefore, the goal of the present project was to study the effects of a history of binge-like ethanol drinking, using DID procedures, on phenotypes that have previously been described with procedures to model dependence-like drinking