19 research outputs found
Natural product based anti-adhesion therapy : innovative prevention against bacterial infections
Proceeding volume: 13Peer reviewe
Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues
Berries of genus Vaccinium are rich in flavonoids and proanthocyanidins (PAs). We studied the PA composition and biosynthesis in bilberry (Vaccinium myrtillus L.) tissues and during fruit development. Soluble PAs, analyzed by UHPLC-MS/MS, were most abundant in stem and rhizome with the mean PA polymerization level varying between 4 and 6 in all tissues. Both A- and B-type PAs were present in all tissues. Procyanidin subunits were more common than prodelphinidin subunits in PAs. During fruit ripening, the amount of procyanidin subunits decreased while prodelphinidin subunits and F3'5'H expression increased, indicating a shift in biosynthesis toward the delphinidin branch of the flavonoid pathway. Epicatechin was the most abundant flavan-3-ol in all tissues. Expression of ANR and three isolated LAR genes, analyzed by qRT-PCR, showed connection to accumulation of PAs and flavan-3-ols biosynthesized from different flavonoid branches. Insoluble PAs accumulated during berry development, suggesting that PAs are not recycled after biosynthesis
Metabolic and molecular analysis of white mutant Vaccinium berries show down-regulation of MYBPA1-type R2R3 MYB regulatory factor
Main conclusion
MYBPA1-type R2R3 MYB transcription factor shows down-regulation in white mutant berries
of Vaccinium uliginosum deficient in anthocyanins but not proanthocyanidins suggesting a role in
the regulation of anthocyanin biosynthesis.
Berries of the genus Vaccinium are among the best natural sources of flavonoids. In this study, the
expression of structural and regulatory flavonoid biosynthetic genes and the accumulation of flavonoids
in white mutant and blue-colored wild-type bog bilberry (V. uliginosum) fruits were measured at
different stages of berry development. In contrast to high contents of anthocyanins in ripe blue-colored
berries, only traces were detected by HPLC-ESI-MS in ripe white mutant berries. However, similar
profile and high levels of flavonol glycosides and proanthocyanidins were quantified in both ripe white
and ripe wild-type berries. Analysis with qRT-PCR showed strong down-regulation of structural genes
chalcone synthase (VuCHS), dihydroflavonol 4-reductase (VuDFR) and anthocyanidin synthase
(VuANS) as well as MYBPA1-type transcription factor VuMYBPA1 in white berries during ripening
compared to wild-type berries. The profiles of transcript accumulation of chalcone isomerase (VuCHI),
anthocyanidin reductase (VuANR), leucoanthocyanidin reductase (VuLAR) and flavonoid 3′5′
hydroxylase (VuF3′5′H) were more similar between the white and the wild-type berries during fruit
development, while expression of UDP-glucose: flavonoid 3-O-glucosyltransferase (VuUFGT) showed
similar trend but fourfold lower level in white mutant. VuMYBPA1, the R2R3 MYB family member, is
a homologue of VmMYB2 of V. myrtillus and VcMYBPA1 of V. corymbosum and belongs to MYBPA1-
type MYB family which members are shown in some species to be related with proanthocyanidin
biosynthesis in fruits. Our results combined with earlier data of the role of VmMYB2 in white mutant
berries of V. myrtillus suggest that the regulation of anthocyanin biosynthesis in Vaccinium species
could differ from other species studied
Chemical composition and phenolic compound profile of mortiño (vaccinium floribundum kunth)
The phenolic compounds in mortiño (Vaccinium floribundum Kunth, family Ericaceae) from the páramos of Ecuador were studied by LC-DAD−MS/MS for the first time. (−)-Epicatechin, one dimer A and one trimer A were found at a total concentration of 18 mg/100 g FW. Of the flavonol glycosides (38 mg/100 g FW), quercetin and myricetin were found as -3-O-hexosides, -3-O-pentosides and -3-O-deoxyhexosides. Chlorogenic and neochlorogenic acids together with caffeic/ferulic acid derivatives were found as predominant components among the hydroxycinnamic acids in the berry. Anthocyanins, including cyanidin and delphinidin derivatives, were the major phenolic compound class quantified (345 mg cyanidin-3-O-glucoside/100 g FW)
Antimicrobial activity of different F
The antimicrobial activity and phenolic compounds of five Finnish honey products against important human pathogens Streptococcus pneumoniae, S. pyogenes, Staphylococcus aureus, and methicillin-resistant S. aureus were analyzed. Microbroth dilution method and HPLC-DAD were used in antimicrobial testing and phenolic compound determination, respectively. Significant antimicrobial activity (p < 0.01) against all the tested pathogens was found from willow herb (Epilobium angustifolium), heather (Calluna vulgaris), and buckwheat (Fagopyrum esculentum) honeys. This is the first report on antimicrobial activity of Finnish monofloral honeys against streptococcal and staphylococcal bacteria. To our knowledge this is also the first report on the antimicrobial effect of honey against S. pneumoniae