Clinical impact of the disposable ventouse iCup® versus a metallic vacuum cup: a multicenter randomized controlled trial

Experimental Protocole. (DOCX 55 kb

Nano-surgery at the leukocyte–endothelial docking site

The endothelium has an important role in controlling the extravasation of leukocytes from blood to tissues. Endothelial permeability for leukocytes is influenced by transmembrane proteins that control inter-endothelial adhesion, as well as steps of the leukocyte transmigration process. In a cascade consisting of leukocyte rolling, adhesion, firm adhesion, and diapedesis, a new step was recently introduced, the formation of a docking structure or “transmigratory cup.” Both terms describe a structure formed by endothelial pseudopods embracing the leukocyte. It has been found associated with both para- and transcellular diapedesis. The aim of this study was to characterize the leukocyte–endothelial contact area in terms of morphology and cell mechanics to investigate how the endothelial cytoskeleton reorganizes to engulf the leukocyte. We used atomic force microscopy (AFM) to selectively remove the leukocyte and then analyze the underlying cell at this specific spot. Firmly attached leukocytes could be removed by AFM nanomanipulation. In few cases, this exposed 8–12 μm wide and 1 μm deep footprints, representing the cup-like docking structure. Some of them were located near endothelial cell junctions. The interaction area did not exhibit significant alterations neither morphologically nor mechanically as compared to the surrounding cell surface. In conclusion, the endothelial invagination is formed without a net depolymerization of f-actin, as endothelial softening at the site of adhesion does not seem to be involved. Moreover, there were no cases of phagocytotic engulfment, but instead the formation of a transmigratory channel could be observed

Local density information obtained by means of the Background Oriented Schlieren (BOS) method

The fairly new measurement system BOS is providing promising results by allowing the visualisation of qualitative density gradients as presented by Richard et al (2000). This paper shows that it is possible to measure local density information by means of BOS for the specific application of a 2D jet. An experiment is described to present the feasibility of BOS to measure local density information. A slit nozzle blowing an air / helium mixture was investigated by BOS and Catharometry. Comparing the obtained results and taking the Catharometry measurement as the reference, the accuracy of the density information provided by BOS can be evaluated: The small difference between the results of the two measurement techniques confirms the applicability and accuracy of BOS for local density measurements in 2D density gradient investigations

Développements récents de la Technique de l’Arc-en-ciel appliquée aux gouttes avec évaporation

status: publishe

Validation experimentale de simulations de thermometrie par la methode d’arc-en-ciel global

status: publishe

Global rainbow thermometry validation in liquid-liquid suspension and applications to water spray

status: publishe

Temperature gradient measurements in combustion droplets by standard rainbow thermometry

status: publishe

Global rainbow thermometry validation in liquid-liquid suspension

status: publishe

Mesure de la température d’un spray d’eau chaude par la méthode globale par arc-en-ciel : effet du gradient de température dans les gouttes

status: publishe

Droplet internal recirculation measurement by micro-PIV

info:eu-repo/semantics/nonPublishe