Neonatal Calf Care

Calf mortality is important economically in dairy and beef herds. Calf losses substantially reduce the returns from cattle raising. The economic losses are those derived from the loss of the calf, planning and labor already invested by the herdsman, and veterinary expenses from obstetric and post-natal care. Indirect losses are infertility, chronic disease, and impaired development of the calf

Infertility in the Mare

Infertility in the mare results in a significant loss of dollars in the horse industry every year. It is defined as the absence of the ability to conceive. There are many causes of infertility that are recognized, including infectious, inflammatory, a faulty uterine immune system, trauma and scarring, hormonal, twinning, neoplasia, and congenital abnormalities

Exogenous Control of the Estrous Cycle in the Mare

The control of estrus, and more importantly ovulation, may be desirable in a breeding program to service an animal at a predetermined time, to synchronize ovulation in a group of mares, or to spread ovulations over a prolonged period of time according to the various needs of managing the breeding animals. Ideally, the goal would be to control ovulation with enough precision that breeding could be done without the necessity of estrus detection

Pelvic Measurements: Applications in Beef Cattle Practice Today

Dystocia (difficult calving) is a major cause of loss for beef cattle producers. It results in an increased incidence of calf mortality at or near birth, increased cow mortality, and higher veterinary and labor costs. In one study, calf loss within 24 hours of birth was 4% when heifers gave birth unassisted, 16% when calving difficulty ensued. Of the calves that died at or near calving, 57% of these were attributed to dystocia.2Another study revealed that heifers calving unassisted weaned 70% of their calves; heifers needing assistance calving weaned 59%. Dystocia also results in delayed return to estrus and decreased conception rate in heifers and cows subsequent to their calving difficulty. Pregnancy rates following first calving in one study were 85% for heifers calving unassisted and 69% for heifers that had dystocia. Subsequent weaning rates for heifers that underwent dystocia in their first calving were !4% lower than for their non-dystocia counterparts

Essential Role for Cathepsin S in MHC Class II–Associated Invariant Chain Processing and Peptide Loading

AbstractDestruction of Ii by proteolysis is required for MHC class II molecules to bind antigenic peptides, and for transport of the resulting complexes to the cell surface. The cysteine protease cathepsin S is highly expressed in spleen, lymphocytes, monocytes, and other class II–positive cells, and is inducible with interferon-γ. Specific inhibition of cathepsin S in B lymphoblastoid cells prevented complete proteolysis of Ii, resulting in accumulation of a class II–associated 13 kDa Ii fragment in vivo. Consequently, the formation of SDS-stable complexes was markedly reduced. Purified cathepsin S, but not cathepsin B, H, or D, specifically digested Ii from αβIi trimers, generating αβ–CLIP complexes capable of binding exogenously added peptide in vitro. Thus, cathepsin S is essential in B cells for effective Ii proteolysis necessary to render class II molecules competent for binding peptides

Effects of tofacitinib and other DMARDs on lipid profiles in rheumatoid arthritis: implications for the rheumatologist

Cardiovascular (CV) morbidity and mortality are increased in patients with active, untreated rheumatoid arthritis (RA), despite lower levels of total and low-density lipoprotein cholesterol reported in individuals with active RA compared with those without RA. Alterations in non-traditional lipid assessments, such as high-density lipoprotein (HDL) function and HDL-associated proteins, have been described in patients with active RA, including elevated HDL-associated serum amyloid A and decreased paraoxonase-1 activity. We review changes in both traditional lipoprotein concentrations and non-traditional lipoprotein assessments in multiple studies of treatment with disease-modifying antirheumatic drugs (DMARDs), including non-biologic and biologic DMARDs and tofacitinib. In addition, data from a recently published clinical trial with tofacitinib that describe a potential mechanism for suppression of cholesterol levels in active RA patients are reviewed. Finally, CV event data from various studies of DMARDs are presented, and the current management of RA patients with regard to the CV risk is reviewed

User requirements for monitoring the evolution of stratospheric ozone at high vertical resolution (‘Operoz’: Operational ozone observations using limb geometry)

The purpose of the Operoz study has been threefold: (i) To establish the user requirements for an operational mission targeting ozone profiles at high vertical resolution, (ii) To identify the observational gaps with respect to these user requirements taking into account planned operational missions and observational ground networks, and (iii) To perform a reality check on the observational requirements based on proven concepts and present-day knowledge of potentially available measurement techniques and to identify options for a small to medium size satellite mission

Anxiety sensitivity, its stability and longitudinal association with severity of anxiety symptoms

Anxiety sensitivity is associated with the onset of panic attacks, anxiety, and other common mental disorders. Anxiety sensitivity is usually seen as a relative stable trait. However, previous studies were inconclusive regarding the longitudinal stability of anxiety sensitivity and differed in study designs and outcomes. The current study examines the stability of anxiety sensitivity over time and its longitudinal associations with severity of anxiety symptoms. Participants from the Netherlands Study of Depression and Anxiety with and without an anxiety, depressive, or comorbid anxiety-depressive disorder diagnosis were included (N = 2052). Stability in anxiety sensitivity over two year follow-up and the longitudinal association between the change in anxiety sensitivity and change in severity of anxiety symptoms were tested. Results indicated that two-year stability of anxiety sensitivity was high (r = 0.72), yet this test-retest estimate leaves room for changes in anxiety sensitivity in some individuals as well. Change in anxiety sensitivity was positively associated with change in severity of anxiety symptoms (B = 0.64 in univariable analysis and B = 0.52 in multivariable analysis). The longitudinal association of anxiety sensitivity with severity of anxiety symptoms indicates that targeting anxiety sensitivity may be of additional benefit in clinical practice

Mirc11 Disrupts Inflammatory but Not Cytotoxic Responses of NK Cells

Natural killer (NK) cells generate proinflammatory cytokines that are required to contain infections and tumor growth. However, the posttranscriptional mechanisms that regulate NK cell functions are not fully understood. Here, we define the role of the microRNA cluster known as Mirc11 (which includes miRNA-23a, miRNA-24a, and miRNA-27a) in NK cell–mediated proinflammatory responses. Absence of Mirc11 did not alter the development or the antitumor cytotoxicity of NK cells. However, loss of Mirc11 reduced generation of proinflammatory factors in vitro and interferon-γ–dependent clearance of Listeria monocytogenes or B16F10 melanoma in vivo by NK cells. These functional changes resulted from Mirc11 silencing ubiquitin modifiers A20, Cbl-b, and Itch, allowing TRAF6-dependent activation of NF-κB and AP-1. Lack of Mirc11 caused increased translation of A20, Cbl-b, and Itch proteins, resulting in deubiquitylation of scaffolding K63 and addition of degradative K48 moieties on TRAF6. Collectively, our results describe a function of Mirc11 that regulates generation of proinflammatory cytokines from effector lymphocytes