753 research outputs found
Mechanically induced helix-coil transition in biopolymer networks
The quasi-equilibrium evolution of the helical fraction occurring in a
biopolymer network (gelatin gel) under an applied stress has been investigated
by observing modulation in its optical activity. Its variation with the imposed
chain extension is distinctly non-monotonic and corresponds to the transition
of initially coiled strands to induced left-handed helices. The experimental
results are in qualitative agreement with theoretical predictions of helices
induced on chain extension. This new effect of mechanically stimulated
helix-coil transition has been studied further as a function of the elastic
properties of the polymer network: crosslink density and network aging
Reversible stretching of homopolymers and random heteropolymers
We have analyzed the equilibrium response of chain molecules to stretching.
For a homogeneous sequence of monomers, the induced transition from compact
globule to extended coil below the -temperature is predicted to be
sharp. For random sequences, however, the transition may be smoothed by a
prevalence of necklace-like structures, in which globular regions and coil
regions coexist in a single chain. As we show in the context of a random
copolymer, preferential solvation of one monomer type lends stability to such
structures. The range of stretching forces over which necklaces are stable is
sensitive to chain length as well as sequence statistics.Comment: 14 pages, 4 figure
Resistance training concomitant to radiotherapy of spinal bone metastases – survival and prognostic factors of a randomized trial
There and (slowly) back again: Entropy-driven hysteresis in a model of DNA overstretching
When pulled along its axis, double-stranded DNA elongates abruptly at a force
of about 65 pN. Two physical pictures have been developed to describe this
overstretched state. The first proposes that strong forces induce a phase
transition to a molten state consisting of unhybridized single strands. The
second picture instead introduces an elongated hybridized phase, called S-DNA,
structurally and thermodynamically distinct from standard B-DNA. Little
thermodynamic evidence exists to discriminate directly between these competing
pictures. Here we show that within a microscopic model of DNA we can
distinguish between the dynamics associated with each. In experiment,
considerable hysteresis in a cycle of stretching and shortening develops as
temperature is increased. Since there are few possible causes of hysteresis in
a system whose extent is appreciable in only one dimension, such behavior
offers a discriminating test of the two pictures of overstretching. Most
experiments are performed upon nicked DNA, permitting the detachment
(`unpeeling') of strands. We show that the long-wavelength progression of the
unpeeled front generates hysteresis, the character of which agrees with
experiment only if we assume the existence of S-DNA. We also show that internal
melting (distinct from unpeeling) can generate hysteresis, the degree of which
is strongly dependent upon the nonextensive loop entropy of single-stranded
DNA.Comment: 18 pages, 10 figure
Extracting Structural Information of a Heteropolymer from Force-Extension Curves
We present a theory for the reverse analysis on the sequence information of a
single H/P two-letter random hetero-polymer (RHP) from its force-extension(f-z)
curves during quasi static stretching. Upon stretching of a self-assembled RHP,
it undergoes several structural transitions. The typical elastic response of a
hetero-polymeric globule is a set of overlapping saw-tooth patterns. With
consideration of the height and the position of the overlapping saw-tooth
shape, we analyze the possibility of extracting the binding energies of the
internal domains and the corresponding block sizes of the contributing
conformations.Comment: 5 figures 7 page
Theory of biopolymer stretching at high forces
We provide a unified theory for the high force elasticity of biopolymers
solely in terms of the persistence length, , and the monomer spacing,
. When the force f>\fh \sim k_BT\xi_p/a^2 the biopolymers behave as Freely
Jointed Chains (FJCs) while in the range \fl \sim k_BT/\xi_p < f < \fh the
Worm-like Chain (WLC) is a better model. We show that can be estimated
from the force extension curve (FEC) at the extension
(normalized by the contour length of the biopolymer). After validating the
theory using simulations, we provide a quantitative analysis of the FECs for a
diverse set of biopolymers (dsDNA, ssRNA, ssDNA, polysaccharides, and
unstructured PEVK domain of titin) for . The success of a specific
polymer model (FJC or WLC) to describe the FEC of a given biopolymer is
naturally explained by the theory. Only by probing the response of biopolymers
over a wide range of forces can the -dependent elasticity be fully
described.Comment: 20 pages, 4 figure
A Quantitative Theory of Mechanical Unfolding of a Homopolymer Globule
We propose the quantitative mean-field theory of mechanical unfolding of a
globule formed by long flexible homopolymer chain collapsed in poor solvent and
subjected to extensional deformation. We demonstrate that depending on the
degree of polymerization and solvent quality (quantified by the Flory-Huggins
parameter) the mechanical unfolding of the collapsed chain may either
occur continuously (by passing a sequence of uniformly elongated
configurations) or involves intra-molecular micro-phase coexistence of a
collapsed and a stretched segment followed by an abrupt unraveling transition.
The force-extension curves are obtained and quantitatively compared to our
recent results of numerical self-consistent field (SCF) simulations. The phase
diagrams for extended homopolymer chains in poor solvent comprising one- and
two-phase regions are calculated for different chain length or/and solvent
quality.Comment: 24 pages, 18 figure
Rupture of multiple parallel molecular bonds under dynamic loading
Biological adhesion often involves several pairs of specific receptor-ligand
molecules. Using rate equations, we study theoretically the rupture of such
multiple parallel bonds under dynamic loading assisted by thermal activation.
For a simple generic type of cooperativity, both the rupture time and force
exhibit several different scaling regimes. The dependence of the rupture force
on the number of bonds is predicted to be either linear, like a square root or
logarithmic.Comment: 8 pages, 2 figure
Stretched Polymers in a Poor Solvent
Stretched polymers with attractive interaction are studied in two and three
dimensions. They are described by biased self-avoiding random walks with
nearest neighbour attraction. The bias corresponds to opposite forces applied
to the first and last monomers. We show that both in and a phase
transition occurs as this force is increased beyond a critical value, where the
polymer changes from a collapsed globule to a stretched configuration. This
transition is second order in and first order in . For we
predict the transition point quantitatively from properties of the unstretched
polymer. This is not possible in , but even there we can estimate the
transition point precisely, and we can study the scaling at temperatures
slightly below the collapse temperature of the unstretched polymer. We find
very large finite size corrections which would make very difficult the estimate
of the transition point from straightforward simulations.Comment: 10 pages, 16 figure
Differences in trauma history and psychopathology between PTSD patients with and without co-occurring dissociative disorders
Wabnitz P, Gast U, Catani C. Differences in trauma history and psychopathology between PTSD patients with and without co-occurring dissociative disorders. European Journal of Psychotraumatology. 2013;2013(4): 21452.Background: The interplay between different types of potentially traumatizing events, posttraumatic symptoms, and the pathogenesis of PTSD or major dissociative disorders (DD) has been extensively studied during the last decade. However, the phenomenology and nosological classification of posttraumatic disorders is currently under debate. The current study was conducted to investigate differences between PTSD patients with and without co-occurring major DD with regard to general psychopathology, trauma history, and trauma-specific symptoms.
Methods: Twenty-four inpatients were administered the Clinician-Administered PTSD Scale for DSM-IV (CAPS) and the Mini-Structured Clinical Interview for DSM-IV Dissociative Disorders (MINI-SKID-D) to assess DD and PTSD. Additionally, participants completed questionnaires to assess general psychopathology and health status.
Results: Symptom profiles and axis I comorbidity were similar in all patients. Traumatic experiences did not differ between the two groups, with both reporting high levels of childhood trauma. Only trauma-specific avoidance behavior and dissociative symptoms differed between groups.
Conclusion: Results support the view that PTSD and DD are affiliated disorders that could be classified within the same diagnostic category. Our results accord with a typological model of dissociation in which profound forms of dissociation are specific to DD and are accompanied with higher levels of trauma-specific avoidance in DD patients
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