Port Stanley Magnetic Observatory installation report 6 - 10 February 2003

This report describes the modifications made to the Port Stanley Geomagnetic Observatory during a visit to the Falkland Islands in February 2003. Since the report is intended as a technical reference for observatory operation, the instruments, computing equipment, software and cabling have been described in detail. The modifications were undertaken to ensure the continued supply of high quality geomagnetic data from this observatory, and to this end, there are some further recommendations for future visits listed in the final section of the report

Multiscale Analysis of Delamination of Carbon Fiber-Epoxy Laminates with Carbon Nanotubes

A multi-scale analysis is presented to parametrically describe the Mode I delamination of a carbon fiber/epoxy laminate. In the midplane of the laminate, carbon nanotubes are included for the purposes of selectively enhancing the fracture toughness of the laminate. To analyze carbon fiber epoxy carbon nanotube laminate, the multi-scale methodology presented here links a series of parameterizations taken at various length scales ranging from the atomistic through the micromechanical to the structural level. At the atomistic scale molecular dynamics simulations are performed in conjunction with an equivalent continuum approach to develop constitutive properties for representative volume elements of the molecular structure of components of the laminate. The molecular-level constitutive results are then used in the Mori-Tanaka micromechanics to develop bulk properties for the epoxy-carbon nanotube matrix system. In order to demonstrate a possible application of this multi-scale methodology, a double cantilever beam specimen is modeled. An existing analysis is employed which uses discrete springs to model the fiber bridging affect during delamination propagation. In the absence of empirical data or a damage mechanics model describing the effect of CNTs on fracture toughness, several tractions laws are postulated, linking CNT volume fraction to fiber bridging in a DCB specimen. Results from this demonstration are presented in terms of DCB specimen load-displacement responses

A Simple, Quick, and Precise Procedure for the Determination of Water in Organic Solvents

A procedure for the UV/VIS-spectroscopic determination of water by the use of a solvatochromic pyridiniumphenolate betaine is given. The water content of organic solvents is calculated by a two parameter equation from λmax of the dye. A typical, detection limit is of the order of 1 mg in 1 ml solvent for routine spectrometers. The parameters for the determination of water are given for a number of commonly used solvents

Buckling of Carbon Nanotube-Reinforced Polymer Laminated Composite Materials Subjected to Axial Compression and Shear Loadings

A multi-scale method to predict the stiffness and stability properties of carbon nanotube-reinforced laminates has been developed. This method is used in the prediction of the buckling behavior of laminated carbon nanotube-polyethylene composites formed by stacking layers of carbon nanotube-reinforced polymer with the nanotube alignment axes of each layer oriented in different directions. Linking of intrinsic, nanoscale-material definitions to finite scale-structural properties is achieved via a hierarchical approach in which the elastic properties of the reinforced layers are predicted by an equivalent continuum modeling technique. Solutions for infinitely long symmetrically laminated nanotube-reinforced laminates with simply-supported or clamped edges subjected to axial compression and shear loadings are presented. The study focuses on the influence of nanotube volume fraction, length, orientation, and functionalization on finite-scale laminate response. Results indicate that for the selected laminate configurations considered in this study, angle-ply laminates composed of aligned, non-functionalized carbon nanotube-reinforced lamina exhibit the greatest buckling resistance with 1% nanotube volume fraction of 450 nm uniformly-distributed carbon nanotubes. In addition, hybrid laminates were considered by varying either the volume fraction or nanotube length through-the-thickness of a quasi-isotropic laminate. The ratio of buckling load-to-nanotube weight percent for the hybrid laminates considered indicate the potential for increasing the buckling efficiency of nanotube-reinforced laminates by optimizing nanotube size and proportion with respect to laminate configuration

Learn to Fly Test Setup and Concept of Operations

The NASA Learn-to-Fly (L2F) project recently completed a series of flight demonstrations of its learning algorithm for flight control at Fort A. P. Hill in Virginia. This paper discusses the test setup and concept of operations (ConOps) used by the L2F team. Unmanned airframe demonstrators for testing the research algorithms included a modified commercial off-the-shelf subscale powered airplane, plus four gliders two of which had an unconventional configuration and were fabricated using a rapid prototyping technique. Avionics system similarities and differences between the test aircraft are described, as well as ground testing in preparation for flight. The ConOps discussion includes the development of a tethered helium balloon drop launch technique for the glider demonstrators. This launch method was chosen for its potential to be inexpensive and allow for rapid turn-around for multiple glider launches but it also presented challenges, such as balloon tether avoidance, high angle of attack, low dynamic pressure initial conditions, and susceptibility to winds. A remotely piloted approach employing high-end hobbyist radio controlled (R/C) hardware was used for the powered demonstrator. This approach accommodated the interaction between the R/C flight system and the research flight control computer, engaging the L2F algorithm at varying initial conditions and artificially reducing the aircraft stability to stress the algorithm

Compression-Loaded Composite Panels With Elastic Edge Restraints and Initial Prestress

A parametric study of the effects of test-fixture-induced initial prestress and elastic edge restraints on the prebuckling and buckling responses of a compression-loaded, quasi-isotropic curved panel is presented. The numerical results were obtained by using a geometrically nonlinear finite element analysis code with high-fidelity models. The results presented show that a wide range of prebuckling and buckling behavior can be obtained by varying parameters that represent circumferential loaded-edge restraint and rotational unloaded-edge restraint provided by a test fixture and that represent the mismatch in specimen and test-fixture radii of curvature. For a certain range of parameters, the panels exhibit substantial nonlinear prebuckling deformations that yield buckling loads nearly twice the corresponding buckling load predicted by a traditional linear bifurcation buckling analysis for shallow curved panels. In contrast, the results show another range of parameters exist for which the nonlinear prebuckling deformations either do not exist or are relatively benign, and the panels exhibit buckling loads that are nearly equal to the corresponding linear bifurcation buckling load. Overall, the results should be of particular interest to scientists, engineers, and designers involved in simulating flight-hardware boundary conditions in structural verification and certification tests, involved in validating structural analysis tools, and interested in tailoring buckling performance

Infrared identification of hard X-ray sources in the Galaxy

The nature of the low- to intermediate-luminosity (LX ∼ 1032–34 erg s−1) source population revealed in hard band (2–10 keV) X-ray surveys of the Galactic plane is poorly understood. To overcome such problem, we cross-correlated the XMM–Newton 3XMM-DR4 survey with the infrared Two Micron All Sky Survey and Galactic Legacy Infrared Mid-Plane Survey Extraordinaire catalogues. We identified reliable X-ray–infrared associations for 690 sources. We selected 173 sources having hard X-ray spectra, typical of hard X-ray high-mass stars (kT > 5 keV), and 517 sources having soft X-ray spectra, typical of active coronae. About 18 per cent of the soft sources are classified in the literature: ∼91 per cent as stars, with a minor fraction of Wolf–Rayet (WR) stars. Roughly 15 per cent of the hard sources are classified in the literature: ∼68 per cent as high-mass X-ray stars single or in binary systems (WR, Be and high-mass X-ray binaries – HMXBs), with a small fraction of G and B stars. We carried out infrared spectroscopic pilot observations at the William Herschel Telescope for five hard X-ray sources. Three of them are high-mass stars with spectral types WN7-8h, Ofpe/WN9 and Be, and LX ∼ 1032–1033erg s−1. One source is a colliding-wind binary, while another source is a colliding-wind binary or a supergiant fast X-ray transient in quiescence. The Be star is a likely γ-Cas system. The nature of the other two X-ray sources is uncertain. The distribution of hard X-ray sources in the parameter space made of X-ray hardness ratio, infrared colours and X-ray-to-infrared flux ratio suggests that many of the unidentified sources are new γ-Cas analogues, WRs and low LX HMXBs. However, the nature of the X-ray population with Ks ≥ 11 and average X-ray-to-infrared flux ratio remains unconstrained.We acknowledge financial support from the ARCHES project (7th Framework of the European Union, no. 313146). FJC acknowledges financial support from the Spanish Ministerio de Economía y Competitividad under project AYA2012-31447.Peer Reviewe

Micro-Environment Causes Reversible Changes in DNA Methylation and mRNA Expression Profiles in Patient-Derived Glioma Stem Cells

In vitro and in vivo models are widely used in cancer research. Characterizing the similarities and differences between a patient\u27s tumor and corresponding in vitro and in vivo models is important for understanding the potential clinical relevance of experimental data generated with these models. Towards this aim, we analyzed the genomic aberrations, DNA methylation and transcriptome profiles of five parental tumors and their matched in vitro isolated glioma stem cell (GSC) lines and xenografts generated from these same GSCs using high-resolution platforms. We observed that the methylation and transcriptome profiles of in vitro GSCs were significantly different from their corresponding xenografts, which were actually more similar to their original parental tumors. This points to the potentially critical role of the brain microenvironment in influencing methylation and transcriptional patterns of GSCs. Consistent with this possibility, ex vivo cultured GSCs isolated from xenografts showed a tendency to return to their initial in vitro states even after a short time in culture, supporting a rapid dynamic adaptation to the in vitro microenvironment. These results show that methylation and transcriptome profiles are highly dependent on the microenvironment and growth in orthotopic sites partially reverse the changes caused by in vitro culturing

Differential expression of prognostic proteomic markers in primary tumour, venous tumour thrombus and metastatic renal cell cancer tissue and correlation with patient outcome.

Renal cell carcinoma (RCC) is the most deadly of urological malignancies. Metastatic disease affects one third of patients at diagnosis with a further third developing metastatic disease after extirpative surgery. Heterogeneity in the clinical course ensures predicting metastasis is notoriously difficult, despite the routine use of prognostic clinico-pathological parameters in risk stratification. With greater understanding of pathways involved in disease pathogenesis, a number of biomarkers have been shown to have prognostic significance, including Ki67, p53, vascular endothelial growth factor receptor 1 (VEGFR1) and ligand D (VEGFD), SNAIL and SLUG. Previous pathway analysis has been from study of the primary tumour, with little attention to the metastatic tumours which are the focus of targeted molecular therapies. As such, in this study a tissue microarray from 177 patients with primary renal tumour, renal vein tumour thrombus and/or RCC metastasis has been created and used with Automated Quantitative Analysis (AQUA) of immunofluorescence to study the prognostic significance of these markers in locally advanced and metastatic disease. Furthermore, this has allowed assessment of differential protein expression between the primary tumours, renal vein tumour thrombi and metastases. The results demonstrate that clinico-pathological parameters remain the most significant predictors of cancer specific survival; however, high VEGFR1 or VEGFD can predict poor cancer specific survival on univariate analysis for locally advanced and metastatic disease. There was significantly greater expression of Ki67, p53, VEGFR1, SLUG and SNAIL in the metastases compared with the primary tumours and renal vein tumour thrombi. With the exception of p53, these differences in protein expression have not been shown previously in RCC. This confirms the importance of proliferation, angiogenesis and epithelial to mesenchymal transition in the pathogenesis and metastasis of RCC. Importantly, this work highlights the need for further pathway analysis of metastatic tumours for overcoming drug resistance and developing new therapies