El rango de entonación microtónica en el canto kakataibo. Una aproximación fonética y musicológica a no bana ‘iti

En el presente artículo proponemos un análisis fonético y musicológico del canto tradicional no bana ‘iti de los kakataibo, grupo de la familia lingüística pano de la Amazonía peruana, con el fin de contrastar los resultados con lo dicho por varios autores sobre el microtonalismo como característica de dicho canto. Con base en el análisis fonético y musical del mismo, a diferencia de estos, que proponen la existencia de variaciones microtónicas y una melodía compuesta por dos o tres notas, en el presente estudio consideramos que el canto no bana ‘iti se compone de dos notas fluctuantes: una primera más alta (H), que recae en la primera sílaba, y una segunda más grave (L), que está asociada al resto de la línea melódica. Por último, estudiaremos la interacción de estas dos notas fluctuantes con el metro kakataibo y aplicaremos esta propuesta al canto ño xakwati como ejemplo para futuros análisis

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures

For the production of recombinant protein therapeutics in mammalian cells, a high rate of gene expression is desired and hence strong viral-derived promoters are commonly used. However, they usually induce cellular stress and can be susceptible to epigenetic silencing. Endogenous promoters, which coordinates their activity with cellular and bioprocess dynamics while at the same time they maintain high expression levels, may help to avoid such drawbacks. In this work, new endogenous promoters were discovered based on high expression levels in RNA-seq data of CHO-K1 cells cultured in high density. The promoters of Actb, Ctsz, Hmox1, Hspa5, Vim and Rps18 genes were selected for generating new expression vectors for the production of recombinant proteins in mammalian cells. The in silico-derived promoter regions were experimentally verified and the majority showed transcriptional activity comparable or higher than CMV. Also, stable expression following a reduction of culture temperature was investigated. The characterized endogenous promoters (excluding Rps18) constitute a promising alternative to CMV promoter due to their high strength, long-term expression stability and integration into the regulatory network of the host cell. These promoters may also comprise an initial panel for designing cell engineering strategies and synthetic promoters, as well as for industrial cell line development.Fil: Tossolini, Ileana del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Gugliotta, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: López Díaz, Fernando. Salk Institute for Biological Studies; Estados UnidosFil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentin

El principio de igualdad de trato en las Directivas comunitarias, en materia de Seguridad Social

Proyecto de investigación de la Dirección General de Ciencia y Tecnología del Ministerio de Educación y Cultura, Ref. P. B. 94-1 051-C02-01 . REVIST

Stability of a cyanidin-3-O-glucoside extract obtained from Arbutus unedo L. and incorporation into wafers for colouring purposes

An extract from Arbutus unedo fruits, rich in anthocyanins, was studied as a natural colorant with bioactive properties (antioxidant, antimicrobial and cytotoxic). The aqueous stability of the extract was monitored using the anthocyanins’ content as response (determined by HPLC-DAD) in function of time, temperature and pH. Aided by mechanistic/phenomenological models, the conditions that favours the stabilization of the extract were provided, highlighting the suitability of the colorant for pastry/bakery products. As a case study, the extract was incorporated into wafers and the changes on the nutritional profile, free sugars, fatty acids and antioxidant properties were monitored during 6 days of storage. The results provide information for: i) potential application of the rich extract in anthocyanins for producing a natural colorant with bioactive properties; and ii) shelf-life predictions. The extract incorporation did not cause changes in the nutritional components of wafers but added colorant and antioxidant properties.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), L. Barros contract and C. Caleja (SFRH/BD/93007/2013) grant. This work is funded by the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project NORTE- 01-0145-FEDER-023289: DeCodE and project Mobilizador Norte-01- 0247-FEDER-024479: ValorNatural®. The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E. To Xunta de Galicia for financial support for the post-doctoral researcher of M.A. Prieto.info:eu-repo/semantics/publishedVersio

Evaluación de la calidad ambiental de ecosistemas acuáticos en el entorno del Parque Nacional de Doñana

Resumen de la comunicación presentada al XX Congreso de la Sociedad Española de Mutagénesis Ambiental – Córdoba 201

Direccionamiento Estratégico para la Ferreteria Distribuciones RIBER SALAMANCA, Municipio de Acacias - Meta ./

104 hFormular el direccionamiento estratégico para la ferretería DISTRIBUCIONES RIBER SALAMANCA, aplicando matrices estratégicas adecuadas que permitan establecer directivas claras. • Definir estrategias a partir de la aplicación de la matriz de evaluación de factores internos y externos. • Identificar enfoques de liderazgo y motivación empresarial para la administración estratégica de la ferretería DISTRIBUCIONES RIBER SALAMANCA. • Elaborar los principios corporativos de la organización y sugerir reestructurar el organigrama acorde con las necesidades actuales del negocio. • Formular indicadores para medir la eficacia, eficiencia y efectividad de las actividades programadas. • Formular estrategias mediante la aplicación de la matriz PEYEA y la matriz MCPE, que permite ser más eficaz en los procesos.Resultado para Obtener el Título de Profesional en Administración Financiera, Tesis (Administrador Financiero) Universidad de los Llanos. Facultad de Ciencias Humanas. Programa de Administración financiera. Gerencia de Recursos Humanos, 2015PregradoEspecializaciones en Finanza

Cognitive reserve is associated with the functional organization of brain networks in healthy aging: a MEG study

The proportion of elderly people in the population has increased rapidly in the last century and consequently "healthy aging" is expected to become a critical area of research in neuroscience. Evidence reveals how healthy aging depends on three main behavioral factors: social lifestyle, cognitive activity and physical activity. In this study, we focused on the role of cognitive activity, concentrating specifically on educational and occupational attainment factors, which were considered two of the main pillars of cognitive reserve. 21 subjects with similar rates of social lifestyle, physical and cognitive activity were selected from a sample of 55 healthy adults. These subjects were divided into two groups according to their level of cognitive reserve; one group comprised subjects with high cognitive reserve (9 members) and the other contained those with low cognitive reserve (12 members). To evaluate the cortical brain connectivity network, all participants were recorded by Magnetoencephalography (MEG) while they performed a memory task (modified version of the Sternberg¿s Task). We then applied two algorithms (Phase Locking Value & Phase-Lag Index) to study the dynamics of functional connectivity. In response to the same task, the subjects with lower cognitive reserve presented higher functional connectivity than those with higher cognitive reserve. These results may indicate that participants with low cognitive reserve needed a greater 'effort' than those with high cognitive reserve to achieve the same level of cognitive performance. Therefore, we conclude that cognitive reserve contributes to the modulation of the functional connectivity patterns of the aging brain

Fiber-Optic Pyrometer with Optically Powered Switch for Temperature Mesurements

We report the experimental results on a new infrared fiber-optic pyrometer for very localized and high-speed temperature measurements ranging from 170 to 530 degrees C using low-noise photodetectors and high-gain transimpedance amplifiers with a single gain mode in the whole temperature range. We also report a shutter based on an optical fiber switch which is optically powered to provide a reference signal in an optical fiber pyrometer measuring from 200 to 550 degrees C. The tests show the potential of remotely powering via optical means a 300 mW power-hungry optical switch at a distance of 100 m, avoiding any electromagnetic interference close to the measuring point.This work was supported by the Spanish Ministry of Economy and Competitiveness and FEDER program under grants TEC2015-63826-C3-2-R and by Comunidad de Madrid under grant S2013/MIT-2790

Synchronization during an internally directed cognitive state in healthy aging and mild cognitive impairment: a MEG study

Mild Cognitive Impairment (MCI) is a stage between healthy aging and dementia. Functional connectivity is widely used to study the brain activity during resting state or cognitive tasks. We aimed to determine the functional connectivity changes required to deal with an Internally Directed Cognitive State (IDICS) in healthy aging and MCI. This task differs from the most commonly employed in Magnetoencephalograpy (MEG)/ Electroencephalography (EEG), since inhibition from external stimuli is needed, and it allows the study of this control mechanism in healthy and pathological aging. To this end, MEG signals were acquired from 32 healthy individuals and 38 MCI patients, both in resting state and while performing a subtraction task of two levels of difficulty. Phase Locking Value (PLV) was calculated for five frequency bands: delta, theta, alpha, beta and gamma. Synchronization patterns changed in both groups while performing the task. MCI patients presented higher connectivity changes than those in the control group, and this was related to a lower cognitive performance. In particular, in MCIs a hypersynchronization in delta, theta, beta and gamma bands was found, which reveals an abnormal functioning in this group. Contrary to controls, MCIs presented a lack of synchronization in the alpha band which may denote an inhibition deficit. Additionally, the magnitude of connectivity changes rose with the task difficulty in controls but not in MCIs, in line with the CRUNCH model (Compensation-Related Utilization of Neural Circuits Hypothesis)

Multicore fiber scenarios supporting power over fiber in radio over fiber systems

We propose the integration of power over fiber in the next generation 5G radio access network front-haul solutions based on spatial division multiplexing with multicore fibers. The different architectures in both shared- and dedicated- core scenarios for power over fiber delivery and data signals are described. The maximum power to be delivered depending on the efficiencies of the different components is addressed as well as the limits of the delivered energy to avoid fiber fuse and non-linear effects. It is shown how those limits depend on high power laser linewidth, fiber attenuation, link length and fiber core effective area. The impairments related to non-linear effects, multicore fiber crosstalk and temperature are also theoretically analyzed. Experiments show there is no degradation of signal quality for feeding powers of several hundreds of milliwatts for both scenarios in 4-core multicore fibers. This study helps in designing future power by light delivery solutions in Radio over Fiber systems with multicore fibers.This work was supported in part by the Spanish Ministry of Science, Innovation and Universities, Directorate for Research and Innovation at Madrid region, and H2020 European Union programme under Grant RTI2018-094669-B-C32 and Grant Y2018/EMT-4892, and in part by FSE and 5G PPP Bluespace project Grant 762055