O Trabalho Do Guincheiro No Porto De Vitória/Es E Os Desafios Do Processo Formativo

This article analyzed the formative process of the Board Crane Driverin the port of Vit ria ES It was used as methodological strategy the creation of a work analysis group as dispositive to data production Through the analyzes the production of a sensible body-guincheiro in the work that emerges as a fundamental arbiter of the choices made that carries the practical knowledge incarnated coming from the activity and that doesn t subdue to the simple execution or simulation of a task either by repetition or protocols stipulated by the work organization in the port but which places itself as the center of activity the locus of rule debate

Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation

An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50–55°C. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.). Ions Ca2+ enhanced the stability of enzyme and its activity by 30%. The Km was 1.30 in absence of Ca2+ and 1.16 mg mL−1 in presence of this ion. In relation to the Vmax the presence of this ion increased from 1.76 to 2.07 μmol min−1mg−1

A GESTÃO DA SALA DE AULA VIRTUAL E OS NOVOS SABERES PARA A DOCÊNCIA NA MODALIDADE DE EDUCAÇÃO A DISTÂNCIA

Este trabalho de natureza qualitativa teve como objetivo investigar dificuldades e desafios encontrados por um grupo de docentes universitários, frente a docência virtual. Os docentes respondentes estavam responsáveis por disciplinas na modalidade à distância de cinco diferentes cursos de graduação de uma Universidade Pública Federal, parceira de um Programa Federal de EaD – UAB (Universidade Aberta do Brasil). A análise apresentada foi baseada em dados coletados por meio de um questionário com questões abertas e fechadas aplicado a 80 professores e respondido por 59 deles (47% mulheres e 53% homens). Apesar das dificuldades apontadas, 100% dos docentes afirmaram que a experiência foi muito positiva. A experiência anterior em educação presencial (69% com mais de 5 anos de experiência) também foi apontada como muito importante para 86% dos docentes. Adequar conteúdos e atividades à EaD, não é tarefa trivial, é necessário tempo e espaço institucional para a discussão de novos caminhos e possibilidades de utilização crítica e consciente das novas mídias, do trabalho coletivo, e do que significa essa mudança de postura na construção do conhecimento

DIGNIDADE DA MULHER E EPISIOTOMIA: contributos de uma análise pautada nas capacidades centrais de Martha Nussbaum

O presente ensaio pretende aferir as circunstâncias nas quais a episiotomia pode ser considerada ofensiva à dignidade das mulheres gestantes. O marco para a reflexão jurídico-dogmática e teórico-descritiva é a ideia de que a dignidade pode ser entendida como sendo delineada por parâmetros mínimos para uma vida digna a partir da Teoria das Capacidades, de Martha Nussbaum. A relevância do estudo é evidenciada por coadunar com lutas e reivindicações feministas. Conclui-se que a dignidade humana tende a ser violada quando da realização de procedimentos não autorizados, desfundamentados cientificamente e pautados em interesses pragmáticos alheios à subjetividade da mulher parturiente

Cytotoxicity analysis of three Bacillus thuringiensis Subsp. israelensis d-Endotoxins towards insect and mammalian cells

Three members of the d-endotoxin group of toxins expressed by Bacillus thuringiensis subsp. israelensis, Cyt2Ba, Cry4Aa and Cry11A, were individually expressed in recombinant acrystalliferous B. thuringiensis strains for in vitro evaluation of their toxic activities against insect and mammalian cell lines. Both Cry4Aa and Cry11A toxins, activated with either trypsin or Spodoptera frugiperda gastric juice (GJ), resulted in different cleavage patterns for the activated toxins as seen by SDS-PAGE. The GJ-processed proteins were not cytotoxic to insect cell cultures. On the other hand, the combination of the trypsinactivated Cry4Aa and Cry11A toxins yielded the highest levels of cytotoxicity to all insect cells tested. The combination of activated Cyt2Ba and Cry11A also showed higher toxic activity than that of toxins activated individually. When activated Cry4Aa, Cry11A and Cyt2Ba were used simultaneously in the same assay a decrease in toxic activity was observed in all insect cells tested. No toxic effect was observed for the trypsin-activated Cry toxins in mammalian cells, but activated Cyt2Ba was toxic to human breast cancer cells (MCF-7) when tested at 20 mg/mL

Immune system challenge improves recognition memory and reverses malaria-induced cognitive impairment in mice

The immune system plays a role in the maintenance of healthy neurocognitive function. Different patterns of immune response triggered by distinct stimuli may affect nervous functions through regulatory or deregulatory signals, depending on the properties of the exogenous immunogens. Here, we investigate the effect of immune stimulation on cognitive-behavioural parameters in healthy mice and its impact on cognitive sequelae resulting from non-severe experimental malaria. We show that immune modulation induced by a specific combination of immune stimuli that induce a type 2 immune response can enhance long-term recognition memory in healthy adult mice subjected to novel object recognition task (NORT) and reverse a lack of recognition ability in NORT and anxiety-like behaviour in a light/dark task that result from a single episode of mild Plasmodium berghei ANKA malaria. Our findings suggest a potential use of immunogens for boosting and recovering recognition memory that may be impaired by chronic and infectious diseases and by the effects of ageing

Association between cardiorespiratory fitness and body fat in girls

AbstractObjectiveTo estimate the prevalence of low cardiorespiratory fitness and its association with excess body fat, considering the sexual maturation and economic level in female adolescents.MethodsCross-sectional, epidemiological study of 1223 adolescents (10–17 years) from the public school system of Cascavel, PR, Brazil, in 2006. We analyzed the self-assessed sexual maturation level (prepubertal, pubertal and post-pubertal), the economic level (high and low) through a questionnaire and body fat (normal and high) through triceps and subscapular skinfolds. The 20-meter back-and-forth test was applied to estimate maximum oxygen consumption. Cardiorespiratory fitness was assessed according to reference criteria and considered low when the minimum health criterion for age and sex was not met. Chi-square test and logistic regression were applied, with a significance level of 5%.ResultsThe prevalence of low cardiorespiratory fitness was 51.3%, being associated with all study variables (p<0.001). At the crude analysis, adolescents with high body fat were associated with low cardiorespiratory fitness, when compared to those with normal body fat (OR=2.76; 95%CI: 2.17–3.52). After adjustment by sexual maturation, this association remained valid and showed an effect that was 1.8-fold higher (95%CI: 1.39–2.46) and after adjusting by economic level, the effect was 1.9-fold higher (95%CI: 1.45–2.61).ConclusionsApproximately half of the assessed girls showed unsatisfactory levels of cardiorespiratory fitness for health, which was associated with high body fat, regardless of sexual maturation level and economic level. Effective public health measures are needed, with particular attention to high-risk groups

Biocompatibility of acetazolamide pastes in the subcutaneous tissue of rats

This aim of this study was to investigate the biocompatibility of two experimental acetazolamide (AZ)-based pastes in the subcutaneous tissue of rats. Both pastes contained AZ as the main component in similar concentration. The vehicle in experimental paste 1 was saline, while experimental paste 2 was prepared with propylene glycol. Sixty polyethylene tubes were sealed at one end with gutta-percha (GP), which served as a control. Half of the tubes were filled with paste 1 and half with paste 2. The tubes were implanted in the subcutaneous tissue of 15 rats, being 4 tubes for each animal. The animals were killed 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin and eosin and were analyzed by light microscopy. Scores were assigned to level of inflammatory process: 1- none; 2- mild; 3- moderate; 4- severe. The data were analyzed statistically by the Kruskal-Wallis test (p&#8804;0.05). Paste 1 produced an inflammatory process at 7 days. However, the intensity of this inflammation decreased with time and was nearly absent at 45 days. No statistically significant difference (p>0.05) was observed between the control (GP) and paste 1. However, paste 2 produced inflammatory response at all study periods and differed significantly (p<0.05) from the control. In conclusion, in the present study, the experimental AZ-based paste 1 was considered as biocompatible as the control matrial (GP), while experimental paste 2 was irritating to rat subcutaneous tissue.Este estudo investigou a biocompatibilidade de pastas experimentais a base de acetazolamida em tecido subcutâneo de rato. Duas pastas foram usadas neste estudo. Ambas continham a acetalozamida como componente principal em concentrações similares. O veículo usado na pasta experimental 1 foi o soro fisiológico e na pasta experimental 2 foi o propilenoglicol. Sessenta tubos de polietileno foram selados em uma das extremidades com guta-percha, que serviu como controle. Metade dos tubos foi preenchida com a pasta 1 e metade com a pasta 2. Os tubos foram introduzidos no tecido subcutâneo de 15 ratos (4 tubos por animal). Aos 7, 15 e 45 dias após a cirurgia, os animais foram sacrificados e os espécimes processados em laboratório. Os cortes histológicos foram corados com hematoxilina e eosina e analisados em microscópio de luz. Escores foram estabelecidos de acordo com a intensidade do processo inflamatório: 1-sem inflamação; 2-discreta; 3-moderada; 4-severa. Os dados obtidos foram comparados estatisticamente através do teste de Kruskal-Wallis (p&#8804;0,05). A pasta 1 promoveu processo inflamatório aos 7 dias. Entretanto, sua intensidade diminuiu com o tempo e estava praticamente ausente aos 45 dias. Não foi observada diferença estatisticamente significante entre o controle (guta-percha) e a pasta 1. Entretanto, a pasta 2 promoveu reação inflamatória em todos os períodos experimentais, com diferença estatisticamente significante em relação ao controle. Assim, a pasta experimental de acetazolamida 1 foi considerada biocompatível como o controle deste trabalho. Já a pasta experimental 2 foi irritante aos tecidos

Avaliação da carga física de trabalho na produção de café: aspectos biomecânicos e fisiológicos

The main objective of this research was the assessment of the physical workload of farm coffee workers from southern Minas Gerais, Brazil. Twelve workers were reconded and their heart rate monitored during one hour execution of five different tasks, both on flat and sloping ground. The assessment of body postures adopted and the actions performed were achieved through the “Captiv” software. We attempted to correlate the results of the assessments and interpret them in the light of the observation of the activities of the workers. The most significant cardiovascular demands occurred in subtasks of foliar and manual fertilization, classifying them as moderate work. Harvesting and thinning were the subtasks that appeared to have the greatest variability of postural combinations: the harvest due to the variety of operating actions; the thinning, although only one relevant operational action, due to the very nature of the task. Either the cardiovascular or the biomechanical indicators revealed no statistically significant differences between the subtasks carried by workers in conditions of flat and sloping ground.Objetivou-se, nesta pesquisa, avaliar a carga física do trabalho na cafeicultura do sul de Minas Gerais, Brasil. Doze trabalhadores foram filmados e sua frequência cardíaca foi monitorada durante uma hora na execução de cinco diferentes tarefas, ambas em condições de terreno plano e em declive. Foram avaliadas as combinações posturais adotadas através do software “Captiv”. Buscou-se correlacionar os resultados das avaliações e interpretá-los à luz da observação das atividades dos trabalhadores. As cargas cardiovasculares mais elevadas ocorreram nas subtarefas de adubação foliar e adubação a lanço, classificando-as como trabalho moderado. A colheita e a desbrota foram as subtarefas que apresentaram maior variabilidade de combinações posturais: a colheita devido à variedade de ações operacionais; a desbrota, apesar de uma única ação operacional relevante, por conta da própria natureza da tarefa. Tanto os indicadores cardiovasculares, como os biomecânicos não revelaram diferenças estatisticamente significativas entre as subtarefas desenvolvidas pelos trabalhadores, em condições de terreno plano e em declive