68 research outputs found
CD1d Expression in Paneth Cells and Rat Exocrine Pancreas Revealed by Novel Monoclonal Antibodies Which Differentially Affect NKT Cell Activation
Background: CD1d is a nonpolymorphic MHC class I-like molecule which presents nonpeptide ligands, e.g. glycolipids, to NKT cells. These cells are known to have multiple effects on innate and adaptive immune responses and on the development of pathological conditions. In order to analyze CD1d expression and function in the rat, the first rat CD1dspecific monoclonal antibodies (mAbs) were generated. Methodology/Principal Findings: Two mAbs, WTH-1 and WTH-2, were generated which bound equally well to cell surfaceexpressed rat and mouse CD1d. Their non-overlapping epitopes were mapped to the CD1d heavy chain. Flow cytometry and immunohistological analyses revealed a nearly identical degree and pattern of CD1d expression for hematopoieitic cells of both species. Notable is also the detection of CD1d protein in mouse and rat Paneth cells as well as the extremely high CD1d expression in acinar exocrine cells of the rat pancreas and the expression of CD4 on rat marginal zone B cells. Both mAbs blocked a-galactosylceramide recognition by primary rat and mouse NKT cells. Interestingly, the two mAbs differed in their impact on the activation of various autoreactive T cell hybridomas, including the XV19.2 hybridoma whose activation was enhanced by the WTH-1 mAb. Conclusions/Significance: The two novel monoclonal antibodies described in this study, allowed the analysis of CD1d expression and CD1d-restricted T cell responses in the rat for the first time. Moreover, they provided new insights into mechanisms of CD1d-restricted antigen recognition. While CD1d expression by hematopoietic cells of mice and rats was extremely similar, CD1d protein was detected at not yet described sites of non-lymphatic tissues such as the rat exocrine pancreas and Paneth cells. The latter is of special relevance given the recently reported defects of Paneth cells in CD1d2/2 mice, which resulted in an altered composition of the gut flora
Recent advances in food allergy
Food allergy is a public health issue that has significantly increased worldwide in the past decade, affecting consumers’ quality of life and making increasing demands on health service resources. Despite recent advances in many areas of diagnosis and treatment, our general knowledge of the basic mechanisms of the disease remain limited i.e., not at pace with the exponential number of new cases and the explosion of new technologies. Many important key questions remain: What defines a major allergen? Why do some individuals develop food allergies and others do not? Which are the environmental factors? Could the environmental factors be monitored through epigenetics or modified by changes in the microbiome? Can tolerance to food be induced? Why are some foods more likely to trigger allergies than others? Does the route and timing of exposure have any role on sensitization? These and many other related questions remain unanswered. In this short review some of these topics are addressed in the light of recent advances in the area
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Habita In Schola Episcopali Martispurgensi ab Andrea Rhostio ..
Den geseegneten Eintritt in das sieben und funffzigste Jahr, Solte Dem Durchlauchtigsten Fürsten und Herrn, Herrn Wilhelm Ernsten, Herzogen zu Sachsen, Jülich, Cleve und Berg ... Meinen gnädigsten regierenden Landes-Fürsten und Herren, Aus unterthänigster Pflicht und Gehorsam an Dero Hochfürstl. Geburths-Tage beglückwünschen Den 30sten Octobr. 1718. M. Friedrich Wilhelm Rhost, Diaconus Substitutus zu Buttstädt
Huldigungsgedicht fĂĽr Wilhelm Ernst, Herzog zu Sachsen-WeimarVorlageform des Erscheinungsvermerks: Wejmar, gedruckt mit Mumbachischen Schrifften
Libri Secundi Politicorum Aristotelis Cum Paraphrasi Heinsiana Emendatius Editorum Disputatio
Quam Praeside M. Severo Christophoro Olpio, Poeseos Ordinario Et Moralium Extraordinario Professore Publico- publice ventilandam proponit d. Febr. Samuel Rostius, Rudolphopoli-Svarzburgicu
De meteoro lucido singulari a. MDCCXXX m. octobri conspecto dissertatio qua observationes Madritensis et Vitembergensis inter se comparantur ... publice proposita Vitembergae d. XXIII. Iunii a. C. MDCCXXXI
a Io. Friderico Weidlero... et Christiano Samuele Rhostio..
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